Reviewed,
UniProtKB/Swiss-Prot Q7KRY6 (NHK1_DROME)
Last modified
November 24, 2009.
Version 63.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Nucleosomal histone kinase 1 EC=2.7.11.1 Alternative name(s): Protein baellchen | ||||||
| Gene names |
| ||||||
| Organism | Drosophila melanogaster (Fruit fly) [Complete proteome] | ||||||
| Taxonomic identifier | 7227 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora |
Protein attributes
| Sequence length | 599 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Serine/threonine kinase involved in somatic mitosis and female meiosis. Required for spindle organization in mitosis, and for the establishment or maintenance of meiosis-specific chromosomal configurations, including the prophase I karyosome and the metaphase I spindle. Specifically phosphorylates nucleosomal H2A on 'Thr-119'. Ref.4 Ref.5 Ref.6 |
| Catalytic activity | ATP + a protein = ADP + a phosphoprotein. |
| Subcellular location | Cytoplasm. Nucleus. Note: Chromatin-associated during mitosis. Ref.4 |
| Tissue specificity | |
| Developmental stage | Present throughout development, with highest level in early embryo (at protein level). Ref.4 |
| Post-translational modification | Phosphorylated during mitosis and female meiosis. Ref.6 Ref.7 |
| Sequence similarities | Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. VRK subfamily. Contains 1 protein kinase domain. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| Q9W1K1 | 1 | EBI-170817,EBI-93866 | ||
| baf | Q9VLU0 | 1 | EBI-170817,EBI-105147 | |
| norpA | P13217 | 1 | EBI-170817,EBI-101510 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 599 | 599 | Nucleosomal histone kinase 1 | PRO_0000086441 | |||||
Regions | |||||||||
| Domain | 47 – 328 | 282 | Protein kinase | ||||||
| Nucleotide binding | 53 – 61 | 9 | ATP By similarity | ||||||
| Compositional bias | 394 – 398 | 5 | Poly-Glu | ||||||
Sites | |||||||||
| Active site | 183 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 77 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 376 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 381 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 382 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 388 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 390 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 483 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 564 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 586 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 589 | 1 | Phosphothreonine Ref.7 | ||||||
Experimental info | |||||||||
| Mutagenesis | 117 | 1 | P → L in Z3-0437; induces female and male sterility and abolishes T-119 phosphorylation of H2A in the oocyte. Ref.5 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. Venter J.C.Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley. |
| [2] | "Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. Lewis S.E.Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. |
| [3] | "A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo. |
| [4] | "Nucleosomal histone kinase-1 phosphorylates H2A Thr 119 during mitosis in the early Drosophila embryo." Aihara H., Nakagawa T., Yasui K., Ohta T., Hirose S., Dhomae N., Takio K., Kaneko M., Takeshima Y., Muramatsu M., Ito T. Genes Dev. 18:877-888(2004) [PubMed: 15078818] [Abstract] Cited for: PROTEIN SEQUENCE OF 35-44 AND 186-195, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, FUNCTION. Strain: Berkeley. Tissue: Embryo. |
| [5] | "A histone code in meiosis: the histone kinase, NHK-1, is required for proper chromosomal architecture in Drosophila oocytes." Ivanovska I., Khandan T., Ito T., Orr-Weaver T.L. Genes Dev. 19:2571-2582(2005) [PubMed: 16230526] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF PRO-117, TISSUE SPECIFICITY. |
| [6] | "The conserved kinase NHK-1 is essential for mitotic progression and unifying acentrosomal meiotic spindles in Drosophila melanogaster." Cullen C.F., Brittle A.L., Ito T., Ohkura H. J. Cell Biol. 171:593-602(2005) [PubMed: 16301329] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, PHOSPHORYLATION. |
| [7] | "Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-376; SER-381; SER-382; SER-388; SER-390; SER-483; SER-564; SER-586 AND THR-589, MASS SPECTROMETRY. Tissue: Embryo. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| AE014297 Genomic DNA. Translation: AAF56630.2. AF160917 mRNA. Translation: AAD46857.2. Different initiation. | |
| RefSeq | NP_651508.1. NP_733171.1. |
| UniGene | Dm.1346 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q7KRY6. 6 interactions. |
| STRING | Q7KRY6. |
Proteomic databases | |
| PRIDE | Q7KRY6. |
Genome annotation databases | |
| Ensembl | FBtr0085095; FBpp0084465; FBgn0027889; Drosophila melanogaster. [Genome view] FBtr0085096; FBpp0084466; FBgn0027889; Drosophila melanogaster. [Genome view] |
| GeneID | 43228. |
| KEGG | dme:Dmel_CG6386. |
Organism-specific databases | |
| CTD | 43228. |
| FlyBase | FBgn0027889. ball. |
Phylogenomic databases | |
| HOGENOM | Q7KRY6. |
| OMA | MIIKETS |
| OrthoDB | EOG94MXZK |
Enzyme and pathway databases | |
| BRENDA | 2.7.11.1. 48. |
Gene expression databases | |
| GermOnline | CG6386. Drosophila melanogaster. |
Family and domain databases | |
| InterPro | IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. [Graphical view] |
| Pfam | PF00069. Pkinase. 1 hit. [Graphical view] |
| PROSITE | PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 832818. |
Entry information
| Entry name | NHK1_DROME | ||||||||
| Accession | Primary (citable) accession number: Q7KRY6 Secondary accession number(s): Q9U9Q0, Q9VBB2 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | Drosophila annotation project | ||||||||
Relevant documents
| Drosophila Drosophila: entries, gene names and cross-references to FlyBase |
| SIMILARITY comments Index of protein domains and families |

Clusters with


