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Protein

SUMO-conjugating enzyme

Gene

lwr

Organism
Drosophila melanogaster (Fruit fly)
Status
Unreviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + SUMO + protein lysine = AMP + diphosphate + protein N-SUMOyllysine.UniRule annotation

Pathwayi: protein sumoylation

This protein is involved in the pathway protein sumoylation, which is part of Protein modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway protein sumoylation and in Protein modification.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • heat shock protein binding Source: FlyBase
  • SUMO ligase activity Source: FlyBase
  • SUMO transferase activity Source: FlyBase
  • transcription factor binding Source: FlyBase
  • ubiquitin activating enzyme binding Source: FlyBase

GO - Biological processi

  • cellular protein modification process Source: FlyBase
  • cellular response to transforming growth factor beta stimulus Source: FlyBase
  • dorsal closure Source: FlyBase
  • female meiosis chromosome segregation Source: FlyBase
  • female meiotic division Source: FlyBase
  • germ cell migration Source: FlyBase
  • hemocyte proliferation Source: FlyBase
  • humoral immune response Source: FlyBase
  • mitotic G2 DNA damage checkpoint Source: FlyBase
  • negative regulation of hemocyte proliferation Source: FlyBase
  • negative regulation of lamellocyte differentiation Source: FlyBase
  • negative regulation of Toll signaling pathway Source: FlyBase
  • protein import into nucleus Source: FlyBase
  • protein sumoylation Source: FlyBase
  • spectrosome organization Source: FlyBase
  • zygotic specification of dorsal/ventral axis Source: FlyBase
Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathwayUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-3108214. SUMOylation of DNA damage response and repair proteins.
R-DME-3232118. SUMOylation of transcription factors.
R-DME-4570464. SUMOylation of RNA binding proteins.
R-DME-4615885. SUMOylation of DNA replication proteins.
UniPathwayiUPA00886.

Names & Taxonomyi

Protein namesi
Recommended name:
SUMO-conjugating enzymeUniRule annotation (EC:6.3.2.-UniRule annotation)
Gene namesi
Name:lwrImported
Synonyms:semiImported, UBC9Imported, Ubc9Imported
ORF Names:CG3018Imported, Dmel_CG3018Imported
OrganismiDrosophila melanogaster (Fruit fly)Imported
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0010602. lwr.

Subcellular locationi

GO - Cellular componenti

  • condensed chromosome outer kinetochore Source: FlyBase
  • condensed nuclear chromosome, centromeric region Source: FlyBase
  • cytoplasm Source: GO_Central
  • nucleus Source: FlyBase
Complete GO annotation...

Interactioni

GO - Molecular functioni

  • heat shock protein binding Source: FlyBase
  • transcription factor binding Source: FlyBase
  • ubiquitin activating enzyme binding Source: FlyBase

Protein-protein interaction databases

STRINGi7227.FBpp0301731.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 146140UBIQUITIN_CONJUGAT_2InterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiKOG0424. Eukaryota.
COG5078. LUCA.
GeneTreeiENSGT00550000075088.
KOiK10577.
OMAiDLKRWEC.
OrthoDBiEOG7B8S5F.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR027230. Ubc9.
IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PANTHERiPTHR24067:SF51. PTHR24067:SF51. 1 hit.
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7KNM2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGIAITRLG EERKAWRKDH PFGFVARPAK NPDGTLNLMI WECAIPGKKS
60 70 80 90 100
TPWEGGLYKL RMIFKDDYPT SPPKCKFEPP LFHPNVYPSG TVCLSLLDEE
110 120 130 140 150
KDWRPAITIK QILLGIQDLL NEPNIKDPAQ AEAYTIYCQN RLEYEKRVRA

QARAMAATE
Length:159
Mass (Da):18,148
Last modified:October 17, 2006 - v1
Checksum:i86FE1870845CFEE3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF030443 Genomic DNA. Translation: AAC38964.1.
AF030444 mRNA. Translation: AAC38965.1.
AF106664 Genomic DNA. Translation: AAD21970.1.
AF218861 mRNA. Translation: AAF31701.1.
AE014134 Genomic DNA. Translation: AAF51487.1.
AY060944 mRNA. Translation: AAL28492.1.
AY113433 mRNA. Translation: AAM29438.1.
AE014134 Genomic DNA. Translation: AAN10499.1.
AE014134 Genomic DNA. Translation: AGB92370.1.
AE014134 Genomic DNA. Translation: AGB92371.1.
AB017606 mRNA. Translation: BAA34574.1.
AB017607 Genomic DNA. Translation: BAA34575.1.
PIRiJC5970.
RefSeqiNP_001259833.1. NM_001272904.1.
NP_001259834.1. NM_001272905.1.
NP_476978.1. NM_057630.4.
NP_722637.1. NM_164385.2.
UniGeneiDm.3336.

Genome annotation databases

EnsemblMetazoaiFBtr0078081; FBpp0077741; FBgn0010602.
FBtr0078082; FBpp0077742; FBgn0010602.
FBtr0310028; FBpp0301731; FBgn0010602.
FBtr0310029; FBpp0301732; FBgn0010602.
GeneIDi33226.
KEGGidme:Dmel_CG3018.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF030443 Genomic DNA. Translation: AAC38964.1.
AF030444 mRNA. Translation: AAC38965.1.
AF106664 Genomic DNA. Translation: AAD21970.1.
AF218861 mRNA. Translation: AAF31701.1.
AE014134 Genomic DNA. Translation: AAF51487.1.
AY060944 mRNA. Translation: AAL28492.1.
AY113433 mRNA. Translation: AAM29438.1.
AE014134 Genomic DNA. Translation: AAN10499.1.
AE014134 Genomic DNA. Translation: AGB92370.1.
AE014134 Genomic DNA. Translation: AGB92371.1.
AB017606 mRNA. Translation: BAA34574.1.
AB017607 Genomic DNA. Translation: BAA34575.1.
PIRiJC5970.
RefSeqiNP_001259833.1. NM_001272904.1.
NP_001259834.1. NM_001272905.1.
NP_476978.1. NM_057630.4.
NP_722637.1. NM_164385.2.
UniGeneiDm.3336.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7227.FBpp0301731.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0078081; FBpp0077741; FBgn0010602.
FBtr0078082; FBpp0077742; FBgn0010602.
FBtr0310028; FBpp0301731; FBgn0010602.
FBtr0310029; FBpp0301732; FBgn0010602.
GeneIDi33226.
KEGGidme:Dmel_CG3018.

Organism-specific databases

CTDi33226.
FlyBaseiFBgn0010602. lwr.

Phylogenomic databases

eggNOGiKOG0424. Eukaryota.
COG5078. LUCA.
GeneTreeiENSGT00550000075088.
KOiK10577.
OMAiDLKRWEC.
OrthoDBiEOG7B8S5F.

Enzyme and pathway databases

UniPathwayiUPA00886.
ReactomeiR-DME-3108214. SUMOylation of DNA damage response and repair proteins.
R-DME-3232118. SUMOylation of transcription factors.
R-DME-4570464. SUMOylation of RNA binding proteins.
R-DME-4615885. SUMOylation of DNA replication proteins.

Miscellaneous databases

ChiTaRSilwr. fly.
GenomeRNAii33226.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR027230. Ubc9.
IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PANTHERiPTHR24067:SF51. PTHR24067:SF51. 1 hit.
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and developmental expression of a nuclear ubiquitin-conjugating enzyme (DmUbc9) that interacts with small heat shock proteins in Drosophila melanogaster."
    Joanisse D.R., Inaguma Y., Tanguay R.M.
    Biochem. Biophys. Res. Commun. 244:102-109(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  2. "The Drosophila semushi mutation blocks nuclear import of bicoid during embryogenesis."
    Epps J.L., Tanda S.
    Curr. Biol. 8:1277-1280(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  3. "Identification and characterization of a Drosophila homologue of the yeast UBC9 and hus genes."
    Ohsako S., Takamatsu Y.
    Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: Canton SImported.
  4. "Identification and characterization of a Drosophila homologue of the yeast UBC9 and hus5 genes."
    Ohsako S., Takamatsu Y.
    J. Biochem. 125:230-235(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: oregon RImported.
  5. "A functional interaction between dorsal and components of the Smt3 conjugation machinery."
    Bhaskar V., Valentine S.A., Courey A.J.
    J. Biol. Chem. 275:4033-4040(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  6. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.H., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Gabor G.L., Abril J.F., Agbayani A., An H.J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., WoodageT, Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BerkeleyImported.
  7. Cited for: NUCLEOTIDE SEQUENCE.
  8. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BerkeleyImported.
  9. Cited for: GENOME REANNOTATION.
    Strain: BerkeleyImported.
  10. "The transposable elements of the Drosophila melanogaster euchromatin: a genomics perspective."
    Kaminker J.S., Bergman C.M., Kronmiller B., Carlson J., Svirskas R., Patel S., Frise E., Wheeler D.A., Lewis S.E., Rubin G.M., Ashburner M., Celniker S.E.
    Genome Biol. 3:RESEARCH0084.1-RESEARCH0084.20(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BerkeleyImported.
  11. "Heterochromatic sequences in a Drosophila whole-genome shotgun assembly."
    Hoskins R.A., Smith C.D., Carlson J.W., Carvalho A.B., Halpern A., Kaminker J.S., Kennedy C., Mungall C.J., Sullivan B.A., Sutton G.G., Yasuhara J.C., Wakimoto B.T., Myers E.W., Celniker S.E., Rubin G.M., Karpen G.H.
    Genome Biol. 3:RESEARCH0085-RESEARCH0085(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BerkeleyImported.
  12. Cited for: NUCLEOTIDE SEQUENCE.
    Strain: BerkeleyImported.
  13. "Combined evidence annotation of transposable elements in genome sequences."
    Quesneville H., Bergman C.M., Andrieu O., Autard D., Nouaud D., Ashburner M., Anxolabehere D.
    PLoS Comput. Biol. 1:166-175(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BerkeleyImported.
  14. O'Boyle S.T.
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
  15. Celniker S., Carlson J., Wan K., Frise E., Hoskins R., Park S., Svirskas R., Rubin G.
    Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
  16. "Drosophila melanogaster release 4 sequence."
    Berkeley Drosophila Genome Project
    Celniker S., Carlson J., Wan K., Pfeiffer B., Frise E., George R., Hoskins R., Stapleton M., Pacleb J., Park S., Svirskas R., Smith E., Yu C., Rubin G.
    Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
  17. "The Release 5.1 annotation of Drosophila melanogaster heterochromatin."
    Smith C.D., Shu S., Mungall C.J., Karpen G.H.
    Science 316:1586-1591(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BerkeleyImported.
  18. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BerkeleyImported.
  19. Millard Andrew
    Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.

Entry informationi

Entry nameiQ7KNM2_DROME
AccessioniPrimary (citable) accession number: Q7KNM2
Entry historyi
Integrated into UniProtKB/TrEMBL: October 17, 2006
Last sequence update: October 17, 2006
Last modified: July 6, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Proteomics identificationCombined sources, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.