Reviewed, UniProtKB/Swiss-Prot Q7L590 (MCM10_HUMAN)

Names and origin

Protein names

Recommended name:
    Protein MCM10 homolog
      Short name=HsMCM10

Gene names

Name:	MCM10
ORF Names:	PRO2249

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	875 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Proposed to be involved in DNA replication and to participate in the activation of the pre-replication complex (pre-RC). Temporarily recruited to the replication sites before they replicate and dissociates from chromatin after the activation of the pre-RC. Ref.1 Ref.8
Subunit structure	Self-associates By similarity. Interacts with ORC2. May interact with MCM2 and MCM6.
Subcellular location	Nucleus. Note: Colocalizes with ORC2 in nuclei foci. Associated with chromatin in S phase. From early to mid-S phase located in discrete nuclear foci. In early S phase, several hundred foci appeared throughout the nucleus. In mid-S phase, the foci appeared at the nuclear periphery and nucleolar regions. In the late S and G phases localized to nucleoli. Ref.1 Ref.8
Developmental stage	Expression is cell cycle regulated. Expression increases at the G1/S-boundary. Expression decreases in late M phase, remains low during G1 phase, and starts to accumulate at the onset of S phase. Ref.1 Ref.7
Domain	The zinc finger-like domain binds a zinc ion and is involved in self-association By similarity.
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.7 Ref.10 Ref.11
Sequence similarities	Belongs to the MCM10 family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Zinc-finger
Ligand	Metal-binding Zinc
PTM	Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from Experiment. Source: Reactome
Cellular component	nucleoplasm Ref.1 Inferred from Experiment. Source: Reactome
Molecular function	protein binding Ref.1 Inferred from physical interaction. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CCND1	P24385	1	EBI-374912,EBI-375001
CCND3	P30281	1	EBI-374912,EBI-375013
CDC45L	O75419	1	EBI-374912,EBI-374969
CDC5L	Q99459	1	EBI-374912,EBI-374880
CDC6	Q99741	1	EBI-374912,EBI-374862
CDC7	O00311	1	EBI-374912,EBI-374980
CDK6	Q00534	1	EBI-374912,EBI-295663
CDKN1A	P38936	1	EBI-374912,EBI-375077
CDKN2A	P42771	1	EBI-374912,EBI-375053
MCM2	P49736	1	EBI-374912,EBI-374819
MCM3	P25205	1	EBI-374912,EBI-355153
MCM6	Q14566	1	EBI-374912,EBI-374900
MCM7	P33993	1	EBI-374912,EBI-355924
ORC2L	Q13416	3	EBI-374912,EBI-374957
ORC3L	Q9UBD5	1	EBI-374912,EBI-374916
ORC4L	O43929	1	EBI-374912,EBI-374889

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q7L590-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q7L590-2)

The sequence of this isoform differs from the canonical sequence as follows:
     152-152: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 875	875	Protein MCM10 homolog		PRO_0000278320
Regions
	Region	390 – 415	26	Zinc finger-like
Amino acid modifications
	Modified residue	85	1	Phosphothreonine Ref.10 Ref.11
	Modified residue	93	1	Phosphoserine Ref.10 Ref.11
	Cross-link	627		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.9
Natural variations
	Alternative sequence	152	1	Missing in isoform 2.		VSP_029951
	Natural variant	134	1	K → R: dbSNP rs17152897. Ref.3		VAR_030771
	Natural variant	195	1	A → P: dbSNP rs34630110. Ref.3 Ref.2		VAR_053836
	Natural variant	418	1	A → V: dbSNP rs35114749.		VAR_053837
	Natural variant	541	1	T → S: dbSNP rs7905784. Ref.2 Ref.5		VAR_030772
	Natural variant	669	1	K → R: dbSNP rs2274110. Ref.5		VAR_030773
Experimental info
	Sequence conflict	303	1	K → R in BAB70988. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 20, 2007. Version 2. Checksum: DD87191FFF3729D4	FASTA	875	98,183
	10 20 30 40 50 60 MDEEEDNLSL LTALLEENES ALDCNSEENN FLTRENGEPD AFDELFDADG DGESYTEEAD 70 80 90 100 110 120 DGETGETRDE KENLATLFGD MEDLTDEEEV PASQSTENRV LPAPAPRREK TNEELQEELR 130 140 150 160 170 180 NLQEQMKALQ EQLKVTTIKQ TASPARLQKS PVEKSPRPPL KERRVQRIQE STCFSAELDV 190 200 210 220 230 240 PALPRTKRVA RTPKASPPDP KSSSSRMTSA PSQPLQTISR NKPSGITRGQ IVGTPGSSGE 250 260 270 280 290 300 TTQPICVEAF SGLRLRRPRV SSTEMNKKMT GRKLIRLSQI KEKMAREKLE EIDWVTFGVI 310 320 330 340 350 360 LKKVTPQSVN SGKTFSIWKL NDLRDLTQCV SLFLFGEVHK ALWKTEQGTV VGILNANPMK 370 380 390 400 410 420 PKDGSEEVCL SIDHPQKVLI MGEALDLGTC KAKKKNGEPC TQTVNLRDCE YCQYHVQAQY 430 440 450 460 470 480 KKLSAKRADL QSTFSGGRIP KKFARRGTSL KERLCQDGFY YGGVSSASYA ASIAAAVAPK 490 500 510 520 530 540 KKIQTTLSNL VVKGTNLIIQ ETRQKLGIPQ KSLSCSEEFK ELMDLPTCGA RNLKQHLAKA 550 560 570 580 590 600 TASGIMGSPK PAIKSISASA LLKQQKQRML EMRRRKSEEI QKRFLQSSSE VESPAVPSSS 610 620 630 640 650 660 RQPPAQPPRT GSEFPRLEGA PATMTPKLGR GVLEGDDVLF YDESPPPRPK LSALAEAKKL 670 680 690 700 710 720 AAITKLRAKG QVLTKTNPNS IKKKQKDPQD ILEVKERVEK NTMFSSQAED ELEPARKKRR 730 740 750 760 770 780 EQLAYLESEE FQKILKAKSK HTGILKEAEA EMQERYFEPL VKKEQMEEKM RNIREVKCRV 790 800 810 820 830 840 VTCKTCAYTH FKLLETCVSE QHEYHWHDGV KRFFKCPCGN RSISLDRLPN KHCSNCGLYK 850 860 870 WERDGMLKEK TGPKIGGETL LPRGEEHAKF LNSLK
	Isoform 2. Checksum: 50A89BAF82253B43	FASTA	874	98,084
	10 20 30 40 50 60 MDEEEDNLSL LTALLEENES ALDCNSEENN FLTRENGEPD AFDELFDADG DGESYTEEAD 70 80 90 100 110 120 DGETGETRDE KENLATLFGD MEDLTDEEEV PASQSTENRV LPAPAPRREK TNEELQEELR 130 140 150 160 170 180 NLQEQMKALQ EQLKVTTIKQ TASPARLQKS PEKSPRPPLK ERRVQRIQES TCFSAELDVP 190 200 210 220 230 240 ALPRTKRVAR TPKASPPDPK SSSSRMTSAP SQPLQTISRN KPSGITRGQI VGTPGSSGET 250 260 270 280 290 300 TQPICVEAFS GLRLRRPRVS STEMNKKMTG RKLIRLSQIK EKMAREKLEE IDWVTFGVIL 310 320 330 340 350 360 KKVTPQSVNS GKTFSIWKLN DLRDLTQCVS LFLFGEVHKA LWKTEQGTVV GILNANPMKP 370 380 390 400 410 420 KDGSEEVCLS IDHPQKVLIM GEALDLGTCK AKKKNGEPCT QTVNLRDCEY CQYHVQAQYK 430 440 450 460 470 480 KLSAKRADLQ STFSGGRIPK KFARRGTSLK ERLCQDGFYY GGVSSASYAA SIAAAVAPKK 490 500 510 520 530 540 KIQTTLSNLV VKGTNLIIQE TRQKLGIPQK SLSCSEEFKE LMDLPTCGAR NLKQHLAKAT 550 560 570 580 590 600 ASGIMGSPKP AIKSISASAL LKQQKQRMLE MRRRKSEEIQ KRFLQSSSEV ESPAVPSSSR 610 620 630 640 650 660 QPPAQPPRTG SEFPRLEGAP ATMTPKLGRG VLEGDDVLFY DESPPPRPKL SALAEAKKLA 670 680 690 700 710 720 AITKLRAKGQ VLTKTNPNSI KKKQKDPQDI LEVKERVEKN TMFSSQAEDE LEPARKKRRE 730 740 750 760 770 780 QLAYLESEEF QKILKAKSKH TGILKEAEAE MQERYFEPLV KKEQMEEKMR NIREVKCRVV 790 800 810 820 830 840 TCKTCAYTHF KLLETCVSEQ HEYHWHDGVK RFFKCPCGNR SISLDRLPNK HCSNCGLYKW 850 860 870 ERDGMLKEKT GPKIGGETLL PRGEEHAKFL NSLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The human homolog of Saccharomyces cerevisiae Mcm10 interacts with replication factors and dissociates from nuclease-resistant nuclear structures in G(2) phase." Izumi M., Yanagi K., Mizuno T., Yokoi M., Kawasaki Y., Moon K.Y., Hurwitz J., Yatagai F., Hanaoka F. Nucleic Acids Res. 28:4769-4777(2000) [PubMed: 11095689] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), SUBCELLULAR LOCATION, FUNCTION, DEVELOPMENTAL STAGE, INTERACTION WITH ORC2; MCM2 AND MCM6.
[2]	"Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. , Tampe J., Heubner D., Wambutt R., Korn B., Klein M., Poustka A. Genome Res. 11:422-435(2001) [PubMed: 11230166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANTS PRO-195 AND SER-541. Tissue: Testis.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS ARG-134 AND PRO-195. Tissue: Thymus.
[4]	"The DNA sequence and comparative analysis of human chromosome 10." Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J. , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J. Nature 429:375-381(2004) [PubMed: 15164054] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS SER-541 AND ARG-669. Tissue: Liver, Lymph and Placenta.
[6]	Zhang C., Yu Y., Zhang S., Wei H., Zhang Y., Zhou G., Bi J., Liu M., He F. Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 517-875. Tissue: Fetal liver.
[7]	"Cell cycle-dependent proteolysis and phosphorylation of human Mcm10." Izumi M., Yatagai F., Hanaoka F. J. Biol. Chem. 276:48526-48531(2001) [PubMed: 11602595] [Abstract] Cited for: DEVELOPMENTAL STAGE, PHOSPHORYLATION.
[8]	"Localization of human Mcm10 is spatially and temporally regulated during the S phase." Izumi M., Yatagai F., Hanaoka F. J. Biol. Chem. 279:32569-32577(2004) [PubMed: 15136575] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[9]	"Tryptic digestion of ubiquitin standards reveals an improved strategy for identifying ubiquitinated proteins by mass spectrometry." Denis N.J., Vasilescu J., Lambert J.-P., Smith J.C., Figeys D. Proteomics 7:868-874(2007) [PubMed: 17370265] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-627, MASS SPECTROMETRY.
[10]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-85 AND SER-93, MASS SPECTROMETRY.
[11]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-85 AND SER-93, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB042719 mRNA. Translation: BAB18723.1. AL136840 mRNA. Translation: CAB66774.2. AK055695 mRNA. Translation: BAB70988.1. AK292701 mRNA. Translation: BAF85390.1. AL355355, AL138764 Genomic DNA. Translation: CAI16558.1. AL138764, AL355355 Genomic DNA. Translation: CAI12913.1. BC004876 mRNA. Translation: AAH04876.2. BC101727 mRNA. Translation: AAI01728.1. AF119869 mRNA. Translation: AAF69623.1. Different initiation.
IPI	IPI00164354. IPI00375915.
RefSeq	NP_060988.3. NP_877428.1.
UniGene	Hs.198363
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q7L590. 29 interactions.
PTM databases
PhosphoSite	Q7L590.
Proteomic databases
PRIDE	Q7L590.
Genome annotation databases
Ensembl	ENSG00000065328. Homo sapiens. [Contig view]
GeneID	55388.
KEGG	hsa:55388.
UCSC	uc001ima.1. human. uc001imb.1. human.
Organism-specific databases
GeneCards	GC10P013243.
HGNC	HGNC:18043. MCM10.
MIM	609357. gene.
PharmGKB	PA30689.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	Q7L590.
OMA	Q7L590. KTCAYTH.
Enzyme and pathway databases
Reactome	REACT_152. Cell Cycle, Mitotic. REACT_1538. Cell Cycle Checkpoints. REACT_383. DNA Replication.
Gene expression databases
ArrayExpress	Q7L590.
Bgee	Q7L590.
CleanEx	HS_MCM10.
Family and domain databases
InterPro	IPR015411. Mcm10. IPR015408. Znf_Mcm10/DnaG. [Graphical view]
Pfam	PF09332. Mcm10. 1 hit. PF09329. zf-primase. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	59786.
SOURCE	Search...

Entry information

Entry name

MCM10_HUMAN

Accession

Primary (citable) accession number: Q7L590
Secondary accession number(s): A8K9I6 , Q3MIR3, Q7LD55, Q96GX4, Q96NB6, Q9H0D7, Q9H3P9, Q9P177

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
Last sequence update:	February 20, 2007
Last modified:	July 7, 2009
This is version 43 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)