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Protein

Flap endonuclease 1

Gene

FEN1

Organism
Plasmodium falciparum (isolate 3D7)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi34Magnesium 1UniRule annotation1
Binding sitei47DNA substrateUniRule annotation1
Binding sitei72DNA substrateUniRule annotation1
Metal bindingi88Magnesium 1UniRule annotation1
Metal bindingi160Magnesium 1UniRule annotation1
Binding sitei160DNA substrateUniRule annotation1
Metal bindingi162Magnesium 1UniRule annotation1
Metal bindingi181Magnesium 2UniRule annotation1
Metal bindingi183Magnesium 2UniRule annotation1
Binding sitei244DNA substrateUniRule annotation1
Metal bindingi246Magnesium 2UniRule annotation1
Binding sitei246DNA substrateUniRule annotation1

GO - Molecular functioni

  • DNA binding Source: InterPro
  • endodeoxyribonuclease activity Source: GeneDB
  • exonuclease activity Source: UniProtKB-KW
  • flap endonuclease activity Source: GeneDB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Exonuclease, Hydrolase, Nuclease
Biological processDNA damage, DNA repair, DNA replication
LigandMagnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-PFA-69166. Removal of the Flap Intermediate.

Names & Taxonomyi

Protein namesi
Recommended name:
Flap endonuclease 1UniRule annotation (EC:3.1.-.-UniRule annotation)
Short name:
FEN-1UniRule annotation
Alternative name(s):
Flap structure-specific endonuclease 1UniRule annotation
Gene namesi
Name:FEN1UniRule annotation
ORF Names:PFD0420c
OrganismiPlasmodium falciparum (isolate 3D7)
Taxonomic identifieri36329 [NCBI]
Taxonomic lineageiEukaryotaAlveolataApicomplexaAconoidasidaHaemosporidaPlasmodiidaePlasmodiumPlasmodium (Laverania)
Proteomesi
  • UP000001450 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiPlasmoDB:PF3D7_0408500.

Subcellular locationi

  • nucleolus UniRule annotation
  • nucleoplasm UniRule annotation
  • Mitochondrion UniRule annotation
  • Note: Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004035391 – 672Flap endonuclease 1Add BLAST672

Post-translational modificationi

Phosphorylated. Phosphorylation upon DNA damage induces relocalization to the nuclear plasma.UniRule annotation

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Interacts with PCNA. Three molecules of FEN1 bind to one PCNA trimer with each molecule binding to one PCNA monomer. PCNA stimulates the nuclease activity without altering cleavage specificity.UniRule annotation

Protein-protein interaction databases

BioGridi1207701. 1 interactor.
IntActiQ7K734. 1 interactor.
MINTiMINT-1729073.

Structurei

3D structure databases

ProteinModelPortaliQ7K734.
SMRiQ7K734.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 106N-domainAdd BLAST106
Regioni124 – 266I-domainAdd BLAST143
Regioni349 – 357Interaction with PCNAUniRule annotation9

Sequence similaritiesi

Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000193853.
InParanoidiQ7K734.
KOiK04799.
OMAiCIMAGSD.
PhylomeDBiQ7K734.

Family and domain databases

CDDicd09867. PIN_FEN1. 1 hit.
Gene3Di3.40.50.1010. 1 hit.
HAMAPiMF_00614. Fen. 1 hit.
InterProiView protein in InterPro
IPR036279. 5-3_exonuclease_C_sf.
IPR002421. 5-3_exonuclease_N.
IPR023426. Flap_endonuc.
IPR008918. HhH2.
IPR029060. PIN-like_dom_sf.
IPR006086. XPG-I_dom.
IPR006084. XPG/Rad2.
IPR019974. XPG_CS.
IPR006085. XPG_DNA_repair_N.
PANTHERiPTHR11081. PTHR11081. 3 hits.
PfamiView protein in Pfam
PF00867. XPG_I. 1 hit.
PF00752. XPG_N. 1 hit.
PRINTSiPR00853. XPGRADSUPER.
SMARTiView protein in SMART
SM00475. 53EXOc. 1 hit.
SM00279. HhH2. 1 hit.
SM00484. XPGI. 1 hit.
SM00485. XPGN. 1 hit.
SUPFAMiSSF47807. SSF47807. 1 hit.
SSF88723. SSF88723. 2 hits.
PROSITEiView protein in PROSITE
PS00841. XPG_1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7K734-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGIKGLTKFI ADAAPNAIKE IKIESLMGRI IAIDASMSLY QFIIAIRDSE
60 70 80 90 100
QYGNLTNESG ETTSHISGLM SRSIRLMENG LKPIYVFDGA PPELKGSELE
110 120 130 140 150
KRGEKRQKAE ELLKKAKEEG NLEEIKKQSG RTVRVTRKQN EEAKKLLTLM
160 170 180 190 200
GIPIIEAPCE AESQCAFLTK YNLAHATATE DADALVFGTK ILIRNLNANA
210 220 230 240 250
TSNQNKNKNN SKRGYILTEI NLEQVLKGLN LTMDEFIDFC ILCGCDYCDT
260 270 280 290 300
IKGIGSKTAY NLIKEYNCIE KIIENIDQNK YQVPSNFRFQ EARKSFINPN
310 320 330 340 350
VLPKEDIKID WNEPQIEELK HFLIKDYNFN ELRVTNYINR LLKARKVTTQ
360 370 380 390 400
RRLDNFFTAC TKKSTKLIVE ETKKEQTLPA RKGKKRPTAG DKNKQKAVKR
410 420 430 440 450
KIEQANANGH HKMKEENKSV DNEKNEDGIK SVDNEKNEDG IKSVDDEKNL
460 470 480 490 500
DDEKNLDDEK NKDDEKNKDD EKNKDDEKNK DDEKNKDDEK KSLDNFSSNL
510 520 530 540 550
FDSDKESESG NIIKNEKQNM DDEKINDNLP SLFGDHSRIR HTENKDNISD
560 570 580 590 600
INNNNNNNNS SSNNNNISNN HFNSVSSNST FNSSTKLKSE DTLKSNSPLK
610 620 630 640 650
EDSPNSYNNI KNNNHTININ SQINNHKEPI SNNNLNNINN STEIKKKNTL
660 670
FLLPFCPKDV TNVKKKKYTQ RC
Length:672
Mass (Da):76,681
Last modified:July 5, 2004 - v1
Checksum:i6C2CBDBB95F40042
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF093702 Genomic DNA. Translation: AAG37989.1.
AL844503 Genomic DNA. Translation: CAB62862.1.
RefSeqiXP_001351399.1. XM_001351363.1.

Genome annotation databases

EnsemblProtistsiCAB62862; CAB62862; PF3D7_0408500.
GeneDBiPF3D7_0408500.1:pep.
GeneIDi812356.
KEGGipfa:PFD0420c.

Similar proteinsi

Entry informationi

Entry nameiFEN1_PLAF7
AccessioniPrimary (citable) accession number: Q7K734
Secondary accession number(s): Q9U0K1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: July 5, 2004
Last modified: November 22, 2017
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families