Reviewed, UniProtKB/Swiss-Prot Q7L576 (CYFP1_HUMAN)

Names and origin

Protein names

Recommended name:
    Cytoplasmic FMR1-interacting protein 1
Alternative name(s):
    Specifically Rac1-associated protein 1
      Short name=Sra-1
    p140sra-1

Gene names

Name:	CYFIP1
Synonyms:	KIAA0068

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1253 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in formation of membrane ruffles and lamellipodia protrusions and in axon outgrowth. Binds to F-actin but not to RNA.
Subunit structure	Interacts with the active GTP-bound form of RAC1. Interacts with FMR1/FMRP but does not bind to related proteins FXR1 or FXR2. Component of the WAVE2 complex composed of ABI1, CYFIP1/SRA1, NCKAP1/NAP1 and WASF2/WAVE2 By similarity. Interacts through its C-terminus with the C-terminus of DPYSL2/CRMP2 which is necessary for DPYSL2-induced axon outgrowth.
Subcellular location	Cytoplasm › perinuclear regionBy similarity. Cell projection › lamellipodiumBy similarity. Cell projection › ruffleBy similarity. Cell junction › synapse › synaptosomeBy similarity. Note= Highly expressed in the perinuclear region. Enriched in synaptosomes, membrane ruffles and at the tips of lamellipodia By similarity.
Sequence similarities	Belongs to the CYFIP family.

Ontologies

Keywords
Biological process	Cell shape Differentiation Neurogenesis
Cellular component	Cell junction Cell projection Cytoplasm Synapse Synaptosome
Coding sequence diversity	Alternative splicing
Ligand	Actin-binding
Molecular function	Developmental protein
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	axon extension Ref.7 Inferred from mutant phenotype. Source: UniProtKB lamellipodium biogenesis Inferred from sequence or structural similarity. Source: UniProtKB regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW ruffle organization Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW lamellipodium Inferred from sequence or structural similarity. Source: UniProtKB perinuclear region of cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB ruffle Inferred from sequence or structural similarity. Source: UniProtKB synapse Inferred from electronic annotation. Source: UniProtKB-KW synaptosome Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	Rac GTPase binding Inferred from sequence or structural similarity. Source: UniProtKB actin filament binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
EIF4E	P06730	1	EBI-1048143,EBI-73440
Eif4e	P63073	1	EBI-1048143,EBI-2000006	From a different organism.
NCKAP1	Q9Y2A7	2	EBI-1048143,EBI-389845

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q7L576-1)

Also known as: 3;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q7L576-2)

Also known as: 4;

The sequence of this isoform differs from the canonical sequence as follows:
     1-431: Missing.
     432-557: AEEYERATRY...PRRAVGPSST → MAESLGSAEL...SPPQMFPWII

Isoform 3 (identifier: Q7L576-3)

Also known as: 5;

The sequence of this isoform differs from the canonical sequence as follows:
     1-806: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1253	1253	Cytoplasmic FMR1-interacting protein 1		PRO_0000279706
Amino acid modifications
	Modified residue	108	1	Phosphotyrosine
	Modified residue	1068	1	Phosphothreonine
Natural variations
	Alternative sequence	1 – 806	806	Missing in isoform 3.		VSP_052345
	Alternative sequence	1 – 431	431	Missing in isoform 2.		VSP_052346
	Alternative sequence	432 – 557	126	AEEYE…GPSST → MAESLGSAELLRQLKSLGME RLLHAVNTFLRQSCTYLPLL TFGGKTSFVSLDVYGTEANC SATSCSFPKAAATWPRRQAP GPLGELVRGPPDQGVAEQSF SHGLFEFGITNVPCIFSPPQ MFPWII in isoform 2.		VSP_052347
Experimental info
	Sequence conflict	583	1	S → N in AAW51478. Ref.1
	Sequence conflict	583	1	S → N in BAC86825. Ref.3
	Sequence conflict	820	1	G → S in AAW51478. Ref.1
	Sequence conflict	820	1	G → S in BAC86825. Ref.3
	Sequence conflict	820	1	G → S in AAH01306. Ref.4
	Sequence conflict	898	1	Y → H in AAW51478. Ref.1
	Sequence conflict	898	1	Y → H in BAC86825. Ref.3
	Sequence conflict	930	1	M → R in AAW51478. Ref.1
	Sequence conflict	930	1	M → R in BAC86825. Ref.3
	Sequence conflict	1176	1	V → A in AAW51479. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (3) [UniParc]. Last modified July 5, 2004. Version 1. Checksum: D8F45E13207BEF16	FASTA	1,253	145,182
	10 20 30 40 50 60 MAAQVTLEDA LSNVDLLEEL PLPDQQPCIE PPPSSLLYQP NFNTNFEDRN AFVTGIARYI 70 80 90 100 110 120 EQATVHSSMN EMLEEGQEYA VMLYTWRSCS RAIPQVKCNE QPNRVEIYEK TVEVLEPEVT 130 140 150 160 170 180 KLMNFMYFQR NAIERFCGEV RRLCHAERRK DFVSEAYLIT LGKFINMFAV LDELKNMKCS 190 200 210 220 230 240 VKNDHSAYKR AAQFLRKMAD PQSIQESQNL SMFLANHNKI TQSLQQQLEV ISGYEELLAD 250 260 270 280 290 300 IVNLCVDYYE NRMYLTPSEK HMLLKVMGFG LYLMDGSVSN IYKLDAKKRI NLSKIDKYFK 310 320 330 340 350 360 QLQVVPLFGD MQIELARYIK TSAHYEENKS RWTCTSSGSS PQYNICEQMI QIREDHMRFI 370 380 390 400 410 420 SELARYSNSE VVTGSGRQEA QKTDAEYRKL FDLALQGLQL LSQWSAHVME VYSWKLVHPT 430 440 450 460 470 480 DKYSNKDCPD SAEEYERATR YNYTSEEKFA LVEVIAMIKG LQVLMGRMES VFNHAIRHTV 490 500 510 520 530 540 YAALQDFSQV TLREPLRQAI KKKKNVIQSV LQAIRKTVCD WETGHEPFND PALRGEKDPK 550 560 570 580 590 600 SGFDIKVPRR AVGPSSTQLY MVRTMLESLI ADKSGSKKTL RSSLEGPTIL DIEKFHRESF 610 620 630 640 650 660 FYTHLINFSE TLQQCCDLSQ LWFREFFLEL TMGRRIQFPI EMSMPWILTD HILETKEASM 670 680 690 700 710 720 MEYVLYSLDL YNDSAHYALT RFNKQFLYDE IEAEVNLCFD QFVYKLADQI FAYYKVMAGS 730 740 750 760 770 780 LLLDKRLRSE CKNQGATIHL PPSNRYETLL KQRHVQLLGR SIDLNRLITQ RVSAAMYKSL 790 800 810 820 830 840 ELAIGRFESE DLTSIVELDG LLEINRMTHK LLSRYLTLDG FDAMFREANH NVSAPYGRIT 850 860 870 880 890 900 LHVFWELNYD FLPNYCYNGS TNRFVRTVLP FSQEFQRDKQ PNAQPQYLHG SKALNLAYSS 910 920 930 940 950 960 IYGSYRNFVG PPHFQVICRL LGYQGIAVVM EELLKVVKSL LQGTILQYVK TLMEVMPKIC 970 980 990 1000 1010 1020 RLPRHEYGSP GILEFFHHQL KDIVEYAELK TVCFQNLREV GNAILFCLLI EQSLSLEEVC 1030 1040 1050 1060 1070 1080 DLLHAAPFQN ILPRVHVKEG ERLDAKMKRL ESKYAPLHLV PLIERLGTPQ QIAIAREGDL 1090 1100 1110 1120 1130 1140 LTKERLCCGL SMFEVILTRI RSFLDDPIWR GPLPSNGVMH VDECVEFHRL WSAMQFVYCI 1150 1160 1170 1180 1190 1200 PVGTHEFTVE QCFGDGLHWA GCMIIVLLGQ QRRFAVLDFC YHLLKVQKHD GKDEIIKNVP 1210 1220 1230 1240 1250 LKKMVERIRK FQILNDEIIT ILDKYLKSGD GEGTPVEHVR CFQPPIHQSL ASS
	Isoform 2 (4) [UniParc]. Checksum: 035E45763DC5F6C4		822	94,467
	10 20 30 40 50 60 MAESLGSAEL LRQLKSLGME RLLHAVNTFL RQSCTYLPLL TFGGKTSFVS LDVYGTEANC 70 80 90 100 110 120 SATSCSFPKA AATWPRRQAP GPLGELVRGP PDQGVAEQSF SHGLFEFGIT NVPCIFSPPQ 130 140 150 160 170 180 MFPWIIQLYM VRTMLESLIA DKSGSKKTLR SSLEGPTILD IEKFHRESFF YTHLINFSET 190 200 210 220 230 240 LQQCCDLSQL WFREFFLELT MGRRIQFPIE MSMPWILTDH ILETKEASMM EYVLYSLDLY 250 260 270 280 290 300 NDSAHYALTR FNKQFLYDEI EAEVNLCFDQ FVYKLADQIF AYYKVMAGSL LLDKRLRSEC 310 320 330 340 350 360 KNQGATIHLP PSNRYETLLK QRHVQLLGRS IDLNRLITQR VSAAMYKSLE LAIGRFESED 370 380 390 400 410 420 LTSIVELDGL LEINRMTHKL LSRYLTLDGF DAMFREANHN VSAPYGRITL HVFWELNYDF 430 440 450 460 470 480 LPNYCYNGST NRFVRTVLPF SQEFQRDKQP NAQPQYLHGS KALNLAYSSI YGSYRNFVGP 490 500 510 520 530 540 PHFQVICRLL GYQGIAVVME ELLKVVKSLL QGTILQYVKT LMEVMPKICR LPRHEYGSPG 550 560 570 580 590 600 ILEFFHHQLK DIVEYAELKT VCFQNLREVG NAILFCLLIE QSLSLEEVCD LLHAAPFQNI 610 620 630 640 650 660 LPRVHVKEGE RLDAKMKRLE SKYAPLHLVP LIERLGTPQQ IAIAREGDLL TKERLCCGLS 670 680 690 700 710 720 MFEVILTRIR SFLDDPIWRG PLPSNGVMHV DECVEFHRLW SAMQFVYCIP VGTHEFTVEQ 730 740 750 760 770 780 CFGDGLHWAG CMIIVLLGQQ RRFAVLDFCY HLLKVQKHDG KDEIIKNVPL KKMVERIRKF 790 800 810 820 QILNDEIITI LDKYLKSGDG EGTPVEHVRC FQPPIHQSLA SS
	Isoform 3 (5) [UniParc]. Checksum: 1D9F2A088E5891A2		447	51,534
	10 20 30 40 50 60 MTHKLLSRYL TLDGFDAMFR EANHNVSAPY GRITLHVFWE LNYDFLPNYC YNGSTNRFVR 70 80 90 100 110 120 TVLPFSQEFQ RDKQPNAQPQ YLHGSKALNL AYSSIYGSYR NFVGPPHFQV ICRLLGYQGI 130 140 150 160 170 180 AVVMEELLKV VKSLLQGTIL QYVKTLMEVM PKICRLPRHE YGSPGILEFF HHQLKDIVEY 190 200 210 220 230 240 AELKTVCFQN LREVGNAILF CLLIEQSLSL EEVCDLLHAA PFQNILPRVH VKEGERLDAK 250 260 270 280 290 300 MKRLESKYAP LHLVPLIERL GTPQQIAIAR EGDLLTKERL CCGLSMFEVI LTRIRSFLDD 310 320 330 340 350 360 PIWRGPLPSN GVMHVDECVE FHRLWSAMQF VYCIPVGTHE FTVEQCFGDG LHWAGCMIIV 370 380 390 400 410 420 LLGQQRRFAV LDFCYHLLKV QKHDGKDEII KNVPLKKMVE RIRKFQILND EIITILDKYL 430 440 KSGDGEGTPV EHVRCFQPPI HQSLASS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Jiang Y.-H., Beaudet A.L. Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3).
[2]	"Prediction of the coding sequences of unidentified human genes. II. The coding sequences of 40 new genes (KIAA0041-KIAA0080) deduced by analysis of cDNA clones from human cell line KG-1." Nomura N., Nagase T., Miyajima N., Sazuka T., Tanaka A., Sato S., Seki N., Kawarabayasi Y., Ishikawa K., Tabata S. DNA Res. 1:223-229(1994) [PubMed: 7584044] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Bone marrow.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain and Placenta.
[5]	Bienvenut W.V., Claeys D., Boldt K., von Kriegsheim A.F., Kolch W. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 50-58; 111-130; 151-163; 366-377; 441-448; 505-515; 564-573; 754-760; 815-826; 867-877; 1054-1076; 1211-1224 AND 1228-1240, MASS SPECTROMETRY. Tissue: B-cell lymphoma and Hepatoma.
[6]	"p140Sra-1 (specifically Rac1-associated protein) is a novel specific target for Rac1 small GTPase." Kobayashi K., Kuroda S., Fukata M., Nakamura T., Nagase T., Nomura N., Matsuura Y., Yoshida-Kubomura N., Iwamatsu A., Kaibuchi K. J. Biol. Chem. 273:291-295(1998) [PubMed: 9417078] [Abstract] Cited for: FUNCTION.
[7]	"CRMP-2 is involved in kinesin-1-dependent transport of the Sra-1/WAVE1 complex and axon formation." Kawano Y., Yoshimura T., Tsuboi D., Kawabata S., Kaneko-Kawano T., Shirataki H., Takenawa T., Kaibuchi K. Mol. Cell. Biol. 25:9920-9935(2005) [PubMed: 16260607] [Abstract] Cited for: FUNCTION, INTERACTION WITH DPYSL2.
[8]	"Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer." Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J., Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L., Mitchell J., Wetzel R., Macneill J., Ren J.M. , Yuan J., Bakalarski C.E., Villen J., Kornhauser J.M., Smith B., Li D., Zhou X., Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J. Cell 131:1190-1203(2007) [PubMed: 18083107] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-108, MASS SPECTROMETRY. Tissue: Lung.
[9]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-1068, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY763577 mRNA. Translation: AAW51476.1. AY763578 mRNA. Translation: AAW51477.1. AY763579 mRNA. Translation: AAW51478.1. AY763580 mRNA. Translation: AAW51479.1. D38549 mRNA. Translation: BAA07552.1. Different initiation. AK127094 mRNA. Translation: BAC86825.1. BC001306 mRNA. Translation: AAH01306.2. BC005097 mRNA. Translation: AAH05097.1.
RefSeq	NP_001028200.1. NP_055423.1.
UniGene	Hs.26704
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q7L576.
PTM databases
PhosphoSite	Q7L576.
Genome annotation databases
Ensembl	ENSG00000068793. Homo sapiens. [Contig view]
GeneID	23191.
KEGG	hsa:23191.
Organism-specific databases
HGNC	HGNC:13759. CYFIP1.
MIM	606322. gene.
Orphanet	72. Angelman syndrome.
PharmGKB	PA38367.
HUGE	Search...
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOVERGEN	Q7L576.
Gene expression databases
ArrayExpress	Q7L576.
CleanEx	HS_CYFIP1.
Family and domain databases
InterPro	IPR008081. Cytoplasmic_FMR1-int. IPR016536. Cytoplasmic_FMR1-int_sub. [Graphical view]
PANTHER	PTHR12195. FragX_IP. 1 hit.
Pfam	PF05994. FragX_IP. 1 hit. [Graphical view]
PIRSF	PIRSF008153. FMR1_interacting. 1 hit.
PRINTS	PR01698. CYTOFMRPINTP.
ProtoNet	Search...
Other Resources
NextBio	44673.
SOURCE	Search...

Entry information

Entry name

CYFP1_HUMAN

Accession

Primary (citable) accession number: Q7L576
Secondary accession number(s): Q14467 , Q5IED0, Q6ZSX1, Q9BSD9, Q9BVC7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 6, 2007
Last sequence update:	July 5, 2004
Last modified:	November 25, 2008
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)