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Protein

Flap endonuclease 1

Gene

Fen1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi34 – 341Magnesium 1UniRule annotation
Binding sitei47 – 471DNA substrateUniRule annotation
Binding sitei70 – 701DNA substrateUniRule annotation
Metal bindingi86 – 861Magnesium 1UniRule annotation
Metal bindingi158 – 1581Magnesium 1UniRule annotation
Binding sitei158 – 1581DNA substrateUniRule annotation
Metal bindingi160 – 1601Magnesium 1UniRule annotation
Metal bindingi179 – 1791Magnesium 2UniRule annotation
Metal bindingi181 – 1811Magnesium 2UniRule annotation
Binding sitei231 – 2311DNA substrateUniRule annotation
Metal bindingi233 – 2331Magnesium 2UniRule annotation
Binding sitei233 – 2331DNA substrateUniRule annotation

GO - Molecular functioni

  • 5'-3' exonuclease activity Source: UniProtKB-HAMAP
  • 5'-flap endonuclease activity Source: UniProtKB-HAMAP
  • DNA binding Source: UniProtKB-HAMAP
  • endonuclease activity Source: FlyBase
  • magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  • base-excision repair Source: UniProtKB-HAMAP
  • DNA replication, removal of RNA primer Source: UniProtKB-HAMAP
  • neurogenesis Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Exonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair, DNA replication

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Flap endonuclease 1UniRule annotation (EC:3.1.-.-UniRule annotation)
Short name:
FEN-1UniRule annotation
Alternative name(s):
Flap structure-specific endonuclease 1UniRule annotation
Gene namesi
Name:Fen1UniRule annotation
ORF Names:CG8648
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0025832. Fen1.

Subcellular locationi

  • Nucleusnucleolus UniRule annotation
  • Nucleusnucleoplasm UniRule annotation
  • Mitochondrion UniRule annotation

  • Note: Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 385385Flap endonuclease 1PRO_0000403500Add
BLAST

Post-translational modificationi

Phosphorylated. Phosphorylation upon DNA damage induces relocalization to the nuclear plasma.UniRule annotation

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ7K7A9.
PRIDEiQ7K7A9.

Expressioni

Gene expression databases

BgeeiFBgn0025832.
GenevisibleiQ7K7A9. DM.

Interactioni

Subunit structurei

Interacts with PCNA. Three molecules of FEN1 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity.UniRule annotation

Protein-protein interaction databases

BioGridi62595. 1 interaction.
IntActiQ7K7A9. 2 interactions.
STRINGi7227.FBpp0086223.

Structurei

3D structure databases

ProteinModelPortaliQ7K7A9.
SMRiQ7K7A9. Positions 2-336.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 104104N-domainAdd
BLAST
Regioni122 – 253132I-domainAdd
BLAST
Regioni336 – 3449Interaction with PCNAUniRule annotation

Sequence similaritiesi

Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG2519. Eukaryota.
COG0258. LUCA.
GeneTreeiENSGT00640000091478.
InParanoidiQ7K7A9.
KOiK04799.
OMAiLCGDRQF.
OrthoDBiEOG091G0C0E.
PhylomeDBiQ7K7A9.

Family and domain databases

CDDicd09867. PIN_FEN1. 1 hit.
Gene3Di3.40.50.1010. 1 hit.
HAMAPiMF_00614. Fen. 1 hit.
InterProiIPR020045. 5-3_exonuclease_C.
IPR023426. Flap_endonuc.
IPR008918. HhH2.
IPR029060. PIN_domain-like.
IPR006086. XPG-I_dom.
IPR006084. XPG/Rad2.
IPR019974. XPG_CS.
IPR006085. XPG_DNA_repair_N.
[Graphical view]
PANTHERiPTHR11081. PTHR11081. 1 hit.
PfamiPF00867. XPG_I. 1 hit.
PF00752. XPG_N. 1 hit.
[Graphical view]
PRINTSiPR00853. XPGRADSUPER.
SMARTiSM00279. HhH2. 1 hit.
SM00484. XPGI. 1 hit.
SM00485. XPGN. 1 hit.
[Graphical view]
SUPFAMiSSF47807. SSF47807. 1 hit.
SSF88723. SSF88723. 1 hit.
PROSITEiPS00841. XPG_1. 1 hit.
PS00842. XPG_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7K7A9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGILGLSKLI ADLAPQAIRE SEMKHFFGRK VAIDASMCLY QFLIAVRSEG
60 70 80 90 100
AQLATVNGDP TSHLMGMFYR TIRLLDNGIK PVYVFDGKPP DLKSGELAKR
110 120 130 140 150
AERREEAEKA LKAATDAGDD AGIEKFNRRL VRVTKEHAKE AKELLTLMGV
160 170 180 190 200
PYVDAPCEAE AQCAALVKAG KVYATATEDM DALTFGSTKL LRYLTYSEAR
210 220 230 240 250
KMPVKEFSYD KLLEGLAINN REFIDLCILL GCDYCESIKG IGPKRAIELI
260 270 280 290 300
NTYRDIETIL DNLDSSKYTV PENWNYKVAR ELFIEPEVAD ADSIDLKWVE
310 320 330 340 350
PDEEGLVKFL CGDRQFNEER VRNGAKKLMK SKQAQTQVRL DSFFKTLPST
360 370 380
PNATNAAKRK AEEAKKSANN KKAKTSGGGR GRRPK
Length:385
Mass (Da):42,948
Last modified:October 3, 2006 - v1
Checksum:iD0A0831C2BDA9240
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013599 Genomic DNA. Translation: AAF57944.1.
AL031863 Genomic DNA. Translation: CAA21320.1.
BT100031 mRNA. Translation: ACX54939.1.
PIRiT13692.
RefSeqiNP_523765.1. NM_079041.2.
UniGeneiDm.23253.

Genome annotation databases

EnsemblMetazoaiFBtr0087075; FBpp0086223; FBgn0025832.
GeneIDi36887.
KEGGidme:Dmel_CG8648.
UCSCiCG8648-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE013599 Genomic DNA. Translation: AAF57944.1.
AL031863 Genomic DNA. Translation: CAA21320.1.
BT100031 mRNA. Translation: ACX54939.1.
PIRiT13692.
RefSeqiNP_523765.1. NM_079041.2.
UniGeneiDm.23253.

3D structure databases

ProteinModelPortaliQ7K7A9.
SMRiQ7K7A9. Positions 2-336.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi62595. 1 interaction.
IntActiQ7K7A9. 2 interactions.
STRINGi7227.FBpp0086223.

Proteomic databases

PaxDbiQ7K7A9.
PRIDEiQ7K7A9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0087075; FBpp0086223; FBgn0025832.
GeneIDi36887.
KEGGidme:Dmel_CG8648.
UCSCiCG8648-RA. d. melanogaster.

Organism-specific databases

CTDi2237.
FlyBaseiFBgn0025832. Fen1.

Phylogenomic databases

eggNOGiKOG2519. Eukaryota.
COG0258. LUCA.
GeneTreeiENSGT00640000091478.
InParanoidiQ7K7A9.
KOiK04799.
OMAiLCGDRQF.
OrthoDBiEOG091G0C0E.
PhylomeDBiQ7K7A9.

Miscellaneous databases

GenomeRNAii36887.
PROiQ7K7A9.

Gene expression databases

BgeeiFBgn0025832.
GenevisibleiQ7K7A9. DM.

Family and domain databases

CDDicd09867. PIN_FEN1. 1 hit.
Gene3Di3.40.50.1010. 1 hit.
HAMAPiMF_00614. Fen. 1 hit.
InterProiIPR020045. 5-3_exonuclease_C.
IPR023426. Flap_endonuc.
IPR008918. HhH2.
IPR029060. PIN_domain-like.
IPR006086. XPG-I_dom.
IPR006084. XPG/Rad2.
IPR019974. XPG_CS.
IPR006085. XPG_DNA_repair_N.
[Graphical view]
PANTHERiPTHR11081. PTHR11081. 1 hit.
PfamiPF00867. XPG_I. 1 hit.
PF00752. XPG_N. 1 hit.
[Graphical view]
PRINTSiPR00853. XPGRADSUPER.
SMARTiSM00279. HhH2. 1 hit.
SM00484. XPGI. 1 hit.
SM00485. XPGN. 1 hit.
[Graphical view]
SUPFAMiSSF47807. SSF47807. 1 hit.
SSF88723. SSF88723. 1 hit.
PROSITEiPS00841. XPG_1. 1 hit.
PS00842. XPG_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFEN1_DROME
AccessioniPrimary (citable) accession number: Q7K7A9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: October 3, 2006
Last modified: September 7, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.