Reviewed, UniProtKB/Swiss-Prot Q7KW39 (MMSA_DROME)

Names and origin

Protein names

Recommended name:
    Probable methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial
      Short name=MMSDH
      Short name=Malonate-semialdehyde dehydrogenase [acylating]
    EC=1.2.1.27
    EC=1.2.1.18

Gene names

ORF Names:

CG17896

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	520 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Plays a role in valine and pyrimidine metabolism. Binds fatty acyl-CoA By similarity. UniProtKB Q02252
Catalytic activity	2-methyl-3-oxopropanoate + CoA + H2O + NAD+ = propanoyl-CoA + HCO3- + NADH. 3-oxopropanoate + CoA + NAD(P)+ = acetyl-CoA + CO2 + NAD(P)H. UniProtKB Q02252
Subunit structure	Homotetramer By similarity. UniProtKB Q02252
Subcellular location	Mitochondrion By similarity. UniProtKB Q02252
Sequence similarities	Belongs to the aldehyde dehydrogenase family.
Sequence caution	The sequence CAA15632.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Mitochondrion
Coding sequence diversity	Alternative splicing
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW thymine metabolic process Inferred from sequence or structural similarity. Source: UniProtKB valine metabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acyl-CoA binding Inferred from sequence or structural similarity. Source: UniProtKB malonate-semialdehyde dehydrogenase (acetylating) activity Inferred from sequence or structural similarity. Source: UniProtKB methylmalonate-semialdehyde dehydrogenase (acylating) activity Inferred from sequence or structural similarity. Source: UniProtKB protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Kap-alpha1	O76521	1	EBI-98272,EBI-189291

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform B Ref.1 (identifier: Q7KW39-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform A Ref.1 (identifier: Q7KW39-2)

The sequence of this isoform differs from the canonical sequence as follows:
     2-10: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 520		Probable methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial		PRO_0000043373
Regions
	Nucleotide binding	195 – 199	5	NAD Potential
	Nucleotide binding	247 – 252	6	NAD Potential
Sites
	Active site	303	1	Nucleophile By similarity UniProtKB Q02252
	Binding site	403	1	NAD Potential
Natural variations
	Alternative sequence	2 – 10	9	Missing in isoform A.		VSP_051919

Sequences

	Sequence		Length	Mass (Da)
	Isoform B [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 12AC03D9B9C3E41D	FASTA	520	55,972
	10 20 30 40 50 60 MSLVRLIGAE ARHLAKRSYS SAAPTTKLFI DGKFVESKTN EWIDVHDPAT NQVVTRVPKA 70 80 90 100 110 120 TQAEMQAALE SNKKAFRSWS NQSILTRQQV MFKLQALIKE NMGELAKNIT KEQGKTLADA 130 140 150 160 170 180 EGDVLRGLQV VEHCCSIPSL QMGETVANVA RDMDTYSLVL PLGVTAGVAP FNFPAMIPLW 190 200 210 220 230 240 MFPVAITTGN TMLLKPSERV PGATMLLMEL LNEAGCPPGV VNVIHGQHDA VNFICDAPEI 250 260 270 280 290 300 KAVSFVGSDQ AGKYIYERAG KNGKRVQSNM GAKNHGIILG DANKENTLNQ LAGAAFGAAG 310 320 330 340 350 360 QRCMALSTAV FVGDAQAWIP DLVERAQKLK VNAGHVPGTD VGPVISAASR QRINDLIESG 370 380 390 400 410 420 VKEGAKLILD GRKITVPGYE DGYFVGPTIL SDVTPSMKCY TEEIFGPVLV ILKADTLDDA 430 440 450 460 470 480 IGIVNANPYG NGTAVFTTNG AAARKFVNEI DAGQVGVNVP IPVPLPMFSF TGTRGSFRGD 490 500 510 520 HHFYGKQGIK FYTQTKTVTQ LWRKTDVTHT QAAVAMPTMK
	Isoform A. Checksum: 304898AEFE153447	FASTA	511	55,033
	10 20 30 40 50 60 MARHLAKRSY SSAAPTTKLF IDGKFVESKT NEWIDVHDPA TNQVVTRVPK ATQAEMQAAL 70 80 90 100 110 120 ESNKKAFRSW SNQSILTRQQ VMFKLQALIK ENMGELAKNI TKEQGKTLAD AEGDVLRGLQ 130 140 150 160 170 180 VVEHCCSIPS LQMGETVANV ARDMDTYSLV LPLGVTAGVA PFNFPAMIPL WMFPVAITTG 190 200 210 220 230 240 NTMLLKPSER VPGATMLLME LLNEAGCPPG VVNVIHGQHD AVNFICDAPE IKAVSFVGSD 250 260 270 280 290 300 QAGKYIYERA GKNGKRVQSN MGAKNHGIIL GDANKENTLN QLAGAAFGAA GQRCMALSTA 310 320 330 340 350 360 VFVGDAQAWI PDLVERAQKL KVNAGHVPGT DVGPVISAAS RQRINDLIES GVKEGAKLIL 370 380 390 400 410 420 DGRKITVPGY EDGYFVGPTI LSDVTPSMKC YTEEIFGPVL VILKADTLDD AIGIVNANPY 430 440 450 460 470 480 GNGTAVFTTN GAAARKFVNE IDAGQVGVNV PIPVPLPMFS FTGTRGSFRG DHHFYGKQGI 490 500 510 KFYTQTKTVT QLWRKTDVTH TQAAVAMPTM K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
[3]	"From sequence to chromosome: the tip of the X chromosome of D. melanogaster." Benos P.V., Gatt M.K., Ashburner M., Murphy L., Harris D., Barrell B.G., Ferraz C., Vidal S., Brun C., Demailles J., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Borkova D., Minana B. , Kafatos F.C., Louis C., Siden-Kiamos I., Bolshakov S., Papagiannakis G., Spanos L., Cox S., Madueno E., de Pablos B., Modolell J., Peter A., Schoettler P., Werner M., Mourkioti F., Beinert N., Dowe G., Schaefer U., Jaeckle H., Bucheton A., Callister D.M., Campbell L.A., Darlamitsou A., Henderson N.S., McMillan P.J., Salles C., Tait E.A., Valenti P., Saunders R.D.C., Glover D.M. Science 287:2220-2222(2000) [PubMed: 10731137] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Oregon-R.
[4]	Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E. Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 332-520. Strain: Berkeley. Tissue: Ovary.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014298 Genomic DNA. Translation: AAF45510.2. AE014298 Genomic DNA. Translation: AAF45511.1. AL009147 Genomic DNA. Translation: CAA15632.1. Sequence problems. AL009147 Genomic DNA. Translation: CAB41309.1. AY069284 mRNA. Translation: AAL39429.2.
PIR	T13418.
RefSeq	NP_569845.2. NP_726672.1.
UniGene	Dm.11239
3D structure databases
HSSP	HSSP built from PDB template 1BXS based on UniProtKB P51977.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q7KW39. 2 interactions.
Proteomic databases
PRIDE	Q7KW39.
Genome annotation databases
Ensembl	FBgn0023537. Drosophila melanogaster. [Contig view]
GeneID	30995.
KEGG	dme:Dmel_CG17896.
Organism-specific databases
FlyBase	FBgn0023537. CG17896.
Phylogenomic databases
HOGENOM	Q7KW39.
OMA	Q7KW39. IISNVKP.
Enzyme and pathway databases
BRENDA	1.2.1.18. 48. 1.2.1.27. 48.
Gene expression databases
ArrayExpress	Q7KW39.
GermOnline	CG17896. Drosophila melanogaster.
Family and domain databases
InterPro	IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR010061. MeMal-semiAld_DH. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit. PTHR11699:SF27. MMSDH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01722. MMSDH. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	771368.

Entry information

Entry name

MMSA_DROME

Accession

Primary (citable) accession number: Q7KW39
Secondary accession number(s): O46056, Q8T9I0, Q9V408

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	July 5, 2004
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project