Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable ATP-dependent RNA helicase DDX46

Gene

DDX46

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an essential role in splicing, either prior to, or during splicing A complex formation.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi416 – 4238ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: GO_Central
  • poly(A) RNA binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent RNA helicase DDX46 (EC:3.6.4.13)
Alternative name(s):
DEAD box protein 46
PRP5 homolog
Gene namesi
Name:DDX46
Synonyms:KIAA0801
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:18681. DDX46.

Subcellular locationi

GO - Cellular componenti

  • Cajal body Source: UniProtKB-SubCell
  • membrane Source: UniProtKB-SubCell
  • nuclear speck Source: UniProtKB-SubCell
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134894452.

Polymorphism and mutation databases

BioMutaiDDX46.
DMDMi116241326.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 10311030Probable ATP-dependent RNA helicase DDX46PRO_0000055121Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine1 Publication
Cross-linki186 – 186Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei199 – 1991PhosphoserineBy similarity
Modified residuei263 – 2631N6-acetyllysineCombined sources
Modified residuei294 – 2941PhosphotyrosineCombined sources
Modified residuei295 – 2951PhosphoserineCombined sources
Modified residuei296 – 2961PhosphoserineCombined sources
Modified residuei346 – 3461PhosphoserineCombined sources
Modified residuei776 – 7761N6-acetyllysineCombined sources
Modified residuei804 – 8041PhosphoserineCombined sources
Modified residuei903 – 9031N6-acetyllysineBy similarity
Modified residuei928 – 9281PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Isopeptide bond, Lipoprotein, Myristate, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ7L014.
MaxQBiQ7L014.
PaxDbiQ7L014.
PeptideAtlasiQ7L014.
PRIDEiQ7L014.

PTM databases

iPTMnetiQ7L014.
PhosphoSiteiQ7L014.
SwissPalmiQ7L014.

Miscellaneous databases

PMAP-CutDBQ7L014.

Expressioni

Gene expression databases

BgeeiENSG00000145833.
CleanExiHS_DDX46.
ExpressionAtlasiQ7L014. baseline and differential.
GenevisibleiQ7L014. HS.

Organism-specific databases

HPAiHPA036554.
HPA057501.

Interactioni

Subunit structurei

Integral component of the 17S U2 snRNP.1 Publication

Protein-protein interaction databases

BioGridi115210. 44 interactions.
IntActiQ7L014. 9 interactions.
MINTiMINT-3002486.
STRINGi9606.ENSP00000346236.

Structurei

3D structure databases

ProteinModelPortaliQ7L014.
SMRiQ7L014. Positions 327-738.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini403 – 581179Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini592 – 753162Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili152 – 19746Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi372 – 40029Q motifAdd
BLAST
Motifi529 – 5324DEAD box

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi3 – 103101Arg-richAdd
BLAST
Compositional biasi202 – 2098Poly-Asp

Sequence similaritiesi

Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0334. Eukaryota.
ENOG410XSQV. LUCA.
GeneTreeiENSGT00840000129934.
HOGENOMiHOG000007229.
HOVERGENiHBG081426.
InParanoidiQ7L014.
KOiK12811.
PhylomeDBiQ7L014.
TreeFamiTF354236.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7L014-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRESRHYRK RSASRGRSGS RSRSRSPSDK RSKRGDDRRS RSRDRDRRRE
60 70 80 90 100
RSRSRDKRRS RSRDRKRLRR SRSRERDRSR ERRRSRSRDR RRSRSRSRGR
110 120 130 140 150
RSRSSSPGNK SKKTENRSRS KEKTDGGESS KEKKKDKDDK EDEKEKDAGN
160 170 180 190 200
FDQNKLEEEM RKRKERVEKW REEQRKKAME NIGELKKEIE EMKQGKKWSL
210 220 230 240 250
EDDDDDEDDP AEAEKEGNEM EGEELDPLDA YMEEVKEEVK KFNMRSVKGG
260 270 280 290 300
GGNEKKSGPT VTKVVTVVTT KKAVVDSDKK KGELMENDQD AMEYSSEEEE
310 320 330 340 350
VDLQTALTGY QTKQRKLLEP VDHGKIEYEP FRKNFYVEVP ELAKMSQEEV
360 370 380 390 400
NVFRLEMEGI TVKGKGCPKP IKSWVQCGIS MKILNSLKKH GYEKPTPIQT
410 420 430 440 450
QAIPAIMSGR DLIGIAKTGS GKTIAFLLPM FRHIMDQRSL EEGEGPIAVI
460 470 480 490 500
MTPTRELALQ ITKECKKFSK TLGLRVVCVY GGTGISEQIA ELKRGAEIIV
510 520 530 540 550
CTPGRMIDML AANSGRVTNL RRVTYVVLDE ADRMFDMGFE PQVMRIVDNV
560 570 580 590 600
RPDRQTVMFS ATFPRAMEAL ARRILSKPIE VQVGGRSVVC SDVEQQVIVI
610 620 630 640 650
EEEKKFLKLL ELLGHYQESG SVIIFVDKQE HADGLLKDLM RASYPCMSLH
660 670 680 690 700
GGIDQYDRDS IINDFKNGTC KLLVATSVAA RGLDVKHLIL VVNYSCPNHY
710 720 730 740 750
EDYVHRAGRT GRAGNKGYAY TFITEDQARY AGDIIKALEL SGTAVPPDLE
760 770 780 790 800
KLWSDFKDQQ KAEGKIIKKS SGFSGKGFKF DETEQALANE RKKLQKAALG
810 820 830 840 850
LQDSDDEDAA VDIDEQIESM FNSKKRVKDM AAPGTSSVPA PTAGNAEKLE
860 870 880 890 900
IAKRLALRIN AQKNLGIESQ DVMQQATNAI LRGGTILAPT VSAKTIAEQL
910 920 930 940 950
AEKINAKLNY VPLEKQEEER QDGGQNESFK RYEEELEIND FPQTARWKVT
960 970 980 990 1000
SKEALQRISE YSEAAITIRG TYFPPGKEPK EGERKIYLAI ESANELAVQK
1010 1020 1030
AKAEITRLIK EELIRLQNSY QPTNKGRYKV L
Length:1,031
Mass (Da):117,362
Last modified:October 17, 2006 - v2
Checksum:i52535164FEB48A72
GO

Sequence cautioni

The sequence BAA34521 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti109 – 1091N → S in AAD43033 (Ref. 1) Curated
Sequence conflicti114 – 1141T → A in AAD43033 (Ref. 1) Curated
Sequence conflicti124 – 1252TD → AE in AAD43033 (Ref. 1) Curated
Sequence conflicti128 – 1281E → D in AAD43033 (Ref. 1) Curated
Sequence conflicti222 – 2221G → D in AAD43033 (Ref. 1) Curated
Sequence conflicti251 – 2511G → A in AAD43033 (Ref. 1) Curated
Sequence conflicti447 – 4471I → L in AAD43033 (Ref. 1) Curated
Sequence conflicti462 – 4698TKECKKFS → PKGVRSF in AAD43033 (Ref. 1) Curated
Sequence conflicti870 – 8701Q → QV in AAD43033 (Ref. 1) Curated
Sequence conflicti870 – 8701Q → QV in BAA34521 (PubMed:9872452).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti207 – 2071E → Q.
Corresponds to variant rs10447293 [ dbSNP | Ensembl ].
VAR_028079

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF106680 mRNA. Translation: AAD43033.1.
AB018344 mRNA. Translation: BAA34521.2. Different initiation.
BC012304 mRNA. Translation: AAH12304.1.
BK000565 mRNA. Translation: DAA00076.1.
CCDSiCCDS34240.1.
RefSeqiNP_001287789.1. NM_001300860.1.
NP_055644.2. NM_014829.3.
UniGeneiHs.406549.

Genome annotation databases

EnsembliENST00000354283; ENSP00000346236; ENSG00000145833.
GeneIDi9879.
KEGGihsa:9879.
UCSCiuc003kzw.5. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF106680 mRNA. Translation: AAD43033.1.
AB018344 mRNA. Translation: BAA34521.2. Different initiation.
BC012304 mRNA. Translation: AAH12304.1.
BK000565 mRNA. Translation: DAA00076.1.
CCDSiCCDS34240.1.
RefSeqiNP_001287789.1. NM_001300860.1.
NP_055644.2. NM_014829.3.
UniGeneiHs.406549.

3D structure databases

ProteinModelPortaliQ7L014.
SMRiQ7L014. Positions 327-738.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115210. 44 interactions.
IntActiQ7L014. 9 interactions.
MINTiMINT-3002486.
STRINGi9606.ENSP00000346236.

PTM databases

iPTMnetiQ7L014.
PhosphoSiteiQ7L014.
SwissPalmiQ7L014.

Polymorphism and mutation databases

BioMutaiDDX46.
DMDMi116241326.

Proteomic databases

EPDiQ7L014.
MaxQBiQ7L014.
PaxDbiQ7L014.
PeptideAtlasiQ7L014.
PRIDEiQ7L014.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000354283; ENSP00000346236; ENSG00000145833.
GeneIDi9879.
KEGGihsa:9879.
UCSCiuc003kzw.5. human.

Organism-specific databases

CTDi9879.
GeneCardsiDDX46.
HGNCiHGNC:18681. DDX46.
HPAiHPA036554.
HPA057501.
neXtProtiNX_Q7L014.
PharmGKBiPA134894452.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0334. Eukaryota.
ENOG410XSQV. LUCA.
GeneTreeiENSGT00840000129934.
HOGENOMiHOG000007229.
HOVERGENiHBG081426.
InParanoidiQ7L014.
KOiK12811.
PhylomeDBiQ7L014.
TreeFamiTF354236.

Miscellaneous databases

ChiTaRSiDDX46. human.
GeneWikiiDDX46.
GenomeRNAii9879.
PMAP-CutDBQ7L014.
PROiQ7L014.

Gene expression databases

BgeeiENSG00000145833.
CleanExiHS_DDX46.
ExpressionAtlasiQ7L014. baseline and differential.
GenevisibleiQ7L014. HS.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDDX46_HUMAN
AccessioniPrimary (citable) accession number: Q7L014
Secondary accession number(s): O94894, Q96EI0, Q9Y658
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: October 17, 2006
Last modified: September 7, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.