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Q7K755 (GLT11_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative polypeptide N-acetylgalactosaminyltransferase 11

Short name=pp-GaNTase 11
EC=2.4.1.41
Alternative name(s):
Protein-UDP acetylgalactosaminyltransferase 11
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 11
Gene names
Name:gly-11
ORF Names:Y75B8A.9
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length605 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

May catalyze the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor By similarity.

Catalytic activity

UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide.

Cofactor

Manganese By similarity.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein By similarity.

Domain

There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding By similarity.

The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity By similarity.

Sequence similarities

Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.

Contains 1 ricin B-type lectin domain.

Ontologies

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform a (identifier: Q7K755-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform b (identifier: Q7K755-2)

The sequence of this isoform differs from the canonical sequence as follows:
     436-436: G → V
     437-605: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 605605Putative polypeptide N-acetylgalactosaminyltransferase 11
PRO_0000059154

Regions

Topological domain1 – 1111Cytoplasmic Potential
Transmembrane12 – 2918Helical; Signal-anchor for type II membrane protein; Potential
Topological domain30 – 605576Lumenal Potential
Domain481 – 605125Ricin B-type lectin
Region158 – 271114Catalytic subdomain A
Region329 – 39163Catalytic subdomain B

Sites

Metal binding2551Manganese By similarity
Metal binding2571Manganese By similarity
Metal binding3881Manganese By similarity
Binding site1991Substrate By similarity
Binding site2561Substrate By similarity
Binding site3601Substrate By similarity
Binding site3911Substrate By similarity
Binding site3961Substrate By similarity

Amino acid modifications

Glycosylation2051N-linked (GlcNAc...) Potential
Glycosylation4901N-linked (GlcNAc...) Potential
Disulfide bond149 ↔ 383 By similarity
Disulfide bond374 ↔ 450 By similarity
Disulfide bond494 ↔ 515 By similarity
Disulfide bond541 ↔ 553 By similarity
Disulfide bond578 ↔ 594 By similarity

Natural variations

Alternative sequence4361G → V in isoform b.
VSP_011242
Alternative sequence437 – 605169Missing in isoform b.
VSP_011243

Sequences

Sequence LengthMass (Da)Tools
Isoform a [UniParc].

Last modified August 16, 2004. Version 2.
Checksum: F84C132FA794C661

FASTA60569,597
        10         20         30         40         50         60 
MLLLWRRKTV GLICLFSILL TIYLYVSMYP EDNERVWNSG NADIQRKHEK LNNGGQGRHD 

        70         80         90        100        110        120 
FDDDEGAEKD EEDAVEKQNI AAPPLPKSFT TFPDRSKEIE IDTDLLGKIN GKAEDDLQVE 

       130        140        150        160        170        180 
GYKKYQFNGL LSDRIGSRRK IKDSRNARCS SLTYSDSLPA ASIVVCYFNE SPSVLIRMVN 

       190        200        210        220        230        240 
SIFDRTKPEH LHEILLVDDS SEWSNATDEA IKYREKHIIQ WEKVKFLKTD KNEGLIRAKI 

       250        260        270        280        290        300 
FGARRANGEV LVFLDSHCEV NEEWLPPLLD QIKQNRRRVV CPIIDIIDAI TMKYVESPVC 

       310        320        330        340        350        360 
TGGVNWAMTF KWDYPHRSYF EDPMNYVNPL KSPTMAGGLF AIDKEYFFEI GSYDEGMDVW 

       370        380        390        400        410        420 
GAENVEISVR IWTCGGELLI MPCSRVGHIF RRQRPYGIKT DSMGKNSVRL ARVWLDEYLE 

       430        440        450        460        470        480 
NFFEARPNYR TFTDYGDLTS RISLRRNLQC KPFKWYLENI YPELLPDNTP NQLNNQILVA 

       490        500        510        520        530        540 
GKKYLIKMAN GTHCLSAENS QGRIANGNRV EMRKCNHMER MQQWKYSSTN ELRPMGSSRM 

       550        560        570        580        590        600 
CLDSLRGISV ILCHNQGAHQ MWQVSNAGKL YSRSVNKCAT GSNDVSALST LKFCSLANSF 


QFVEL 

« Hide

Isoform b [UniParc].

Checksum: 16BC07CEE870C051
Show »

FASTA43650,453

References

[1]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL033514 Genomic DNA. Translation: CAA22098.1.
AL033514 Genomic DNA. Translation: CAE47472.1.
PIRT27397.
RefSeqNP_001022948.1. NM_001027777.3. [Q7K755-1]
NP_001022949.1. NM_001027778.3. [Q7K755-2]
UniGeneCel.62.

3D structure databases

ProteinModelPortalQ7K755.
SMRQ7K755. Positions 122-602.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING6239.Y75B8A.9a.

Protein family/group databases

CAZyGT27. Glycosyltransferase Family 27.

Proteomic databases

PaxDbQ7K755.
PRIDEQ7K755.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaY75B8A.9a; Y75B8A.9a; WBGene00001636. [Q7K755-1]
GeneID176647.
KEGGcel:CELE_Y75B8A.9.
UCSCY75B8A.9a. c. elegans. [Q7K755-1]

Organism-specific databases

CTD176647.
WormBaseY75B8A.9a; CE23021; WBGene00001636; gly-11.
Y75B8A.9b; CE35810; WBGene00001636; gly-11.

Phylogenomic databases

eggNOGNOG239675.
GeneTreeENSGT00740000115054.
HOGENOMHOG000038227.
InParanoidQ7K755.
OMAANGTHCL.
OrthoDBEOG7J9VP2.
PhylomeDBQ7K755.

Enzyme and pathway databases

UniPathwayUPA00378.

Family and domain databases

Gene3D3.90.550.10. 1 hit.
InterProIPR027791. Galactosyl_T_C.
IPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
IPR000772. Ricin_B_lectin.
[Graphical view]
PfamPF02709. Glyco_transf_7C. 1 hit.
PF00535. Glycos_transf_2. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
[Graphical view]
SMARTSM00458. RICIN. 1 hit.
[Graphical view]
SUPFAMSSF50370. SSF50370. 1 hit.
SSF53448. SSF53448. 1 hit.
PROSITEPS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio893444.
PROQ7K755.

Entry information

Entry nameGLT11_CAEEL
AccessionPrimary (citable) accession number: Q7K755
Secondary accession number(s): Q9XW72
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: July 9, 2014
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase