Q7JQ07 (MOS1T_DROMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mariner Mos1 transposase
EC=3.1.-.-

Alternative name(s):
Transposable element Mos1 transposase

Gene names

Name:

mariner\T

Organism

Drosophila mauritiana (Fruit fly)

Taxonomic identifier

7226 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mediates transposition of transposon Mos1 by a 'cut and paste' mechanism. Transposases are sequence-specific nucleases and strand transferases that catalyze transposition through an ordered series of events: sequence-specific binding of transposase to the terminal inverted repeats (IR) present at each end of the transposon, pairing of the transposon IRs in a paired-end complex (PEC), cleavage of one or both DNA strands at each transposon end, capture of target DNA, and strand transfer to insert the transposon at a new site. Ref.3
Cofactor	Binds 2 divalent metal cations per subunit. Magnesium or manganese.
Subunit structure	Homodimer. The complex has a trans arrangement, with each transposon end recognized by the DNA binding region of one transposase monomer and by the active site of the other monomer. Ref.3
Subcellular location	Nucleus Probable.

Ontologies

Keywords
Biological process	DNA integration DNA recombination
Cellular component	Nucleus
Ligand	DNA-binding Magnesium Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	3D-structure Transposable element
Gene Ontology (GO)
Biological process	DNA integration Inferred from electronic annotation. Source: UniProtKB-KW DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 345

345

Mariner Mos1 transposase

PRO_0000386644

Regions

DNA binding

24 – 55

32

H-T-H motif

DNA binding

89 – 110

22

H-T-H motif

Region

1 – 112

112

DNA-binding

Region

113 – 125

13

Linker

Region

126 – 345

220

Catalytic

Sites

Metal binding

156

1

Magnesium 1

Metal binding

156

1

Magnesium 2

Metal binding

249

1

Magnesium 1

Metal binding

284

1

Magnesium 2

Site

48

1

Important for base-specific DNA-binding

Site

100

1

Important for base-specific DNA-binding

Site

118

1

Important for base-specific DNA-binding

Site

186

1

Critical for target DNA recognition

Site

186

1

Important for target DNA recognition and for strand transfer activity

Site

293

1

Important for base-specific DNA-binding

Experimental info

Mutagenesis

48

1

R → Q: Loss of DNA binding; when associated with R-100.

Mutagenesis

100

1

Q → R: Loss of DNA binding; when associated with Q-48.

Mutagenesis

118

1

R → A: Reduces rate of second strand cleavage; when associated with A-216.

Mutagenesis

119

1

W → P: Alters cleavage sites in second strand cleavage.

Mutagenesis

186

1

R → A: No effect on second strand cleavage. Strongly reduced strand transfer activity.

Mutagenesis

216

1

T → A: Reduces rate of second strand cleavage; when associated with A-118.

Mutagenesis

284

1

D → A: Loss of catalytic activity.

Sequence conflict

152

1

I → L in AAA28701. Ref.2

Sequence conflict

164

1

S → N in AAA28698. Ref.2

Sequence conflict

164

1

S → N in AAA28699. Ref.2

Sequence conflict

164

1

S → N in AAA28700. Ref.2

Sequence conflict

164

1

S → N in AAA28701. Ref.2

Sequence conflict

164

1

S → N in AAA28702. Ref.2

Sequence conflict

164

1

S → N in AAA28703. Ref.2

Sequence conflict

210

1

R → P in AAA28698. Ref.2

Sequence conflict

210

1

R → P in AAA28699. Ref.2

Sequence conflict

210

1

R → P in AAA28700. Ref.2

Sequence conflict

210

1

R → P in AAA28701. Ref.2

Sequence conflict

210

1

R → P in AAA28702. Ref.2

Sequence conflict

210

1

R → P in AAA28703. Ref.2

Sequence conflict

243

1

R → K in AAA28703. Ref.2

Sequence conflict

248 – 249

2

HD → YG in AAA28703. Ref.2

Sequence conflict

260

1

R → C in AAA28703. Ref.2

Secondary structure

1

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345

Helix Strand Turn

Details...

Helix

8 – 20

13

Helix

25 – 35

11

Helix

43 – 53

11

Helix

74 – 83

10

Helix

89 – 95

7

Helix

100 – 109

10

Beta strand

113 – 115

3

Beta strand

119 – 122

4

Helix

126 – 145

20

Helix

149 – 151

3

Beta strand

152 – 154

3

Beta strand

156 – 162

7

Beta strand

170 – 172

3

Beta strand

190 – 198

9

Beta strand

204 – 208

5

Helix

216 – 233

18

Turn

252 – 255

4

Helix

257 – 265

9

Helix

278 – 280

3

Helix

282 – 285

4

Helix

287 – 290

4

Turn

294 – 297

4

Helix

303 – 316

14

Helix

320 – 323

4

Helix

329 – 338

10

Sequences

	Sequence		Length	Mass (Da)
	Q7JQ07 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: BC8E59E33B966697	FASTA	345	40,851
	10 20 30 40 50 60 MSSFVPNKEQ TRTVLIFCFH LKKTAAESHR MLVEAFGEQV PTVKKCERWF QRFKSGDFDV 70 80 90 100 110 120 DDKEHGKPPK RYEDAELQAL LDEDDAQTQK QLAEQLEVSQ QAVSNRLREM GKIQKVGRWV 130 140 150 160 170 180 PHELNERQME RRKNTCEILL SRYKRKSFLH RIVTGDEKWI FFVSPKRKKS YVDPGQPATS 190 200 210 220 230 240 TARPNRFGKK TMLCVWWDQS GVIYYELLKR GETVNTARYQ QQLINLNRAL QRKRPEYQKR 250 260 270 280 290 300 QHRVIFLHDN APSHTARAVR DTLETLNWEV LPHAAYSPDL APSDYHLFAS MGHALAEQRF 310 320 330 340 DSYESVKKWL DEWFAAKDDE FYWRGIHKLP ERWEKCVASD GKYLE

References

[1]	"Molecular structure of a somatically unstable transposable element in Drosophila." Jacobson J.W., Medhora M.M., Hartl D.L. Proc. Natl. Acad. Sci. U.S.A. 83:8684-8688(1986) [PubMed: 3022302] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The mariner transposable element is widespread in insects." Robertson H.M. Nature 362:241-245(1993) [PubMed: 8384700] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 125-275.
[3]	"Molecular architecture of the Mos1 paired-end complex: the structural basis of DNA transposition in a eukaryote." Richardson J.M., Colloms S.D., Finnegan D.J., Walkinshaw M.D. Cell 138:1096-1108(2009) [PubMed: 19766564] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.25 ANGSTROMS) OF MUTANT ALA-216 IN COMPLEX WITH INVERTED REPEAT DNA; MAGNESIUM AND MANGANESE IONS, FUNCTION, SUBUNIT, METAL-BINDING SITES.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M14653 Genomic DNA. Translation: AAA28678.1.
L10449 Genomic DNA. Translation: AAA28698.1.
L10450 Genomic DNA. Translation: AAA28699.1.
L10451 Genomic DNA. Translation: AAA28700.1.
L10452 Genomic DNA. Translation: AAA28701.1.
L10453 Genomic DNA. Translation: AAA28702.1.
L10454 Genomic DNA. Translation: AAA28703.1.

PIR

S36997.
S37002.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3HOS	X-ray	3.50	A/B	1-343	[»]
3HOT	X-ray	3.25	A/B	1-343	[»]

ProteinModelPortal

Q7JQ07.

SMR

Q7JQ07. Positions 119-345.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Organism-specific databases

FlyBase

FBgn0013835. Dmau\mariner\T.

Family and domain databases

InterPro

IPR001888. Transposase_1.
IPR011991. WHTH_trsnscrt_rep_DNA-bd.
[Graphical view]

Gene3D

G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.

Pfam

PF01359. Transposase_1. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

MOS1T_DROMA

Accession

Primary (citable) accession number: Q7JQ07
Secondary accession number(s): Q05407, Q05415, Q05417

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	July 5, 2004
Last modified:	November 30, 2010
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project