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Protein

Sodium-dependent dopamine transporter

Gene

DAT

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sodium-dependent dopamine transporter which terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals (PubMed:11125028, PubMed:12606774, PubMed:24037379, PubMed:25970245). Also transports tyramine and norepinephrine, shows less efficient transport of octopamine and does not transport serotonin (PubMed:11125028, PubMed:12606774). Plays a role in the regulation of the rest/activity cycle (PubMed:16093388, PubMed:25232310).6 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi42 – 421Sodium 1; via carbonyl oxygenCombined sources2 Publications
Metal bindingi44 – 441Sodium 2; via carbonyl oxygenCombined sources2 Publications
Metal bindingi45 – 451Sodium 1; via carbonyl oxygenCombined sources2 Publications
Binding sitei46 – 461SubstrateCombined sources
Metal bindingi49 – 491Sodium 2Combined sources2 Publications
Binding sitei117 – 1171Substrate; via carbonyl oxygenCombined sources
Binding sitei121 – 1211SubstrateCombined sources
Metal bindingi320 – 3201Sodium 2Combined sources2 Publications
Metal bindingi352 – 3521Sodium 2Combined sources2 Publications
Metal bindingi417 – 4171Sodium 1; via carbonyl oxygenCombined sources2 Publications
Metal bindingi420 – 4201Sodium 1Combined sources2 Publications
Metal bindingi421 – 4211Sodium 1Combined sources2 Publications

GO - Molecular functioni

  • cocaine binding Source: FlyBase
  • dopamine:sodium symporter activity Source: GO_Central
  • dopamine transmembrane transporter activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • circadian sleep/wake cycle Source: UniProtKB
  • dopamine transport Source: UniProtKB
  • dopamine uptake involved in synaptic transmission Source: GO_Central
  • regulation of presynaptic cytosolic calcium ion concentration Source: FlyBase
  • response to odorant Source: FlyBase
  • sleep Source: FlyBase
Complete GO annotation...

Keywords - Biological processi

Neurotransmitter transport, Symport, Transport

Keywords - Ligandi

Metal-binding, Sodium

Protein family/group databases

TCDBi2.A.22.1.7. the neurotransmitter:sodium symporter (nss) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium-dependent dopamine transporter1 Publication
Alternative name(s):
Protein fumin1 Publication
Gene namesi
Name:DATImported
Synonyms:fmn1 Publication
ORF Names:CG8380Imported
OrganismiDrosophila melanogaster (Fruit fly)Imported
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0034136. DAT.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3333Cytoplasmic3 PublicationsAdd
BLAST
Transmembranei34 – 5926Helical; Name=13 PublicationsAdd
BLAST
Topological domaini60 – 634Extracellular3 Publications
Transmembranei64 – 8724Helical; Name=23 PublicationsAdd
BLAST
Topological domaini88 – 10720Cytoplasmic3 PublicationsAdd
BLAST
Transmembranei108 – 13831Helical; Name=33 PublicationsAdd
BLAST
Topological domaini139 – 23597Extracellular3 PublicationsAdd
BLAST
Transmembranei236 – 25621Helical; Name=43 PublicationsAdd
BLAST
Topological domaini257 – 2593Cytoplasmic3 Publications
Transmembranei260 – 28425Helical; Name=53 PublicationsAdd
BLAST
Topological domaini285 – 30824Extracellular3 PublicationsAdd
BLAST
Transmembranei309 – 33426Helical; Name=62 PublicationsAdd
BLAST
Topological domaini335 – 3406Cytoplasmic3 Publications
Transmembranei341 – 36424Helical; Name=72 PublicationsAdd
BLAST
Topological domaini365 – 40440Extracellular3 PublicationsAdd
BLAST
Transmembranei405 – 43026Helical; Name=82 PublicationsAdd
BLAST
Topological domaini431 – 44515Cytoplasmic3 PublicationsAdd
BLAST
Transmembranei446 – 46621Helical; Name=92 PublicationsAdd
BLAST
Topological domaini467 – 4671Extracellular3 Publications
Transmembranei468 – 49427Helical; Name=102 PublicationsAdd
BLAST
Topological domaini495 – 52430Cytoplasmic3 PublicationsAdd
BLAST
Transmembranei525 – 54723Helical; Name=112 PublicationsAdd
BLAST
Topological domaini548 – 5503Extracellular3 Publications
Transmembranei551 – 57121Helical; Name=122 PublicationsAdd
BLAST
Topological domaini572 – 63160Cytoplasmic3 PublicationsAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: UniProtKB
  • neuron projection Source: GO_Central
  • presynapse Source: GOC
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

No effect on fertility or longevity but mutants display longer periods of daily activity than controls, reduced rest, enhanced sensitivity to mechanical stimuli when inactive and decreased rest rebound in response to rest deprivation (PubMed:16093388). Impaired aversive olfactory memory due to excessive dopaminergic signaling (PubMed:25232310). RNAi-mediated knockdown in neurons causes significant reduction in the sleep-like rest state with total daily resting periods decreased to about half of that of control flies (PubMed:25232310).2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi72 – 721M → F: 3-fold increase in sensitivity to cocaine inhibition of transport. 1 Publication
Mutagenesisi121 – 1211D → G: Increased binding affinity for cocaine. 1 Publication
Mutagenesisi426 – 4261S → M: Increased binding affinity for cocaine. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 631631Sodium-dependent dopamine transporterPRO_0000435626Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi141 – 1411N-linked (GlcNAc...)Combined sources1 Publication
Disulfide bondi148 ↔ 157Combined sources4 Publications
Glycosylationi164 – 1641N-linked (GlcNAc...)PROSITE-ProRule annotation
Glycosylationi173 – 1731N-linked (GlcNAc...)PROSITE-ProRule annotation
Glycosylationi182 – 1821N-linked (GlcNAc...)PROSITE-ProRule annotation
Glycosylationi188 – 1881N-linked (GlcNAc...)PROSITE-ProRule annotation
Glycosylationi193 – 1931N-linked (GlcNAc...)PROSITE-ProRule annotation
Glycosylationi224 – 2241N-linked (GlcNAc...)PROSITE-ProRule annotation
Glycosylationi300 – 3001N-linked (GlcNAc...)PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9NB97.
PRIDEiQ9NB97.

Expressioni

Tissue specificityi

Expression is restricted to dopaminergic neurons in the central nervous system.1 Publication

Developmental stagei

Low levels in 0-4 hour embryos with strong expression in late embryos at 12-24 hours and during larval development. Expression decreases during pupal development and increases again in adult flies with heads showing higher levels than bodies.1 Publication

Gene expression databases

BgeeiFBgn0034136.
ExpressionAtlasiQ7K4Y6. differential.

Interactioni

Protein-protein interaction databases

IntActiQ7K4Y6. 4 interactions.
STRINGi7227.FBpp0303037.

Structurei

Secondary structure

1
631
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi33 – 4412Combined sources
Helixi48 – 514Combined sources
Helixi53 – 597Combined sources
Helixi62 – 654Combined sources
Helixi66 – 7510Combined sources
Helixi77 – 9115Combined sources
Helixi95 – 1028Combined sources
Helixi104 – 1074Combined sources
Helixi108 – 12013Combined sources
Turni121 – 1244Combined sources
Helixi125 – 13713Combined sources
Beta strandi140 – 1423Combined sources
Helixi144 – 1463Combined sources
Beta strandi148 – 1503Combined sources
Beta strandi158 – 1603Combined sources
Beta strandi204 – 2074Combined sources
Beta strandi208 – 2103Combined sources
Helixi211 – 2188Combined sources
Turni219 – 2213Combined sources
Helixi223 – 2253Combined sources
Beta strandi227 – 2304Combined sources
Helixi237 – 25418Combined sources
Turni255 – 2573Combined sources
Helixi258 – 28427Combined sources
Helixi289 – 2979Combined sources
Helixi301 – 3055Combined sources
Helixi307 – 32014Combined sources
Beta strandi324 – 3263Combined sources
Helixi327 – 3326Combined sources
Helixi341 – 37333Combined sources
Helixi378 – 3814Combined sources
Helixi386 – 3905Combined sources
Helixi392 – 3998Combined sources
Beta strandi400 – 4023Combined sources
Helixi403 – 43634Combined sources
Helixi438 – 4425Combined sources
Helixi444 – 45815Combined sources
Helixi459 – 4635Combined sources
Helixi467 – 47711Combined sources
Beta strandi478 – 4814Combined sources
Helixi482 – 49514Combined sources
Turni496 – 4994Combined sources
Helixi500 – 51112Combined sources
Helixi517 – 5248Combined sources
Helixi526 – 54015Combined sources
Helixi554 – 56815Combined sources
Helixi570 – 57910Combined sources
Beta strandi580 – 5823Combined sources
Helixi586 – 5938Combined sources
Helixi597 – 6004Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4M48X-ray2.96A21-601[»]
4XNUX-ray2.98A25-599[»]
4XNXX-ray3.00A25-601[»]
4XP1X-ray2.89A25-600[»]
4XP4X-ray2.80A21-601[»]
4XP5X-ray3.30A25-599[»]
4XP6X-ray3.10A25-599[»]
4XP9X-ray2.80C25-601[»]
4XPAX-ray2.95A21-601[»]
4XPBX-ray3.05A21-601[»]
4XPFX-ray3.27A21-601[»]
4XPGX-ray3.21A21-601[»]
4XPHX-ray2.90A25-599[»]
4XPTX-ray3.36A25-599[»]
SMRiQ7K4Y6. Positions 25-601.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. [View classification]UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3659. Eukaryota.
COG0733. LUCA.
GeneTreeiENSGT00760000118857.
KOiK05036.
OrthoDBiEOG091G08PX.
PhylomeDBiQ7K4Y6.

Family and domain databases

InterProiIPR000175. Na/ntran_symport.
[Graphical view]
PANTHERiPTHR11616. PTHR11616. 2 hits.
PfamiPF00209. SNF. 1 hit.
[Graphical view]
PRINTSiPR00176. NANEUSMPORT.
PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7K4Y6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSPTGHISKS KTPTPHDNDN NSISDERETW SGKVDFLLSV IGFAVDLANV
60 70 80 90 100
WRFPYLCYKN GGGAFLVPYG IMLVVGGIPL FYMELALGQH NRKGAITCWG
110 120 130 140 150
RLVPLFKGIG YAVVLIAFYV DFYYNVIIAW SLRFFFASFT NSLPWTSCNN
160 170 180 190 200
IWNTPNCRPF ESQNASRVPV IGNYSDLYAM GNQSLLYNET YMNGSSLDTS
210 220 230 240 250
AVGHVEGFQS AASEYFNRYI LELNRSEGIH DLGAIKWDMA LCLLIVYLIC
260 270 280 290 300
YFSLWKGIST SGKVVWFTAL FPYAVLLILL IRGLTLPGSF LGIQYYLTPN
310 320 330 340 350
FSAIYKAEVW VDAATQVFFS LGPGFGVLLA YASYNKYHNN VYKDALLTSF
360 370 380 390 400
INSATSFIAG FVIFSVLGYM AHTLGVRIED VATEGPGLVF VVYPAAIATM
410 420 430 440 450
PASTFWALIF FMMLLTLGLD SSFGGSEAII TALSDEFPKI KRNRELFVAG
460 470 480 490 500
LFSLYFVVGL ASCTQGGFYF FHLLDRYAAG YSILVAVFFE AIAVSWIYGT
510 520 530 540 550
NRFSEDIRDM IGFPPGRYWQ VCWRFVAPIF LLFITVYGLI GYEPLTYADY
560 570 580 590 600
VYPSWANALG WCIAGSSVVM IPAVAIFKLL STPGSLRQRF TILTTPWRDQ
610 620 630
QSMAMVLNGV TTEVTVVRLT DTETAKEPVD V
Length:631
Mass (Da):70,330
Last modified:October 3, 2006 - v1
Checksum:iE7582D17F81E31E6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti16 – 161H → R in AAF76882 (PubMed:11125028).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF260833 mRNA. Translation: AAF76882.1.
AF439752 mRNA. Translation: AAL32055.1.
AE013599 Genomic DNA. Translation: AAF57986.2.
AY051579 mRNA. Translation: AAK93003.1.
RefSeqiNP_523763.2. NM_079039.4.
UniGeneiDm.2848.

Genome annotation databases

EnsemblMetazoaiFBtr0087125; FBpp0086271; FBgn0034136.
GeneIDi36849.
KEGGidme:Dmel_CG8380.
UCSCiCG8380-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF260833 mRNA. Translation: AAF76882.1.
AF439752 mRNA. Translation: AAL32055.1.
AE013599 Genomic DNA. Translation: AAF57986.2.
AY051579 mRNA. Translation: AAK93003.1.
RefSeqiNP_523763.2. NM_079039.4.
UniGeneiDm.2848.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4M48X-ray2.96A21-601[»]
4XNUX-ray2.98A25-599[»]
4XNXX-ray3.00A25-601[»]
4XP1X-ray2.89A25-600[»]
4XP4X-ray2.80A21-601[»]
4XP5X-ray3.30A25-599[»]
4XP6X-ray3.10A25-599[»]
4XP9X-ray2.80C25-601[»]
4XPAX-ray2.95A21-601[»]
4XPBX-ray3.05A21-601[»]
4XPFX-ray3.27A21-601[»]
4XPGX-ray3.21A21-601[»]
4XPHX-ray2.90A25-599[»]
4XPTX-ray3.36A25-599[»]
SMRiQ7K4Y6. Positions 25-601.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ7K4Y6. 4 interactions.
STRINGi7227.FBpp0303037.

Protein family/group databases

TCDBi2.A.22.1.7. the neurotransmitter:sodium symporter (nss) family.

Proteomic databases

PaxDbiQ9NB97.
PRIDEiQ9NB97.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0087125; FBpp0086271; FBgn0034136.
GeneIDi36849.
KEGGidme:Dmel_CG8380.
UCSCiCG8380-RA. d. melanogaster.

Organism-specific databases

CTDi36849.
FlyBaseiFBgn0034136. DAT.

Phylogenomic databases

eggNOGiKOG3659. Eukaryota.
COG0733. LUCA.
GeneTreeiENSGT00760000118857.
KOiK05036.
OrthoDBiEOG091G08PX.
PhylomeDBiQ7K4Y6.

Miscellaneous databases

GenomeRNAii36849.
PROiQ7K4Y6.

Gene expression databases

BgeeiFBgn0034136.
ExpressionAtlasiQ7K4Y6. differential.

Family and domain databases

InterProiIPR000175. Na/ntran_symport.
[Graphical view]
PANTHERiPTHR11616. PTHR11616. 2 hits.
PfamiPF00209. SNF. 1 hit.
[Graphical view]
PRINTSiPR00176. NANEUSMPORT.
PROSITEiPS00610. NA_NEUROTRAN_SYMP_1. 1 hit.
PS00754. NA_NEUROTRAN_SYMP_2. 1 hit.
PS50267. NA_NEUROTRAN_SYMP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDAT_DROME
AccessioniPrimary (citable) accession number: Q7K4Y6
Secondary accession number(s): Q9NB97
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 17, 2016
Last sequence update: October 3, 2006
Last modified: September 7, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Miscellaneous

10-fold less sensitive to cocaine than mammalian dopamine transporter SLC6A3.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.