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Q7KT91 (C3390_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Non-lysosomal glucosylceramidase

Short name=NLGase
EC=3.2.1.45
Gene names
ORF Names:CG33090
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length948 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Non-lysosomal glucosylceramidase that catalyzes the conversion of glucosylceramide to free glucose and ceramide By similarity.

Catalytic activity

D-glucosyl-N-acylsphingosine + H2O = D-glucose + N-acylsphingosine.

Subcellular location

Cell membrane; Single-pass membrane protein Potential.

Sequence similarities

Belongs to the non-lysosomal glucosylceramidase family.

Sequence caution

The sequence AAL13692.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

The sequence AAL13692.1 differs from that shown. Reason: Frameshift at position 887.

The sequence ABC86280.1 differs from that shown. Reason: Frameshift at position 526.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 948948Non-lysosomal glucosylceramidase
PRO_0000283761

Regions

Topological domain1 – 736736Extracellular Potential
Transmembrane737 – 75317Helical; Potential
Topological domain754 – 948195Cytoplasmic Potential

Amino acid modifications

Modified residue2141Phosphoserine Ref.5
Modified residue6671Phosphoserine Ref.5
Modified residue6691Phosphoserine Ref.5
Glycosylation2001N-linked (GlcNAc...) Potential
Glycosylation2881N-linked (GlcNAc...) Potential
Glycosylation5551N-linked (GlcNAc...) Potential
Glycosylation6291N-linked (GlcNAc...) Potential
Glycosylation6731N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q7KT91 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 095184C4EC0F0DD7

FASTA948108,291
        10         20         30         40         50         60 
MAEPLAVETK PLSNGNANGN AVGIAESASA VFQEKLKLQQ QEESNEIAAV PKYGLKLKFD 

        70         80         90        100        110        120 
HVWPEKRIQN VRASIRQTLP MVPLVCRYAA YYWKVSREGR RVYMDYYYME NGKQIYGVPI 

       130        140        150        160        170        180 
GGIGGGTIGR GYAGEFCRFQ MRPGIYEYNV VLANQFIVTI KDPKGCTIFQ SLLSKCSTRD 

       190        200        210        220        230        240 
KTSDPDGDPD GERTKCQLPN CSSRAKQPLS AWHSNIEDTR CSYTGLYPRS WTEYDLSHYG 

       250        260        270        280        290        300 
VRLTCRQVSP VIPHEYRESS LPCAVFVWSV ENVCDQERKV SITFTFKNGT GNKKQDAEGG 

       310        320        330        340        350        360 
AESQLISEGN AKGVSIRQKI SEMPCSYNLA CRVLPEISIT RCPQFDPAGN GEQLWAQLKE 

       370        380        390        400        410        420 
HGQLSEHPTS EALKTKDIGV AVCGQVALKP MASHDLEFVL AWDMPKIQFP RKMQTHTRYY 

       430        440        450        460        470        480 
TKYFDDSGDS GPRICEYALR QYSTWERLID AWQRPILNDE TLPDWYKCAI FNQLYFISDG 

       490        500        510        520        530        540 
GTIWLKCDSS LGKELAYDDP RLAYGRFGYL EGHEYRMYNT YDVHFYASPA LAHLWPNLQV 

       550        560        570        580        590        600 
SLQYDFKDAI AAELNDTRKM LYDGKVMPRK VKNCVPHDLG DPDEEPFTLI NCYNIHDVND 

       610        620        630        640        650        660 
WKDLNTKFVL QVYRDYYVLN ELAQAQSDNA SKFSSIEFID KESLYELYSQ DNKRKNSADE 

       670        680        690        700        710        720 
KQQNRKSASM YINETNGKVY LMDAIGYLKA MYASCKAIME RTIEYDKDND GLIENTKMPD 

       730        740        750        760        770        780 
QTYDSWVMDG PSAYCSGLWL AALQAMSAMA TILDQPNDCL RYQDILEKGK RSLEEKLWNG 

       790        800        810        820        830        840 
SYYRFDLSHS HRDTIMADQL CGHWYLKSCG FDYEIYPKEN VRTALKRIYD NNVMGFHEGN 

       850        860        870        880        890        900 
IGAANGFIAN ASEPTKPGHV DNSNIQAEEV WPGVVYALAA TMIQEGMFEE AFQTAGGMYK 

       910        920        930        940 
TLSQRIGMNF ETPEALYGEK RYRSIGYMRP LSIWSMQVAL ERRRAQRD 

« Hide

References

« Hide 'large scale' references
[1]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[2]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: Berkeley.
[3]Stapleton M., Carlson J.W., Booth B., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E.
Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Berkeley.
Tissue: Embryo.
[4]"A Drosophila full-length cDNA resource."
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 466-948.
Strain: Berkeley.
Tissue: Head.
[5]"Phosphoproteome analysis of Drosophila melanogaster embryos."
Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-214; SER-667 AND SER-669, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Embryo.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014134 Genomic DNA. Translation: AAO41192.2.
BT024218 mRNA. Translation: ABC86280.1. Frameshift.
BT044085 mRNA. Translation: ACH92150.1.
AY058463 mRNA. Translation: AAL13692.1. Sequence problems.
RefSeqNP_788055.2. NM_176041.1.
UniGeneDm.7918.

3D structure databases

ProteinModelPortalQ7KT91.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid60860. 1 interaction.
STRING7227.FBpp0080197.

Protein family/group databases

CAZyGH116. Glycoside Hydrolase Family 116.

Proteomic databases

PaxDbQ7KT91.
PRIDEQ7KT91.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0080624; FBpp0080197; FBgn0028916.
GeneID34835.
KEGGdme:Dmel_CG33090.
UCSCCG33090-RB. d. melanogaster.

Organism-specific databases

FlyBaseFBgn0028916. CG33090.

Phylogenomic databases

eggNOGCOG4354.
GeneTreeENSGT00390000010998.
InParanoidQ95TX3.
KOK17108.
OMAEISITRC.
OrthoDBEOG7ZGX2B.
PhylomeDBQ7KT91.

Gene expression databases

BgeeQ7KT91.

Family and domain databases

InterProIPR008928. 6-hairpin_glycosidase-like.
IPR014551. Beta_glucosidase_GBA2-type.
IPR024462. GBA2_N.
IPR006775. Glucosylceramidase.
[Graphical view]
PfamPF04685. DUF608. 1 hit.
PF12215. GBA2_N. 1 hit.
[Graphical view]
PIRSFPIRSF028944. Beta_gluc_GBA2. 1 hit.
SUPFAMSSF48208. SSF48208. 2 hits.
ProtoNetSearch...

Other

GenomeRNAi34835.
NextBio790459.
PROQ7KT91.

Entry information

Entry nameC3390_DROME
AccessionPrimary (citable) accession number: Q7KT91
Secondary accession number(s): B5RIG1, Q29R22, Q95TX3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: July 5, 2004
Last modified: July 9, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase