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Protein

Bromodomain-containing protein 2

Gene

Brd2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling. Regulates transcription of the CCND1 gene. Plays a role in nucleosome assembly (By similarity). May play a role in spermatogenesis or folliculogenesis.By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Bromodomain-containing protein 2
Alternative name(s):
Female sterile homeotic-related protein 1
Fsrg-1
Protein RING3
Gene namesi
Name:Brd2
Synonyms:Fsrg1, Kiaa4005, Ring3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:99495. Brd2.

Subcellular locationi

  • Nucleus 2 Publications

  • Note: Detected on chromatin and nucleosomes.By similarity

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 798798Bromodomain-containing protein 2PRO_0000274005Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei6 – 61PhosphothreonineBy similarity
Modified residuei36 – 361PhosphoserineBy similarity
Modified residuei297 – 2971PhosphoserineCombined sources
Modified residuei300 – 3001PhosphoserineCombined sources
Modified residuei304 – 3041PhosphoserineCombined sources
Modified residuei631 – 6311PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ7JJ13.
MaxQBiQ7JJ13.
PaxDbiQ7JJ13.
PRIDEiQ7JJ13.

PTM databases

iPTMnetiQ7JJ13.
PhosphoSiteiQ7JJ13.

Expressioni

Tissue specificityi

Predominantly expressed in the testis, followed by ovary, placenta, embryo and to a lower extent in somatic tissues.2 Publications

Gene expression databases

BgeeiQ7JJ13.
CleanExiMM_BRD2.
ExpressionAtlasiQ7JJ13. baseline and differential.
GenevisibleiQ7JJ13. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with E2F1 and with histone H4 acetylated at 'Lys-13' (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199750. 1 interaction.
IntActiQ7JJ13. 1 interaction.
MINTiMINT-4104990.
STRINGi10090.ENSMUSP00000025193.

Structurei

3D structure databases

ProteinModelPortaliQ7JJ13.
SMRiQ7JJ13. Positions 71-188, 343-454, 635-712.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini90 – 16273Bromo 1PROSITE-ProRule annotationAdd
BLAST
Domaini363 – 43573Bromo 2PROSITE-ProRule annotationAdd
BLAST
Domaini630 – 71283NETPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi553 – 5575Nuclear localization signalSequence analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi60 – 634Poly-Pro
Compositional biasi228 – 331104Pro-richAdd
BLAST
Compositional biasi478 – 52346Glu-richAdd
BLAST
Compositional biasi549 – 5579Poly-Lys
Compositional biasi589 – 5924Poly-Gly
Compositional biasi632 – 6365Poly-Glu
Compositional biasi761 – 79737Ser-richAdd
BLAST

Domaini

One bromodomain is sufficient for a partial interaction with histone H4 acetylated at 'Lys-13'.By similarity

Sequence similaritiesi

Contains 2 bromo domains.PROSITE-ProRule annotation
Contains 1 NET domain.PROSITE-ProRule annotation

Keywords - Domaini

Bromodomain, Repeat

Phylogenomic databases

eggNOGiKOG1474. Eukaryota.
COG5076. LUCA.
GeneTreeiENSGT00760000119206.
HOVERGENiHBG004896.
InParanoidiQ7JJ13.
KOiK08871.
OMAiTESSVAQ.
OrthoDBiEOG7TTQ86.
PhylomeDBiQ7JJ13.
TreeFamiTF317345.

Family and domain databases

Gene3Di1.20.920.10. 2 hits.
InterProiIPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
IPR027353. NET_dom.
[Graphical view]
PfamiPF17035. BET. 1 hit.
PF00439. Bromodomain. 2 hits.
[Graphical view]
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00297. BROMO. 2 hits.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 2 hits.
PROSITEiPS00633. BROMODOMAIN_1. 2 hits.
PS50014. BROMODOMAIN_2. 2 hits.
PS51525. NET. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q7JJ13-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLQNVTPHKL PGEGNAGLLG LGPEAAAPGK RIRKPSLLYE GFESPTMASV
60 70 80 90 100
PALQLAPANP PPPEVSNPKK PGRVTNQLQY LHKVVMKALW KHQFAWPFRQ
110 120 130 140 150
PVDAVKLGLP DYHKIIKQPM DMGTIKRRLE NNYYWAASEC MQDFNTMFTN
160 170 180 190 200
CYIYNKPTDD IVLMAQTLEK IFLQKVASMP QEEQELVVTI PKNSHKKGAK
210 220 230 240 250
LAALQGSITS AHQVPAVSSV SHTALYTPPP EIPTTVLNIP HPSVISSPLL
260 270 280 290 300
KSLHSAGPPL LAVSAAPPAQ PLAKKKGVKR KADTTTPTPT AILAPGSPAS
310 320 330 340 350
PPGSLEPKAA RLPPMRRESG RPIKPPRKDL PDSQQQHQSS KKGKLSEQLK
360 370 380 390 400
HCNGILKELL SKKHAAYAWP FYKPVDASAL GLHDYHDIIK HPMDLSTVKR
410 420 430 440 450
KMENRDYRDA QEFAADVRLM FSNCYKYNPP DHDVVAMARK LQDVFEFRYA
460 470 480 490 500
KMPDEPLEPG PLPVSTALPP GLTKSSSESS SEESSSESSS EEEEEEEEDE
510 520 530 540 550
DEEESESSDS EEERAHRLAE LQEQLRAVHE QLAALSQGPI SKPKRKREKK
560 570 580 590 600
EKKKKRKAEK HRGRIGIDED DKGPRAPRPP QPKKSKKAGG GGSNATTLSH
610 620 630 640 650
PGFGTSGGSS NKLPKKSQKT APPVLPTGYD SEEEEESRPM SYDEKRQLSL
660 670 680 690 700
DINKLPGEKL GRVVHIIQAR EPSLRDSNPE EIEIDFETLK PSTLRELERY
710 720 730 740 750
VLSCLRKKPR KPYTIRKPVG KTKEELALEK KRELEKRLQD VSGQLNSTKK
760 770 780 790
PPKKASEKTE SSAQQVAVSR LSASSSSSDS SSSSSSSSSS DTSDSDSG
Length:798
Mass (Da):88,067
Last modified:July 5, 2004 - v1
Checksum:i08DD57FBF1385E96
GO
Isoform 2 (identifier: Q7JJ13-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-46: Missing.

Note: No experimental confirmation available.
Show »
Length:752
Mass (Da):83,271
Checksum:iDA931FC6F03FA1FD
GO

Sequence cautioni

The sequence BAD90273.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti169 – 1691E → K in BAE28228 (PubMed:16141072).Curated
Sequence conflicti199 – 1991A → G in AAC24810 (PubMed:9811568).Curated
Sequence conflicti691 – 6911P → L in BAD90273 (Ref. 6) Curated
Sequence conflicti765 – 7673QVA → LVP in AAC24810 (PubMed:9811568).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 4646Missing in isoform 2. 1 PublicationVSP_022601Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF045462 mRNA. Translation: AAC24810.1.
AB010246 mRNA. Translation: BAA24377.1.
AB010247 mRNA. Translation: BAA24378.1.
AB010248 mRNA. Translation: BAA24379.1.
AB212273 mRNA. Translation: BAD97682.1.
D89801 Genomic DNA. Translation: BAA25416.1.
AL009226 Genomic DNA. Translation: CAA15818.1.
AL009226 Genomic DNA. Translation: CAA15819.1.
AF100956 Genomic DNA. Translation: AAC69907.1.
AK147918 mRNA. Translation: BAE28228.1.
AK158970 mRNA. Translation: BAE34749.1.
AK168525 mRNA. Translation: BAE40404.1.
AK220444 mRNA. Translation: BAD90273.1. Different initiation.
AF318183 mRNA. Translation: AAK07919.1.
CCDSiCCDS28641.1. [Q7JJ13-1]
RefSeqiNP_001191902.1. NM_001204973.1. [Q7JJ13-1]
NP_034368.2. NM_010238.3. [Q7JJ13-1]
XP_006523721.1. XM_006523658.2. [Q7JJ13-2]
XP_006525365.1. XM_006525302.2. [Q7JJ13-2]
UniGeneiMm.3444.

Genome annotation databases

EnsembliENSMUST00000025193; ENSMUSP00000025193; ENSMUSG00000024335. [Q7JJ13-1]
ENSMUST00000114242; ENSMUSP00000109880; ENSMUSG00000024335. [Q7JJ13-1]
GeneIDi14312.
KEGGimmu:14312.
UCSCiuc008cbi.1. mouse. [Q7JJ13-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF045462 mRNA. Translation: AAC24810.1.
AB010246 mRNA. Translation: BAA24377.1.
AB010247 mRNA. Translation: BAA24378.1.
AB010248 mRNA. Translation: BAA24379.1.
AB212273 mRNA. Translation: BAD97682.1.
D89801 Genomic DNA. Translation: BAA25416.1.
AL009226 Genomic DNA. Translation: CAA15818.1.
AL009226 Genomic DNA. Translation: CAA15819.1.
AF100956 Genomic DNA. Translation: AAC69907.1.
AK147918 mRNA. Translation: BAE28228.1.
AK158970 mRNA. Translation: BAE34749.1.
AK168525 mRNA. Translation: BAE40404.1.
AK220444 mRNA. Translation: BAD90273.1. Different initiation.
AF318183 mRNA. Translation: AAK07919.1.
CCDSiCCDS28641.1. [Q7JJ13-1]
RefSeqiNP_001191902.1. NM_001204973.1. [Q7JJ13-1]
NP_034368.2. NM_010238.3. [Q7JJ13-1]
XP_006523721.1. XM_006523658.2. [Q7JJ13-2]
XP_006525365.1. XM_006525302.2. [Q7JJ13-2]
UniGeneiMm.3444.

3D structure databases

ProteinModelPortaliQ7JJ13.
SMRiQ7JJ13. Positions 71-188, 343-454, 635-712.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199750. 1 interaction.
IntActiQ7JJ13. 1 interaction.
MINTiMINT-4104990.
STRINGi10090.ENSMUSP00000025193.

PTM databases

iPTMnetiQ7JJ13.
PhosphoSiteiQ7JJ13.

Proteomic databases

EPDiQ7JJ13.
MaxQBiQ7JJ13.
PaxDbiQ7JJ13.
PRIDEiQ7JJ13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025193; ENSMUSP00000025193; ENSMUSG00000024335. [Q7JJ13-1]
ENSMUST00000114242; ENSMUSP00000109880; ENSMUSG00000024335. [Q7JJ13-1]
GeneIDi14312.
KEGGimmu:14312.
UCSCiuc008cbi.1. mouse. [Q7JJ13-1]

Organism-specific databases

CTDi6046.
MGIiMGI:99495. Brd2.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1474. Eukaryota.
COG5076. LUCA.
GeneTreeiENSGT00760000119206.
HOVERGENiHBG004896.
InParanoidiQ7JJ13.
KOiK08871.
OMAiTESSVAQ.
OrthoDBiEOG7TTQ86.
PhylomeDBiQ7JJ13.
TreeFamiTF317345.

Miscellaneous databases

ChiTaRSiBrd2. mouse.
PROiQ7JJ13.
SOURCEiSearch...

Gene expression databases

BgeeiQ7JJ13.
CleanExiMM_BRD2.
ExpressionAtlasiQ7JJ13. baseline and differential.
GenevisibleiQ7JJ13. MM.

Family and domain databases

Gene3Di1.20.920.10. 2 hits.
InterProiIPR001487. Bromodomain.
IPR018359. Bromodomain_CS.
IPR027353. NET_dom.
[Graphical view]
PfamiPF17035. BET. 1 hit.
PF00439. Bromodomain. 2 hits.
[Graphical view]
PRINTSiPR00503. BROMODOMAIN.
SMARTiSM00297. BROMO. 2 hits.
[Graphical view]
SUPFAMiSSF47370. SSF47370. 2 hits.
PROSITEiPS00633. BROMODOMAIN_1. 2 hits.
PS50014. BROMODOMAIN_2. 2 hits.
PS51525. NET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression and potential role of Fsrg1, a murine bromodomain-containing homologue of the Drosophila gene female sterile homeotic."
    Rhee K., Brunori M., Besset V., Trousdale R., Wolgemuth D.J.
    J. Cell Sci. 111:3541-3550(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, SUBCELLULAR LOCATION, FUNCTION.
    Tissue: Testis.
  2. "Nucleotide sequence of the ring3 gene in the class II region of the mouse MHC and its abundant expression in testicular germ cells."
    Taniguchi Y., Matsuzaka Y., Fujimoto H., Miyado K., Kohda A., Okumura K., Kimura M., Inoko H.
    Genomics 51:114-123(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 1-549 (ISOFORM 2), TISSUE SPECIFICITY, SUBCELLULAR LOCATION, FUNCTION.
    Strain: 129/SvJ and CD-1.
    Tissue: Testis.
  3. "DNA sequence and structure of the mouse RING3 gene: identification of variant RING3 transcripts."
    Thorpe K.L., Beck S.
    Immunogenetics 48:82-86(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Sequence of the mouse major histocompatibility locus class II region."
    Rowen L., Qin S., Madan A., Loretz C., James R., Dors M., Mix L., Hall J., Lasky S., Hood L.
    Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 129/SvJ.
  5. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Amnion and Visual cortex.
  6. "Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
    Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Fetal brain.
  7. "Complete sequence of UL26B06."
    Korf I.
    Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-503.
    Strain: C57BL/6J.
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-297 AND SER-300, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  9. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-297; SER-300 AND SER-304, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Kidney, Liver, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiBRD2_MOUSE
AccessioniPrimary (citable) accession number: Q7JJ13
Secondary accession number(s): O54795
, O88411, Q3UGI0, Q5DTS6, Q794H7, Q794H9, Q99PC5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2007
Last sequence update: July 5, 2004
Last modified: June 8, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.