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Protein

Sodium-dependent dopamine transporter

Gene

DAT

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sodium-dependent dopamine transporter which terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals (PubMed:11125028, PubMed:12606774, PubMed:24037379, PubMed:25970245). Also transports tyramine and norepinephrine, shows less efficient transport of octopamine and does not transport serotonin (PubMed:11125028, PubMed:12606774). Plays a role in the regulation of the rest/activity cycle (PubMed:16093388, PubMed:25232310).6 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi42Sodium 1; via carbonyl oxygenCombined sources2 Publications1
Metal bindingi44Sodium 2; via carbonyl oxygenCombined sources2 Publications1
Metal bindingi45Sodium 1; via carbonyl oxygenCombined sources2 Publications1
Binding sitei46SubstrateCombined sources1
Metal bindingi49Sodium 2Combined sources2 Publications1
Binding sitei117Substrate; via carbonyl oxygenCombined sources1
Binding sitei121SubstrateCombined sources1
Metal bindingi320Sodium 2Combined sources2 Publications1
Metal bindingi352Sodium 2Combined sources2 Publications1
Metal bindingi417Sodium 1; via carbonyl oxygenCombined sources2 Publications1
Metal bindingi420Sodium 1Combined sources2 Publications1
Metal bindingi421Sodium 1Combined sources2 Publications1

GO - Molecular functioni

  • cocaine binding Source: FlyBase
  • dopamine:sodium symporter activity Source: GO_Central
  • dopamine transmembrane transporter activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • adult locomotory behavior Source: FlyBase
  • circadian sleep/wake cycle Source: UniProtKB
  • dopamine transport Source: UniProtKB
  • dopamine uptake involved in synaptic transmission Source: GO_Central
  • regulation of presynaptic cytosolic calcium ion concentration Source: FlyBase
  • response to odorant Source: FlyBase
  • sleep Source: FlyBase
Complete GO annotation...

Keywords - Biological processi

Neurotransmitter transport, Symport, Transport

Keywords - Ligandi

Metal-binding, Sodium

Enzyme and pathway databases

ReactomeiR-DME-379401. Dopamine clearance from the synaptic cleft.
R-DME-442660. Na+/Cl- dependent neurotransmitter transporters.

Protein family/group databases

TCDBi2.A.22.1.7. the neurotransmitter:sodium symporter (nss) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium-dependent dopamine transporter1 Publication
Alternative name(s):
Protein fumin1 Publication
Gene namesi
Name:DATImported
Synonyms:fmn1 Publication
ORF Names:CG8380Imported
OrganismiDrosophila melanogaster (Fruit fly)Imported
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0034136. DAT.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 33Cytoplasmic3 PublicationsAdd BLAST33
Transmembranei34 – 59Helical; Name=13 PublicationsAdd BLAST26
Topological domaini60 – 63Extracellular3 Publications4
Transmembranei64 – 87Helical; Name=23 PublicationsAdd BLAST24
Topological domaini88 – 107Cytoplasmic3 PublicationsAdd BLAST20
Transmembranei108 – 138Helical; Name=33 PublicationsAdd BLAST31
Topological domaini139 – 235Extracellular3 PublicationsAdd BLAST97
Transmembranei236 – 256Helical; Name=43 PublicationsAdd BLAST21
Topological domaini257 – 259Cytoplasmic3 Publications3
Transmembranei260 – 284Helical; Name=53 PublicationsAdd BLAST25
Topological domaini285 – 308Extracellular3 PublicationsAdd BLAST24
Transmembranei309 – 334Helical; Name=62 PublicationsAdd BLAST26
Topological domaini335 – 340Cytoplasmic3 Publications6
Transmembranei341 – 364Helical; Name=72 PublicationsAdd BLAST24
Topological domaini365 – 404Extracellular3 PublicationsAdd BLAST40
Transmembranei405 – 430Helical; Name=82 PublicationsAdd BLAST26
Topological domaini431 – 445Cytoplasmic3 PublicationsAdd BLAST15
Transmembranei446 – 466Helical; Name=92 PublicationsAdd BLAST21
Topological domaini467Extracellular3 Publications1
Transmembranei468 – 494Helical; Name=102 PublicationsAdd BLAST27
Topological domaini495 – 524Cytoplasmic3 PublicationsAdd BLAST30
Transmembranei525 – 547Helical; Name=112 PublicationsAdd BLAST23
Topological domaini548 – 550Extracellular3 Publications3
Transmembranei551 – 571Helical; Name=122 PublicationsAdd BLAST21
Topological domaini572 – 631Cytoplasmic3 PublicationsAdd BLAST60

GO - Cellular componenti

  • integral component of plasma membrane Source: UniProtKB
  • neuron projection Source: GO_Central
  • presynapse Source: GOC
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

No effect on fertility or longevity but mutants display longer periods of daily activity than controls, reduced rest, enhanced sensitivity to mechanical stimuli when inactive and decreased rest rebound in response to rest deprivation (PubMed:16093388). Impaired aversive olfactory memory due to excessive dopaminergic signaling (PubMed:25232310). RNAi-mediated knockdown in neurons causes significant reduction in the sleep-like rest state with total daily resting periods decreased to about half of that of control flies (PubMed:25232310).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi72M → F: 3-fold increase in sensitivity to cocaine inhibition of transport. 1 Publication1
Mutagenesisi121D → G: Increased binding affinity for cocaine. 1 Publication1
Mutagenesisi426S → M: Increased binding affinity for cocaine. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004356261 – 631Sodium-dependent dopamine transporterAdd BLAST631

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi141N-linked (GlcNAc...); atypicalCombined sources1 Publication1
Disulfide bondi148 ↔ 157Combined sources4 Publications
Glycosylationi164N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi173N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi182N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi188N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi193N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi224N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi300N-linked (GlcNAc...)PROSITE-ProRule annotation1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9NB97.
PRIDEiQ7K4Y6.

Expressioni

Tissue specificityi

Expression is restricted to dopaminergic neurons in the central nervous system.1 Publication

Developmental stagei

Low levels in 0-4 hour embryos with strong expression in late embryos at 12-24 hours and during larval development. Expression decreases during pupal development and increases again in adult flies with heads showing higher levels than bodies.1 Publication

Gene expression databases

BgeeiFBgn0034136.
ExpressionAtlasiQ7K4Y6. baseline.

Interactioni

Protein-protein interaction databases

IntActiQ7K4Y6. 4 interactors.
STRINGi7227.FBpp0303037.

Structurei

Secondary structure

1631
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi33 – 44Combined sources12
Helixi48 – 51Combined sources4
Helixi53 – 59Combined sources7
Helixi62 – 65Combined sources4
Helixi66 – 75Combined sources10
Helixi77 – 91Combined sources15
Helixi95 – 102Combined sources8
Helixi104 – 107Combined sources4
Helixi108 – 120Combined sources13
Turni121 – 124Combined sources4
Helixi125 – 137Combined sources13
Beta strandi140 – 142Combined sources3
Helixi144 – 146Combined sources3
Beta strandi148 – 150Combined sources3
Beta strandi158 – 160Combined sources3
Beta strandi204 – 207Combined sources4
Beta strandi208 – 210Combined sources3
Helixi211 – 218Combined sources8
Turni219 – 221Combined sources3
Helixi223 – 225Combined sources3
Beta strandi227 – 230Combined sources4
Helixi237 – 254Combined sources18
Turni255 – 257Combined sources3
Helixi258 – 284Combined sources27
Helixi289 – 297Combined sources9
Helixi301 – 305Combined sources5
Helixi307 – 320Combined sources14
Beta strandi324 – 326Combined sources3
Helixi327 – 332Combined sources6
Helixi341 – 373Combined sources33
Helixi378 – 381Combined sources4
Helixi386 – 390Combined sources5
Helixi392 – 399Combined sources8
Beta strandi400 – 402Combined sources3
Helixi403 – 436Combined sources34
Helixi438 – 442Combined sources5
Helixi444 – 458Combined sources15
Helixi459 – 463Combined sources5
Helixi467 – 477Combined sources11
Beta strandi478 – 481Combined sources4
Helixi482 – 495Combined sources14
Turni496 – 499Combined sources4
Helixi500 – 511Combined sources12
Helixi517 – 524Combined sources8
Helixi526 – 540Combined sources15
Helixi554 – 568Combined sources15
Helixi570 – 579Combined sources10
Beta strandi580 – 582Combined sources3
Helixi586 – 593Combined sources8
Helixi597 – 600Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4M48X-ray2.96A21-601[»]
4XNUX-ray2.98A25-599[»]
4XNXX-ray3.00A25-601[»]
4XP1X-ray2.89A25-600[»]
4XP4X-ray2.80A21-601[»]
4XP5X-ray3.30A25-599[»]
4XP6X-ray3.10A25-599[»]
4XP9X-ray2.80C25-601[»]
4XPAX-ray2.95A21-601[»]
4XPBX-ray3.05A21-601[»]
4XPFX-ray3.27A21-601[»]
4XPGX-ray3.21A21-601[»]
4XPHX-ray2.90A25-599[»]
4XPTX-ray3.36A25-599[»]
ProteinModelPortaliQ7K4Y6.
SMRiQ7K4Y6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. [View classification]UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3659. Eukaryota.
COG0733. LUCA.
GeneTreeiENSGT00760000118857.
KOiK05036.
OrthoDBiEOG091G08PX.
PhylomeDBiQ7K4Y6.

Family and domain databases

InterProiIPR000175. Na/ntran_symport.
[Graphical view]
PANTHERiPTHR11616. PTHR11616. 2 hits.
PfamiPF00209. SNF. 1 hit.
[Graphical view]
PRINTSiPR00176. NANEUSMPORT.
PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7K4Y6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPTGHISKS KTPTPHDNDN NSISDERETW SGKVDFLLSV IGFAVDLANV
60 70 80 90 100
WRFPYLCYKN GGGAFLVPYG IMLVVGGIPL FYMELALGQH NRKGAITCWG
110 120 130 140 150
RLVPLFKGIG YAVVLIAFYV DFYYNVIIAW SLRFFFASFT NSLPWTSCNN
160 170 180 190 200
IWNTPNCRPF ESQNASRVPV IGNYSDLYAM GNQSLLYNET YMNGSSLDTS
210 220 230 240 250
AVGHVEGFQS AASEYFNRYI LELNRSEGIH DLGAIKWDMA LCLLIVYLIC
260 270 280 290 300
YFSLWKGIST SGKVVWFTAL FPYAVLLILL IRGLTLPGSF LGIQYYLTPN
310 320 330 340 350
FSAIYKAEVW VDAATQVFFS LGPGFGVLLA YASYNKYHNN VYKDALLTSF
360 370 380 390 400
INSATSFIAG FVIFSVLGYM AHTLGVRIED VATEGPGLVF VVYPAAIATM
410 420 430 440 450
PASTFWALIF FMMLLTLGLD SSFGGSEAII TALSDEFPKI KRNRELFVAG
460 470 480 490 500
LFSLYFVVGL ASCTQGGFYF FHLLDRYAAG YSILVAVFFE AIAVSWIYGT
510 520 530 540 550
NRFSEDIRDM IGFPPGRYWQ VCWRFVAPIF LLFITVYGLI GYEPLTYADY
560 570 580 590 600
VYPSWANALG WCIAGSSVVM IPAVAIFKLL STPGSLRQRF TILTTPWRDQ
610 620 630
QSMAMVLNGV TTEVTVVRLT DTETAKEPVD V
Length:631
Mass (Da):70,330
Last modified:October 3, 2006 - v1
Checksum:iE7582D17F81E31E6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16H → R in AAF76882 (PubMed:11125028).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF260833 mRNA. Translation: AAF76882.1.
AF439752 mRNA. Translation: AAL32055.1.
AE013599 Genomic DNA. Translation: AAF57986.2.
AY051579 mRNA. Translation: AAK93003.1.
RefSeqiNP_523763.2. NM_079039.4.
UniGeneiDm.2848.

Genome annotation databases

EnsemblMetazoaiFBtr0087125; FBpp0086271; FBgn0034136.
GeneIDi36849.
KEGGidme:Dmel_CG8380.
UCSCiCG8380-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF260833 mRNA. Translation: AAF76882.1.
AF439752 mRNA. Translation: AAL32055.1.
AE013599 Genomic DNA. Translation: AAF57986.2.
AY051579 mRNA. Translation: AAK93003.1.
RefSeqiNP_523763.2. NM_079039.4.
UniGeneiDm.2848.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4M48X-ray2.96A21-601[»]
4XNUX-ray2.98A25-599[»]
4XNXX-ray3.00A25-601[»]
4XP1X-ray2.89A25-600[»]
4XP4X-ray2.80A21-601[»]
4XP5X-ray3.30A25-599[»]
4XP6X-ray3.10A25-599[»]
4XP9X-ray2.80C25-601[»]
4XPAX-ray2.95A21-601[»]
4XPBX-ray3.05A21-601[»]
4XPFX-ray3.27A21-601[»]
4XPGX-ray3.21A21-601[»]
4XPHX-ray2.90A25-599[»]
4XPTX-ray3.36A25-599[»]
ProteinModelPortaliQ7K4Y6.
SMRiQ7K4Y6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ7K4Y6. 4 interactors.
STRINGi7227.FBpp0303037.

Protein family/group databases

TCDBi2.A.22.1.7. the neurotransmitter:sodium symporter (nss) family.

Proteomic databases

PaxDbiQ9NB97.
PRIDEiQ7K4Y6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0087125; FBpp0086271; FBgn0034136.
GeneIDi36849.
KEGGidme:Dmel_CG8380.
UCSCiCG8380-RA. d. melanogaster.

Organism-specific databases

CTDi36849.
FlyBaseiFBgn0034136. DAT.

Phylogenomic databases

eggNOGiKOG3659. Eukaryota.
COG0733. LUCA.
GeneTreeiENSGT00760000118857.
KOiK05036.
OrthoDBiEOG091G08PX.
PhylomeDBiQ7K4Y6.

Enzyme and pathway databases

ReactomeiR-DME-379401. Dopamine clearance from the synaptic cleft.
R-DME-442660. Na+/Cl- dependent neurotransmitter transporters.

Miscellaneous databases

GenomeRNAii36849.
PROiQ7K4Y6.

Gene expression databases

BgeeiFBgn0034136.
ExpressionAtlasiQ7K4Y6. baseline.

Family and domain databases

InterProiIPR000175. Na/ntran_symport.
[Graphical view]
PANTHERiPTHR11616. PTHR11616. 2 hits.
PfamiPF00209. SNF. 1 hit.
[Graphical view]
PRINTSiPR00176. NANEUSMPORT.
PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDAT_DROME
AccessioniPrimary (citable) accession number: Q7K4Y6
Secondary accession number(s): Q9NB97
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 17, 2016
Last sequence update: October 3, 2006
Last modified: November 30, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Miscellaneous

10-fold less sensitive to cocaine than mammalian dopamine transporter SLC6A3.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.