Q7K755 (GLT11_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative polypeptide N-acetylgalactosaminyltransferase 11
Short name=pp-GaNTase 11
EC=2.4.1.41

Alternative name(s):
Protein-UDP acetylgalactosaminyltransferase 11
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 11

Gene names

Name:	gly-11
ORF Names:	Y75B8A.9

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	605 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May catalyze the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor By similarity.
Catalytic activity	UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide.
Cofactor	Manganese By similarity. Calcium By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Domain	There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding By similarity. The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity By similarity.
Sequence similarities	Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily. Contains 1 ricin B-type lectin domain.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Calcium Lectin Manganese
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein glycosylation Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	polypeptide N-acetylgalactosaminyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform a (identifier: Q7K755-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform b (identifier: Q7K755-2)

The sequence of this isoform differs from the canonical sequence as follows:
     436-436: G → V
     437-605: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 605	605	Putative polypeptide N-acetylgalactosaminyltransferase 11		PRO_0000059154
Regions
	Topological domain	1 – 11	11	Cytoplasmic Potential
	Transmembrane	12 – 29	18	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	30 – 605	576	Lumenal Potential
	Domain	481 – 605	125	Ricin B-type lectin
	Region	158 – 271	114	Catalytic subdomain A
	Region	329 – 391	63	Catalytic subdomain B
Amino acid modifications
	Glycosylation	205	1	N-linked (GlcNAc...) Potential
	Glycosylation	490	1	N-linked (GlcNAc...) Potential
	Disulfide bond	494 ↔ 515		By similarity
	Disulfide bond	541 ↔ 553		By similarity
	Disulfide bond	578 ↔ 594		By similarity
Natural variations
	Alternative sequence	436	1	G → V in isoform b.		VSP_011242
	Alternative sequence	437 – 605	169	Missing in isoform b.		VSP_011243

Sequences

	Sequence		Length	Mass (Da)
	Isoform a [UniParc]. Last modified August 16, 2004. Version 2. Checksum: F84C132FA794C661	FASTA	605	69,597
	10 20 30 40 50 60 MLLLWRRKTV GLICLFSILL TIYLYVSMYP EDNERVWNSG NADIQRKHEK LNNGGQGRHD 70 80 90 100 110 120 FDDDEGAEKD EEDAVEKQNI AAPPLPKSFT TFPDRSKEIE IDTDLLGKIN GKAEDDLQVE 130 140 150 160 170 180 GYKKYQFNGL LSDRIGSRRK IKDSRNARCS SLTYSDSLPA ASIVVCYFNE SPSVLIRMVN 190 200 210 220 230 240 SIFDRTKPEH LHEILLVDDS SEWSNATDEA IKYREKHIIQ WEKVKFLKTD KNEGLIRAKI 250 260 270 280 290 300 FGARRANGEV LVFLDSHCEV NEEWLPPLLD QIKQNRRRVV CPIIDIIDAI TMKYVESPVC 310 320 330 340 350 360 TGGVNWAMTF KWDYPHRSYF EDPMNYVNPL KSPTMAGGLF AIDKEYFFEI GSYDEGMDVW 370 380 390 400 410 420 GAENVEISVR IWTCGGELLI MPCSRVGHIF RRQRPYGIKT DSMGKNSVRL ARVWLDEYLE 430 440 450 460 470 480 NFFEARPNYR TFTDYGDLTS RISLRRNLQC KPFKWYLENI YPELLPDNTP NQLNNQILVA 490 500 510 520 530 540 GKKYLIKMAN GTHCLSAENS QGRIANGNRV EMRKCNHMER MQQWKYSSTN ELRPMGSSRM 550 560 570 580 590 600 CLDSLRGISV ILCHNQGAHQ MWQVSNAGKL YSRSVNKCAT GSNDVSALST LKFCSLANSF QFVEL
	Isoform b [UniParc]. Checksum: 16BC07CEE870C051	FASTA	436	50,453
	10 20 30 40 50 60 MLLLWRRKTV GLICLFSILL TIYLYVSMYP EDNERVWNSG NADIQRKHEK LNNGGQGRHD 70 80 90 100 110 120 FDDDEGAEKD EEDAVEKQNI AAPPLPKSFT TFPDRSKEIE IDTDLLGKIN GKAEDDLQVE 130 140 150 160 170 180 GYKKYQFNGL LSDRIGSRRK IKDSRNARCS SLTYSDSLPA ASIVVCYFNE SPSVLIRMVN 190 200 210 220 230 240 SIFDRTKPEH LHEILLVDDS SEWSNATDEA IKYREKHIIQ WEKVKFLKTD KNEGLIRAKI 250 260 270 280 290 300 FGARRANGEV LVFLDSHCEV NEEWLPPLLD QIKQNRRRVV CPIIDIIDAI TMKYVESPVC 310 320 330 340 350 360 TGGVNWAMTF KWDYPHRSYF EDPMNYVNPL KSPTMAGGLF AIDKEYFFEI GSYDEGMDVW 370 380 390 400 410 420 GAENVEISVR IWTCGGELLI MPCSRVGHIF RRQRPYGIKT DSMGKNSVRL ARVWLDEYLE 430 NFFEARPNYR TFTDYV

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL033514 Genomic DNA. Translation: CAA22098.1. AL033514 Genomic DNA. Translation: CAE47472.1.
PIR	T27397.
RefSeq	NP_001022948.1. NM_001027777.3. NP_001022949.1. NM_001027778.3.
3D structure databases
ProteinModelPortal	Q7K755.
SMR	Q7K755. Positions 122-602.
ModBase	Search...
Protein-protein interaction databases
STRING	6239.Y75B8A.9a.
Protein family/group databases
CAZy	GT27. Glycosyltransferase Family 27.
Proteomic databases
PaxDb	Q7K755.
PRIDE	Q7K755.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	Y75B8A.9a; Y75B8A.9a; Y75B8A.9.
GeneID	176647.
KEGG	cel:CELE_Y75B8A.9.
UCSC	Y75B8A.9a. c. elegans.
Organism-specific databases
CTD	176647.
WormBase	Y75B8A.9a; CE23021; WBGene00001636; gly-11. Y75B8A.9b; CE35810; WBGene00001636; gly-11.
Phylogenomic databases
eggNOG	NOG239675.
GeneTree	ENSGT00700000104275.
HOGENOM	HOG000038227.
InParanoid	Q7K755.
OMA	ANGTHCL.
Enzyme and pathway databases
UniPathway	UPA00378.
Family and domain databases
InterPro	IPR003859. Galactosyl_T_2_met. IPR001173. Glyco_trans_2. IPR000772. Ricin_B_lectin. [Graphical view]
Pfam	PF02709. Glyco_transf_7C. 1 hit. PF00535. Glycos_transf_2. 1 hit. PF00652. Ricin_B_lectin. 1 hit. [Graphical view]
SMART	SM00458. RICIN. 1 hit. [Graphical view]
SUPFAM	SSF50370. RicinB_like. 1 hit.
PROSITE	PS50231. RICIN_B_LECTIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	893444.

Entry information

Entry name

GLT11_CAEEL

Accession

Primary (citable) accession number: Q7K755
Secondary accession number(s): Q9XW72

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	August 16, 2004
Last modified:	May 1, 2013
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project