Q7KQL9 (ALF_PLAF7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fructose-bisphosphate aldolase
EC=4.1.2.13

Gene names

ORF Names:

PF14_0425

Organism

Plasmodium falciparum (isolate 3D7) [Reference proteome]

Taxonomic identifier

36329 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Alveolata › Apicomplexa › Aconoidasida › Haemosporida › Plasmodium › Plasmodium (Laverania) › Plasmodium falciparum

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the class I fructose-bisphosphate aldolase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	gluconeogenesis Traceable author statement PubMed 2190085. Source: GeneDB_Pfalciparum glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	fructose-bisphosphate aldolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 369	369	Fructose-bisphosphate aldolase		PRO_0000233386
Sites
	Active site	195	1	Proton acceptor By similarity
	Active site	237	1	Schiff-base intermediate with dihydroxyacetone-P By similarity
	Binding site	62	1	Substrate By similarity
	Binding site	152	1	Substrate By similarity
	Site	369	1	Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7KQL9 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 2AE9CDED4F5C96A4	FASTA	369	40,105
	10 20 30 40 50 60 MAHCTEYMNA PKKLPADVAE ELATTAQKLV QAGKGILAAD ESTQTIKKRF DNIKLENTIE 70 80 90 100 110 120 NRASYRDLLF GTKGLGKFIS GAILFEETLF QKNEAGVPMV NLLHNENIIP GIKVDKGLVN 130 140 150 160 170 180 IPCTDEEKST QGLDGLAERC KEYYKAGARF AKWRTVLVID TAKGKPTDLS IHETAWGLAR 190 200 210 220 230 240 YASICQQNRL VPIVEPEILA DGPHSIEVCA VVTQKVLSCV FKALQENGVL LEGALLKPNM 250 260 270 280 290 300 VTAGYECTAK TTTQDVGFLT VRTLRRTVPP ALPGVVFLSG GQSEEEASVN LNSINALGPH 310 320 330 340 350 360 PWALTFSYGR ALQASVLNTW QGKKENVAKA REVLLQRAEA NSLATYGKYK GGAGGENAGA SLYEKKYVY

References

[1]

"Genome sequence of the human malaria parasite Plasmodium falciparum." Gardner M.J., Hall N., Fung E., White O., Berriman M., Hyman R.W., Carlton J.M., Pain A., Nelson K.E., Bowman S., Paulsen I.T., James K.D., Eisen J.A., Rutherford K.M., Salzberg S.L., Craig A., Kyes S., Chan M.-S. , Nene V., Shallom S.J., Suh B., Peterson J., Angiuoli S., Pertea M., Allen J., Selengut J., Haft D., Mather M.W., Vaidya A.B., Martin D.M.A., Fairlamb A.H., Fraunholz M.J., Roos D.S., Ralph S.A., McFadden G.I., Cummings L.M., Subramanian G.M., Mungall C., Venter J.C., Carucci D.J., Hoffman S.L., Newbold C., Davis R.W., Fraser C.M., Barrell B.G. Nature 419:498-511(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Isolate 3D7.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014187 Genomic DNA. Translation: AAN37038.1.
RefSeq	XP_001348599.1. XM_001348563.2.
3D structure databases
ProteinModelPortal	Q7KQL9.
SMR	Q7KQL9. Positions 3-367.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q7KQL9. 4 interactions.
STRING	5833.PF14_0425-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	PF14_0425:mRNA; PF14_0425:pep; PF14_0425.
GeneID	812007.
KEGG	pfa:PF14_0425.
Organism-specific databases
EuPathDB	PlasmoDB:PF3D7_1444800.
Phylogenomic databases
HOGENOM	HOG000220876.
KO	K01623.
OMA	AERCKEY.
ProtClustDB	PTZ00019.
Enzyme and pathway databases
UniPathway	UPA00109; UER00183.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR000741. Aldolase_I. IPR013785. Aldolase_TIM. [Graphical view]
PANTHER	PTHR11627. PTHR11627. 1 hit.
Pfam	PF00274. Glycolytic. 1 hit. [Graphical view]
PROSITE	PS00158. ALDOLASE_CLASS_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALF_PLAF7

Accession

Primary (citable) accession number: Q7KQL9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2006
Last sequence update:	July 5, 2004
Last modified:	April 3, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)