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Q7KT91

- C3390_DROME

UniProt

Q7KT91 - C3390_DROME

Protein

Non-lysosomal glucosylceramidase

Gene

CG33090

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Non-lysosomal glucosylceramidase that catalyzes the conversion of glucosylceramide to free glucose and ceramide.By similarity

    Catalytic activityi

    D-glucosyl-N-acylsphingosine + H2O = D-glucose + N-acylsphingosine.

    GO - Molecular functioni

    1. beta-glucosidase activity Source: UniProtKB
    2. glucosylceramidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. bile acid metabolic process Source: UniProtKB
    2. glucosylceramide catabolic process Source: InterPro
    3. glycoside catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Lipid metabolism, Sphingolipid metabolism

    Enzyme and pathway databases

    ReactomeiREACT_180747. Glycosphingolipid metabolism.

    Protein family/group databases

    CAZyiGH116. Glycoside Hydrolase Family 116.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Non-lysosomal glucosylceramidase (EC:3.2.1.45)
    Short name:
    NLGase
    Gene namesi
    ORF Names:CG33090
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 2L

    Organism-specific databases

    FlyBaseiFBgn0028916. CG33090.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 948948Non-lysosomal glucosylceramidasePRO_0000283761Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi200 – 2001N-linked (GlcNAc...)Sequence Analysis
    Modified residuei214 – 2141Phosphoserine1 Publication
    Glycosylationi288 – 2881N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi555 – 5551N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi629 – 6291N-linked (GlcNAc...)Sequence Analysis
    Modified residuei667 – 6671Phosphoserine1 Publication
    Modified residuei669 – 6691Phosphoserine1 Publication
    Glycosylationi673 – 6731N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiQ7KT91.
    PRIDEiQ7KT91.

    Expressioni

    Gene expression databases

    BgeeiQ7KT91.

    Interactioni

    Protein-protein interaction databases

    BioGridi60860. 1 interaction.
    STRINGi7227.FBpp0080197.

    Structurei

    3D structure databases

    ProteinModelPortaliQ7KT91.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 736736ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini754 – 948195CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei737 – 75317HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG4354.
    GeneTreeiENSGT00390000010998.
    InParanoidiQ95TX3.
    KOiK17108.
    OMAiEISITRC.
    OrthoDBiEOG7ZGX2B.
    PhylomeDBiQ7KT91.

    Family and domain databases

    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR014551. Beta_glucosidase_GBA2-type.
    IPR024462. GBA2_N.
    IPR006775. Glucosylceramidase.
    [Graphical view]
    PfamiPF04685. DUF608. 1 hit.
    PF12215. GBA2_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF028944. Beta_gluc_GBA2. 1 hit.
    SUPFAMiSSF48208. SSF48208. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Q7KT91-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAEPLAVETK PLSNGNANGN AVGIAESASA VFQEKLKLQQ QEESNEIAAV    50
    PKYGLKLKFD HVWPEKRIQN VRASIRQTLP MVPLVCRYAA YYWKVSREGR 100
    RVYMDYYYME NGKQIYGVPI GGIGGGTIGR GYAGEFCRFQ MRPGIYEYNV 150
    VLANQFIVTI KDPKGCTIFQ SLLSKCSTRD KTSDPDGDPD GERTKCQLPN 200
    CSSRAKQPLS AWHSNIEDTR CSYTGLYPRS WTEYDLSHYG VRLTCRQVSP 250
    VIPHEYRESS LPCAVFVWSV ENVCDQERKV SITFTFKNGT GNKKQDAEGG 300
    AESQLISEGN AKGVSIRQKI SEMPCSYNLA CRVLPEISIT RCPQFDPAGN 350
    GEQLWAQLKE HGQLSEHPTS EALKTKDIGV AVCGQVALKP MASHDLEFVL 400
    AWDMPKIQFP RKMQTHTRYY TKYFDDSGDS GPRICEYALR QYSTWERLID 450
    AWQRPILNDE TLPDWYKCAI FNQLYFISDG GTIWLKCDSS LGKELAYDDP 500
    RLAYGRFGYL EGHEYRMYNT YDVHFYASPA LAHLWPNLQV SLQYDFKDAI 550
    AAELNDTRKM LYDGKVMPRK VKNCVPHDLG DPDEEPFTLI NCYNIHDVND 600
    WKDLNTKFVL QVYRDYYVLN ELAQAQSDNA SKFSSIEFID KESLYELYSQ 650
    DNKRKNSADE KQQNRKSASM YINETNGKVY LMDAIGYLKA MYASCKAIME 700
    RTIEYDKDND GLIENTKMPD QTYDSWVMDG PSAYCSGLWL AALQAMSAMA 750
    TILDQPNDCL RYQDILEKGK RSLEEKLWNG SYYRFDLSHS HRDTIMADQL 800
    CGHWYLKSCG FDYEIYPKEN VRTALKRIYD NNVMGFHEGN IGAANGFIAN 850
    ASEPTKPGHV DNSNIQAEEV WPGVVYALAA TMIQEGMFEE AFQTAGGMYK 900
    TLSQRIGMNF ETPEALYGEK RYRSIGYMRP LSIWSMQVAL ERRRAQRD 948
    Length:948
    Mass (Da):108,291
    Last modified:July 5, 2004 - v1
    Checksum:i095184C4EC0F0DD7
    GO

    Sequence cautioni

    The sequence AAL13692.1 differs from that shown. Reason: Frameshift at position 887.
    The sequence ABC86280.1 differs from that shown. Reason: Frameshift at position 526.
    The sequence AAL13692.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014134 Genomic DNA. Translation: AAO41192.2.
    BT024218 mRNA. Translation: ABC86280.1. Frameshift.
    BT044085 mRNA. Translation: ACH92150.1.
    AY058463 mRNA. Translation: AAL13692.1. Sequence problems.
    RefSeqiNP_788055.2. NM_176041.1.
    UniGeneiDm.7918.

    Genome annotation databases

    EnsemblMetazoaiFBtr0080624; FBpp0080197; FBgn0028916.
    GeneIDi34835.
    KEGGidme:Dmel_CG33090.
    UCSCiCG33090-RB. d. melanogaster.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014134 Genomic DNA. Translation: AAO41192.2 .
    BT024218 mRNA. Translation: ABC86280.1 . Frameshift.
    BT044085 mRNA. Translation: ACH92150.1 .
    AY058463 mRNA. Translation: AAL13692.1 . Sequence problems.
    RefSeqi NP_788055.2. NM_176041.1.
    UniGenei Dm.7918.

    3D structure databases

    ProteinModelPortali Q7KT91.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 60860. 1 interaction.
    STRINGi 7227.FBpp0080197.

    Protein family/group databases

    CAZyi GH116. Glycoside Hydrolase Family 116.

    Proteomic databases

    PaxDbi Q7KT91.
    PRIDEi Q7KT91.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0080624 ; FBpp0080197 ; FBgn0028916 .
    GeneIDi 34835.
    KEGGi dme:Dmel_CG33090.
    UCSCi CG33090-RB. d. melanogaster.

    Organism-specific databases

    FlyBasei FBgn0028916. CG33090.

    Phylogenomic databases

    eggNOGi COG4354.
    GeneTreei ENSGT00390000010998.
    InParanoidi Q95TX3.
    KOi K17108.
    OMAi EISITRC.
    OrthoDBi EOG7ZGX2B.
    PhylomeDBi Q7KT91.

    Enzyme and pathway databases

    Reactomei REACT_180747. Glycosphingolipid metabolism.

    Miscellaneous databases

    GenomeRNAii 34835.
    NextBioi 790459.
    PROi Q7KT91.

    Gene expression databases

    Bgeei Q7KT91.

    Family and domain databases

    InterProi IPR008928. 6-hairpin_glycosidase-like.
    IPR014551. Beta_glucosidase_GBA2-type.
    IPR024462. GBA2_N.
    IPR006775. Glucosylceramidase.
    [Graphical view ]
    Pfami PF04685. DUF608. 1 hit.
    PF12215. GBA2_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF028944. Beta_gluc_GBA2. 1 hit.
    SUPFAMi SSF48208. SSF48208. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    2. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    3. Stapleton M., Carlson J.W., Booth B., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E.
      Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Berkeley.
      Tissue: Embryo.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 466-948.
      Strain: Berkeley.
      Tissue: Head.
    5. "Phosphoproteome analysis of Drosophila melanogaster embryos."
      Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
      J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-214; SER-667 AND SER-669, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Embryo.

    Entry informationi

    Entry nameiC3390_DROME
    AccessioniPrimary (citable) accession number: Q7KT91
    Secondary accession number(s): B5RIG1, Q29R22, Q95TX3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 3, 2007
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3