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Protein

Rho-related GTP-binding protein RhoU

Gene

RHOU

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts upstream of PAK1 to regulate the actin cytoskeleton, adhesion turnover and increase cell migration. Stimulates quiescent cells to reenter the cell cycle. Has no detectable GTPase activity but its high intrinsic guanine nucleotide exchange activity suggests it is constitutively GTP-bound. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape.5 Publications

Cofactori

Mg2+1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi56 – 63GTP1 Publication8
Nucleotide bindingi103 – 107GTPBy similarity5
Nucleotide bindingi161 – 164GTP4

GO - Molecular functioni

GO - Biological processi

  • actin cytoskeleton organization Source: Ensembl
  • cytoskeleton organization Source: UniProtKB
  • G1/S transition of mitotic cell cycle Source: Ensembl
  • positive regulation of protein targeting to mitochondrion Source: ParkinsonsUK-UCL
  • Rac protein signal transduction Source: Ensembl
  • regulation of cell shape Source: UniProtKB
  • regulation of small GTPase mediated signal transduction Source: Reactome
Complete GO annotation...

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-194840. Rho GTPase cycle.
SignaLinkiQ7L0Q8.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho-related GTP-binding protein RhoU
Alternative name(s):
CDC42-like GTPase 1
GTP-binding protein-like 1
Rho GTPase-like protein ARHU
Ryu GTPase
Wnt-1 responsive Cdc42 homolog 1
Short name:
WRCH-1
Gene namesi
Name:RHOUImported
Synonyms:ARHUImported, CDC42L1Imported, G28KImported, WRCH1Imported
ORF Names:SB128
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:17794. RHOU.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi63T → N: Loss of GTP-binding and localization to focal adhesions. No effect on ARHGAP30-binding. 3 Publications1
Mutagenesisi80P → G: No effect on ARHGAP30-binding. 1 Publication1
Mutagenesisi81T → S: Loss of binding to PAK3; when associated with A-83 and C-86. 1 Publication1
Mutagenesisi83F → A: Loss of binding to PAK3; when associated with S-81 and C-86. 2 Publications1
Mutagenesisi83F → G: Loss of ARHGAP30-binding. 2 Publications1
Mutagenesisi86F → C: Loss of PAK3-binding; when associated with S-81 and A-83. No effect on ARHGAP30-binding. 2 Publications1
Mutagenesisi107Q → L: Constitutively active. Results in increased rates of stress fiber dissolution and cell migration. No effect on ARHGAP30-binding. 3 Publications1
Mutagenesisi255C → S: No effect on subcellular location. 1 Publication1
Mutagenesisi256C → S: Loss of subcellular location to plasma and intracellular membranes. 1 Publication1

Organism-specific databases

DisGeNETi58480.
OpenTargetsiENSG00000116574.
PharmGKBiPA38246.

Polymorphism and mutation databases

BioMutaiRHOU.
DMDMi172046189.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003264351 – 258Rho-related GTP-binding protein RhoUAdd BLAST258

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi256S-palmitoyl cysteine1 Publication1

Post-translational modificationi

Tyrosine phosphorylated by SRC in response to PTK2B/PYK2 activation.1 Publication

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

EPDiQ7L0Q8.
PaxDbiQ7L0Q8.
PeptideAtlasiQ7L0Q8.
PRIDEiQ7L0Q8.

PTM databases

iPTMnetiQ7L0Q8.
PhosphoSitePlusiQ7L0Q8.
SwissPalmiQ7L0Q8.

Expressioni

Tissue specificityi

Ubiquitously expressed in all tissues examined. Expressed at high levels in the stomach, small intestine, brain, skeletal muscle and placenta.2 Publications

Gene expression databases

BgeeiENSG00000116574.
CleanExiHS_RHOU.
ExpressionAtlasiQ7L0Q8. baseline and differential.
GenevisibleiQ7L0Q8. HS.

Organism-specific databases

HPAiHPA049592.

Interactioni

Subunit structurei

Interacts with PAK3. Interacts with ARHGAP30 in a GTP-independent manner. In its GTP-loaded conformation, interacts with ARHGAP31. Interacts with PTK2B/PYK2.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NCK2O436396EBI-1638043,EBI-713635
PAK1Q13153-22EBI-1638043,EBI-1019502
PTK2BQ142894EBI-1638043,EBI-298640

Protein-protein interaction databases

BioGridi121812. 127 interactors.
IntActiQ7L0Q8. 4 interactors.
MINTiMINT-7136717.
STRINGi9606.ENSP00000355652.

Structurei

Secondary structure

1258
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi50 – 55Combined sources6
Helixi62 – 70Combined sources9
Beta strandi82 – 92Combined sources11
Beta strandi95 – 103Combined sources9
Beta strandi111 – 113Combined sources3
Helixi114 – 118Combined sources5
Beta strandi122 – 129Combined sources8
Helixi133 – 141Combined sources9
Helixi143 – 150Combined sources8
Beta strandi152 – 154Combined sources3
Beta strandi156 – 161Combined sources6
Helixi163 – 167Combined sources5
Helixi169 – 176Combined sources8
Turni177 – 179Combined sources3
Helixi185 – 195Combined sources11
Beta strandi198 – 202Combined sources5
Turni205 – 207Combined sources3
Helixi211 – 226Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2Q3HX-ray1.73A32-230[»]
ProteinModelPortaliQ7L0Q8.
SMRiQ7L0Q8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7L0Q8.

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi26 – 61Gly-richSequence analysisAdd BLAST36

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rho family.1 Publication

Phylogenomic databases

eggNOGiKOG0393. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00760000118978.
HOGENOMiHOG000233974.
HOVERGENiHBG009351.
InParanoidiQ7L0Q8.
KOiK07865.
OMAiKKSKCRT.
OrthoDBiEOG091G0E4F.
PhylomeDBiQ7L0Q8.
TreeFamiTF321839.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51420. RHO. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 17 Publications (identifier: Q7L0Q8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPPQQGDPAF PDRCEAPPVP PRRERGGRGG RGPGEPGGRG RAGGAEGRGV
60 70 80 90 100
KCVLVGDGAV GKTSLVVSYT TNGYPTEYIP TAFDNFSAVV SVDGRPVRLQ
110 120 130 140 150
LCDTAGQDEF DKLRPLCYTN TDIFLLCFSV VSPSSFQNVS EKWVPEIRCH
160 170 180 190 200
CPKAPIILVG TQSDLREDVK VLIELDKCKE KPVPEEAAKL CAEEIKAASY
210 220 230 240 250
IECSALTQKN LKEVFDAAIV AGIQYSDTQQ QPKKSKSRTP DKMKNLSKSW

WKKYCCFV
Length:258
Mass (Da):28,218
Last modified:February 5, 2008 - v1
Checksum:iD90059DE82288B97
GO
Isoform 2 (identifier: Q7L0Q8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-61: Missing.
     62-66: KTSLV → MTNIG

Note: No experimental confirmation available.Curated
Show »
Length:197
Mass (Da):22,164
Checksum:iCAE1A9715F8B7D0B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1 – 13MPPQQ…AFPDR → QLLPTATLRGGGAVGPGPAS PRPQA in BAD92748 (Ref. 7) CuratedAdd BLAST13
Sequence conflicti71T → P in AAL99390 (PubMed:11894124).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_051975121T → A.Corresponds to variant rs3820264dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0527321 – 61Missing in isoform 2. 1 PublicationAdd BLAST61
Alternative sequenceiVSP_05273362 – 66KTSLV → MTNIG in isoform 2. 1 Publication5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF378087 mRNA. Translation: AAK83340.1.
AB074878 mRNA. Translation: BAB86361.1.
AB051826 mRNA. Translation: BAB18638.1.
DQ384420 Genomic DNA. Translation: ABD48870.1.
DQ384421 Genomic DNA. Translation: ABD48871.1.
DQ384422 Genomic DNA. Translation: ABD48872.1.
DQ384423 Genomic DNA. Translation: ABD48873.1.
DQ384424 Genomic DNA. Translation: ABD48874.1.
DQ384425 Genomic DNA. Translation: ABD48875.1.
AF211836 mRNA. Translation: AAL54874.1.
AF282258 mRNA. Translation: AAG46058.1.
AF251701 mRNA. Translation: AAL99390.1.
AK289971 mRNA. Translation: BAF82660.1.
AB209511 mRNA. Translation: BAD92748.1.
AL096776 Genomic DNA. Translation: CAC00584.1.
CH471098 Genomic DNA. Translation: EAW69885.1.
BC040076 mRNA. Translation: AAH40076.1.
CCDSiCCDS1575.1. [Q7L0Q8-1]
RefSeqiNP_067028.1. NM_021205.5. [Q7L0Q8-1]
UniGeneiHs.647774.

Genome annotation databases

EnsembliENST00000366691; ENSP00000355652; ENSG00000116574. [Q7L0Q8-1]
GeneIDi58480.
KEGGihsa:58480.
UCSCiuc001htf.3. human. [Q7L0Q8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF378087 mRNA. Translation: AAK83340.1.
AB074878 mRNA. Translation: BAB86361.1.
AB051826 mRNA. Translation: BAB18638.1.
DQ384420 Genomic DNA. Translation: ABD48870.1.
DQ384421 Genomic DNA. Translation: ABD48871.1.
DQ384422 Genomic DNA. Translation: ABD48872.1.
DQ384423 Genomic DNA. Translation: ABD48873.1.
DQ384424 Genomic DNA. Translation: ABD48874.1.
DQ384425 Genomic DNA. Translation: ABD48875.1.
AF211836 mRNA. Translation: AAL54874.1.
AF282258 mRNA. Translation: AAG46058.1.
AF251701 mRNA. Translation: AAL99390.1.
AK289971 mRNA. Translation: BAF82660.1.
AB209511 mRNA. Translation: BAD92748.1.
AL096776 Genomic DNA. Translation: CAC00584.1.
CH471098 Genomic DNA. Translation: EAW69885.1.
BC040076 mRNA. Translation: AAH40076.1.
CCDSiCCDS1575.1. [Q7L0Q8-1]
RefSeqiNP_067028.1. NM_021205.5. [Q7L0Q8-1]
UniGeneiHs.647774.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2Q3HX-ray1.73A32-230[»]
ProteinModelPortaliQ7L0Q8.
SMRiQ7L0Q8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121812. 127 interactors.
IntActiQ7L0Q8. 4 interactors.
MINTiMINT-7136717.
STRINGi9606.ENSP00000355652.

PTM databases

iPTMnetiQ7L0Q8.
PhosphoSitePlusiQ7L0Q8.
SwissPalmiQ7L0Q8.

Polymorphism and mutation databases

BioMutaiRHOU.
DMDMi172046189.

Proteomic databases

EPDiQ7L0Q8.
PaxDbiQ7L0Q8.
PeptideAtlasiQ7L0Q8.
PRIDEiQ7L0Q8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000366691; ENSP00000355652; ENSG00000116574. [Q7L0Q8-1]
GeneIDi58480.
KEGGihsa:58480.
UCSCiuc001htf.3. human. [Q7L0Q8-1]

Organism-specific databases

CTDi58480.
DisGeNETi58480.
GeneCardsiRHOU.
HGNCiHGNC:17794. RHOU.
HPAiHPA049592.
MIMi606366. gene.
neXtProtiNX_Q7L0Q8.
OpenTargetsiENSG00000116574.
PharmGKBiPA38246.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0393. Eukaryota.
COG1100. LUCA.
GeneTreeiENSGT00760000118978.
HOGENOMiHOG000233974.
HOVERGENiHBG009351.
InParanoidiQ7L0Q8.
KOiK07865.
OMAiKKSKCRT.
OrthoDBiEOG091G0E4F.
PhylomeDBiQ7L0Q8.
TreeFamiTF321839.

Enzyme and pathway databases

ReactomeiR-HSA-194840. Rho GTPase cycle.
SignaLinkiQ7L0Q8.

Miscellaneous databases

ChiTaRSiRHOU. human.
EvolutionaryTraceiQ7L0Q8.
GenomeRNAii58480.
PROiQ7L0Q8.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000116574.
CleanExiHS_RHOU.
ExpressionAtlasiQ7L0Q8. baseline and differential.
GenevisibleiQ7L0Q8. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51420. RHO. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRHOU_HUMAN
AccessioniPrimary (citable) accession number: Q7L0Q8
Secondary accession number(s): B1AKN1, Q59FE9, Q8TDQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: February 5, 2008
Last modified: November 2, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.