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Protein

26S proteasome non-ATPase regulatory subunit 11

Gene

Rpn6

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the lid subcomplex of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. In the complex, RPN6 is required for proteasome assembly (By similarity). May act as linker between 19S regulatory subunit and the 20S proteasome core.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-DME-1169091. Activation of NF-kappaB in B cells.
R-DME-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-DME-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-DME-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-DME-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-DME-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-DME-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-DME-195253. Degradation of beta-catenin by the destruction complex.
R-DME-202424. Downstream TCR signaling.
R-DME-2871837. FCERI mediated NF-kB activation.
R-DME-350562. Regulation of ornithine decarboxylase (ODC).
R-DME-446652. Interleukin-1 family signaling.
R-DME-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-DME-4608870. Asymmetric localization of PCP proteins.
R-DME-4641257. Degradation of AXIN.
R-DME-4641258. Degradation of DVL.
R-DME-5358346. Hedgehog ligand biogenesis.
R-DME-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-DME-5607764. CLEC7A (Dectin-1) signaling.
R-DME-5610780. Degradation of GLI1 by the proteasome.
R-DME-5610785. GLI3 is processed to GLI3R by the proteasome.
R-DME-5632684. Hedgehog 'on' state.
R-DME-5658442. Regulation of RAS by GAPs.
R-DME-5676590. NIK-->noncanonical NF-kB signaling.
R-DME-5689603. UCH proteinases.
R-DME-5689880. Ub-specific processing proteases.
R-DME-6798695. Neutrophil degranulation.
R-DME-68949. Orc1 removal from chromatin.
R-DME-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-DME-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-DME-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-DME-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-DME-8941858. Regulation of RUNX3 expression and activity.
R-DME-8948751. Regulation of PTEN stability and activity.
R-DME-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome non-ATPase regulatory subunit 11
Alternative name(s):
26S proteasome regulatory complex subunit p42B
26S proteasome regulatory subunit Rpn6
Gene namesi
Name:Rpn6
ORF Names:CG10149
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0028689. Rpn6.

Subcellular locationi

Keywords - Cellular componenti

Proteasome

Pathology & Biotechi

Disruption phenotypei

Embryos develop and hatch normally but fail to outlive early larval stages. During the first and second instar larval stages larvae become immobile and sluggish and die without any observable defects. Lethality is probably the result of additive defects in overall cell proliferation rather than of distinct developmental disorders.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004199821 – 42226S proteasome non-ATPase regulatory subunit 11Add BLAST422

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei24Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ7KLV9.
PRIDEiQ7KLV9.

PTM databases

iPTMnetiQ7KLV9.

Expressioni

Developmental stagei

Highly expressed in the female germline, more precisely in the nurse cells of maturing egg chambers. Also detected in the somatic follicle cells. Highly expressed during oogenesis and early blastoderm embryos. After the beginning of zygotic transcription, present in the elongating germband and later in the mesodermal region and the developing hindgut and posterior midgut. In late embryos, shortly before hatching, becomes restricted to the brain hemispheres, the central nervous system, the embryonic gonads and the gut. In third instar larval tissues, expressed in the larval brain and in all imaginal disks.1 Publication

Gene expression databases

BgeeiFBgn0028689.
ExpressionAtlasiQ7KLV9. differential.
GenevisibleiQ7KLV9. DM.

Interactioni

Subunit structurei

Component of the lid subcomplex of the 19S proteasome regulatory particle complex (also named PA700 complex). The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. Interacts with alien/CSN2. Interacts with Prosalpha2 and Rpt6.2 Publications

Protein-protein interaction databases

BioGridi62384. 37 interactors.
DIPiDIP-60004N.
IntActiQ7KLV9. 8 interactors.
STRINGi7227.FBpp0086603.

Structurei

Secondary structure

1422
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi39 – 60Combined sources22
Helixi63 – 72Combined sources10
Turni73 – 75Combined sources3
Helixi76 – 79Combined sources4
Helixi82 – 96Combined sources15
Helixi103 – 119Combined sources17
Helixi123 – 139Combined sources17
Helixi143 – 157Combined sources15
Helixi164 – 179Combined sources16
Helixi183 – 199Combined sources17
Helixi204 – 220Combined sources17
Helixi225 – 239Combined sources15
Turni240 – 242Combined sources3
Helixi244 – 260Combined sources17
Helixi264 – 266Combined sources3
Helixi267 – 272Combined sources6
Helixi274 – 277Combined sources4
Helixi282 – 295Combined sources14
Helixi299 – 308Combined sources10
Turni310 – 315Combined sources6
Helixi317 – 339Combined sources23
Beta strandi343 – 346Combined sources4
Helixi347 – 354Combined sources8
Helixi358 – 370Combined sources13
Beta strandi376 – 379Combined sources4
Turni380 – 383Combined sources4
Beta strandi384 – 387Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TXMX-ray3.00A30-422[»]
3TXNX-ray2.50A30-422[»]
ProteinModelPortaliQ7KLV9.
SMRiQ7KLV9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini286 – 387PCIAdd BLAST102

Sequence similaritiesi

Belongs to the proteasome subunit S9 family.Curated

Phylogenomic databases

eggNOGiKOG1463. Eukaryota.
COG5159. LUCA.
GeneTreeiENSGT00530000063301.
InParanoidiQ7KLV9.
KOiK03036.
OMAiCKIMLGQ.
OrthoDBiEOG091G0B4C.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.25.40.10. 1 hit.
InterProiView protein in InterPro
IPR000717. PCI_dom.
IPR035295. PSMD11.
IPR011990. TPR-like_helical_dom.
IPR011991. WHTH_DNA-bd_dom.
PANTHERiPTHR10678:SF8. PTHR10678:SF8. 1 hit.
PfamiView protein in Pfam
PF01399. PCI. 1 hit.
SMARTiView protein in SMART
SM00088. PINT. 1 hit.
SUPFAMiSSF46785. SSF46785. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform B (identifier: Q7KLV9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGATLFERA QALSSVNREE QDSSLLNKLV RDQEGAENDE ERIRIKEQGI
60 70 80 90 100
LQQGELYKQE GKAKELADLI KVTRPFLSSI SKAKAAKLVR SLVDMFLDMD
110 120 130 140 150
AGTGIEVQLC KDCIEWAKQE KRTFLRQSLE ARLIALYFDT ALYTEALALG
160 170 180 190 200
AQLLRELKKL DDKNLLVEVQ LLESKTYHAL SNLPKARAAL TSARTTANAI
210 220 230 240 250
YCPPKVQGAL DLQSGILHAA DERDFKTAFS YFYEAFEGFD SVDSVKALTS
260 270 280 290 300
LKYMLLCKIM LGQSDDVNQL VSGKLAITYS GRDIDAMKSV AEASHKRSLA
310 320 330 340 350
DFQAALKEYK KELAEDVIVQ AHLGTLYDTM LEQNLCRIIE PYSRVQVAHV
360 370 380 390 400
AESIQLPMPQ VEKKLSQMIL DKKFSGILDQ GEGVLIVFEE TPVDKTYERV
410 420
LETIQSMGKV VDTLYQKAKK LS
Length:422
Mass (Da):47,263
Last modified:October 3, 2006 - v1
Checksum:i57F1D24C4303F8C5
GO
Isoform A (identifier: Q7KLV9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MTELLKENRSNEARRRKM

Show »
Length:439
Mass (Da):49,377
Checksum:i661930E9A5E603A7
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0444011M → MTELLKENRSNEARRRKM in isoform A. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF145309 mRNA. Translation: AAF08390.1.
AE013599 Genomic DNA. Translation: AAF58212.1.
AE013599 Genomic DNA. Translation: AAF58213.2.
AF160939 mRNA. Translation: AAD46879.1.
BT089015 mRNA. Translation: ACT98662.1.
RefSeqiNP_001246325.1. NM_001259396.2. [Q7KLV9-1]
NP_477474.1. NM_058126.5. [Q7KLV9-1]
NP_725412.2. NM_166065.3. [Q7KLV9-2]
UniGeneiDm.3085.

Genome annotation databases

EnsemblMetazoaiFBtr0087474; FBpp0086603; FBgn0028689. [Q7KLV9-2]
FBtr0087475; FBpp0086604; FBgn0028689. [Q7KLV9-1]
FBtr0306558; FBpp0297513; FBgn0028689. [Q7KLV9-1]
GeneIDi36638.
KEGGidme:Dmel_CG10149.
UCSCiCG10149-RA. d. melanogaster.
CG10149-RB. d. melanogaster. [Q7KLV9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPSD11_DROME
AccessioniPrimary (citable) accession number: Q7KLV9
Secondary accession number(s): A1Z9U3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2012
Last sequence update: October 3, 2006
Last modified: September 27, 2017
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families