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Protein

Putative polypeptide N-acetylgalactosaminyltransferase 11

Gene

gly-11

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

May catalyze the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor.By similarity

Catalytic activityi

UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide.

Cofactori

Mn2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei199SubstrateBy similarity1
Metal bindingi255ManganeseBy similarity1
Binding sitei256SubstrateBy similarity1
Metal bindingi257ManganeseBy similarity1
Binding sitei360SubstrateBy similarity1
Metal bindingi388ManganeseBy similarity1
Binding sitei391SubstrateBy similarity1
Binding sitei396SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
LigandLectin, Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-CEL-913709 O-linked glycosylation of mucins
UniPathwayiUPA00378

Protein family/group databases

CAZyiCBM13 Carbohydrate-Binding Module Family 13
GT27 Glycosyltransferase Family 27

Names & Taxonomyi

Protein namesi
Recommended name:
Putative polypeptide N-acetylgalactosaminyltransferase 11 (EC:2.4.1.41)
Short name:
pp-GaNTase 11
Alternative name(s):
Protein-UDP acetylgalactosaminyltransferase 11
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 11
Gene namesi
Name:gly-11
ORF Names:Y75B8A.9
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiY75B8A.9a ; CE23021 ; WBGene00001636 ; gly-11
Y75B8A.9b ; CE35810 ; WBGene00001636 ; gly-11

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 11CytoplasmicSequence analysisAdd BLAST11
Transmembranei12 – 29Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST18
Topological domaini30 – 605LumenalSequence analysisAdd BLAST576

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000591541 – 605Putative polypeptide N-acetylgalactosaminyltransferase 11Add BLAST605

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi149 ↔ 383PROSITE-ProRule annotation
Glycosylationi205N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi374 ↔ 450PROSITE-ProRule annotation
Glycosylationi490N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi494 ↔ 515PROSITE-ProRule annotation
Disulfide bondi541 ↔ 553PROSITE-ProRule annotation
Disulfide bondi578 ↔ 594PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ7K755
PaxDbiQ7K755
PeptideAtlasiQ7K755
PRIDEiQ7K755

PTM databases

iPTMnetiQ7K755

Expressioni

Gene expression databases

BgeeiWBGene00001636

Interactioni

Protein-protein interaction databases

STRINGi6239.Y75B8A.9a

Structurei

3D structure databases

ProteinModelPortaliQ7K755
SMRiQ7K755
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini481 – 605Ricin B-type lectinPROSITE-ProRule annotationAdd BLAST125

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni158 – 271Catalytic subdomain AAdd BLAST114
Regioni329 – 391Catalytic subdomain BAdd BLAST63

Domaini

There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.By similarity
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.By similarity

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3736 Eukaryota
ENOG410XPMK LUCA
GeneTreeiENSGT00760000118828
HOGENOMiHOG000038227
InParanoidiQ7K755
KOiK00710
OMAiVILCHNQ
OrthoDBiEOG091G085O
PhylomeDBiQ7K755

Family and domain databases

CDDicd00161 RICIN, 1 hit
Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR027791 Galactosyl_T_C
IPR001173 Glyco_trans_2-like
IPR029044 Nucleotide-diphossugar_trans
IPR035992 Ricin_B-like_lectins
IPR000772 Ricin_B_lectin
PfamiView protein in Pfam
PF02709 Glyco_transf_7C, 1 hit
PF00535 Glycos_transf_2, 1 hit
PF00652 Ricin_B_lectin, 1 hit
SMARTiView protein in SMART
SM00458 RICIN, 1 hit
SUPFAMiSSF50370 SSF50370, 1 hit
SSF53448 SSF53448, 1 hit
PROSITEiView protein in PROSITE
PS50231 RICIN_B_LECTIN, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: Q7K755-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLLLWRRKTV GLICLFSILL TIYLYVSMYP EDNERVWNSG NADIQRKHEK
60 70 80 90 100
LNNGGQGRHD FDDDEGAEKD EEDAVEKQNI AAPPLPKSFT TFPDRSKEIE
110 120 130 140 150
IDTDLLGKIN GKAEDDLQVE GYKKYQFNGL LSDRIGSRRK IKDSRNARCS
160 170 180 190 200
SLTYSDSLPA ASIVVCYFNE SPSVLIRMVN SIFDRTKPEH LHEILLVDDS
210 220 230 240 250
SEWSNATDEA IKYREKHIIQ WEKVKFLKTD KNEGLIRAKI FGARRANGEV
260 270 280 290 300
LVFLDSHCEV NEEWLPPLLD QIKQNRRRVV CPIIDIIDAI TMKYVESPVC
310 320 330 340 350
TGGVNWAMTF KWDYPHRSYF EDPMNYVNPL KSPTMAGGLF AIDKEYFFEI
360 370 380 390 400
GSYDEGMDVW GAENVEISVR IWTCGGELLI MPCSRVGHIF RRQRPYGIKT
410 420 430 440 450
DSMGKNSVRL ARVWLDEYLE NFFEARPNYR TFTDYGDLTS RISLRRNLQC
460 470 480 490 500
KPFKWYLENI YPELLPDNTP NQLNNQILVA GKKYLIKMAN GTHCLSAENS
510 520 530 540 550
QGRIANGNRV EMRKCNHMER MQQWKYSSTN ELRPMGSSRM CLDSLRGISV
560 570 580 590 600
ILCHNQGAHQ MWQVSNAGKL YSRSVNKCAT GSNDVSALST LKFCSLANSF

QFVEL
Note: No experimental confirmation available.
Length:605
Mass (Da):69,597
Last modified:August 16, 2004 - v2
Checksum:iF84C132FA794C661
GO
Isoform b (identifier: Q7K755-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     436-436: G → V
     437-605: Missing.

Note: No experimental confirmation available.
Show »
Length:436
Mass (Da):50,453
Checksum:i16BC07CEE870C051
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_011242436G → V in isoform b. Curated1
Alternative sequenceiVSP_011243437 – 605Missing in isoform b. CuratedAdd BLAST169

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL033514 Genomic DNA Translation: CAA22098.1
AL033514 Genomic DNA Translation: CAE47472.1
PIRiT27397
RefSeqiNP_001022948.1, NM_001027777.3 [Q7K755-1]
NP_001022949.1, NM_001027778.3 [Q7K755-2]
UniGeneiCel.62

Genome annotation databases

EnsemblMetazoaiY75B8A.9a; Y75B8A.9a; WBGene00001636 [Q7K755-1]
GeneIDi176647
KEGGicel:CELE_Y75B8A.9
UCSCiY75B8A.9a c. elegans [Q7K755-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGLT11_CAEEL
AccessioniPrimary (citable) accession number: Q7K755
Secondary accession number(s): Q9XW72
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: August 16, 2004
Last modified: April 25, 2018
This is version 112 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health