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Protein

Fimbrin-1

Gene

FIM1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cross-links actin filaments (F-actin) in a calcium independent manner. Induces the formation of actin aggregates. Stabilizes and prevents F-actin depolymerization mediated by profilin. Key regulator of actin cytoarchitecture, probably involved in cell cycle, cell division, cell elongation and cytoplasmic tractus.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi33 – 44Sequence analysisAdd BLAST12

GO - Molecular functioni

GO - Biological processi

  • actin crosslink formation Source: GO_Central
  • actin filament bundle assembly Source: GO_Central
  • actin filament network formation Source: TAIR
  • circadian rhythm Source: TAIR
Complete GO annotation...

Keywords - Ligandi

Actin-binding, Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Fimbrin-11 Publication
Short name:
AtFIM1
Alternative name(s):
Fimbrin1Curated
Gene namesi
Name:FIM11 Publication
Ordered Locus Names:At4g26700Imported
ORF Names:F10M23.40Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G26700.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000737531 – 687Fimbrin-1Add BLAST687

Proteomic databases

PaxDbiQ7G188.

PTM databases

iPTMnetiQ7G188.

Expressioni

Tissue specificityi

Constitutively expressed in roots, leaves, flowers and siliques, and to lower extent in stems.1 Publication

Gene expression databases

ExpressionAtlasiQ7G188. baseline and differential.
GenevisibleiQ7G188. AT.

Interactioni

Subunit structurei

Interacts with F-actin.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi14064. 1 interactor.
STRINGi3702.AT4G26700.1.

Structurei

Secondary structure

1687
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi126 – 137Combined sources12
Turni141 – 146Combined sources6
Helixi155 – 159Combined sources5
Helixi164 – 173Combined sources10
Helixi180 – 182Combined sources3
Helixi191 – 207Combined sources17
Helixi217 – 222Combined sources6
Helixi225 – 241Combined sources17
Beta strandi242 – 246Combined sources5
Turni262 – 264Combined sources3
Helixi270 – 284Combined sources15
Turni296 – 300Combined sources5
Helixi302 – 311Combined sources10
Helixi313 – 315Combined sources3
Helixi318 – 322Combined sources5
Helixi326 – 339Combined sources14
Helixi348 – 352Combined sources5
Helixi356 – 369Combined sources14
Helixi390 – 402Combined sources13
Turni403 – 406Combined sources4
Helixi414 – 417Combined sources4
Turni418 – 420Combined sources3
Helixi422 – 431Combined sources10
Helixi438 – 440Combined sources3
Helixi449 – 465Combined sources17
Helixi475 – 479Combined sources5
Helixi483 – 503Combined sources21
Helixi516 – 528Combined sources13
Turni529 – 531Combined sources3
Helixi542 – 546Combined sources5
Helixi548 – 557Combined sources10
Helixi559 – 561Combined sources3
Helixi573 – 590Combined sources18
Helixi598 – 602Combined sources5
Helixi606 – 620Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PXYX-ray2.40A/B123-623[»]
ProteinModelPortaliQ7G188.
SMRiQ7G188.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7G188.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 55EF-hand 1PROSITE-ProRule annotationAdd BLAST49
Domaini59 – 94EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini123 – 371Actin-binding 1PROSITE-ProRule annotationAdd BLAST249
Domaini123 – 240CH 1PROSITE-ProRule annotationAdd BLAST118
Domaini268 – 371CH 2PROSITE-ProRule annotationAdd BLAST104
Domaini392 – 621Actin-binding 2PROSITE-ProRule annotationAdd BLAST230
Domaini392 – 498CH 3PROSITE-ProRule annotationAdd BLAST107
Domaini513 – 621CH 4PROSITE-ProRule annotationAdd BLAST109

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi620 – 662Ser-richSequence analysisAdd BLAST43
Compositional biasi637 – 644Thr-richSequence analysis8

Domaini

The actin-binding domain 1 (also called ABD1) seems to not bind F-actin alone while the second actin-binding domain (ABD2) can bind F-actin alone. EF-hand domain and the last 65 C-terminal amino acids are not required to cross-link F-actin, but enhance the stability of the binding.2 Publications

Sequence similaritiesi

Contains 2 actin-binding domains.PROSITE-ProRule annotation
Contains 4 CH (calponin-homology) domains.PROSITE-ProRule annotation
Contains 2 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0046. Eukaryota.
COG5069. LUCA.
HOGENOMiHOG000213447.
InParanoidiQ7G188.
OMAiISMARRC.
OrthoDBiEOG0936047N.
PhylomeDBiQ7G188.

Family and domain databases

CDDicd00014. CH. 3 hits.
Gene3Di1.10.238.10. 1 hit.
1.10.418.10. 4 hits.
InterProiIPR001589. Actinin_actin-bd_CS.
IPR001715. CH-domain.
IPR011992. EF-hand-dom_pair.
[Graphical view]
PfamiPF00307. CH. 4 hits.
[Graphical view]
SMARTiSM00033. CH. 4 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF47576. SSF47576. 1 hit.
PROSITEiPS00020. ACTININ_2. 2 hits.
PS50021. CH. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q7G188-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGYVGVVVS DPWLQSQFTQ VELRTLNSKY VSVKNQNGKV TIEDLPPLFA
60 70 80 90 100
KLKALSATFK EDEIKGMLGE LGSDTSTDVS FEEFLKIYLN LLSKAAEKSG
110 120 130 140 150
GHHKNSSSFL KACTTTLLHT IYQSEKGPFV QHINRYLGDD PFLKQFLPLD
160 170 180 190 200
PHSNQLYELV KDGVLLCKLI NVAVPGTIDE RAINTKRVLN PWERNENHTL
210 220 230 240 250
CLNSAKAVGC SVVNIGTQDL AEGRPHLVLG LISQLIKIQV LADLNLKKTP
260 270 280 290 300
QLVELLEDSD DVEELLRLPP EKVLLKWMNF HLKKGGYKKT VSNFSADLKD
310 320 330 340 350
AQAYAFLLNV LAPEHCDPAT LDAKDPLERA ELVLSHAERM NCKRYLTAEE
360 370 380 390 400
IVEGSSTLNL AFVAQIFHER NGLNKDGKYA FAEMMTEDVE TCRDERCYRL
410 420 430 440 450
WINSLGIDSY VNNVFEDVRN GWILLEVLDK VSPSSVNWKH ASKPPIKMPF
460 470 480 490 500
RKVENCNQVI KIGKQLKFSL VNVAGNDIVQ GNKKLILGLL WQLMRFHMLQ
510 520 530 540 550
LLKSLRSRTL GKEMTDADIL SWANRKVRTM GRKLQIESFK DKSLSSGLFF
560 570 580 590 600
LNLLWAVEPR VVNWNLVTKG ETDDEKRLNA TYIVSVARKL GCSVFLLPED
610 620 630 640 650
IVEVNQKMIL ILTASIMYWS LQRHSRESSD SSSTQSTTTT CTSTASSPAP
660 670 680
SVTEEEEVSS LSGEVTSLAV GDAVSEITTV SEEASIE
Length:687
Mass (Da):76,928
Last modified:February 15, 2005 - v2
Checksum:i4190E4BBDCF55875
GO

Sequence cautioni

The sequence AAB97846 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti120T → A in AAC39359 (PubMed:9484459).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66424 mRNA. Translation: AAC39359.1.
AL035440 Genomic DNA. Translation: CAB36516.1.
AL161565 Genomic DNA. Translation: CAB79525.1.
CP002687 Genomic DNA. Translation: AEE85239.1.
CP002687 Genomic DNA. Translation: AEE85240.1.
AF042668 mRNA. Translation: AAB97843.1.
AF042670 Genomic DNA. Translation: AAB97846.1. Sequence problems.
PIRiT04793.
RefSeqiNP_001031726.1. NM_001036649.1. [Q7G188-1]
NP_194400.1. NM_118804.5. [Q7G188-1]
UniGeneiAt.10511.
At.73283.

Genome annotation databases

EnsemblPlantsiAT4G26700.1; AT4G26700.1; AT4G26700. [Q7G188-1]
AT4G26700.2; AT4G26700.2; AT4G26700. [Q7G188-1]
GeneIDi828777.
GrameneiAT4G26700.1; AT4G26700.1; AT4G26700.
AT4G26700.2; AT4G26700.2; AT4G26700.
KEGGiath:AT4G26700.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66424 mRNA. Translation: AAC39359.1.
AL035440 Genomic DNA. Translation: CAB36516.1.
AL161565 Genomic DNA. Translation: CAB79525.1.
CP002687 Genomic DNA. Translation: AEE85239.1.
CP002687 Genomic DNA. Translation: AEE85240.1.
AF042668 mRNA. Translation: AAB97843.1.
AF042670 Genomic DNA. Translation: AAB97846.1. Sequence problems.
PIRiT04793.
RefSeqiNP_001031726.1. NM_001036649.1. [Q7G188-1]
NP_194400.1. NM_118804.5. [Q7G188-1]
UniGeneiAt.10511.
At.73283.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PXYX-ray2.40A/B123-623[»]
ProteinModelPortaliQ7G188.
SMRiQ7G188.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi14064. 1 interactor.
STRINGi3702.AT4G26700.1.

PTM databases

iPTMnetiQ7G188.

Proteomic databases

PaxDbiQ7G188.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G26700.1; AT4G26700.1; AT4G26700. [Q7G188-1]
AT4G26700.2; AT4G26700.2; AT4G26700. [Q7G188-1]
GeneIDi828777.
GrameneiAT4G26700.1; AT4G26700.1; AT4G26700.
AT4G26700.2; AT4G26700.2; AT4G26700.
KEGGiath:AT4G26700.

Organism-specific databases

TAIRiAT4G26700.

Phylogenomic databases

eggNOGiKOG0046. Eukaryota.
COG5069. LUCA.
HOGENOMiHOG000213447.
InParanoidiQ7G188.
OMAiISMARRC.
OrthoDBiEOG0936047N.
PhylomeDBiQ7G188.

Miscellaneous databases

EvolutionaryTraceiQ7G188.
PROiQ7G188.

Gene expression databases

ExpressionAtlasiQ7G188. baseline and differential.
GenevisibleiQ7G188. AT.

Family and domain databases

CDDicd00014. CH. 3 hits.
Gene3Di1.10.238.10. 1 hit.
1.10.418.10. 4 hits.
InterProiIPR001589. Actinin_actin-bd_CS.
IPR001715. CH-domain.
IPR011992. EF-hand-dom_pair.
[Graphical view]
PfamiPF00307. CH. 4 hits.
[Graphical view]
SMARTiSM00033. CH. 4 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF47576. SSF47576. 1 hit.
PROSITEiPS00020. ACTININ_2. 2 hits.
PS50021. CH. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFIMB1_ARATH
AccessioniPrimary (citable) accession number: Q7G188
Secondary accession number(s): O49182, O49963, Q9SZ12
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: February 15, 2005
Last modified: November 30, 2016
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Cross-links actin with a constant of dissociation of 0.55 µM and stoichiometry of 4 molecules of F-actin for one molecule of FIM1.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.