Q7KRY6 (NHK1_DROME) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 82.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Nucleosomal histone kinase 1 EC=2.7.11.1 Alternative name(s): Protein baellchen | ||||||
| Gene names |
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| Organism | Drosophila melanogaster (Fruit fly) | ||||||
| Taxonomic identifier | 7227 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora |
Protein attributes
| Sequence length | 599 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Serine/threonine kinase involved in somatic mitosis and female meiosis. Required for spindle organization in mitosis, and for the establishment or maintenance of meiosis-specific chromosomal configurations, including the prophase I karyosome and the metaphase I spindle. Specifically phosphorylates nucleosomal H2A on 'Thr-119'. Ref.4 Ref.5 Ref.6 |
| Catalytic activity | ATP + a protein = ADP + a phosphoprotein. |
| Subcellular location | Cytoplasm. Nucleus. Chromosome. Note: Chromatin-associated during mitosis. Ref.4 |
| Tissue specificity | |
| Developmental stage | Present throughout development, with highest level in early embryo (at protein level). Ref.4 |
| Post-translational modification | Phosphorylated during mitosis and female meiosis. Ref.6 Ref.7 |
| Sequence similarities | Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. VRK subfamily. Contains 1 protein kinase domain. |
| Sequence caution | The sequence AAD46857.2 differs from that shown. Reason: Erroneous initiation. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 599 | 599 | Nucleosomal histone kinase 1 | PRO_0000086441 | |||||
Regions | |||||||||
| Domain | 47 – 328 | 282 | Protein kinase | ||||||
| Nucleotide binding | 53 – 61 | 9 | ATP By similarity | ||||||
| Compositional bias | 394 – 398 | 5 | Poly-Glu | ||||||
Sites | |||||||||
| Active site | 183 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 77 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 376 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 381 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 382 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 388 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 390 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 483 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 564 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 586 | 1 | Phosphoserine Ref.7 | ||||||
| Modified residue | 589 | 1 | Phosphothreonine Ref.7 | ||||||
Experimental info | |||||||||
| Mutagenesis | 117 | 1 | P → L in Z3-0437; induces female and male sterility and abolishes T-119 phosphorylation of H2A in the oocyte. Ref.5 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. Venter J.C.Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley. |
| [2] | "Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. Lewis S.E.Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley. |
| [3] | "A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo. |
| [4] | "Nucleosomal histone kinase-1 phosphorylates H2A Thr 119 during mitosis in the early Drosophila embryo." Aihara H., Nakagawa T., Yasui K., Ohta T., Hirose S., Dhomae N., Takio K., Kaneko M., Takeshima Y., Muramatsu M., Ito T. Genes Dev. 18:877-888(2004) [PubMed: 15078818] [Abstract] Cited for: PROTEIN SEQUENCE OF 35-44 AND 186-195, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, FUNCTION. Strain: Berkeley. Tissue: Embryo. |
| [5] | "A histone code in meiosis: the histone kinase, NHK-1, is required for proper chromosomal architecture in Drosophila oocytes." Ivanovska I., Khandan T., Ito T., Orr-Weaver T.L. Genes Dev. 19:2571-2582(2005) [PubMed: 16230526] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF PRO-117, TISSUE SPECIFICITY. |
| [6] | "The conserved kinase NHK-1 is essential for mitotic progression and unifying acentrosomal meiotic spindles in Drosophila melanogaster." Cullen C.F., Brittle A.L., Ito T., Ohkura H. J. Cell Biol. 171:593-602(2005) [PubMed: 16301329] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, PHOSPHORYLATION. |
| [7] | "Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-376; SER-381; SER-382; SER-388; SER-390; SER-483; SER-564; SER-586 AND THR-589, MASS SPECTROMETRY. Tissue: Embryo. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AE014297 Genomic DNA. Translation: AAF56630.2. AF160917 mRNA. Translation: AAD46857.2. Different initiation. |
| RefSeq | NP_651508.1. NM_143251.1. NP_733171.1. NM_170292.1. |
| UniGene | Dm.1346. |
3D structure databases | |
| ProteinModelPortal | Q7KRY6. |
| SMR | Q7KRY6. Positions 31-344. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q7KRY6. 3 interactions. |
| MINT | MINT-770900. |
| STRING | Q7KRY6. |
Proteomic databases | |
| PRIDE | Q7KRY6. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblMetazoa | FBtr0085095; FBpp0084465; FBgn0027889. FBtr0085096; FBpp0084466; FBgn0027889. |
| GeneID | 43228. |
| KEGG | dme:Dmel_CG6386. |
Organism-specific databases | |
| CTD | 43228. |
| FlyBase | FBgn0027889. ball. |
Phylogenomic databases | |
| eggNOG | inNOG05461. |
| GeneTree | EMGT00070000025745. |
| InParanoid | Q7KRY6. |
| OMA | SMDANVI. |
| OrthoDB | EOG4JSXNJ. |
| PhylomeDB | Q7KRY6. |
Gene expression databases | |
| Bgee | Q7KRY6. |
| GermOnline | CG6386. Drosophila melanogaster. |
Family and domain databases | |
| InterPro | IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view] |
| KO | K08816. |
| Pfam | PF00069. Pkinase. 1 hit. [Graphical view] |
| SUPFAM | SSF56112. Kinase_like. 1 hit. |
| PROSITE | PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 832818. |
Entry information
| Entry name | NHK1_DROME | ||||||||
| Accession | Primary (citable) accession number: Q7KRY6 Secondary accession number(s): Q9U9Q0, Q9VBB2 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Drosophila annotation project | ||||||||
Relevant documents
| Drosophila Drosophila: entries, gene names and cross-references to FlyBase |
| SIMILARITY comments Index of protein domains and families |

Clusters with