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Protein

Nucleosomal histone kinase 1

Gene

ball

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase involved in somatic mitosis and female meiosis (PubMed:16230526). Required for spindle organization in mitosis, and for the establishment or maintenance of meiosis-specific chromosomal configurations, including the prophase I karyosome and the metaphase I spindle (PubMed:15078818, PubMed:16301329). Specifically phosphorylates nucleosomal H2A on 'Thr-119' (PubMed:15078818). Required for the development and organization of indirect flight muscle sarcomeres by regulating the formation of M line and H zone and the correct assembly of thick and thin filaments in the sarcomere (PubMed:26251439).4 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Cofactori

Mg2+1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei77ATPPROSITE-ProRule annotation1
Active sitei183Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi53 – 61ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • histone threonine kinase activity Source: FlyBase
  • metal ion binding Source: UniProtKB-KW
  • protein kinase activity Source: FlyBase
  • protein kinase binding Source: UniProtKB

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • chromosome condensation Source: FlyBase
  • female germ-line stem cell population maintenance Source: FlyBase
  • female meiotic nuclear division Source: UniProtKB
  • flight Source: UniProtKB
  • histone phosphorylation Source: FlyBase
  • karyosome formation Source: FlyBase
  • male germ-line stem cell population maintenance Source: FlyBase
  • meiotic cell cycle Source: UniProtKB
  • mitotic sister chromatid segregation Source: UniProtKB
  • mitotic spindle organization Source: UniProtKB
  • neuronal stem cell population maintenance Source: FlyBase
  • peptidyl-serine phosphorylation Source: GO_Central
  • peptidyl-threonine phosphorylation Source: FlyBase
  • protein phosphorylation Source: FlyBase
  • regulation of cell shape Source: GO_Central
  • regulation of response to DNA damage stimulus Source: FlyBase
  • sarcomere organization Source: FlyBase
  • synaptonemal complex assembly Source: UniProtKB

Keywordsi

Molecular functionChromatin regulator, Kinase, Serine/threonine-protein kinase, Transferase
Biological processCell cycle, Cell division, Meiosis, Mitosis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-202670. ERKs are inactivated.
R-DME-2995383. Initiation of Nuclear Envelope Reformation.
SignaLinkiQ7KRY6.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleosomal histone kinase 1 (EC:2.7.11.11 Publication)
Alternative name(s):
Protein baellchen
Gene namesi
Name:ball
Synonyms:ballchen, nhk-1
ORF Names:CG6386
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0027889. ball.

Subcellular locationi

GO - Cellular componenti

  • chromatin Source: UniProtKB
  • M band Source: UniProtKB
  • nucleolus Source: FlyBase
  • nucleus Source: UniProtKB
  • sarcomere Source: FlyBase
  • Z disc Source: UniProtKB

Keywords - Cellular componenti

Chromosome, Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown in muscles causes 91 percent lethality at the early pupal stage and the few surviving animals cannot fly. Severe defects in the indirect flight muscle structure characterized by narrower and wavy myofibrils and abnormal positioning of sarcomere Z line, M line and H line. Although the spacing between Z line and M lines stays regular, in less affected myobrils Z lines and M lines appear fragmented, Z lines are diffused at the edges and sarcomere length is shorter. In the more affected myofibrils, rudimentary Z lines are shifted into the region of thick and thin filament overlap and are by-passed by abnormally long filaments and H zones fail to localize to the middle of the sarcomere. Localization of unc-89/obscurin to M lines and localization of kettin (sls isoform A) to Z line is not affected.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi117P → L in Z3-0437; induces female and male sterility and abolishes T-119 phosphorylation of H2A in the oocyte. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000864411 – 599Nucleosomal histone kinase 1Add BLAST599

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei376Phosphoserine1 Publication1
Modified residuei381Phosphoserine1 Publication1
Modified residuei382Phosphoserine1 Publication1
Modified residuei388Phosphoserine1 Publication1
Modified residuei390Phosphoserine1 Publication1
Modified residuei483Phosphoserine1 Publication1
Modified residuei564Phosphoserine1 Publication1
Modified residuei586Phosphoserine1 Publication1
Modified residuei589Phosphothreonine1 Publication1

Post-translational modificationi

Phosphorylated during mitosis and female meiosis.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ7KRY6.
PRIDEiQ7KRY6.

PTM databases

iPTMnetiQ7KRY6.

Expressioni

Tissue specificityi

Expressed in ovaries (at protein level) (PubMed:16230526, PubMed:16301329). Expressed in indirect flight muscle (IFM) (at protein level) (PubMed:26251439).3 Publications

Developmental stagei

Present throughout development, with highest level in early embryo (at protein level).1 Publication

Gene expression databases

BgeeiFBgn0027889.
ExpressionAtlasiQ7KRY6. differential.
GenevisibleiQ7KRY6. DM.

Interactioni

Subunit structurei

May interact with Unc-89 (via protein kinase domain 1).1 Publication

GO - Molecular functioni

  • protein kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi68119. 7 interactors.
IntActiQ7KRY6. 3 interactors.
MINTiMINT-770900.
STRINGi7227.FBpp0084465.

Structurei

3D structure databases

ProteinModelPortaliQ7KRY6.
SMRiQ7KRY6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini47 – 328Protein kinasePROSITE-ProRule annotationAdd BLAST282

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi394 – 398Poly-Glu5

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1164. Eukaryota.
ENOG410XPGP. LUCA.
InParanoidiQ7KRY6.
KOiK08816.
OMAiAKQDDIK.
OrthoDBiEOG091G05V5.
PhylomeDBiQ7KRY6.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7KRY6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRVAKPKAA APAKKVVSAK KAKSKLYKMP EKVKEGTVFT DLAKGQWRIG
60 70 80 90 100
PSIGVGGFGE IYAACKVGEK NYDAVVKCEP HGNGPLFVEM HFYLRNAKLE
110 120 130 140 150
DIKQFMQKHG LKSLGMPYIL ANGSVEVNGE KHRFIVMPRY GSDLTKFLEQ
160 170 180 190 200
NGKRLPEGTV YRLAIQMLDV YQYMHSNGYV HADLKAANIL LGLEKGGAAQ
210 220 230 240 250
AYLVDFGLAS HFVTGDFKPD PKKMHNGTIE YTSRDAHLGV PTRRADLEIL
260 270 280 290 300
GYNLIEWLGA ELPWVTQKLL AVPPKVQKAK EAFMDNIGES LKTLFPKGVP
310 320 330 340 350
PPIGDFMKYV SKLTHNQEPD YDKCRSWFSS ALKQLKIPNN GDLDFKMKPQ
360 370 380 390 400
TSSNNNLSPP GTSKAATARK AKKIDSPVLN SSLDEKISAS EDDEEEEEKS
410 420 430 440 450
HRKKTAKKVT PSARNAKVSP LKRVADSSPP SQKRVKTEPK STPRERATPK
460 470 480 490 500
ASPKPRSTPK ASPKPQTPTA ARLRTPNAKI NFSPSISLRG RPGGKTVIND
510 520 530 540 550
DLTPQPRSKK TYEFNFELDV SMDANVIVNV KRKKKADQDK ATAVDSRTPS
560 570 580 590
SRSALASSSK EEASPVTRVN LRKVNGHGDS STPGRSPRTP AVTVRKYQG
Length:599
Mass (Da):65,994
Last modified:July 5, 2004 - v1
Checksum:iB32EE3004477EBDB
GO

Sequence cautioni

The sequence AAD46857 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014297 Genomic DNA. Translation: AAF56630.2.
AF160917 mRNA. Translation: AAD46857.2. Different initiation.
RefSeqiNP_651508.1. NM_143251.1.
NP_733171.1. NM_170292.2.
UniGeneiDm.1346.

Genome annotation databases

EnsemblMetazoaiFBtr0085095; FBpp0084465; FBgn0027889.
FBtr0085096; FBpp0084466; FBgn0027889.
GeneIDi43228.
KEGGidme:Dmel_CG6386.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiNHK1_DROME
AccessioniPrimary (citable) accession number: Q7KRY6
Secondary accession number(s): Q9U9Q0, Q9VBB2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: July 5, 2004
Last modified: July 5, 2017
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families