Q7L0J3 (SV2A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Synaptic vesicle glycoprotein 2A

Gene names

Name:	SV2A
Synonyms:	KIAA0736
ORF Names:	PSEC0174

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	742 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in the control of regulated secretion in neural and endocrine cells, enhancing selectively low-frequency neurotransmission. Positively regulates vesicle fusion by maintaining the readily releasable pool of secretory vesicles By similarity.
Subunit structure	Interacts with SYT1/synaptotagmin-1 in a calcium-dependent manner. Binds the adapter protein complex AP-2 By similarity.
Subcellular location	Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane; Multi-pass membrane protein By similarity. Note: Enriched in chromaffin granules, not present in adrenal microsomes. Associated with both insulin granules and synaptic-like microvesicles in insulin-secreting cells of the pancreas By similarity.
Post-translational modification	Phosphorylation by CK1 of the N-terminal cytoplasmic domain regulates interaction with SYT1 By similarity. N-glycosylated By similarity.
Miscellaneous	Identified as the brain binding-site for the antiepileptic drug levetiracetam/lev.
Sequence similarities	Belongs to the major facilitator superfamily.
Sequence caution	The sequence BAA34456.2 differs from that shown. Reason: Erroneous initiation. The sequence CAI12573.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Neurotransmitter transport Transport
Cellular component	Cell junction Cytoplasmic vesicle Membrane Synapse
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular calcium ion homeostasis Inferred from electronic annotation. Source: Compara neurotransmitter transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cell-cell junction Inferred from electronic annotation. Source: Compara endoplasmic reticulum Inferred from direct assay. Source: LIFEdb integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW neuromuscular junction Inferred from electronic annotation. Source: Compara neuron projection Inferred from electronic annotation. Source: Compara presynaptic active zone Inferred from electronic annotation. Source: Compara synaptic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	receptor activity Inferred from electronic annotation. Source: Compara transmembrane transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q7L0J3-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q7L0J3-2)

The sequence of this isoform differs from the canonical sequence as follows:
     683-742: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 742	742	Synaptic vesicle glycoprotein 2A		PRO_0000239764
Regions
	Topological domain	1 – 169	169	Cytoplasmic Potential
	Transmembrane	170 – 190	21	Helical; Potential
	Topological domain	191 – 205	15	Extracellular Potential
	Transmembrane	206 – 226	21	Helical; Potential
	Topological domain	227 – 233	7	Cytoplasmic Potential
	Transmembrane	234 – 254	21	Helical; Potential
	Topological domain	255 – 262	8	Extracellular Potential
	Transmembrane	263 – 283	21	Helical; Potential
	Topological domain	284 – 294	11	Cytoplasmic Potential
	Transmembrane	295 – 315	21	Helical; Potential
	Topological domain	316 – 334	19	Extracellular Potential
	Transmembrane	335 – 355	21	Helical; Potential
	Topological domain	356 – 447	92	Cytoplasmic Potential
	Transmembrane	448 – 468	21	Helical; Potential
	Topological domain	469 – 598	130	Extracellular Potential
	Transmembrane	599 – 619	21	Helical; Potential
	Topological domain	620 – 626	7	Cytoplasmic Potential
	Transmembrane	627 – 647	21	Helical; Potential
	Topological domain	648 – 651	4	Extracellular Potential
	Transmembrane	652 – 672	21	Helical; Potential
	Topological domain	673 – 685	13	Cytoplasmic Potential
	Transmembrane	686 – 708	23	Helical; Potential
	Topological domain	709 – 712	4	Extracellular Potential
	Transmembrane	713 – 731	19	Helical; Potential
	Topological domain	732 – 742	11	Cytoplasmic Potential
	Region	1 – 57	57	Interaction with SYT1 By similarity
Amino acid modifications
	Modified residue	65	1	Phosphotyrosine By similarity
	Modified residue	66	1	Phosphotyrosine By similarity
	Modified residue	80	1	Phosphoserine Ref.9
	Modified residue	81	1	Phosphoserine Ref.9
	Modified residue	84	1	Phosphothreonine Ref.9
	Modified residue	127	1	Phosphoserine By similarity
	Modified residue	480	1	Phosphotyrosine By similarity
	Glycosylation	498	1	N-linked (GlcNAc...) Potential
	Glycosylation	548	1	N-linked (GlcNAc...) Potential
	Glycosylation	573	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	683 – 742	60	Missing in isoform 2.		VSP_019265
Experimental info
	Sequence conflict	100	1	G → D in CAD97824. Ref.4
	Sequence conflict	404	1	D → G in BAC11645. Ref.3
	Sequence conflict	493	1	E → G in CAD97824. Ref.4
	Sequence conflict	524	1	V → A in BAC11645. Ref.3
	Sequence conflict	544	1	T → A in BAC11645. Ref.3
	Sequence conflict	582	1	G → D in BAC11645. Ref.3
	Sequence conflict	611	1	G → R in BAC11645. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 913E216D5CFC2FB2	FASTA	742	82,695
	10 20 30 40 50 60 MEEGFRDRAA FIRGAKDIAK EVKKHAAKKV VKGLDRVQDE YSRRSYSRFE EEDDDDDFPA 70 80 90 100 110 120 PSDGYYRGEG TQDEEEGGAS SDATEGHDED DEIYEGEYQG IPRAESGGKG ERMADGAPLA 130 140 150 160 170 180 GVRGGLSDGE GPPGGRGEAQ RRKEREELAQ QYEAILRECG HGRFQWTLYF VLGLALMADG 190 200 210 220 230 240 VEVFVVGFVL PSAEKDMCLS DSNKGMLGLI VYLGMMVGAF LWGGLADRLG RRQCLLISLS 250 260 270 280 290 300 VNSVFAFFSS FVQGYGTFLF CRLLSGVGIG GSIPIVFSYF SEFLAQEKRG EHLSWLCMFW 310 320 330 340 350 360 MIGGVYAAAM AWAIIPHYGW SFQMGSAYQF HSWRVFVLVC AFPSVFAIGA LTTQPESPRF 370 380 390 400 410 420 FLENGKHDEA WMVLKQVHDT NMRAKGHPER VFSVTHIKTI HQEDELIEIQ SDTGTWYQRW 430 440 450 460 470 480 GVRALSLGGQ VWGNFLSCFG PEYRRITLMM MGVWFTMSFS YYGLTVWFPD MIRHLQAVDY 490 500 510 520 530 540 ASRTKVFPGE RVEHVTFNFT LENQIHRGGQ YFNDKFIGLR LKSVSFEDSL FEECYFEDVT 550 560 570 580 590 600 SSNTFFRNCT FINTVFYNTD LFEYKFVNSR LINSTFLHNK EGCPLDVTGT GEGAYMVYFV 610 620 630 640 650 660 SFLGTLAVLP GNIVSALLMD KIGRLRMLAG SSVMSCVSCF FLSFGNSESA MIALLCLFGG 670 680 690 700 710 720 VSIASWNALD VLTVELYPSD KRTTAFGFLN ALCKLAAVLG ISIFTSFVGI TKAAPILFAS 730 740 AALALGSSLA LKLPETRGQV LQ
	Isoform 2 [UniParc]. Checksum: 926FD8E2F5EAF88F	FASTA	682	76,619
	10 20 30 40 50 60 MEEGFRDRAA FIRGAKDIAK EVKKHAAKKV VKGLDRVQDE YSRRSYSRFE EEDDDDDFPA 70 80 90 100 110 120 PSDGYYRGEG TQDEEEGGAS SDATEGHDED DEIYEGEYQG IPRAESGGKG ERMADGAPLA 130 140 150 160 170 180 GVRGGLSDGE GPPGGRGEAQ RRKEREELAQ QYEAILRECG HGRFQWTLYF VLGLALMADG 190 200 210 220 230 240 VEVFVVGFVL PSAEKDMCLS DSNKGMLGLI VYLGMMVGAF LWGGLADRLG RRQCLLISLS 250 260 270 280 290 300 VNSVFAFFSS FVQGYGTFLF CRLLSGVGIG GSIPIVFSYF SEFLAQEKRG EHLSWLCMFW 310 320 330 340 350 360 MIGGVYAAAM AWAIIPHYGW SFQMGSAYQF HSWRVFVLVC AFPSVFAIGA LTTQPESPRF 370 380 390 400 410 420 FLENGKHDEA WMVLKQVHDT NMRAKGHPER VFSVTHIKTI HQEDELIEIQ SDTGTWYQRW 430 440 450 460 470 480 GVRALSLGGQ VWGNFLSCFG PEYRRITLMM MGVWFTMSFS YYGLTVWFPD MIRHLQAVDY 490 500 510 520 530 540 ASRTKVFPGE RVEHVTFNFT LENQIHRGGQ YFNDKFIGLR LKSVSFEDSL FEECYFEDVT 550 560 570 580 590 600 SSNTFFRNCT FINTVFYNTD LFEYKFVNSR LINSTFLHNK EGCPLDVTGT GEGAYMVYFV 610 620 630 640 650 660 SFLGTLAVLP GNIVSALLMD KIGRLRMLAG SSVMSCVSCF FLSFGNSESA MIALLCLFGG 670 680 VSIASWNALD VLTVELYPSD KR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Ishikawa K., Suyama M., Kikuno R., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O. DNA Res. 5:277-286(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[2]	Ohara O., Suyama M., Nagase T., Ishikawa K., Kikuno R. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries." Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y. , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T. DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Teratocarcinoma.
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Retina.
[5]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain and Ovary.
[8]	"The synaptic vesicle protein SV2A is the binding site for the antiepileptic drug levetiracetam." Lynch B.A., Lambeng N., Nocka K., Kensel-Hammes P., Bajjalieh S.M., Matagne A., Fuks B. Proc. Natl. Acad. Sci. U.S.A. 101:9861-9866(2004) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION AS BINDING-SITE FOR ANTIEPILEPTIC DRUG LEVETIRACETAM.
[9]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80; SER-81 AND THR-84, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB018279 mRNA. Translation: BAA34456.2. Different initiation. AK075480 mRNA. Translation: BAC11645.1. BX537754 mRNA. Translation: CAD97824.1. AL591493 Genomic DNA. Translation: CAI12572.1. AL591493 Genomic DNA. Translation: CAI12573.1. Sequence problems. CH471121 Genomic DNA. Translation: EAW53596.1. CH471121 Genomic DNA. Translation: EAW53598.1. BC000776 mRNA. Translation: AAH00776.2. BC045111 mRNA. Translation: AAH45111.1.
IPI	IPI00644025. IPI00645484.
RefSeq	NP_055664.3. NM_014849.3.
UniGene	Hs.516153.
3D structure databases
ProteinModelPortal	Q7L0J3.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q7L0J3. 3 interactions.
STRING	9606.ENSP00000358142.
PTM databases
PhosphoSite	Q7L0J3.
Polymorphism databases
DMDM	74749878.
Proteomic databases
PaxDb	Q7L0J3.
PeptideAtlas	Q7L0J3.
PRIDE	Q7L0J3.
Protocols and materials databases
DNASU	9900.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000369145; ENSP00000358141; ENSG00000159164. ENST00000369146; ENSP00000358142; ENSG00000159164. ENST00000581609; ENSP00000463016; ENSG00000265307. ENST00000584087; ENSP00000462467; ENSG00000265307.
GeneID	9900.
KEGG	hsa:9900.
UCSC	uc001etg.3. human.
Organism-specific databases
CTD	9900.
GeneCards	GC01M149874.
HGNC	HGNC:20566. SV2A.
HPA	CAB002226. HPA007863.
MIM	185860. gene.
neXtProt	NX_Q7L0J3.
PharmGKB	PA128394564.
HUGE	Search...
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0477.
HOVERGEN	HBG053967.
InParanoid	Q7L0J3.
KO	K06258.
OMA	LTTMPES.
OrthoDB	EOG4K0QMZ.
PhylomeDB	Q7L0J3.
Gene expression databases
Bgee	Q7L0J3.
CleanEx	HS_SV2A.
Genevestigator	Q7L0J3.
GermOnline	ENSG00000159164. Homo sapiens.
Family and domain databases
InterPro	IPR001646. 5peptide_repeat. IPR011701. MFS. IPR020846. MFS_dom. IPR016196. MFS_dom_general_subst_transpt. IPR005828. Sub_transporter. IPR005829. Sugar_transporter_CS. IPR022308. SV2. [Graphical view]
PANTHER	PTHR24065. PTHR24065. 1 hit.
Pfam	PF07690. MFS_1. 1 hit. PF13599. Pentapeptide_4. 1 hit. PF00083. Sugar_tr. 1 hit. [Graphical view]
SUPFAM	SSF103473. MFS_gen_substrate_transporter. 2 hits.
TIGRFAMs	TIGR01299. synapt_SV2. 1 hit.
PROSITE	PS50850. MFS. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL1998.
ChiTaRS	SV2A. human.
DrugBank	DB01202. Levetiracetam.
GenomeRNAi	9900.
NextBio	37327.
SOURCE	Search...

Entry information

Entry name

SV2A_HUMAN

Accession

Primary (citable) accession number: Q7L0J3
Secondary accession number(s): D3DUZ7 , O94841, Q5QNX8, Q7Z3L6, Q8NBJ6, Q9BVZ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 13, 2006
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.