Q7GB25 (AB5C_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ABC transporter C family member 5
Short name=ABC transporter ABCC.5
Short name=AtABCC5
EC=3.6.3.44

Alternative name(s):
ATP-energized glutathione S-conjugate pump 5
Glutathione S-conjugate-transporting ATPase 5
Multidrug resistance-associated protein 5

Gene names

Name:	ABCC5
Synonyms:	MRP5
Ordered Locus Names:	At1g04120
ORF Names:	F20D22.11

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	1514 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Pump for glutathione S-conjugates. Involved in regulation of K+ and Na+ cell content. Mediates resistance to NaCl and Li+, confers sensitivity to sulfonylurea drugs such as glibenclamide (inducer of stomatal opening), and required for stomatal opening regulation by auxin, abscisic acid (ABA) and external Ca2+. Transports oestradiol-17-(beta-D-glucuronide) (E217G). Involved in the root auxin content regulation that controls the transition from primary root elongation to lateral root formation. Plays a role in ABA-mediated germination inhibition. Ref.6 Ref.8 Ref.10 Ref.11
Catalytic activity	ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).
Enzyme regulation	(E217G) transport activity in negatively regulated by organic anions such as oestradiol-3-sulfate, luteolin-7-O-diglucuronide-4'-O-glucuronide, glycocholate, vanadate and the sulfonylurea glibenclamide, and, to a lower extent, by bafilomycin A1, NH4Cl, GSH, GSSG and DNB-GS. Ref.6
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Tissue specificity	Ubiquitous, mostly in vascular tissues and epidermis, including guard cells. Ref.6 Ref.9 Ref.11
Developmental stage	In flowers, present in anthers central vascular strand of the filament and in connecting tissues of the pollen sacs. Also present at the silique attachment site of the pedicel. Ref.6
Disruption phenotype	Increase water use efficiency. Ref.10
Sequence similarities	Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily. [View classification] Contains 2 ABC transmembrane type-1 domains. Contains 2 ABC transporter domains.
Sequence caution	The sequence AAC16754.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Coiled coil Repeat Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular potassium ion homeostasis Inferred from mutant phenotype Ref.11. Source: TAIR response to salt stress Inferred from mutant phenotype Ref.11. Source: TAIR
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plant-type vacuole Inferred from direct assay. Source: TAIR vacuolar membrane Inferred from direct assay. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW sulfonylurea receptor activity Inferred from direct assay Ref.11. Source: TAIR xenobiotic-transporting ATPase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q7GB25-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1514	1514	ABC transporter C family member 5		PRO_0000226076
Regions
	Transmembrane	16 – 36	21	Helical; Potential
	Transmembrane	76 – 96	21	Helical; Potential
	Transmembrane	111 – 131	21	Helical; Potential
	Transmembrane	142 – 162	21	Helical; Potential
	Transmembrane	177 – 197	21	Helical; Potential
	Transmembrane	312 – 332	21	Helical; Potential
	Transmembrane	334 – 354	21	Helical; Potential
	Transmembrane	421 – 441	21	Helical; Potential
	Transmembrane	446 – 466	21	Helical; Potential
	Transmembrane	533 – 553	21	Helical; Potential
	Transmembrane	946 – 966	21	Helical; Potential
	Transmembrane	986 – 1006	21	Helical; Potential
	Transmembrane	1078 – 1098	21	Helical; Potential
	Transmembrane	1117 – 1137	21	Helical; Potential
	Transmembrane	1155 – 1175	21	Helical; Potential
	Transmembrane	1180 – 1200	21	Helical; Potential
	Domain	307 – 588	282	ABC transmembrane type-1 1
	Domain	622 – 845	224	ABC transporter 1
	Domain	949 – 1231	283	ABC transmembrane type-1 2
	Domain	1268 – 1502	235	ABC transporter 2
	Nucleotide binding	657 – 664	8	ATP 1 Potential
	Nucleotide binding	1302 – 1309	8	ATP 2 Potential
	Coiled coil	899 – 927	29	Potential

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 7, 2006. Version 2. Checksum: 35351F361D101783	FASTA	1,514	168,574
	10 20 30 40 50 60 MDFIEISLIF REHLPLLELC SVIINLLLFL VFLFAVSARQ ILVCVRRGRD RLSKDDTVSA 70 80 90 100 110 120 SNLSLEREVN HVSVGFGFNL SLLCCLYVLG VQVLVLVYDG VKVRREVSDW FVLCFPASQS 130 140 150 160 170 180 LAWFVLSFLV LHLKYKSSEK LPFLVRIWWF LAFSICLCTM YVDGRRLAIE GWSRCSSHVV 190 200 210 220 230 240 ANLAVTPALG FLCFLAWRGV SGIQVTRSSS DLQEPLLVEE EAACLKVTPY STAGLVSLIT 250 260 270 280 290 300 LSWLDPLLSA GSKRPLELKD IPLLAPRDRA KSSYKVLKSN WKRCKSENPS KPPSLARAIM 310 320 330 340 350 360 KSFWKEAACN AVFAGLNTLV SYVGPYLISY FVDYLGGKEI FPHEGYVLAG IFFTSKLIET 370 380 390 400 410 420 VTTRQWYMGV DILGMHVRSA LTAMVYRKGL KLSSIAKQNH TSGEIVNYMA VDVQRIGDYS 430 440 450 460 470 480 WYLHDIWMLP MQIVLALAIL YKSVGIAAVA TLVATIISIL VTIPLAKVQE DYQDKLMTAK 490 500 510 520 530 540 DERMRKTSEC LRNMRVLKLQ AWEDRYRVRL EEMREEEYGW LRKALYSQAF VTFIFWSSPI 550 560 570 580 590 600 FVAAVTFATS IFLGTQLTAG GVLSALATFR ILQEPLRNFP DLVSMMAQTK VSLDRISGFL 610 620 630 640 650 660 QEEELQEDAT VVIPRGLSNI AIEIKDGVFC WDPFSSRPTL SGIQMKVEKG MRVAVCGTVG 670 680 690 700 710 720 SGKSSFISCI LGEIPKISGE VRICGTTGYV SQSAWIQSGN IEENILFGSP MEKTKYKNVI 730 740 750 760 770 780 QACSLKKDIE LFSHGDQTII GERGINLSGG QKQRVQLARA LYQDADIYLL DDPFSALDAH 790 800 810 820 830 840 TGSDLFRDYI LSALAEKTVV FVTHQVEFLP AADLILVLKE GRIIQSGKYD DLLQAGTDFK 850 860 870 880 890 900 ALVSAHHEAI EAMDIPSPSS EDSDENPIRD SLVLHNPKSD VFENDIETLA KEVQEGGSAS 910 920 930 940 950 960 DLKAIKEKKK KAKRSRKKQL VQEEERVKGK VSMKVYLSYM GAAYKGALIP LIILAQAAFQ 970 980 990 1000 1010 1020 FLQIASNWWM AWANPQTEGD ESKVDPTLLL IVYTALAFGS SVFIFVRAAL VATFGLAAAQ 1030 1040 1050 1060 1070 1080 KLFLNMLRSV FRAPMSFFDS TPAGRILNRV SIDQSVVDLD IPFRLGGFAS TTIQLCGIVA 1090 1100 1110 1120 1130 1140 VMTNVTWQVF LLVVPVAVAC FWMQKYYMAS SRELVRIVSI QKSPIIHLFG ESIAGAATIR 1150 1160 1170 1180 1190 1200 GFGQEKRFIK RNLYLLDCFV RPFFCSIAAI EWLCLRMELL STLVFAFCMV LLVSFPHGTI 1210 1220 1230 1240 1250 1260 DPSMAGLAVT YGLNLNGRLS RWILSFCKLE NKIISIERIY QYSQIVGEAP AIIEDFRPPS 1270 1280 1290 1300 1310 1320 SWPATGTIEL VDVKVRYAEN LPTVLHGVSC VFPGGKKIGI VGRTGSGKST LIQALFRLIE 1330 1340 1350 1360 1370 1380 PTAGKITIDN IDISQIGLHD LRSRLGIIPQ DPTLFEGTIR ANLDPLEEHS DDKIWEALDK 1390 1400 1410 1420 1430 1440 SQLGDVVRGK DLKLDSPVLE NGDNWSVGQR QLVSLGRALL KQAKILVLDE ATASVDTATD 1450 1460 1470 1480 1490 1500 NLIQKIIRTE FEDCTVCTIA HRIPTVIDSD LVLVLSDGRV AEFDTPARLL EDKSSMFLKL 1510 VTEYSSRSTG IPEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Weigmann N., Ansorge M., Meuller-Roeber B. Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. Tissue: Hypocotyl.
[2]	"Cloning and characterization of putative sulfonylurea recepter-like gene from Arabidopsis thaliana." Ko J.-H., Kwon M.J., Yi H.C., Cho M.H. Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Wassilewskija.
[3]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1438-1514. Strain: cv. Columbia.
[6]	"The Arabidopsis thaliana ABC transporter AtMRP5 controls root development and stomata movement." Gaedeke N., Klein M., Kolukisaoglu U.H., Forestier C., Mueller A., Ansorge M., Becker D., Mamnun Y., Kuchler K., Schulz B., Mueller-Roeber B., Martinoia E. EMBO J. 20:1875-1887(2001) [PubMed: 11296221] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, ENZYME REGULATION.
[7]	"The Arabidopsis thaliana ABC protein superfamily, a complete inventory." Sanchez-Fernandez R., Davies T.G., Coleman J.O., Rea P.A. J. Biol. Chem. 276:30231-30244(2001) [PubMed: 11346655] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[8]	"Multifunctionality of plant ABC transporters -- more than just detoxifiers." Martinoia E., Klein M., Geisler M., Bovet L., Forestier C., Kolukisaoglu H.U., Mueller-Roeber B., Schulz B. Planta 214:345-355(2002) [PubMed: 11855639] [Abstract] Cited for: FUNCTION, GENE FAMILY.
[9]	"Family business: the multidrug-resistance related protein (MRP) ABC transporter genes in Arabidopsis thaliana." Kolukisaoglu U.H., Bovet L., Klein M., Eggmann T., Geisler M., Wanke D., Martinoia E., Schulz B. Planta 216:107-119(2002) [PubMed: 12430019] [Abstract] Cited for: TISSUE SPECIFICITY.
[10]	"The plant multidrug resistance ABC transporter AtMRP5 is involved in guard cell hormonal signalling and water use." Klein M., Perfus-Barbeoch L., Frelet A., Gaedeke N., Reinhardt D., Mueller-Roeber B., Martinoia E., Forestier C. Plant J. 33:119-129(2003) [PubMed: 12943546] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[11]	"Binding of sulfonylurea by AtMRP5, an Arabidopsis multidrug resistance-related protein that functions in salt tolerance." Lee E.K., Kwon M.J., Ko J.-H., Yi H.C., Hwang M.G., Chang S., Cho M.H. Plant Physiol. 134:528-538(2004) [PubMed: 14684837] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[12]	"Plant ABC proteins - a unified nomenclature and updated inventory." Verrier P.J., Bird D., Burla B., Dassa E., Forestier C., Geisler M., Klein M., Kolukisaoglu H.U., Lee Y., Martinoia E., Murphy A., Rea P.A., Samuels L., Schulz B., Spalding E.J., Yazaki K., Theodoulou F.L. Trends Plant Sci. 13:151-159(2008) [PubMed: 18299247] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y11250 mRNA. Translation: CAA72120.1. AF225908 mRNA. Translation: AAG14965.1. AC002411 Genomic DNA. Translation: AAC16754.1. Sequence problems. CP002684 Genomic DNA. Translation: AEE27658.1. AK220840 mRNA. Translation: BAD94167.1.
IPI	IPI00543326.
PIR	T00961. T52080.
RefSeq	NP_171908.1. NM_100293.2.
UniGene	At.5395.
3D structure databases
ProteinModelPortal	Q7GB25.
SMR	Q7GB25. Positions 296-847, 932-1504.
ModBase	Search...
Protein family/group databases
TCDB	3.A.1.208.22. ATP-binding cassette (ABC) superfamily.
Proteomic databases
PRIDE	Q7GB25.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G04120.1; AT1G04120.1; AT1G04120.
GeneID	839277.
GenomeReviews	Gene locus AT1G04120 in contig CT485782_GR.
KEGG	ath:AT1G04120.
NMPDR	fig|3702.1.peg.557.
Organism-specific databases
TAIR	At1g04120.
Phylogenomic databases
eggNOG	KOG0054.
GeneTree	EPGT00070000028299.
HOGENOM	HBG758042.
InParanoid	Q7GB25.
OMA	VFVTHQV.
PhylomeDB	Q7GB25.
ProtClustDB	CLSN2681764.
Gene expression databases
Genevestigator	Q7GB25.
GermOnline	AT1G04120. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR017940. ABC_transporter_type1. IPR001140. ABC_transptr_TM_dom. IPR011527. ABC_transptrTM_dom_typ1. IPR003593. ATPase_AAA+_core. [Graphical view]
Pfam	PF00664. ABC_membrane. 2 hits. PF00005. ABC_tran. 2 hits. [Graphical view]
SMART	SM00382. AAA. 2 hits. [Graphical view]
SUPFAM	SSF90123. ABC_TM_1. 2 hits.
PROSITE	PS50929. ABC_TM1F. 2 hits. PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AB5C_ARATH

Accession

Primary (citable) accession number: Q7GB25
Secondary accession number(s): O65619, Q56ZX0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	March 7, 2006
Last modified:	November 16, 2011
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program