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Protein

Benzaldehyde dehydrogenase (NAD(+))

Gene

AAO4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the accumulation of benzoic acid (BA) in siliques.1 Publication

Catalytic activityi

Benzaldehyde + NAD+ + H2O = benzoate + NADH.1 Publication
(Indol-3-yl)acetaldehyde + H2O + O2 = (indol-3-yl)acetate + H2O2.1 Publication

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterBy similarityNote: Binds 2 [2Fe-2S] clusters.By similarity
  • FADBy similarity
  • Mo-molybdopterinBy similarityNote: Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.By similarity

Enzyme regulationi

Inhibited by Cu2+.1 Publication

Kineticsi

Kcat is 95.2 sec(-1) with benzoic acid (BA) as substrate, 253.9 sec(-1) with NAD+ as substrate, 1.904 sec(-1) with indole-3-acetaldehyde as substrate, and 436.5 sec(-1) with cinnamylaldehyde as substrate (at pH 7, PubMed:19297586).
  1. KM=23 µM for benzoic acid (BA) (at pH7, in the presence of NAD+)1 Publication
  2. KM=58.1 µM for NAD+ (at pH7, in the presence of benzoic acid (BA))1 Publication
  3. KM=2.07 µM for indole-3-acetaldehyde (at pH7)1 Publication
  4. KM=103.9 µM for cinnamylaldehyde (at pH7)1 Publication
  1. Vmax=1.2 nmol/sec/mg enzyme with benzoic acid (BA) as substrate (at pH7, in the presence of NAD+)1 Publication
  2. Vmax=3.2 nmol/sec/mg enzyme with NAD+ as substrate (at pH7, in the presence of benzoic acid (BA))1 Publication
  3. Vmax=1.5 nmol/sec/mg enzyme with indole-3-acetaldehyde as substrate (at pH7)1 Publication
  4. Vmax=5.5 nmol/sec/mg enzyme with cinnamylaldehyde as substrate (at pH7)1 Publication

pH dependencei

Optimum pH is 7.1 Publication

Temperature dependencei

Optimum temperature is 30 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi43Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi48Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi51Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • abscisic acid biosynthetic process Source: UniProtKB-KW
  • auxin biosynthetic process Source: UniProtKB-KW
  • glucosinolate metabolic process Source: TAIR

Keywordsi

Molecular functionOxidoreductase
Biological processAbscisic acid biosynthesis, Auxin biosynthesis
Ligand2Fe-2S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding, Molybdenum, NAD

Enzyme and pathway databases

BRENDAi1.2.3.1 399
ReactomeiR-ATH-964975 Vitamins B6 activation to pyridoxal phosphate

Names & Taxonomyi

Protein namesi
Recommended name:
Benzaldehyde dehydrogenase (NAD(+)) (EC:1.2.1.28)
Alternative name(s):
Aldehyde oxidase 4
Short name:
AO-4
Short name:
AtAO-4
Short name:
AtAO2
Indole-3-acetaldehyde oxidase (EC:1.2.3.7)
Short name:
IAA oxidase
Gene namesi
Name:AAO4
Synonyms:AO2
Ordered Locus Names:At1g04580
ORF Names:T1G11.17
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G04580
TAIRilocus:2197798 AT1G04580

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Plants have normal abscisic acid (ABA) levels, normal germination and are not affected in abscisic aldehyde oxidase activity in siliques, dry seeds and leaves. Reduced levels of benzoic acid (BA), 3-benzoyloxypropylglucosinolate and 4-benzoyloxybutylglucosinolate in seeds.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001661121 – 1337Benzaldehyde dehydrogenase (NAD(+))Add BLAST1337

Proteomic databases

PaxDbiQ7G191
PRIDEiQ7G191

PTM databases

iPTMnetiQ7G191

Expressioni

Tissue specificityi

Transcripts expressed at high levels in developing siliques and at low levels in dry seeds.2 Publications

Inductioni

Induced by dehydration, in rosette but not in roots.1 Publication

Gene expression databases

ExpressionAtlasiQ7G191 baseline and differential
GenevisibleiQ7G191 AT

Interactioni

Subunit structurei

Aldehyde oxidases (AO) are homodimers and heterodimers of AO subunits.By similarity

Protein-protein interaction databases

STRINGi3702.AT1G04580.1

Structurei

3D structure databases

ProteinModelPortaliQ7G191
SMRiQ7G191
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 912Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST88
Domaini225 – 409FAD-binding PCMH-typePROSITE-ProRule annotationAdd BLAST185

Sequence similaritiesi

Belongs to the xanthine dehydrogenase family.Curated

Phylogenomic databases

eggNOGiKOG0430 Eukaryota
COG4630 LUCA
COG4631 LUCA
HOGENOMiHOG000191197
InParanoidiQ7G191
KOiK22417
OMAiPYINAAF
OrthoDBiEOG093600DW
PhylomeDBiQ7G191

Family and domain databases

CDDicd00207 fer2, 1 hit
InterProiView protein in InterPro
IPR002888 2Fe-2S-bd
IPR036884 2Fe-2S-bd_dom_sf
IPR036010 2Fe-2S_ferredoxin-like_sf
IPR001041 2Fe-2S_ferredoxin-type
IPR006058 2Fe2S_fd_BS
IPR000674 Ald_Oxase/Xan_DH_a/b
IPR036856 Ald_Oxase/Xan_DH_a/b_sf
IPR016208 Ald_Oxase/xanthine_DH
IPR008274 AldOxase/xan_DH_Mopterin-bd
IPR037165 AldOxase/xan_DH_Mopterin-bd_sf
IPR005107 CO_DH_flav_C
IPR036683 CO_DH_flav_C_dom_sf
IPR016166 FAD-bd_2
IPR036318 FAD-bd_2-like_sf
IPR002346 Mopterin_DH_FAD-bd
PfamiView protein in Pfam
PF01315 Ald_Xan_dh_C, 1 hit
PF02738 Ald_Xan_dh_C2, 1 hit
PF03450 CO_deh_flav_C, 1 hit
PF00941 FAD_binding_5, 1 hit
PF00111 Fer2, 1 hit
PF01799 Fer2_2, 1 hit
PIRSFiPIRSF000127 Xanthine_DH, 1 hit
SMARTiView protein in SMART
SM01008 Ald_Xan_dh_C, 1 hit
SM01092 CO_deh_flav_C, 1 hit
SUPFAMiSSF47741 SSF47741, 1 hit
SSF54292 SSF54292, 1 hit
SSF54665 SSF54665, 1 hit
SSF55447 SSF55447, 1 hit
SSF56003 SSF56003, 1 hit
SSF56176 SSF56176, 1 hit
PROSITEiView protein in PROSITE
PS00197 2FE2S_FER_1, 1 hit
PS51085 2FE2S_FER_2, 1 hit
PS51387 FAD_PCMH, 1 hit

Sequencei

Sequence statusi: Complete.

Q7G191-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGDDLVFAV NGEKFEVLSV NPSTTLLEFL RSNTCFKSVK LSCGEGGCGA
60 70 80 90 100
CIVILSKYDP VLDQVEEYSI NSCLTLLCSL NGCSITTSDG LGNTEKGFHP
110 120 130 140 150
IHKRFAGFHA SQCGFCTPGM CISLYSALSK AHNSQSSPDY LTALAAEKSI
160 170 180 190 200
AGNLCRCTGY RPIADACKSF ASDVDIEDLG FNSFWRKGES REEMLKKLPP
210 220 230 240 250
YNPEKDLITF PDFLKEKIKC QHNVLDQTRY HWSTPGSVAE LQEILATTNP
260 270 280 290 300
GKDRGLIKLV VGNTGTGYYK EEKQYGRYID ISHIPEMSMI KKDDREIEIG
310 320 330 340 350
AVVTISKVID ALMEENTSAY VFKKIGVHME KVANHFIRNS GSIGGNLVMA
360 370 380 390 400
QSKSFPSDIT TLLLAADASV HMINAGRHEK LRMGEYLVSP PILDTKTVLL
410 420 430 440 450
KVHIPRWIAS STTGLLFETY RAALRPIGSA LPYINAAFLA VVSHDASSSG
460 470 480 490 500
IIVDKCRLAF GSYGGYHSIR AREVEDFLTG KILSHSVLYE AVRLLKGIIV
510 520 530 540 550
PSIDTSYSEY KKSLAVGFLF DFLYPLIESG SWDSEGKHID GHIDPTICLP
560 570 580 590 600
LLSSAQQVFE SKEYHPVGEA IIKFGAEMQA SGEAVYVDDI PSLPHCLHGA
610 620 630 640 650
FIYSTKPLAW IKSVGFSGNV TPIGVLAVIT FKDIPEVGQN IGYITMFGTG
660 670 680 690 700
LLFADEVTIS AGQIIALVVA DTQKHADMAA HLAVVEYDSR NIGTPVLSVE
710 720 730 740 750
DAVKRSSLFE VPPEYQPEPV GDISKGMAEA DRKIRSVELR LGSQYFFYME
760 770 780 790 800
TQTALALPDE DNCLVVYSST QAPEFTQTVI ATCLGIPEHN VRVITRRVGG
810 820 830 840 850
GFGGKAIKSM PVATACALAA KKMQRPVRIY VNRKTDMIMA GGRHPLKITY
860 870 880 890 900
SVGFRSDGKL TALDLNLFID AGSDVDVSLV MPQNIMNSLR KYDWGALSFD
910 920 930 940 950
IKVCKTNLPS RTSLRAPGEV QGSYIAESII ENVASSLKMD VDVVRRINLH
960 970 980 990 1000
TYESLRKFYK QAAGEPDEYT LPLLWDKLEV SADFRRRAES VKEFNRCNIW
1010 1020 1030 1040 1050
RKRGISRVPI IHLVIHRPTP GKVSILNDGS VAVEVAGIEV GQGLWTKVQQ
1060 1070 1080 1090 1100
MVAYGLGMIK CEGSDDLLER IRLLQTDTLS MSQSSYTAGS TTSENCCEAV
1110 1120 1130 1140 1150
RLCCGILVER LRPTMNQILE NARSVTWDML IQQANAQSVD LSARTFYKPE
1160 1170 1180 1190 1200
SSSAEYLNYG VGASEVEVDL VTGRTEIIRS DIIYDCGKSL NPAVDLGQIE
1210 1220 1230 1240 1250
GAFVQGIGFF MYEEYTTNEN GLVNEEGTWD YKIPTIDTIP KQFNVQILNS
1260 1270 1280 1290 1300
GHHKNRVLSS KASGEPPLLV AASVHCATRS AIREARKQYL SWNCIDDDHR
1310 1320 1330
ERCDLGFELP VPATMPVVKQ LCGLESIEKY LEWKTYP
Length:1,337
Mass (Da):147,304
Last modified:April 26, 2005 - v2
Checksum:i8DCF487FA3F7D6B8
GO

Sequence cautioni

The sequence AAB80640 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1257V → I in AAC39511 (PubMed:9655945).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB037271 mRNA Translation: BAA90299.1
AC002376 Genomic DNA Translation: AAB80640.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE27717.1
AF039897 mRNA Translation: AAC39511.1
PIRiD86178
T52051
RefSeqiNP_563711.1, NM_100337.3
UniGeneiAt.465

Genome annotation databases

EnsemblPlantsiAT1G04580.1; AT1G04580.1; AT1G04580
GeneIDi839488
GrameneiAT1G04580.1; AT1G04580.1; AT1G04580
KEGGiath:AT1G04580

Similar proteinsi

Entry informationi

Entry nameiALDO4_ARATH
AccessioniPrimary (citable) accession number: Q7G191
Secondary accession number(s): O23027, O49157, Q7GB29
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: April 26, 2005
Last modified: April 25, 2018
This is version 114 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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