Reviewed, UniProtKB/Swiss-Prot Q7G765 (NADO2_ORYSJ)

Names and origin

Protein names

Recommended name:
    Probable NAD(P)H-dependent oxidoreductase 2
    EC=1.-.-.-

Gene names

Ordered Locus Names:	Os10g0113100, LOC_Os10g02390
ORF Names:	OJ1014H12.12, OSJNBb0012A20.19

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	322 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May play a role in auxin-induced cell growth by generating hydroxyl radicals, which tends to increase cell wall loosening By similarity.
Sequence similarities	Belongs to the aldo/keto reductase family.

Ontologies

Keywords
Ligand	NAD NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 322	322	Probable NAD(P)H-dependent oxidoreductase 2		PRO_0000124656
Regions
	Nucleotide binding	216 – 275	60	NADP By similarity
Sites
	Active site	57	1	Proton donor By similarity
	Binding site	120	1	Substrate By similarity
	Site	87	1	Lowers pKa of active site Tyr By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7G765-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 0B2EA244B66742C9	FASTA	322	35,784
	10 20 30 40 50 60 MAMATIPEVP ASALLPTMPR IGMGTAAFPF TSSEETTAAL LRAIELGYRH FDTARLYATE 70 80 90 100 110 120 GCVSEAVAEA VRRGLVASRA DVFVTSKLWC SDLHAGRVVP AARETLRNLG MDYVDLLLVH 130 140 150 160 170 180 WPATVAPGSY DFPFPKEEMA PAFDMEGVWR GMEECHRLGL ARAIGVSNFS AKKLEQLLSF 190 200 210 220 230 240 AVVRPAANQV EMNPMWQQRT LREVCRREGV QLCGYSPLGA KGTPWGSAAV MDSGVLHDIA 250 260 270 280 290 300 QTKGKTLAQI CLRWMYEQGD VLLVKTYNEN RMKENLDIFD WELTEEERDK ISKLPQQRGL 310 320 TGMQFVCDNG PYKCVEDLWD GA

References

[1]

"In-depth view of structure, activity, and evolution of rice chromosome 10." Yu Y., Rambo T., Currie J., Saski C., Kim H.-R., Collura K., Thompson S., Simmons J., Yang T.-J., Nah G., Patel A.J., Thurmond S., Henry D., Oates R., Palmer M., Pries G., Gibson J., Anderson H. , Paradkar M., Crane L., Dale J., Carver M.B., Wood T., Frisch D., Engler F., Soderlund C., Palmer L.E., Teytelman L., Nascimento L., De la Bastide M., Spiegel L., Ware D., O'Shaughnessy A., Dike S., Dedhia N., Preston R., Huang E., Ferraro K., Kuit K., Miller B., Zutavern T., Katzenberger F., Muller S., Balija V., Martienssen R.A., Stein L., Minx P., Johnson D., Cordum H., Mardis E., Cheng Z., Jiang J., Wilson R., McCombie W.R., Wing R.A., Yuan Q., Ouyang S., Liu J., Jones K.M., Gansberger K., Moffat K., Hill J., Tsitrin T., Overton L., Bera J., Kim M., Jin S., Tallon L., Ciecko A., Pai G., Van Aken S., Utterback T., Reidmuller S., Bormann J., Feldblyum T., Hsiao J., Zismann V., Blunt S., de Vazeille A.R., Shaffer T., Koo H., Suh B., Yang Q., Haas B., Peterson J., Pertea M., Volfovsky N., Wortman J., White O., Salzberg S.L., Fraser C.M., Buell C.R., Messing J., Song R., Fuks G., Llaca V., Kovchak S., Young S., Bowers J.E., Paterson A.H., Johns M.A., Mao L., Pan H., Dean R.A. Science 300:1566-1569(2003) [PubMed: 12791992] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

[2]

"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC079685 Genomic DNA. Translation: AAK52588.1. AC098694 Genomic DNA. Translation: AAM44873.1. AP008216 Genomic DNA. Translation: BAF25958.1. DP000086 Genomic DNA. Translation: AAP51851.1. AK103772 mRNA. No translation available.
RefSeq	NP_001064044.1.
UniGene	Os.46268
3D structure databases
HSSP	HSSP built from PDB template 1HW6 based on UniProtKB P06632.
ModBase	Search...
Genome annotation databases
GeneID	4347978.
KEGG	osa:4347978.
NMPDR	fig|39947.1.peg.11111.
Organism-specific databases
Gramene	Q7G765.
Family and domain databases
InterPro	IPR001395. Aldo/ket_red. IPR018170. Aldo/ket_reductase_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.100. Aldo/ket_red. 1 hit.
PANTHER	PTHR11732. Aldo/ket_red. 1 hit.
Pfam	PF00248. Aldo_ket_red. 1 hit. [Graphical view]
ProDom	PD000288. Aldo/ket_red. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00798. ALDOKETO_REDUCTASE_1. 1 hit. PS00062. ALDOKETO_REDUCTASE_2. 1 hit. PS00063. ALDOKETO_REDUCTASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NADO2_ORYSJ

Accession

Primary (citable) accession number: Q7G765
Secondary accession number(s): Q0IZA7, Q94LH8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	July 5, 2004
Last modified:	June 16, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)