Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Putative ATP-dependent RNA helicase DHX30

Gene

DHX30

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in the assembly of the mitochondrial large ribosomal subunit (PubMed:25683715). Required for optimal function of the zinc-finger antiviral protein ZC3HAV1 (By similarity). Associates with mitochondrial DNA (PubMed:18063578).By similarity2 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi457 – 464ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: GO_Central
  • chromatin binding Source: UniProtKB
  • double-stranded RNA binding Source: MGI
  • RNA binding Source: UniProtKB

GO - Biological processi

  • mitochondrial large ribosomal subunit assembly Source: UniProtKB
  • RNA processing Source: GO_Central

Keywordsi

Molecular functionHelicase, Hydrolase, RNA-binding
Biological processRibosome biogenesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Putative ATP-dependent RNA helicase DHX30 (EC:3.6.4.13)
Alternative name(s):
DEAH box protein 30
Gene namesi
Name:DHX30
Synonyms:DDX30, KIAA0890
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000132153.14.
HGNCiHGNC:16716. DHX30.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Mitochondrion nucleoid

Pathology & Biotechi

Organism-specific databases

DisGeNETi22907.
OpenTargetsiENSG00000132153.
PharmGKBiPA27217.

Polymorphism and mutation databases

BioMutaiDHX30.
DMDMi74758997.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002455381 – 1194Putative ATP-dependent RNA helicase DHX30Add BLAST1194

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei6PhosphoserineCombined sources1
Modified residuei226PhosphoserineBy similarity1
Modified residuei380PhosphoserineCombined sources1
Isoform 2 (identifier: Q7L2E3-2)
Modified residuei15PhosphoserineCombined sources1

Post-translational modificationi

Isoform 2 is phosphorylated on Ser-15.

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ7L2E3.
MaxQBiQ7L2E3.
PaxDbiQ7L2E3.
PeptideAtlasiQ7L2E3.
PRIDEiQ7L2E3.

PTM databases

iPTMnetiQ7L2E3.
PhosphoSitePlusiQ7L2E3.
SwissPalmiQ7L2E3.

Expressioni

Gene expression databases

BgeeiENSG00000132153.
CleanExiHS_DHX30.
ExpressionAtlasiQ7L2E3. baseline and differential.
GenevisibleiQ7L2E3. HS.

Organism-specific databases

HPAiHPA034806.

Interactioni

Subunit structurei

Identified in a complex with TFAM and SSBP1. Interacts with AGO1 and AGO2. Interacts (via N-terminus) with ZC3HAV1 (via N-terminal domain) in an RNA-independent manner (By similarity). Found in a complex with GRSF1, DDX28, FASTKD2 and FASTKD5 (PubMed:25683715).By similarity3 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi116571. 99 interactors.
CORUMiQ7L2E3.
IntActiQ7L2E3. 59 interactors.
MINTiMINT-1186537.
STRINGi9606.ENSP00000405620.

Structurei

Secondary structure

11194
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni47 – 49Combined sources3
Helixi53 – 64Combined sources12
Helixi67 – 73Combined sources7
Beta strandi74 – 78Combined sources5
Beta strandi81 – 91Combined sources11
Beta strandi93 – 95Combined sources3
Beta strandi97 – 105Combined sources9
Helixi106 – 124Combined sources19
Helixi136 – 144Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DB2NMR-A42-147[»]
ProteinModelPortaliQ7L2E3.
SMRiQ7L2E3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7L2E3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini53 – 121DRBMAdd BLAST69
Domaini444 – 612Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST169
Domaini654 – 827Helicase C-terminalPROSITE-ProRule annotationAdd BLAST174

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi559 – 562DEAH box4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi188 – 199Poly-GluAdd BLAST12

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0920. Eukaryota.
COG1643. LUCA.
GeneTreeiENSGT00760000119189.
HOGENOMiHOG000112212.
HOVERGENiHBG081437.
InParanoidiQ7L2E3.
KOiK13185.
OMAiHSNIPMM.
OrthoDBiEOG091G01RZ.
PhylomeDBiQ7L2E3.
TreeFamiTF352030.

Family and domain databases

InterProiView protein in InterPro
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR002464. DNA/RNA_helicase_DEAH_CS.
IPR011709. DUF1605.
IPR007502. Helicase-assoc_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
PfamiView protein in Pfam
PF00270. DEAD. 1 hit.
PF04408. HA2. 1 hit.
PF00271. Helicase_C. 1 hit.
PF07717. OB_NTP_bind. 1 hit.
SMARTiView protein in SMART
SM00487. DEXDc. 1 hit.
SM00847. HA2. 1 hit.
SM00490. HELICc. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS00690. DEAH_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: Q7L2E3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFSLDSFRKD RAQHRQRQCK LPPPRLPPMC VNPTPGGTIS RASRDLLKEF
60 70 80 90 100
PQPKNLLNSV IGRALGISHA KDKLVYVHTN GPKKKKVTLH IKWPKSVEVE
110 120 130 140 150
GYGSKKIDAE RQAAAAACQL FKGWGLLGPR NELFDAAKYR VLADRFGSPA
160 170 180 190 200
DSWWRPEPTM PPTSWRQLNP ESIRPGGPGG LSRSLGREEE EDEEEELEEG
210 220 230 240 250
TIDVTDFLSM TQQDSHAPLR DSRGSSFEMT DDDSAIRALT QFPLPKNLLA
260 270 280 290 300
KVIQIATSSS TAKNLMQFHT VGTKTKLSTL TLLWPCPMTF VAKGRRKAEA
310 320 330 340 350
ENKAAALACK KLKSLGLVDR NNEPLTHAMY NLASLRELGE TQRRPCTIQV
360 370 380 390 400
PEPILRKIET FLNHYPVESS WIAPELRLQS DDILPLGKDS GPLSDPITGK
410 420 430 440 450
PYVPLLEAEE VRLSQSLLEL WRRRGPVWQE APQLPVDPHR DTILNAIEQH
460 470 480 490 500
PVVVISGDTG CGKTTRIPQL LLERYVTEGR GARCNVIITQ PRRISAVSVA
510 520 530 540 550
QRVSHELGPS LRRNVGFQVR LESKPPSRGG ALLFCTVGIL LRKLQSNPSL
560 570 580 590 600
EGVSHVIVDE VHERDVNTDF LLILLKGLQR LNPALRLVLM SATGDNERFS
610 620 630 640 650
RYFGGCPVIK VPGFMYPVKE HYLEDILAKL GKHQYLHRHR HHESEDECAL
660 670 680 690 700
DLDLVTDLVL HIDARGEPGG ILCFLPGWQE IKGVQQRLQE ALGMHESKYL
710 720 730 740 750
ILPVHSNIPM MDQKAIFQQP PVGVRKIVLA TNIAETSITI NDIVHVVDSG
760 770 780 790 800
LHKEERYDLK TKVSCLETVW VSRANVIQRR GRAGRCQSGF AYHLFPRSRL
810 820 830 840 850
EKMVPFQVPE ILRTPLENLV LQAKIHMPEK TAVEFLSKAV DSPNIKAVDE
860 870 880 890 900
AVILLQEIGV LDQREYLTTL GQRLAHISTD PRLAKAIVLA AIFRCLHPLL
910 920 930 940 950
VVVSCLTRDP FSSSLQNRAE VDKVKALLSH DSGSDHLAFV RAVAGWEEVL
960 970 980 990 1000
RWQDRSSREN YLEENLLYAP SLRFIHGLIK QFSENIYEAF LVGKPSDCTL
1010 1020 1030 1040 1050
ASAQCNEYSE EEELVKGVLM AGLYPNLIQV RQGKVTRQGK FKPNSVTYRT
1060 1070 1080 1090 1100
KSGNILLHKS TINREATRLR SRWLTYFMAV KSNGSVFVRD SSQVHPLAVL
1110 1120 1130 1140 1150
LLTDGDVHIR DDGRRATISL SDSDLLRLEG DSRTVRLLKE LRRALGRMVE
1160 1170 1180 1190
RSLRSELAAL PPSVQEEHGQ LLALLAELLR GPCGSFDVRK TADD
Length:1,194
Mass (Da):133,938
Last modified:July 5, 2004 - v1
Checksum:i33D9A08799FE7A02
GO
Isoform 2 (identifier: Q7L2E3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-41: MFSLDSFRKD...NPTPGGTISR → MAAARRLMAL...PGEGDGSLVN

Show »
Length:1,222
Mass (Da):136,115
Checksum:iBEEE9D1C88DAFCA7
GO
Isoform 3 (identifier: Q7L2E3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-39: Missing.
     40-41: SR → MA

Show »
Length:1,155
Mass (Da):129,438
Checksum:iD3417B4C609F6360
GO

Sequence cautioni

The sequence BAA74913 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAA92071 differs from that shown. Probable cloning artifact.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti557I → T in BAF83955 (PubMed:14702039).Curated1
Sequence conflicti781G → D in BAF83955 (PubMed:14702039).Curated1
Sequence conflicti1136R → W in AAH14237 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0221181 – 41MFSLD…GTISR → MAAARRLMALAAGISPRLQP LGPRAAGRQGRSRGFSSSCA HPDHTKEAAEAESGMAPGGP GEGDGSLVN in isoform 2. 1 PublicationAdd BLAST41
Alternative sequenceiVSP_0368911 – 39Missing in isoform 3. 2 PublicationsAdd BLAST39
Alternative sequenceiVSP_03689240 – 41SR → MA in isoform 3. 2 Publications2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB020697 mRNA. Translation: BAA74913.2. Different initiation.
AK002076 mRNA. Translation: BAA92071.1. Sequence problems.
AK291266 mRNA. Translation: BAF83955.1.
BC014237 mRNA. Translation: AAH14237.1.
BC015029 mRNA. Translation: AAH15029.1.
BC020126 mRNA. Translation: AAH20126.1.
BC038417 mRNA. Translation: AAH38417.1.
CCDSiCCDS2759.1. [Q7L2E3-1]
PIRiE56236.
RefSeqiNP_055781.2. NM_014966.3. [Q7L2E3-3]
NP_619520.1. NM_138615.2. [Q7L2E3-1]
XP_011531796.1. XM_011533494.2. [Q7L2E3-1]
XP_011531797.1. XM_011533495.1. [Q7L2E3-1]
XP_016861406.1. XM_017005917.1. [Q7L2E3-3]
UniGeneiHs.517948.

Genome annotation databases

EnsembliENST00000445061; ENSP00000405620; ENSG00000132153. [Q7L2E3-1]
ENST00000446256; ENSP00000392601; ENSG00000132153. [Q7L2E3-1]
ENST00000457607; ENSP00000394682; ENSG00000132153. [Q7L2E3-2]
ENST00000619982; ENSP00000483160; ENSG00000132153. [Q7L2E3-3]
GeneIDi22907.
KEGGihsa:22907.
UCSCiuc003cru.4. human. [Q7L2E3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDHX30_HUMAN
AccessioniPrimary (citable) accession number: Q7L2E3
Secondary accession number(s): A8K5F1
, O94965, Q7Z753, Q96CH4, Q9NUQ0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: July 5, 2004
Last modified: September 27, 2017
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families