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Protein

Nucleosomal histone kinase 1

Gene

ball

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine kinase involved in somatic mitosis and female meiosis. Required for spindle organization in mitosis, and for the establishment or maintenance of meiosis-specific chromosomal configurations, including the prophase I karyosome and the metaphase I spindle. Specifically phosphorylates nucleosomal H2A on 'Thr-119'.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei77 – 771ATPPROSITE-ProRule annotation
Active sitei183 – 1831Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi53 – 619ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • histone threonine kinase activity Source: FlyBase
  • protein kinase activity Source: FlyBase

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • female germ-line stem cell population maintenance Source: FlyBase
  • female meiotic division Source: UniProtKB
  • histone phosphorylation Source: FlyBase
  • karyosome formation Source: FlyBase
  • male germ-line stem cell population maintenance Source: FlyBase
  • meiotic nuclear division Source: UniProtKB
  • mitotic sister chromatid segregation Source: UniProtKB
  • mitotic spindle organization Source: UniProtKB
  • neurogenesis Source: FlyBase
  • neuronal stem cell population maintenance Source: FlyBase
  • peptidyl-serine phosphorylation Source: GO_Central
  • peptidyl-threonine phosphorylation Source: FlyBase
  • protein phosphorylation Source: FlyBase
  • regulation of cell shape Source: GO_Central
  • regulation of response to DNA damage stimulus Source: FlyBase
  • synaptonemal complex assembly Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division, Meiosis, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-2995383. Initiation of Nuclear Envelope Reformation.
SignaLinkiQ7KRY6.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleosomal histone kinase 1 (EC:2.7.11.1)
Alternative name(s):
Protein baellchen
Gene namesi
Name:ball
Synonyms:ballchen, nhk-1
ORF Names:CG6386
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0027889. ball.

Subcellular locationi

GO - Cellular componenti

  • chromatin Source: UniProtKB
  • cytoplasm Source: GO_Central
  • nucleolus Source: FlyBase
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi117 – 1171P → L in Z3-0437; induces female and male sterility and abolishes T-119 phosphorylation of H2A in the oocyte. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 599599Nucleosomal histone kinase 1PRO_0000086441Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei376 – 3761Phosphoserine1 Publication
Modified residuei381 – 3811Phosphoserine1 Publication
Modified residuei382 – 3821Phosphoserine1 Publication
Modified residuei388 – 3881Phosphoserine1 Publication
Modified residuei390 – 3901Phosphoserine1 Publication
Modified residuei483 – 4831Phosphoserine1 Publication
Modified residuei564 – 5641Phosphoserine1 Publication
Modified residuei586 – 5861Phosphoserine1 Publication
Modified residuei589 – 5891Phosphothreonine1 Publication

Post-translational modificationi

Phosphorylated during mitosis and female meiosis.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ7KRY6.
PRIDEiQ7KRY6.

PTM databases

iPTMnetiQ7KRY6.

Expressioni

Tissue specificityi

Present in ovary (at protein level).2 Publications

Developmental stagei

Present throughout development, with highest level in early embryo (at protein level).1 Publication

Gene expression databases

BgeeiFBgn0027889.
ExpressionAtlasiQ7KRY6. differential.
GenevisibleiQ7KRY6. DM.

Interactioni

Protein-protein interaction databases

BioGridi68119. 7 interactions.
IntActiQ7KRY6. 3 interactions.
MINTiMINT-770900.
STRINGi7227.FBpp0084465.

Structurei

3D structure databases

ProteinModelPortaliQ7KRY6.
SMRiQ7KRY6. Positions 1-408.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini47 – 328282Protein kinasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi394 – 3985Poly-Glu

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1164. Eukaryota.
ENOG410XPGP. LUCA.
GeneTreeiENSGT00760000119040.
InParanoidiQ7KRY6.
KOiK08816.
OMAiAKINFSP.
OrthoDBiEOG091G05V5.
PhylomeDBiQ7KRY6.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7KRY6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRVAKPKAA APAKKVVSAK KAKSKLYKMP EKVKEGTVFT DLAKGQWRIG
60 70 80 90 100
PSIGVGGFGE IYAACKVGEK NYDAVVKCEP HGNGPLFVEM HFYLRNAKLE
110 120 130 140 150
DIKQFMQKHG LKSLGMPYIL ANGSVEVNGE KHRFIVMPRY GSDLTKFLEQ
160 170 180 190 200
NGKRLPEGTV YRLAIQMLDV YQYMHSNGYV HADLKAANIL LGLEKGGAAQ
210 220 230 240 250
AYLVDFGLAS HFVTGDFKPD PKKMHNGTIE YTSRDAHLGV PTRRADLEIL
260 270 280 290 300
GYNLIEWLGA ELPWVTQKLL AVPPKVQKAK EAFMDNIGES LKTLFPKGVP
310 320 330 340 350
PPIGDFMKYV SKLTHNQEPD YDKCRSWFSS ALKQLKIPNN GDLDFKMKPQ
360 370 380 390 400
TSSNNNLSPP GTSKAATARK AKKIDSPVLN SSLDEKISAS EDDEEEEEKS
410 420 430 440 450
HRKKTAKKVT PSARNAKVSP LKRVADSSPP SQKRVKTEPK STPRERATPK
460 470 480 490 500
ASPKPRSTPK ASPKPQTPTA ARLRTPNAKI NFSPSISLRG RPGGKTVIND
510 520 530 540 550
DLTPQPRSKK TYEFNFELDV SMDANVIVNV KRKKKADQDK ATAVDSRTPS
560 570 580 590
SRSALASSSK EEASPVTRVN LRKVNGHGDS STPGRSPRTP AVTVRKYQG
Length:599
Mass (Da):65,994
Last modified:July 5, 2004 - v1
Checksum:iB32EE3004477EBDB
GO

Sequence cautioni

The sequence AAD46857 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014297 Genomic DNA. Translation: AAF56630.2.
AF160917 mRNA. Translation: AAD46857.2. Different initiation.
RefSeqiNP_651508.1. NM_143251.1.
NP_733171.1. NM_170292.2.
UniGeneiDm.1346.

Genome annotation databases

EnsemblMetazoaiFBtr0085095; FBpp0084465; FBgn0027889.
FBtr0085096; FBpp0084466; FBgn0027889.
GeneIDi43228.
KEGGidme:Dmel_CG6386.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014297 Genomic DNA. Translation: AAF56630.2.
AF160917 mRNA. Translation: AAD46857.2. Different initiation.
RefSeqiNP_651508.1. NM_143251.1.
NP_733171.1. NM_170292.2.
UniGeneiDm.1346.

3D structure databases

ProteinModelPortaliQ7KRY6.
SMRiQ7KRY6. Positions 1-408.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi68119. 7 interactions.
IntActiQ7KRY6. 3 interactions.
MINTiMINT-770900.
STRINGi7227.FBpp0084465.

PTM databases

iPTMnetiQ7KRY6.

Proteomic databases

PaxDbiQ7KRY6.
PRIDEiQ7KRY6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0085095; FBpp0084465; FBgn0027889.
FBtr0085096; FBpp0084466; FBgn0027889.
GeneIDi43228.
KEGGidme:Dmel_CG6386.

Organism-specific databases

CTDi43228.
FlyBaseiFBgn0027889. ball.

Phylogenomic databases

eggNOGiKOG1164. Eukaryota.
ENOG410XPGP. LUCA.
GeneTreeiENSGT00760000119040.
InParanoidiQ7KRY6.
KOiK08816.
OMAiAKINFSP.
OrthoDBiEOG091G05V5.
PhylomeDBiQ7KRY6.

Enzyme and pathway databases

ReactomeiR-DME-2995383. Initiation of Nuclear Envelope Reformation.
SignaLinkiQ7KRY6.

Miscellaneous databases

GenomeRNAii43228.
PROiQ7KRY6.

Gene expression databases

BgeeiFBgn0027889.
ExpressionAtlasiQ7KRY6. differential.
GenevisibleiQ7KRY6. DM.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNHK1_DROME
AccessioniPrimary (citable) accession number: Q7KRY6
Secondary accession number(s): Q9U9Q0, Q9VBB2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: July 5, 2004
Last modified: September 7, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.