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Protein

Isocitrate dehydrogenase [NADP]

Gene

Idh

Organism
Drosophila melanogaster (Fruit fly)
Status
Unreviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 1 Mg2+ or Mn2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei77SubstrateUniRule annotation1
Binding sitei82NADPUniRule annotation1
Binding sitei109SubstrateUniRule annotation1
Binding sitei132SubstrateUniRule annotation1
Sitei139Critical for catalysisUniRule annotation1
Sitei210Critical for catalysisUniRule annotation1
Metal bindingi250Magnesium or manganeseUniRule annotation1
Binding sitei258NADPUniRule annotation1
Metal bindingi273Magnesium or manganeseUniRule annotation1
Binding sitei326NADP; via amide nitrogen and carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi75 – 77NADPUniRule annotation3
Nucleotide bindingi308 – 313NADPUniRule annotation6

GO - Molecular functioni

  • hydroxyacid-oxoacid transhydrogenase activity Source: FlyBase
  • isocitrate dehydrogenase (NADP+) activity Source: FlyBase
  • magnesium ion binding Source: InterPro
  • NAD binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionOxidoreductaseUniRule annotationImported
Biological processTricarboxylic acid cycleUniRule annotation
LigandMagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, NADPUniRule annotation

Enzyme and pathway databases

ReactomeiR-DME-389542. NADPH regeneration.
R-DME-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NADP]UniRule annotation (EC:1.1.1.42UniRule annotation)
Gene namesi
Name:IdhImported
Synonyms:cIdhImported, CT22171Imported, Dmel\CG7176Imported, ICDHImported, IDHImported, idhImported, IDH-NADPImported, Idh-NADPImported, isocitrate dehydrogenaseImported, l(3)L3852Imported
ORF Names:CG7176Imported, Dmel_CG7176Imported
OrganismiDrosophila melanogaster (Fruit fly)Imported
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0001248. Idh.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Expressioni

Gene expression databases

BgeeiFBgn0001248.

Interactioni

Protein-protein interaction databases

IntActiQ7KUB0. 3 interactors.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 400Iso_dhInterPro annotationAdd BLAST392

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni94 – 100Substrate bindingUniRule annotation7

Sequence similaritiesi

Belongs to the isocitrate and isopropylmalate dehydrogenases family.UniRule annotation

Phylogenomic databases

eggNOGiKOG1526. Eukaryota.
COG0538. LUCA.
GeneTreeiENSGT00390000012547.
OrthoDBiEOG091G06IY.

Family and domain databases

InterProiView protein in InterPro
IPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiView protein in Pfam
PF00180. Iso_dh. 1 hit.
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
SMARTiView protein in SMART
SM01329. Iso_dh. 1 hit.
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiView protein in PROSITE
PS00470. IDH_IMDH. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7KUB0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQKIKAGPV VDVLGDEMTR IIWDSIKSQL ILPFLDIELH TYDLGIENRD
60 70 80 90 100
KTEDQVTIDC AEAIKKYNVG IKCATITPDE KRVEEFNLKK MWKSPNGTIR
110 120 130 140 150
NILGGTVFRE AIICKNVPRL VTGWQKPIVI GRHAHADQYK AVDYVVPGPG
160 170 180 190 200
KLTLTWKGTD GQVIDEVIND FKGPGIALGM FNTDDSIVDF AHASFKYALD
210 220 230 240 250
RKLPLYMSTK NTILKKYDGR FKDIFEDLYN KQYKKEYEAA GIWYEHRLID
260 270 280 290 300
DMVAYAMKSE GGFVWACKNY DGDVQSDSVA QGYGSLGLMT SVLLCPDGKT
310 320 330 340 350
VEAEAAHGTV TRHFRFYQQG KETSTNPIAS IFAWTRGLLH RAKLDNNEPL
360 370 380 390 400
KQFADTLEQV CIDTIESGAM TKDLAICIKG NINAVTRRDY QETFEFINTL
410
AKNLEGALAK NAVAAK
Length:416
Mass (Da):46,660
Last modified:July 5, 2004 - v1
Checksum:iD610887825DC7CC4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA. Translation: AAF50435.1.
AE014296 Genomic DNA. Translation: AAN12004.1.
RefSeqiNP_729368.1. NM_168267.2.
NP_729370.1. NM_168269.2.
UniGeneiDm.4678.

Genome annotation databases

EnsemblMetazoaiFBtr0076667; FBpp0076390; FBgn0001248.
FBtr0076668; FBpp0076391; FBgn0001248.
FBtr0076670; FBpp0076393; FBgn0001248.
GeneIDi44291.
UCSCiCG7176-RA. d. melanogaster.

Similar proteinsi

Entry informationi

Entry nameiQ7KUB0_DROME
AccessioniPrimary (citable) accession number: Q7KUB0
Entry historyiIntegrated into UniProtKB/TrEMBL: July 5, 2004
Last sequence update: July 5, 2004
Last modified: September 27, 2017
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Proteomics identificationCombined sources, Reference proteomeImported