Q7KT91 (C3390_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-lysosomal glucosylceramidase
Short name=NLGase
EC=3.2.1.45

Gene names

ORF Names:

CG33090

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	948 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Non-lysosomal glucosylceramidase that catalyzes the conversion of glucosylceramide to free glucose and ceramide By similarity.
Catalytic activity	D-glucosyl-N-acylsphingosine + H2O = D-glucose + N-acylsphingosine.
Subcellular location	Cell membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the non-lysosomal glucosylceramidase family.
Sequence caution	The sequence AAL13692.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence AAL13692.1 differs from that shown. Reason: Frameshift at position 887. The sequence ABC86280.1 differs from that shown. Reason: Frameshift at position 526.

Ontologies

Keywords
Biological process	Lipid metabolism Sphingolipid metabolism
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	bile acid metabolic process Inferred from sequence or structural similarity. Source: UniProtKB glucosylceramide catabolic process Inferred from electronic annotation. Source: InterPro glycoside catabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	integral to membrane Inferred from sequence or structural similarity. Source: UniProtKB plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	beta-glucosidase activity Inferred from sequence or structural similarity. Source: UniProtKB glucosylceramidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 948	948	Non-lysosomal glucosylceramidase		PRO_0000283761
Regions
	Topological domain	1 – 736	736	Extracellular Potential
	Transmembrane	737 – 753	17	Helical; Potential
	Topological domain	754 – 948	195	Cytoplasmic Potential
Amino acid modifications
	Modified residue	214	1	Phosphoserine Ref.5
	Modified residue	667	1	Phosphoserine Ref.5
	Modified residue	669	1	Phosphoserine Ref.5
	Glycosylation	200	1	N-linked (GlcNAc...) Potential
	Glycosylation	288	1	N-linked (GlcNAc...) Potential
	Glycosylation	555	1	N-linked (GlcNAc...) Potential
	Glycosylation	629	1	N-linked (GlcNAc...) Potential
	Glycosylation	673	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q7KT91 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 095184C4EC0F0DD7	FASTA	948	108,291
	10 20 30 40 50 60 MAEPLAVETK PLSNGNANGN AVGIAESASA VFQEKLKLQQ QEESNEIAAV PKYGLKLKFD 70 80 90 100 110 120 HVWPEKRIQN VRASIRQTLP MVPLVCRYAA YYWKVSREGR RVYMDYYYME NGKQIYGVPI 130 140 150 160 170 180 GGIGGGTIGR GYAGEFCRFQ MRPGIYEYNV VLANQFIVTI KDPKGCTIFQ SLLSKCSTRD 190 200 210 220 230 240 KTSDPDGDPD GERTKCQLPN CSSRAKQPLS AWHSNIEDTR CSYTGLYPRS WTEYDLSHYG 250 260 270 280 290 300 VRLTCRQVSP VIPHEYRESS LPCAVFVWSV ENVCDQERKV SITFTFKNGT GNKKQDAEGG 310 320 330 340 350 360 AESQLISEGN AKGVSIRQKI SEMPCSYNLA CRVLPEISIT RCPQFDPAGN GEQLWAQLKE 370 380 390 400 410 420 HGQLSEHPTS EALKTKDIGV AVCGQVALKP MASHDLEFVL AWDMPKIQFP RKMQTHTRYY 430 440 450 460 470 480 TKYFDDSGDS GPRICEYALR QYSTWERLID AWQRPILNDE TLPDWYKCAI FNQLYFISDG 490 500 510 520 530 540 GTIWLKCDSS LGKELAYDDP RLAYGRFGYL EGHEYRMYNT YDVHFYASPA LAHLWPNLQV 550 560 570 580 590 600 SLQYDFKDAI AAELNDTRKM LYDGKVMPRK VKNCVPHDLG DPDEEPFTLI NCYNIHDVND 610 620 630 640 650 660 WKDLNTKFVL QVYRDYYVLN ELAQAQSDNA SKFSSIEFID KESLYELYSQ DNKRKNSADE 670 680 690 700 710 720 KQQNRKSASM YINETNGKVY LMDAIGYLKA MYASCKAIME RTIEYDKDND GLIENTKMPD 730 740 750 760 770 780 QTYDSWVMDG PSAYCSGLWL AALQAMSAMA TILDQPNDCL RYQDILEKGK RSLEEKLWNG 790 800 810 820 830 840 SYYRFDLSHS HRDTIMADQL CGHWYLKSCG FDYEIYPKEN VRTALKRIYD NNVMGFHEGN 850 860 870 880 890 900 IGAANGFIAN ASEPTKPGHV DNSNIQAEEV WPGVVYALAA TMIQEGMFEE AFQTAGGMYK 910 920 930 940 TLSQRIGMNF ETPEALYGEK RYRSIGYMRP LSIWSMQVAL ERRRAQRD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[3]	Stapleton M., Carlson J.W., Booth B., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 466-948. Strain: Berkeley. Tissue: Head.
[5]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-214; SER-667 AND SER-669, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014134 Genomic DNA. Translation: AAO41192.2. BT024218 mRNA. Translation: ABC86280.1. Frameshift. BT044085 mRNA. Translation: ACH92150.1. AY058463 mRNA. Translation: AAL13692.1. Sequence problems.
RefSeq	NP_788055.2. NM_176041.1.
UniGene	Dm.7918.
3D structure databases
ProteinModelPortal	Q7KT91.
ModBase	Search...
Protein family/group databases
CAZy	GH116. Glycoside Hydrolase Family 116.
Proteomic databases
PaxDb	Q7KT91.
PRIDE	Q7KT91.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0080624; FBpp0080197; FBgn0028916.
GeneID	34835.
KEGG	dme:Dmel_CG33090.
UCSC	CG33090-RB. d. melanogaster.
Organism-specific databases
FlyBase	FBgn0028916. CG33090.
Phylogenomic databases
eggNOG	COG4354.
GeneTree	ENSGT00390000010998.
InParanoid	Q95TX3.
OMA	QRIGMNF.
OrthoDB	EOG4CFXQ4.
PhylomeDB	Q7KT91.
Gene expression databases
Bgee	Q7KT91.
Family and domain databases
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR014551. Beta_glucosidase_GBA2-type. IPR024462. GBA2_N. IPR006775. Glucosylceramidase. [Graphical view]
Pfam	PF04685. DUF608. 1 hit. PF12215. GBA2_N. 1 hit. [Graphical view]
PIRSF	PIRSF028944. Beta_gluc_GBA2. 1 hit.
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	34835.
NextBio	790459.

Entry information

Entry name

C3390_DROME

Accession

Primary (citable) accession number: Q7KT91
Secondary accession number(s): B5RIG1, Q29R22, Q95TX3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	July 5, 2004
Last modified:	April 3, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project