Q7KWQ2 (SYSC_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine--tRNA ligase, cytoplasmic
EC=6.1.1.11

Alternative name(s):
Seryl-tRNA synthetase
Short name=SerRS
Seryl-tRNA(Ser/Sec) synthetase

Gene names

Name:	serS
ORF Names:	DDB_G0272660

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	451 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec) By similarity.
Catalytic activity	ATP + L-serine + tRNA(Ser) = AMP + diphosphate + L-seryl-tRNA(Ser). ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec).
Pathway	Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1.
Subunit structure	Homodimer. The tRNA molecule binds across the dimer By similarity.
Subcellular location	Cytoplasm.
Domain	Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	seryl-tRNA aminoacylation Inferred from sequence or structural similarity. Source: dictyBase
Cellular component	phagocytic vesicle Inferred from direct assay Ref.3. Source: dictyBase
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW serine-tRNA ligase activity Inferred from sequence or structural similarity. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 451	451	Serine--tRNA ligase, cytoplasmic		PRO_0000328594
Regions
	Nucleotide binding	267 – 269	3	ATP By similarity
	Nucleotide binding	354 – 357	4	ATP By similarity
	Region	236 – 238	3	Serine binding By similarity
Sites
	Binding site	283	1	ATP; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	290	1	Serine By similarity
	Binding site	392	1	Serine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7KWQ2 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: E3F1DA42D4A13FF4	FASTA	451	51,578
	10 20 30 40 50 60 MGLDIVLFRA DKGGNPDLIR QSQKVRYANV DAVQEVIDLD KVVKETKYRL DNTNAEYAKL 70 80 90 100 110 120 NKSVAMKKKA GESADEIIAQ AEELNQSIIK LKAEVSEVEL LLRKKLRPIG NIVHESVPID 130 140 150 160 170 180 NNEDNNQIIK TWGECKTSEG LLHHHELLEM IDGYDPERGT LVSGHRCYFL KGIGVLLNQA 190 200 210 220 230 240 IINFALMHMT KRGSVPLQTP FFMNKDVMAK TAQLEQFDDE LYKVTGDNEE KYLIATSEQP 250 260 270 280 290 300 ISAFHQDEWI EEKDLPKKYV GYSTCFRKEA GSHGRDTWGI FRVHQFEKIE QFCITEPEKS 310 320 330 340 350 360 WDMMEEMINN SEQFYQELGI PYRVVNIVSG ALNNAASKKY DLEGWFPGYN QYRELVSCSN 370 380 390 400 410 420 CTDYQSRDLE IRCGMKKQGQ QQKKYVHMLN STLAATTRVI CCILENYQTE GGITVPVPLR 430 440 450 PYLGKDFIPF VKAAPKQKAI PKQKTTTPEQ K

References

[1]	"Sequence and analysis of chromosome 2 of Dictyostelium discoideum." Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A. Nature 418:79-85(2002) [PubMed: 12097910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake." Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T. Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed: 16926386] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Strain: AX2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000008 Genomic DNA. Translation: EAL70967.1.
RefSeq	XP_644993.1. XM_639901.1.
3D structure databases
HSSP	HSSP built from PDB template 1WLE based on UniProtKB Q9N0F3.
ProteinModelPortal	Q7KWQ2.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7KWQ2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0231305; DDB0231305; DDB_G0272660.
GeneID	8618670.
GenomeReviews	Gene locus serS in contig CM000151_GR.
KEGG	ddi:DDB_G0272660.
Organism-specific databases
dictyBase	DDB_G0272660. serS.
Phylogenomic databases
eggNOG	KOG2509.
GeneTree	EPrGT00050000002073.
HOGENOM	HBG629391.
OMA	MMDEMIG.
PhylomeDB	Q7KWQ2.
ProtClustDB	CLSZ2496096.
Family and domain databases
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR002317. Ser-tRNA-synth_IIa. IPR015866. Ser-tRNA-synth_IIa_N. IPR010978. tRNA-bd_arm. [Graphical view]
Gene3D	G3DSA:1.10.287.40. Ser-tRNA-synth_IIa_N. 1 hit.
KO	K01875.
PANTHER	PTHR11778. tRNA-synt_ser. 1 hit.
Pfam	PF02403. Seryl_tRNA_N. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PIRSF	PIRSF001529. Ser-tRNA-synth_IIa. 1 hit.
PRINTS	PR00981. TRNASYNTHSER.
SUPFAM	SSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMs	TIGR00414. SerS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYSC_DICDI

Accession

Primary (citable) accession number: Q7KWQ2
Secondary accession number(s): Q558S3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)