Reviewed, UniProtKB/Swiss-Prot Q7G764 (NADO1_ORYSJ)

Names and origin

Protein names

Recommended name:
    Probable NAD(P)H-dependent oxidoreductase 1
    EC=1.1.1.-

Gene names

Ordered Locus Names:	Os10g0113000, LOC_Os10g02380
ORF Names:	OJ1014H12.13, OSJNBb0012A20.18

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May play a role in auxin-induced cell growth by generating hydroxyl radicals, which tends to increase cell wall loosening. Ref.3
Tissue specificity	Expressed in roots and leaf sheaths. Ref.3
Induction	By auxin and gibberellic acid. Ref.3
Sequence similarities	Belongs to the aldo/keto reductase family.

Ontologies

Keywords
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 321	321	Probable NAD(P)H-dependent oxidoreductase 1		PRO_0000124655
Regions
	Nucleotide binding	214 – 273	60	NADP By similarity
Sites
	Active site	55	1	Proton donor By similarity
	Binding site	118	1	Substrate By similarity
	Site	85	1	Lowers pKa of active site Tyr By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7G764-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 2B7906FED1FFCC34	FASTA	321	35,713
	10 20 30 40 50 60 MATIPEVPAS ELIQTMPRVG MGTAAFPFTS SEDTTAAMLR AIELGYRHFD TARIYATEGC 70 80 90 100 110 120 VGEAVAEAVR RGLIASRADV FVTSKIWCSD LHAGRVVPAA RETLRNLGMD YVDLLLVHWP 130 140 150 160 170 180 VSLTPGNYDF PFPKEVILPS FDMEGVWRGM EECHRLGLAR AIGVSNFSAK KLEQLLSLAA 190 200 210 220 230 240 VRPAVNQVEV NPMWQQRTLR EVCRREGVQL CGYSPLGAKG TPWGSAAVMD SGVLQEIAGA 250 260 270 280 290 300 KGKTLAQICL RWLYEQGDVL LVKTYNEKRM KENLDIFNWE LTDEERERIS QLPQLRGLPG 310 320 LEFISDHGPY KSVEDLWDGD V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"In-depth view of structure, activity, and evolution of rice chromosome 10." Yu Y., Rambo T., Currie J., Saski C., Kim H.-R., Collura K., Thompson S., Simmons J., Yang T.-J., Nah G., Patel A.J., Thurmond S., Henry D., Oates R., Palmer M., Pries G., Gibson J., Anderson H. , Paradkar M., Crane L., Dale J., Carver M.B., Wood T., Frisch D., Engler F., Soderlund C., Palmer L.E., Teytelman L., Nascimento L., De la Bastide M., Spiegel L., Ware D., O'Shaughnessy A., Dike S., Dedhia N., Preston R., Huang E., Ferraro K., Kuit K., Miller B., Zutavern T., Katzenberger F., Muller S., Balija V., Martienssen R.A., Stein L., Minx P., Johnson D., Cordum H., Mardis E., Cheng Z., Jiang J., Wilson R., McCombie W.R., Wing R.A., Yuan Q., Ouyang S., Liu J., Jones K.M., Gansberger K., Moffat K., Hill J., Tsitrin T., Overton L., Bera J., Kim M., Jin S., Tallon L., Ciecko A., Pai G., Van Aken S., Utterback T., Reidmuller S., Bormann J., Feldblyum T., Hsiao J., Zismann V., Blunt S., de Vazeille A.R., Shaffer T., Koo H., Suh B., Yang Q., Haas B., Peterson J., Pertea M., Volfovsky N., Wortman J., White O., Salzberg S.L., Fraser C.M., Buell C.R., Messing J., Song R., Fuks G., Llaca V., Kovchak S., Young S., Bowers J.E., Paterson A.H., Johns M.A., Mao L., Pan H., Dean R.A. Science 300:1566-1569(2003) [PubMed: 12791992] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[3]	"Characterization of NADPH-dependent oxidoreductase induced by auxin in rice." Oguchi K., Tanaka N., Komatsu S., Akao S. Physiol. Plantarum 121:124-131(2004) [PubMed: 15086826] [Abstract] Cited for: PROTEIN SEQUENCE OF 12-21; 68-76; 153-162 AND 237-256, FUNCTION, INDUCTION, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC079685 Genomic DNA. Translation: AAK52587.1. AC098694 Genomic DNA. Translation: AAM44874.1. AP008216 Genomic DNA. Translation: BAF25957.1. DP000086 Genomic DNA. Translation: AAP51850.1. AK061106 mRNA. No translation available. AK102609 mRNA. No translation available.
RefSeq	NP_001064043.1.
UniGene	Os.4020
3D structure databases
HSSP	HSSP built from PDB template 1HW6 based on UniProtKB P06632.
ModBase	Search...
Genome annotation databases
GeneID	4347977.
KEGG	osa:4347977.
NMPDR	fig|39947.1.peg.11110.
Organism-specific databases
Gramene	Q7G764.
Family and domain databases
InterPro	IPR001395. Aldo/ket_red. IPR018170. Aldo/ket_reductase_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.100. Aldo/ket_red. 1 hit.
PANTHER	PTHR11732. Aldo/ket_red. 1 hit.
Pfam	PF00248. Aldo_ket_red. 1 hit. [Graphical view]
ProDom	PD000288. Aldo/ket_red. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00798. ALDOKETO_REDUCTASE_1. 1 hit. PS00062. ALDOKETO_REDUCTASE_2. 1 hit. PS00063. ALDOKETO_REDUCTASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NADO1_ORYSJ

Accession

Primary (citable) accession number: Q7G764
Secondary accession number(s): Q0IZA8, Q94LH9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	July 5, 2004
Last modified:	June 16, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)