Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q7JJ13 (BRD2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bromodomain-containing protein 2
Alternative name(s):
Female sterile homeotic-related protein 1
Fsrg-1
Protein RING3
Gene names
Name:Brd2
Synonyms:Fsrg1, Kiaa4005, Ring3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length798 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling. Regulates transcription of the CCND1 gene. Plays a role in nucleosome assembly By similarity. May play a role in spermatogenesis or folliculogenesis. Ref.1 Ref.2

Subunit structure

Homodimer. Interacts with E2F1 and with histone H4 acetylated at 'Lys-13' By similarity.

Subcellular location

Nucleus. Note: Detected on chromatin and nucleosomes By similarity. Ref.1 Ref.2

Tissue specificity

Predominantly expressed in the testis, followed by ovary, placenta, embryo and to a lower extent in somatic tissues. Ref.1 Ref.2

Domain

One bromodomain is sufficient for a partial interaction with histone H4 acetylated at 'Lys-13' By similarity.

Sequence similarities

Contains 2 bromo domains.

Contains 1 NET domain.

Sequence caution

The sequence BAD90273.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q7JJ13-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q7JJ13-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-46: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 798798Bromodomain-containing protein 2
PRO_0000274005

Regions

Domain90 – 16273Bromo 1
Domain363 – 43573Bromo 2
Domain630 – 71283NET
Motif553 – 5575Nuclear localization signal Potential
Compositional bias60 – 634Poly-Pro
Compositional bias228 – 331104Pro-rich
Compositional bias478 – 52346Glu-rich
Compositional bias549 – 5579Poly-Lys
Compositional bias589 – 5924Poly-Gly
Compositional bias632 – 6365Poly-Glu
Compositional bias761 – 79737Ser-rich

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue61Phosphothreonine By similarity
Modified residue361Phosphoserine By similarity
Modified residue2971Phosphoserine Ref.8
Modified residue3001Phosphoserine Ref.8
Modified residue3041Phosphoserine By similarity
Modified residue6311Phosphoserine By similarity

Natural variations

Alternative sequence1 – 4646Missing in isoform 2.
VSP_022601

Experimental info

Sequence conflict1691E → K in BAE28228. Ref.5
Sequence conflict1991A → G in AAC24810. Ref.1
Sequence conflict6911P → L in BAD90273. Ref.6
Sequence conflict765 – 7673QVA → LVP in AAC24810. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 08DD57FBF1385E96

FASTA79888,067
        10         20         30         40         50         60 
MLQNVTPHKL PGEGNAGLLG LGPEAAAPGK RIRKPSLLYE GFESPTMASV PALQLAPANP 

        70         80         90        100        110        120 
PPPEVSNPKK PGRVTNQLQY LHKVVMKALW KHQFAWPFRQ PVDAVKLGLP DYHKIIKQPM 

       130        140        150        160        170        180 
DMGTIKRRLE NNYYWAASEC MQDFNTMFTN CYIYNKPTDD IVLMAQTLEK IFLQKVASMP 

       190        200        210        220        230        240 
QEEQELVVTI PKNSHKKGAK LAALQGSITS AHQVPAVSSV SHTALYTPPP EIPTTVLNIP 

       250        260        270        280        290        300 
HPSVISSPLL KSLHSAGPPL LAVSAAPPAQ PLAKKKGVKR KADTTTPTPT AILAPGSPAS 

       310        320        330        340        350        360 
PPGSLEPKAA RLPPMRRESG RPIKPPRKDL PDSQQQHQSS KKGKLSEQLK HCNGILKELL 

       370        380        390        400        410        420 
SKKHAAYAWP FYKPVDASAL GLHDYHDIIK HPMDLSTVKR KMENRDYRDA QEFAADVRLM 

       430        440        450        460        470        480 
FSNCYKYNPP DHDVVAMARK LQDVFEFRYA KMPDEPLEPG PLPVSTALPP GLTKSSSESS 

       490        500        510        520        530        540 
SEESSSESSS EEEEEEEEDE DEEESESSDS EEERAHRLAE LQEQLRAVHE QLAALSQGPI 

       550        560        570        580        590        600 
SKPKRKREKK EKKKKRKAEK HRGRIGIDED DKGPRAPRPP QPKKSKKAGG GGSNATTLSH 

       610        620        630        640        650        660 
PGFGTSGGSS NKLPKKSQKT APPVLPTGYD SEEEEESRPM SYDEKRQLSL DINKLPGEKL 

       670        680        690        700        710        720 
GRVVHIIQAR EPSLRDSNPE EIEIDFETLK PSTLRELERY VLSCLRKKPR KPYTIRKPVG 

       730        740        750        760        770        780 
KTKEELALEK KRELEKRLQD VSGQLNSTKK PPKKASEKTE SSAQQVAVSR LSASSSSSDS 

       790 
SSSSSSSSSS DTSDSDSG 

« Hide

Isoform 2 [UniParc].

Checksum: DA931FC6F03FA1FD
Show »

FASTA75283,271

References

« Hide 'large scale' references
[1]"Expression and potential role of Fsrg1, a murine bromodomain-containing homologue of the Drosophila gene female sterile homeotic."
Rhee K., Brunori M., Besset V., Trousdale R., Wolgemuth D.J.
J. Cell Sci. 111:3541-3550(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION, FUNCTION.
Tissue: Testis.
[2]"Nucleotide sequence of the ring3 gene in the class II region of the mouse MHC and its abundant expression in testicular germ cells."
Taniguchi Y., Matsuzaka Y., Fujimoto H., Miyado K., Kohda A., Okumura K., Kimura M., Inoko H.
Genomics 51:114-123(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 1-549 (ISOFORM 2), TISSUE SPECIFICITY, SUBCELLULAR LOCATION, FUNCTION.
Strain: 129/SvJ and CD-1.
Tissue: Testis.
[3]"DNA sequence and structure of the mouse RING3 gene: identification of variant RING3 transcripts."
Thorpe K.L., Beck S.
Immunogenetics 48:82-86(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Sequence of the mouse major histocompatibility locus class II region."
Rowen L., Qin S., Madan A., Loretz C., James R., Dors M., Mix L., Hall J., Lasky S., Hood L.
Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 129/SvJ.
[5]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Amnion and Visual cortex.
[6]"Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Fetal brain.
[7]"Complete sequence of UL26B06."
Korf I.
Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-503.
Strain: C57BL/6.
[8]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-297 AND SER-300, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF045462 mRNA. Translation: AAC24810.1.
AB010246 mRNA. Translation: BAA24377.1.
AB010247 mRNA. Translation: BAA24378.1.
AB010248 mRNA. Translation: BAA24379.1.
AB212273 mRNA. Translation: BAD97682.1.
D89801 Genomic DNA. Translation: BAA25416.1.
AL009226 Genomic DNA. Translation: CAA15818.1.
AL009226 Genomic DNA. Translation: CAA15819.1.
AF100956 Genomic DNA. Translation: AAC69907.1.
AK147918 mRNA. Translation: BAE28228.1.
AK158970 mRNA. Translation: BAE34749.1.
AK168525 mRNA. Translation: BAE40404.1.
AK220444 mRNA. Translation: BAD90273.1. Different initiation.
AF318183 mRNA. Translation: AAK07919.1.
RefSeqNP_001191902.1. NM_001204973.1.
NP_034368.2. NM_010238.3.
XP_006523721.1. XM_006523658.1.
XP_006525365.1. XM_006525302.1.
UniGeneMm.3444.

3D structure databases

ProteinModelPortalQ7JJ13.
SMRQ7JJ13. Positions 71-188, 345-454, 635-712.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid199750. 1 interaction.
IntActQ7JJ13. 1 interaction.
MINTMINT-4104990.

PTM databases

PhosphoSiteQ7JJ13.

Proteomic databases

PaxDbQ7JJ13.
PRIDEQ7JJ13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000025193; ENSMUSP00000025193; ENSMUSG00000024335. [Q7JJ13-1]
ENSMUST00000114242; ENSMUSP00000109880; ENSMUSG00000024335. [Q7JJ13-1]
GeneID14312.
KEGGmmu:14312.
UCSCuc008cbi.1. mouse. [Q7JJ13-1]

Organism-specific databases

CTD6046.
MGIMGI:99495. Brd2.
RougeSearch...

Phylogenomic databases

eggNOGCOG5076.
GeneTreeENSGT00730000110626.
HOVERGENHBG004896.
InParanoidQ7JJ13.
KOK08871.
OMAHREEDSE.
OrthoDBEOG7TTQ86.
PhylomeDBQ7JJ13.
TreeFamTF317345.

Gene expression databases

ArrayExpressQ7JJ13.
BgeeQ7JJ13.
CleanExMM_BRD2.
GenevestigatorQ7JJ13.

Family and domain databases

Gene3D1.20.920.10. 2 hits.
InterProIPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
IPR027353. NET_dom.
[Graphical view]
PfamPF00439. Bromodomain. 2 hits.
[Graphical view]
PRINTSPR00503. BROMODOMAIN.
SMARTSM00297. BROMO. 2 hits.
[Graphical view]
SUPFAMSSF47370. SSF47370. 2 hits.
PROSITEPS00633. BROMODOMAIN_1. 2 hits.
PS50014. BROMODOMAIN_2. 2 hits.
PS51525. NET. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSBRD2. mouse.
NextBio285739.
PROQ7JJ13.
SOURCESearch...

Entry information

Entry nameBRD2_MOUSE
AccessionPrimary (citable) accession number: Q7JJ13
Secondary accession number(s): O54795 expand/collapse secondary AC list , O88411, Q3UGI0, Q5DTS6, Q794H7, Q794H9, Q99PC5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot