Q7KLE5 (Q7KLE5_DROME) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Amphiphysin EMBL AAD46684.1

Submitted name:
LD19810p EMBL AAL28826.1

Gene names

Name:	Amph FlyBase FBgn0027356
Synonyms:	Amp EMBL AAL28826.1
ORF Names:	CG8604 FlyBase FBgn0027356, Dmel_CG8604 EMBL AAF58486.1

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome] EMBL AAD46684.1

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	602 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Sequence similarities

Contains 1 SH3 domain. RuleBase RU003338

Ontologies

Keywords
Domain	SH3 domain RuleBase RU003338
Technical term	3D-structure PDB 1URU Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	exocytosis Traceable author statement PubMed 14630214. Source: FlyBase neurotransmitter secretion Non-traceable author statement PubMed 10218159. Source: FlyBase protein localization Inferred from mutant phenotype PubMed 11748137. Source: FlyBase regulation of muscle contraction Inferred from mutant phenotype PubMed 11711432. Source: FlyBase rhabdomere membrane biogenesis Inferred from mutant phenotype PubMed 11748137. Source: FlyBase synaptic vesicle endocytosis Traceable author statement PubMed 10218159. Source: FlyBase
Cellular_component	cytoplasm Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from direct assay PubMed 11748137PubMed 20462449. Source: FlyBase synapse Inferred from direct assay PubMed 11733051PubMed 11748137. Source: FlyBase
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q7KLE5 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: 86D1D4CBAE1B8F31	FASTA	602	65,902
	10 20 30 40 50 60 MTENKGIMLA KSVQKHAGRA KEKILQNLGK VDRTADEIFD DHLNNFNRQQ ASANRLQKEF 70 80 90 100 110 120 NNYIRCVRAA QAASKTLMDS VCEIYEPQWS GYDALQAQTG ASESLWADFA HKLGDQVLIP 130 140 150 160 170 180 LNTYTGQFPE MKKKVEKRNR KLIDYDGQRH SFQNLQANAN KRKDDVKLTK GREQLEEARR 190 200 210 220 230 240 TYEILNTELH DELPALYDSR ILFLVTNLQT LFATEQVFHN ETAKIYSELE AIVDKLATES 250 260 270 280 290 300 QRGSNTLRKQ TSNPIKTSSP VQSPVNKLNN ANINSNYQNQ ITTNGGSSLA NSPTSTSSSL 310 320 330 340 350 360 QEPRFDSVSS TPEPRPESPA AALVSATPSS PVENGVTTKS LERPELSGLN ASAKATTTTQ 370 380 390 400 410 420 TSPTEDKAVV SEAVKPSETE GAAVAASVTP APPATPAQIN GNNNEPSIVK EGGKQPKELP 430 440 450 460 470 480 STTSNAEAAA EAAANNGNSI EEHKQKKLGN DTTVTATETV TVTQHSVTST DTDNIVTISD 490 500 510 520 530 540 TNTDTDTKTS TGTSQKGRPV PVVNRHSVNN LNKNPFEDDD ERIYEVPADA NTADLPPGVL 550 560 570 580 590 600 YRVKATYGYA KEDVDELSFE IGDLIRVIEY DDPEDQEEGW LMGQKEGTNE KGLFPANFTR PI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Dissecting the funtions of amphiphysin." Zhang Y.Q., Xue H., Broadie K.S. Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Characterisation of the gene for Drosophila amphiphysin." Razzaq A., Su Y., Mehren J.E., Mizuguchi K., Jackson A.P., Gay N.J., O'Kane C.J. Gene 241:167-174(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J., Champe M., Chavez C., Dorsett V., Farfan D., Frise E., George R., Gonzalez M., Guarin H., Li P., Liao G., Miranda A., Mungall C.J., Nunoo J. , Pacleb J., Paragas V., Park S., Phouanenavong S., Wan K., Yu C., Lewis S.E., Rubin G.M., Celniker S. Submitted (OCT-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: Berkeley EMBL AAL28826.1.
[5]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[6]	"BAR domains as sensors of membrane curvature: the amphiphysin BAR structure." Peter B.J., Kent H.M., Mills I.G., Vallis Y., Butler P.J., Evans P.R., McMahon H.T. Science 303:495-499(2004) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS) OF 1-244.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF171225 mRNA. Translation: AAD46684.1.
AE013599 Genomic DNA. Translation: AAF58486.1.
AY061278 mRNA. Translation: AAL28826.1.
AJ242855 mRNA. Translation: CAB45188.1.

RefSeq

NP_523717.1. NM_078993.3.

UniGene

Dm.3121.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1URU	X-ray	2.60	A	1-244	[»]

SMR

Q7KLE5. Positions 26-242, 534-602.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q7KLE5. 4 interactions.

STRING

7227.FBpp0086941.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblMetazoa

FBtr0087828; FBpp0086941; FBgn0027356.

GeneID

36383.

KEGG

dme:Dmel_CG8604.

UCSC

CG8604-RA. d. melanogaster.

Organism-specific databases

CTD

273.

FlyBase

FBgn0027356. Amph.

Phylogenomic databases

eggNOG

NOG264615.

GeneTree

ENSGT00390000017588.

InParanoid

Q7KLE5.

KO

K12562.

OMA

FQNLQAN.

OrthoDB

EOG498SHH.

Family and domain databases

Gene3D

1.20.1270.60. 1 hit.

InterPro

IPR027267. AH/BAR-dom.
IPR003005. Amphiphysin.
IPR004148. BAR_dom.
IPR001452. SH3_domain.
[Graphical view]

Pfam

PF03114. BAR. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]

PRINTS

PR01251. AMPHIPHYSIN.

SMART

SM00721. BAR. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]

SUPFAM

SSF50044. SH3. 1 hit.

PROSITE

PS51021. BAR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

Amph. drosophila.

EvolutionaryTrace

Q7KLE5.

GenomeRNAi

36383.

NextBio

798246.

Entry information

Entry name

Q7KLE5_DROME

Accession

Primary (citable) accession number: Q7KLE5

Entry history

Integrated into UniProtKB/TrEMBL:	October 3, 2006
Last sequence update:	October 3, 2006
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)