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Protein

Rho-related GTP-binding protein RhoU

Gene

RHOU

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts upstream of PAK1 to regulate the actin cytoskeleton, adhesion turnover and increase cell migration. Stimulates quiescent cells to reenter the cell cycle. Has no detectable GTPase activity but its high intrinsic guanine nucleotide exchange activity suggests it is constitutively GTP-bound. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape.5 Publications

Cofactori

Mg2+1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi56 – 63GTP1 Publication8
Nucleotide bindingi103 – 107GTPBy similarity5
Nucleotide bindingi161 – 164GTP4

GO - Molecular functioni

GO - Biological processi

  • actin cytoskeleton organization Source: Ensembl
  • cytokine-mediated signaling pathway Source: Reactome
  • cytoskeleton organization Source: UniProtKB
  • G1/S transition of mitotic cell cycle Source: Ensembl
  • positive regulation of protein targeting to mitochondrion Source: ParkinsonsUK-UCL
  • Rac protein signal transduction Source: Ensembl
  • regulation of cell shape Source: UniProtKB
  • regulation of small GTPase mediated signal transduction Source: Reactome

Keywordsi

LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-194840 Rho GTPase cycle
R-HSA-6785807 Interleukin-4 and 13 signaling
SignaLinkiQ7L0Q8

Names & Taxonomyi

Protein namesi
Recommended name:
Rho-related GTP-binding protein RhoU
Alternative name(s):
CDC42-like GTPase 1
GTP-binding protein-like 1
Rho GTPase-like protein ARHU
Ryu GTPase
Wnt-1 responsive Cdc42 homolog 1
Short name:
WRCH-1
Gene namesi
Name:RHOUImported
Synonyms:ARHUImported, CDC42L1Imported, G28KImported, WRCH1Imported
ORF Names:SB128
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000116574.5
HGNCiHGNC:17794 RHOU
MIMi606366 gene
neXtProtiNX_Q7L0Q8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi63T → N: Loss of GTP-binding and localization to focal adhesions. No effect on ARHGAP30-binding. 3 Publications1
Mutagenesisi80P → G: No effect on ARHGAP30-binding. 1 Publication1
Mutagenesisi81T → S: Loss of binding to PAK3; when associated with A-83 and C-86. 1 Publication1
Mutagenesisi83F → A: Loss of binding to PAK3; when associated with S-81 and C-86. 2 Publications1
Mutagenesisi83F → G: Loss of ARHGAP30-binding. 2 Publications1
Mutagenesisi86F → C: Loss of PAK3-binding; when associated with S-81 and A-83. No effect on ARHGAP30-binding. 2 Publications1
Mutagenesisi107Q → L: Constitutively active. Results in increased rates of stress fiber dissolution and cell migration. No effect on ARHGAP30-binding. 3 Publications1
Mutagenesisi255C → S: No effect on subcellular location. 1 Publication1
Mutagenesisi256C → S: Loss of subcellular location to plasma and intracellular membranes. 1 Publication1

Organism-specific databases

DisGeNETi58480
OpenTargetsiENSG00000116574
PharmGKBiPA38246

Polymorphism and mutation databases

BioMutaiRHOU
DMDMi172046189

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003264351 – 258Rho-related GTP-binding protein RhoUAdd BLAST258

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi256S-palmitoyl cysteine1 Publication1

Post-translational modificationi

Tyrosine phosphorylated by SRC in response to PTK2B/PYK2 activation.1 Publication

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiQ7L0Q8
PeptideAtlasiQ7L0Q8
PRIDEiQ7L0Q8

PTM databases

iPTMnetiQ7L0Q8
PhosphoSitePlusiQ7L0Q8
SwissPalmiQ7L0Q8

Expressioni

Tissue specificityi

Ubiquitously expressed in all tissues examined. Expressed at high levels in the stomach, small intestine, brain, skeletal muscle and placenta.2 Publications

Gene expression databases

BgeeiENSG00000116574
CleanExiHS_RHOU
ExpressionAtlasiQ7L0Q8 baseline and differential
GenevisibleiQ7L0Q8 HS

Organism-specific databases

HPAiHPA049592

Interactioni

Subunit structurei

Interacts with PAK3. Interacts with ARHGAP30 in a GTP-independent manner. In its GTP-loaded conformation, interacts with ARHGAP31. Interacts with PTK2B/PYK2.4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi121812, 127 interactors
IntActiQ7L0Q8, 8 interactors
MINTiQ7L0Q8
STRINGi9606.ENSP00000355652

Structurei

Secondary structure

1258
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi50 – 55Combined sources6
Helixi62 – 70Combined sources9
Beta strandi82 – 92Combined sources11
Beta strandi95 – 103Combined sources9
Beta strandi111 – 113Combined sources3
Helixi114 – 118Combined sources5
Beta strandi122 – 129Combined sources8
Helixi133 – 141Combined sources9
Helixi143 – 150Combined sources8
Beta strandi152 – 154Combined sources3
Beta strandi156 – 161Combined sources6
Helixi163 – 167Combined sources5
Helixi169 – 176Combined sources8
Turni177 – 179Combined sources3
Helixi185 – 195Combined sources11
Beta strandi198 – 202Combined sources5
Turni205 – 207Combined sources3
Helixi211 – 226Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2Q3HX-ray1.73A32-230[»]
ProteinModelPortaliQ7L0Q8
SMRiQ7L0Q8
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7L0Q8

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi26 – 61Gly-richSequence analysisAdd BLAST36

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rho family.1 Publication

Phylogenomic databases

eggNOGiKOG0393 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00760000118978
HOGENOMiHOG000233974
HOVERGENiHBG009351
InParanoidiQ7L0Q8
KOiK07865
OMAiPLCYTNA
OrthoDBiEOG091G0E4F
PhylomeDBiQ7L0Q8
TreeFamiTF321839

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR003578 Small_GTPase_Rho
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51420 RHO, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 17 Publications (identifier: Q7L0Q8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPPQQGDPAF PDRCEAPPVP PRRERGGRGG RGPGEPGGRG RAGGAEGRGV
60 70 80 90 100
KCVLVGDGAV GKTSLVVSYT TNGYPTEYIP TAFDNFSAVV SVDGRPVRLQ
110 120 130 140 150
LCDTAGQDEF DKLRPLCYTN TDIFLLCFSV VSPSSFQNVS EKWVPEIRCH
160 170 180 190 200
CPKAPIILVG TQSDLREDVK VLIELDKCKE KPVPEEAAKL CAEEIKAASY
210 220 230 240 250
IECSALTQKN LKEVFDAAIV AGIQYSDTQQ QPKKSKSRTP DKMKNLSKSW

WKKYCCFV
Length:258
Mass (Da):28,218
Last modified:February 5, 2008 - v1
Checksum:iD90059DE82288B97
GO
Isoform 2 (identifier: Q7L0Q8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-61: Missing.
     62-66: KTSLV → MTNIG

Note: No experimental confirmation available.Curated
Show »
Length:197
Mass (Da):22,164
Checksum:iCAE1A9715F8B7D0B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1 – 13MPPQQ…AFPDR → QLLPTATLRGGGAVGPGPAS PRPQA in BAD92748 (Ref. 7) CuratedAdd BLAST13
Sequence conflicti71T → P in AAL99390 (PubMed:11894124).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_051975121T → A. Corresponds to variant dbSNP:rs3820264Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0527321 – 61Missing in isoform 2. 1 PublicationAdd BLAST61
Alternative sequenceiVSP_05273362 – 66KTSLV → MTNIG in isoform 2. 1 Publication5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF378087 mRNA Translation: AAK83340.1
AB074878 mRNA Translation: BAB86361.1
AB051826 mRNA Translation: BAB18638.1
DQ384420 Genomic DNA Translation: ABD48870.1
DQ384421 Genomic DNA Translation: ABD48871.1
DQ384422 Genomic DNA Translation: ABD48872.1
DQ384423 Genomic DNA Translation: ABD48873.1
DQ384424 Genomic DNA Translation: ABD48874.1
DQ384425 Genomic DNA Translation: ABD48875.1
AF211836 mRNA Translation: AAL54874.1
AF282258 mRNA Translation: AAG46058.1
AF251701 mRNA Translation: AAL99390.1
AK289971 mRNA Translation: BAF82660.1
AB209511 mRNA Translation: BAD92748.1
AL096776 Genomic DNA No translation available.
CH471098 Genomic DNA Translation: EAW69885.1
BC040076 mRNA Translation: AAH40076.1
CCDSiCCDS1575.1 [Q7L0Q8-1]
RefSeqiNP_067028.1, NM_021205.5 [Q7L0Q8-1]
UniGeneiHs.647774

Genome annotation databases

EnsembliENST00000366691; ENSP00000355652; ENSG00000116574 [Q7L0Q8-1]
GeneIDi58480
KEGGihsa:58480
UCSCiuc001htf.3 human [Q7L0Q8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiRHOU_HUMAN
AccessioniPrimary (citable) accession number: Q7L0Q8
Secondary accession number(s): B1AKN1, Q59FE9, Q8TDQ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: February 5, 2008
Last modified: March 28, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health