Reviewed, UniProtKB/Swiss-Prot Q7K2D2 (DCTN2_DROME)

Names and origin

Protein names

Recommended name:
    Probable dynactin subunit 2
      Short name=Dynamitin

Gene names

Name:	Dmn
ORF Names:	CG8269

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	380 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. May play a role in synapse formation during brain development By similarity.
Subunit structure	Subunit of dynactin, a multiprotein complex associated with dynein By similarity.
Subcellular location	Cytoplasm › cytoskeleton By similarity. Membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the dynactin subunit 2 family.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Dynein Membrane Microtubule
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB centrosome localization Inferred from mutant phenotype. Source: FlyBase mitosis Inferred from sequence or structural similarity. Source: UniProtKB mitotic spindle organization Inferred from mutant phenotype. Source: FlyBase
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell dynactin complex Inferred from sequence or structural similarity. Source: UniProtKB dynein complex Inferred from electronic annotation. Source: UniProtKB-KW membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microtubule Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	motor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 380	380	Probable dynactin subunit 2		PRO_0000288772
Regions
	Coiled coil	100 – 135	36	Potential
	Coiled coil	353 – 377	25	Potential
Amino acid modifications
	Modified residue	49	1	Phosphoserine Ref.4
	Modified residue	58	1	Phosphoserine Ref.4
	Modified residue	86	1	Phosphoserine Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q7K2D2-1 [UniParc]. Last modified October 3, 2006. Version 1. Checksum: CF7E1D3BFF5989C5	FASTA	380	41,998
	10 20 30 40 50 60 MADPKFQNLP GIAYDQPDVY ETPDDPELDT SDYYEEEPEN EAIERLHISP SVAHKRFSGA 70 80 90 100 110 120 TVEGSVDFTD RIGRRMCRGY DTRGSSDYEL VGQGEKETPV QKCQRLQIEM NELLNEVAAL 130 140 150 160 170 180 QVDRKVADEE KQSYDAVATV ISTARKVLES LKLEQVLGKE QTPGSKQVKA LISQVEEFKQ 190 200 210 220 230 240 SGVLTAIPTP GTDLAATARV ASLEQRISQL EKVLGAQPDK LSRLTAATNT TNVLEAVRHL 250 260 270 280 290 300 STKAALIQPD KLDTIEQRLT SLAGKMDAIA EKSSGSAQDA KRDQKITELY DIAKRTEPVV 310 320 330 340 350 360 EILPHVIERM QALEALHKYA NNFAKIIAEI EQKQGTITTS LVNNKELLHS VQETFAQNLE 370 380 TINSKVAKVE QRVAAISSAK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[3]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[4]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-49; SER-58 AND SER-86, MASS SPECTROMETRY. Tissue: Embryo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE013599 Genomic DNA. Translation: AAF59034.1. AY061092 mRNA. Translation: AAL28640.1.
RefSeq	NP_524690.1.
UniGene	Dm.1423
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q7K2D2.
Genome annotation databases
Ensembl	FBgn0021825. Drosophila melanogaster. [Contig view]
GeneID	44086.
KEGG	dme:Dmel_CG8269.
NMPDR	fig|7227.3.peg.3944.
Organism-specific databases
FlyBase	FBgn0021825. Dmn.
Phylogenomic databases
OMA	Q7K2D2. THLDTTQ.
Family and domain databases
InterPro	IPR006996. Dynamitin. [Graphical view]
Pfam	PF04912. Dynamitin. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	836720.

Entry information

Entry name

DCTN2_DROME

Accession

Primary (citable) accession number: Q7K2D2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	October 3, 2006
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project