Reviewed, UniProtKB/Swiss-Prot Q7KHA1 (PGM_DROSI)

Names and origin

Protein names

Recommended name:
    Phosphoglucomutase
      Short name=PGM
    EC=5.4.2.2
Alternative name(s):
    Glucose phosphomutase

Gene names

Name:

Pgm

Organism

Drosophila simulans (Fruit fly)

Taxonomic identifier

7240 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	560 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This enzyme participates in both the breakdown and synthesis of glucose By similarity. UniProtKB P00949
Catalytic activity	Alpha-D-glucose 1-phosphate = alpha-D-glucose 6-phosphate. UniProtKB P00949
Cofactor	Binds 1 magnesium ion per subunit By similarity. UniProtKB P00949
Sequence similarities	Belongs to the phosphohexose mutase family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glucose metabolism
Coding sequence diversity	Polymorphism
Ligand	Magnesium Metal-binding
Molecular function	Isomerase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglucomutase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 560	560	Phosphoglucomutase		PRO_0000147790
Sites
	Active site	116	1	Phosphoserine intermediate By similarity UniProtKB P00949
	Metal binding	116	1	Magnesium; via phosphate group By similarity UniProtKB P00949
	Metal binding	288	1	Magnesium By similarity UniProtKB P00949
	Metal binding	290	1	Magnesium By similarity UniProtKB P00949
	Metal binding	292	1	Magnesium By similarity UniProtKB P00949
Natural variations
	Natural variant	45	1	E → A in strain: dpf96_10.6. Ref.1
	Natural variant	49	1	A → G in strain: dpf96_10.6. Ref.1
	Natural variant	157	1	K → M in strain: dpf96_36.2. Ref.1
	Natural variant	175	1	D → N in strain: dpf96_17.4. Ref.1
	Natural variant	450	1	T → I in strain: dpf96_36.2. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q7KHA1-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 10320D78D2CA2CEC	FASTA	560	60,794
	10 20 30 40 50 60 MSLTVEIVAT KPYEGQKPGT SGLRKKVKVF TQPNYTENFV QAILEANGAA LVGSTLVVGG 70 80 90 100 110 120 DGRFYCKEAA ELIVRLSAAN GVSKLLVGQN GILSTPAVSS LIRHNKALGG IVLTASHNPG 130 140 150 160 170 180 GPENDFGIKF NCENGGPAPD AFTNHIYKIT TEIKEYKLVR NLQIDISKVG VTSFDIAGKP 190 200 210 220 230 240 FTVEVIDSVA NYVRHMEEIF DFAKLKDFVS GKATGKPLKM RIDAMNGVTG SYVREIFLNR 250 260 270 280 290 300 LGATESSVVH TTPLPDFGGL HPDPNLTYAK DLVDTVAQGD YDIGAAFDGD GDRNMIIGSK 310 320 330 340 350 360 AFFVTPSDSL AVIAHYLEAI PYFQKNGVQG FARSMPTASA VDLVGRKLGK EVFEVPTGWK 370 380 390 400 410 420 YFGNLMDAGR LCLCGEESFG TGSNHIREKD GIWAVLAWIS VMQHTGKGIE DILKQHWSVY 430 440 450 460 470 480 GRNYFTRYDY EECASDPCNE MVATMEKTIT APEFVGKSYS SGGKTYKVKE ADNFSYTDPV 490 500 510 520 530 540 DKSVATKQGL RIVFEDGSRI VVRLSGTGSS GATVRLYIDS YEKENVLGQA SVMLKPLIDI 550 560 ALEISQLPKF TGRNAPTVIT

References

[1]

"Extensive amino acid polymorphism at the pgm locus is consistent with adaptive protein evolution in Drosophila melanogaster." Verrelli B.C., Eanes W.F. Genetics 156:1737-1752(2000) [PubMed: 11102370] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ALA-45; GLY-49; MET-157; ASN-175 AND ILE-450. Strain: dpf96_10.6, dpf96_13.1, dpf96_13.2, dpf96_17.4, dpf96_18.0, dpf96_20.3, dpf96_22.2, dpf96_3.0, dpf96_36.2, dpf96_43.3, dpf96_5.1, dpf96_7.0 and dpf96_9.4.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF290357 Genomic DNA. Translation: AAG42290.1. AF290358 Genomic DNA. Translation: AAG42291.1. AF290359 Genomic DNA. Translation: AAG42292.1. AF290360 Genomic DNA. Translation: AAG42293.1. AF290361 Genomic DNA. Translation: AAG42294.1. AF290362 Genomic DNA. Translation: AAG42295.1. AF290363 Genomic DNA. Translation: AAG42296.1. AF290364 Genomic DNA. Translation: AAG42297.1. AF290365 Genomic DNA. Translation: AAG42298.1. AF290366 Genomic DNA. Translation: AAG42299.1. AF290367 Genomic DNA. Translation: AAG42300.1. AF290368 Genomic DNA. Translation: AAG42301.1. AF290369 Genomic DNA. Translation: AAG42302.1.
3D structure databases
HSSP	HSSP built from PDB template 1KFI based on UniProtKB P47244.
ModBase	Search...
Organism-specific databases
FlyBase	FBgn0020699. Dsim\Pgm.
Enzyme and pathway databases
BRENDA	5.4.2.2. 189309.
Family and domain databases
InterPro	IPR005844. A-D-PHexomutase_a/b/a-I. IPR016055. A-D-PHexomutase_a/b/a-I/II/III. IPR005845. A-D-PHexomutase_a/b/a-II. IPR005846. A-D-PHexomutase_a/b/a-III. IPR005843. A-D-PHexomutase_C. IPR016066. A-D-PHexomutase_CS. IPR005841. A-D-PHexomutase_N. [Graphical view]
Gene3D	G3DSA:3.40.120.10. A-D-PHexomutase_a/b/a-I/II/III. 3 hits.
Pfam	PF02878. PGM_PMM_I. 1 hit. PF02879. PGM_PMM_II. 1 hit. PF02880. PGM_PMM_III. 1 hit. PF00408. PGM_PMM_IV. 1 hit. [Graphical view]
PRINTS	PR00509. PGMPMM.
PROSITE	PS00710. PGM_PMM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGM_DROSI

Accession

Primary (citable) accession number: Q7KHA1
Secondary accession number(s): Q9GQ68, Q9GQ69, Q9GQ70

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
Last sequence update:	July 5, 2004
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project