Reviewed, UniProtKB/Swiss-Prot Q7KQM4 (PLM1_PLAF7)

Names and origin

Protein names

Recommended name:
    Plasmepsin-1
    EC=3.4.23.38
Alternative name(s):
    Aspartic hemoglobinase I
    PfAPG

Gene names

ORF Names:

PF14_0076

Organism

Plasmodium falciparum (isolate 3D7)

Taxonomic identifier

36329 [NCBI]

Taxonomic lineage

Eukaryota › Alveolata › Apicomplexa › Aconoidasida › Haemosporida › Plasmodium › Plasmodium (Laverania)

Protein attributes

Sequence length	452 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Participates in the digestion of the host hemoglobin. Initial cleavage at the hinge region of hemoglobin, than cleaves at other sites, leading to denaturation of the molecule and to further degradation By similarity.
Catalytic activity	Hydrolysis of the 33-Phe-|-Leu-34 bond in the alpha-chain of hemoglobin, leading to denaturation of molecule.
Subcellular location	Vacuole By similarity. Note: Could be first anchored to the membrane through its propeptide before being released By similarity.
Developmental stage	Erythrocytic stages.
Sequence similarities	Belongs to the peptidase A1 family.

Ontologies

Keywords
Cellular component	Vacuole
Domain	Signal
Molecular function	Aspartyl protease Hydrolase Protease
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	vacuole Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – ?		Potential
	Propeptide	? – 124		Activation peptide By similarity		PRO_0000233400
	Chain	125 – 452	328	Plasmepsin-1		PRO_0000233401
Sites
	Active site	157	1	By similarity
	Active site	337	1	By similarity
Amino acid modifications
	Glycosylation	184	1	N-linked (GlcNAc...) Potential
	Glycosylation	218	1	N-linked (GlcNAc...) Potential
	Glycosylation	326	1	N-linked (GlcNAc...) Potential
	Glycosylation	440	1	N-linked (GlcNAc...) Potential
	Disulfide bond	170 ↔ 175		By similarity
	Disulfide bond	372 ↔ 408		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7KQM4-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 8F8F8478F2F7D931	FASTA	452	51,461
	10 20 30 40 50 60 MALSIKEDFS SAFAKNESAV NSSTFNNNMK TWKIQKRFQI LYVFFFLLIT GALFYYLIDN 70 80 90 100 110 120 VLFPKNKKIN EIMNTSKHVI IGFSIENSHD RIMKTVKQHR LKNYIKESLK FFKTGLTQKP 130 140 150 160 170 180 HLGNAGDSVT LNDVANVMYY GEAQIGDNKQ KFAFIFDTGS ANLWVPSAQC NTIGCKTKNL 190 200 210 220 230 240 YDSNKSKTYE KDGTKVEMNY VSGTVSGFFS KDIVTIANLS FPYKFIEVTD TNGFEPAYTL 250 260 270 280 290 300 GQFDGIVGLG WKDLSIGSVD PVVVELKNQN KIEQAVFTFY LPFDDKHKGY LTIGGIEDRF 310 320 330 340 350 360 YEGQLTYEKL NHDLYWQVDL DLHFGNLTVE KATAIVDSGT SSITAPTEFL NKFFEGLDVV 370 380 390 400 410 420 KIPFLPLYIT TCNNPKLPTL EFRSATNVYT LEPEYYLQQI FDFGISLCMV SIIPVDLNKN 430 440 450 TFILGDPFMR KYFTVFDYDN HTVGFALAKK KL

References

[1]

"Genome sequence of the human malaria parasite Plasmodium falciparum." Gardner M.J., Hall N., Fung E., White O., Berriman M., Hyman R.W., Carlton J.M., Pain A., Nelson K.E., Bowman S., Paulsen I.T., James K.D., Eisen J.A., Rutherford K.M., Salzberg S.L., Craig A., Kyes S., Chan M.-S. , Nene V., Shallom S.J., Suh B., Peterson J., Angiuoli S., Pertea M., Allen J., Selengut J., Haft D., Mather M.W., Vaidya A.B., Martin D.M.A., Fairlamb A.H., Fraunholz M.J., Roos D.S., Ralph S.A., McFadden G.I., Cummings L.M., Subramanian G.M., Mungall C., Venter J.C., Carucci D.J., Hoffman S.L., Newbold C., Davis R.W., Fraser C.M., Barrell B.G. Nature 419:498-511(2002) [PubMed: 12368864] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014817 Genomic DNA. Translation: AAN36688.1.
RefSeq	XP_001348249.1.
3D structure databases
HSSP	HSSP built from PDB template 1BBS based on UniProtKB P00797.
ModBase	Search...
Genome annotation databases
GeneID	811658.
KEGG	pfa:PF14_0076.
NMPDR	fig|36329.1.peg.1951.
Phylogenomic databases
HOGENOM	Q7KQM4.
OMA	Q7KQM4. RTISITY.
Family and domain databases
InterPro	IPR001461. Peptidase_A1. IPR001969. Peptidase_aspartic_AS. IPR009007. Peptidase_aspartic_catalytic. [Graphical view]
Gene3D	G3DSA:2.40.70.10. Pept_Aspartc_cat. 1 hit.
PANTHER	PTHR13683. Peptidase_A1. 1 hit.
Pfam	PF00026. Asp. 1 hit. [Graphical view]
PRINTS	PR00792. PEPSIN.
PROSITE	PS00141. ASP_PROTEASE. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
BindingDB	Q7KQM4.

Entry information

Entry name

PLM1_PLAF7

Accession

Primary (citable) accession number: Q7KQM4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	July 5, 2004
Last modified:	June 16, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)