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Protein

Carbohydrate sulfotransferase 9

Gene

CHST9

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the transfer of sulfate to position 4 of non-reducing N-acetylgalactosamine (GalNAc) residues in both N-glycans and O-glycans. Participates in biosynthesis of glycoprotein hormones lutropin and thyrotropin, by mediating sulfation of their carbohydrate structures. Has a higher activity toward carbonic anhydrase VI than toward lutropin. Only active against terminal GalNAcbeta1,GalNAcbeta. Isoform 2, but not isoform 1, is active toward chondroitin.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi220 – 2267PAPSBy similarity
Nucleotide bindingi280 – 2889PAPSBy similarity

GO - Molecular functioni

  1. N-acetylgalactosamine 4-O-sulfotransferase activity Source: UniProtKB

GO - Biological processi

  1. carbohydrate biosynthetic process Source: InterPro
  2. carbohydrate metabolic process Source: Reactome
  3. chondroitin sulfate biosynthetic process Source: Reactome
  4. chondroitin sulfate metabolic process Source: Reactome
  5. glycosaminoglycan metabolic process Source: UniProtKB
  6. hormone biosynthetic process Source: UniProtKB
  7. pathogenesis Source: Reactome
  8. proteoglycan biosynthetic process Source: UniProtKB
  9. small molecule metabolic process Source: Reactome
  10. sulfur compound metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

ReactomeiREACT_120989. Chondroitin sulfate biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbohydrate sulfotransferase 9 (EC:2.8.2.-)
Alternative name(s):
GalNAc-4-O-sulfotransferase 2
Short name:
GalNAc-4-ST2
Short name:
GalNAc4ST-2
N-acetylgalactosamine-4-O-sulfotransferase 2
Gene namesi
Name:CHST9
ORF Names:UNQ2549/PRO6175
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 18

Organism-specific databases

HGNCiHGNC:19898. CHST9.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1212CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei13 – 3321Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini34 – 443410LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
  2. Golgi membrane Source: Reactome
  3. integral component of membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane, Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134888782.

Polymorphism and mutation databases

BioMutaiCHST9.
DMDMi229462829.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 443443Carbohydrate sulfotransferase 9PRO_0000189655Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi159 – 1591N-linked (GlcNAc...)Sequence Analysis
Glycosylationi243 – 2431N-linked (GlcNAc...)Sequence Analysis
Glycosylationi324 – 3241N-linked (GlcNAc...)Sequence Analysis
Glycosylationi437 – 4371N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ7L1S5.
PRIDEiQ7L1S5.

PTM databases

PhosphoSiteiQ7L1S5.

Expressioni

Tissue specificityi

Highly expressed in trachea. Also expressed in fetal lung, adult pancreas, testis and salivary gland. Expressed at low level in pituitary gland, apex of the heart, adult lung, prostate and mammary gland. Weakly or not expressed in heart, liver and spinal cord.2 Publications

Gene expression databases

BgeeiQ7L1S5.
CleanExiHS_CHST9.
ExpressionAtlasiQ7L1S5. baseline and differential.
GenevestigatoriQ7L1S5.

Organism-specific databases

HPAiHPA011393.

Interactioni

Protein-protein interaction databases

BioGridi123672. 4 interactions.
STRINGi9606.ENSP00000392091.

Structurei

3D structure databases

ProteinModelPortaliQ7L1S5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the sulfotransferase 2 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG296625.
GeneTreeiENSGT00760000119214.
HOGENOMiHOG000231801.
HOVERGENiHBG050950.
InParanoidiQ7L1S5.
KOiK09673.
OMAiNAQVVRQ.
OrthoDBiEOG7B05D8.
PhylomeDBiQ7L1S5.
TreeFamiTF325581.

Family and domain databases

InterProiIPR018011. Carb_sulfotransferase-rel.
IPR005331. Sulfotransferase.
[Graphical view]
PANTHERiPTHR12137. PTHR12137. 1 hit.
PfamiPF03567. Sulfotransfer_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7L1S5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQPSEMVMNP KQVFLSVLIF GVAGLLLFMY LQVWIEEQHT GRVEKRREQK
60 70 80 90 100
VTSGWGPVKY LRPVPRIMST EKIQEHITNQ NPKFHMPEDV REKKENLLLN
110 120 130 140 150
SERSTRLLTK TSHSQGGDQA LSKSTGSPTE KLIEKRQGAK TVFNKFSNMN
160 170 180 190 200
WPVDIHPLNK SLVKDNKWKK TEETQEKRRS FLQEFCKKYG GVSHHQSHLF
210 220 230 240 250
HTVSRIYVED KHKILYCEVP KAGCSNWKRI LMVLNGLASS AYNISHNAVH
260 270 280 290 300
YGKHLKKLDS FDLKGIYTRL NTYTKAVFVR DPMERLVSAF RDKFEHPNSY
310 320 330 340 350
YHPVFGKAII KKYRPNACEE ALINGSGVKF KEFIHYLLDS HRPVGMDIHW
360 370 380 390 400
EKVSKLCYPC LINYDFVGKF ETLEEDANYF LQMIGAPKEL KFPNFKDRHS
410 420 430 440
SDERTNAQVV RQYLKDLTRT ERQLIYDFYY LDYLMFNYTT PFL
Length:443
Mass (Da):52,055
Last modified:May 5, 2009 - v2
Checksum:i5455E67D20C9F8D6
GO
Isoform 2 (identifier: Q7L1S5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     68-74: MSTEKIQ → KPQVSHA
     75-443: Missing.

Show »
Length:74
Mass (Da):8,591
Checksum:i1F1A2CBAD277FA16
GO

Sequence cautioni

The sequence AAH25764.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAK01862.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti136 – 1361R → H in AAK01862 (PubMed:11445554).Curated
Sequence conflicti150 – 1501N → D in AAK01862 (PubMed:11445554).Curated
Sequence conflicti194 – 1952HH → PP in AAK30370 (PubMed:11139592).Curated
Sequence conflicti442 – 4421F → L in AAQ88852 (PubMed:12975309).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti122 – 1221S → N.
Corresponds to variant rs17694469 [ dbSNP | Ensembl ].
VAR_055150

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei68 – 747MSTEKIQ → KPQVSHA in isoform 2. 1 PublicationVSP_043865
Alternative sequencei75 – 443369Missing in isoform 2. 1 PublicationVSP_043866Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF239821 mRNA. Translation: AAK01862.1. Different initiation.
AF332472 mRNA. Translation: AAK30369.1.
AF332473 mRNA. Translation: AAK30370.1. Sequence problems.
AY358488 mRNA. Translation: AAQ88852.1.
AK093349 mRNA. Translation: BAC04141.1.
AC010854 Genomic DNA. No translation available.
AC023575 Genomic DNA. No translation available.
AC116017 Genomic DNA. No translation available.
BC025764 mRNA. Translation: AAH25764.2. Different initiation.
CCDSiCCDS42422.1. [Q7L1S5-1]
CCDS58618.1. [Q7L1S5-2]
RefSeqiNP_001243245.1. NM_001256316.1. [Q7L1S5-2]
NP_113610.2. NM_031422.5. [Q7L1S5-1]
UniGeneiHs.44584.
Hs.706552.

Genome annotation databases

EnsembliENST00000580774; ENSP00000464655; ENSG00000154080. [Q7L1S5-2]
ENST00000581714; ENSP00000462852; ENSG00000154080. [Q7L1S5-1]
ENST00000618847; ENSP00000480991; ENSG00000154080. [Q7L1S5-1]
GeneIDi83539.
KEGGihsa:83539.
UCSCiuc002kwd.4. human. [Q7L1S5-1]
uc021uij.2. human. [Q7L1S5-2]

Polymorphism and mutation databases

BioMutaiCHST9.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF239821 mRNA. Translation: AAK01862.1. Different initiation.
AF332472 mRNA. Translation: AAK30369.1.
AF332473 mRNA. Translation: AAK30370.1. Sequence problems.
AY358488 mRNA. Translation: AAQ88852.1.
AK093349 mRNA. Translation: BAC04141.1.
AC010854 Genomic DNA. No translation available.
AC023575 Genomic DNA. No translation available.
AC116017 Genomic DNA. No translation available.
BC025764 mRNA. Translation: AAH25764.2. Different initiation.
CCDSiCCDS42422.1. [Q7L1S5-1]
CCDS58618.1. [Q7L1S5-2]
RefSeqiNP_001243245.1. NM_001256316.1. [Q7L1S5-2]
NP_113610.2. NM_031422.5. [Q7L1S5-1]
UniGeneiHs.44584.
Hs.706552.

3D structure databases

ProteinModelPortaliQ7L1S5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi123672. 4 interactions.
STRINGi9606.ENSP00000392091.

PTM databases

PhosphoSiteiQ7L1S5.

Polymorphism and mutation databases

BioMutaiCHST9.
DMDMi229462829.

Proteomic databases

PaxDbiQ7L1S5.
PRIDEiQ7L1S5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000580774; ENSP00000464655; ENSG00000154080. [Q7L1S5-2]
ENST00000581714; ENSP00000462852; ENSG00000154080. [Q7L1S5-1]
ENST00000618847; ENSP00000480991; ENSG00000154080. [Q7L1S5-1]
GeneIDi83539.
KEGGihsa:83539.
UCSCiuc002kwd.4. human. [Q7L1S5-1]
uc021uij.2. human. [Q7L1S5-2]

Organism-specific databases

CTDi83539.
GeneCardsiGC18M024495.
HGNCiHGNC:19898. CHST9.
HPAiHPA011393.
MIMi610191. gene.
neXtProtiNX_Q7L1S5.
PharmGKBiPA134888782.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG296625.
GeneTreeiENSGT00760000119214.
HOGENOMiHOG000231801.
HOVERGENiHBG050950.
InParanoidiQ7L1S5.
KOiK09673.
OMAiNAQVVRQ.
OrthoDBiEOG7B05D8.
PhylomeDBiQ7L1S5.
TreeFamiTF325581.

Enzyme and pathway databases

ReactomeiREACT_120989. Chondroitin sulfate biosynthesis.

Miscellaneous databases

GenomeRNAii83539.
NextBioi72449.
PROiQ7L1S5.
SOURCEiSearch...

Gene expression databases

BgeeiQ7L1S5.
CleanExiHS_CHST9.
ExpressionAtlasiQ7L1S5. baseline and differential.
GenevestigatoriQ7L1S5.

Family and domain databases

InterProiIPR018011. Carb_sulfotransferase-rel.
IPR005331. Sulfotransferase.
[Graphical view]
PANTHERiPTHR12137. PTHR12137. 1 hit.
PfamiPF03567. Sulfotransfer_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and expression of two distinct human N-acetylgalactosamine 4-O-sulfotransferases that transfer sulfate to GalNAc beta 1->4GlcNAc beta 1->R in both N- and O-glycans."
    Hiraoka N., Misra A., Belot F., Hindsgaul O., Fukuda M.
    Glycobiology 11:495-504(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ENZYME ACTIVITY, TISSUE SPECIFICITY.
  2. "Molecular cloning and expression of an N-acetylgalactosamine-4-O-sulfotransferase that transfers sulfate to terminal and non-terminal beta 1,4-linked N-acetylgalactosamine."
    Kang H.-G., Evers M.R., Xia G., Baenziger J.U., Schachner M.
    J. Biol. Chem. 276:10861-10869(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), ENZYME ACTIVITY, TISSUE SPECIFICITY.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Testis.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Lung.

Entry informationi

Entry nameiCHST9_HUMAN
AccessioniPrimary (citable) accession number: Q7L1S5
Secondary accession number(s): Q6UX69
, Q9BXH3, Q9BXH4, Q9BZW9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: May 5, 2009
Last modified: April 29, 2015
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-8 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 18
    Human chromosome 18: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.