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Protein

Fimbrin-1

Gene

FIM1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cross-links actin filaments (F-actin) in a calcium independent manner. Induces the formation of actin aggregates. Stabilizes and prevents F-actin depolymerization mediated by profilin. Key regulator of actin cytoarchitecture, probably involved in cell cycle, cell division, cell elongation and cytoplasmic tractus.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi33 – 4412Sequence analysisAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • circadian rhythm Source: TAIR
Complete GO annotation...

Keywords - Ligandi

Actin-binding, Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Fimbrin-11 Publication
Short name:
AtFIM1
Alternative name(s):
Fimbrin1Curated
Gene namesi
Name:FIM11 Publication
Ordered Locus Names:At4g26700Imported
ORF Names:F10M23.40Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G26700.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 687687Fimbrin-1PRO_0000073753Add
BLAST

Proteomic databases

PaxDbiQ7G188.
PRIDEiQ7G188.

PTM databases

iPTMnetiQ7G188.

Expressioni

Tissue specificityi

Constitutively expressed in roots, leaves, flowers and siliques, and to lower extent in stems.1 Publication

Gene expression databases

ExpressionAtlasiQ7G188. baseline and differential.
GenevisibleiQ7G188. AT.

Interactioni

Subunit structurei

Interacts with F-actin.1 Publication

Protein-protein interaction databases

BioGridi14064. 1 interaction.
STRINGi3702.AT4G26700.1.

Structurei

Secondary structure

1
687
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi126 – 13712Combined sources
Turni141 – 1466Combined sources
Helixi155 – 1595Combined sources
Helixi164 – 17310Combined sources
Helixi180 – 1823Combined sources
Helixi191 – 20717Combined sources
Helixi217 – 2226Combined sources
Helixi225 – 24117Combined sources
Beta strandi242 – 2465Combined sources
Turni262 – 2643Combined sources
Helixi270 – 28415Combined sources
Turni296 – 3005Combined sources
Helixi302 – 31110Combined sources
Helixi313 – 3153Combined sources
Helixi318 – 3225Combined sources
Helixi326 – 33914Combined sources
Helixi348 – 3525Combined sources
Helixi356 – 36914Combined sources
Helixi390 – 40213Combined sources
Turni403 – 4064Combined sources
Helixi414 – 4174Combined sources
Turni418 – 4203Combined sources
Helixi422 – 43110Combined sources
Helixi438 – 4403Combined sources
Helixi449 – 46517Combined sources
Helixi475 – 4795Combined sources
Helixi483 – 50321Combined sources
Helixi516 – 52813Combined sources
Turni529 – 5313Combined sources
Helixi542 – 5465Combined sources
Helixi548 – 55710Combined sources
Helixi559 – 5613Combined sources
Helixi573 – 59018Combined sources
Helixi598 – 6025Combined sources
Helixi606 – 62015Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PXYX-ray2.40A/B123-623[»]
ProteinModelPortaliQ7G188.
SMRiQ7G188. Positions 123-623.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7G188.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 5549EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini59 – 9436EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini123 – 371249Actin-binding 1PROSITE-ProRule annotationAdd
BLAST
Domaini123 – 240118CH 1PROSITE-ProRule annotationAdd
BLAST
Domaini268 – 371104CH 2PROSITE-ProRule annotationAdd
BLAST
Domaini392 – 621230Actin-binding 2PROSITE-ProRule annotationAdd
BLAST
Domaini392 – 498107CH 3PROSITE-ProRule annotationAdd
BLAST
Domaini513 – 621109CH 4PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi620 – 66243Ser-richSequence analysisAdd
BLAST
Compositional biasi637 – 6448Thr-richSequence analysis

Domaini

The actin-binding domain 1 (also called ABD1) seems to not bind F-actin alone while the second actin-binding domain (ABD2) can bind F-actin alone. EF-hand domain and the last 65 C-terminal amino acids are not required to cross-link F-actin, but enhance the stability of the binding.2 Publications

Sequence similaritiesi

Contains 2 actin-binding domains.PROSITE-ProRule annotation
Contains 4 CH (calponin-homology) domains.PROSITE-ProRule annotation
Contains 2 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0046. Eukaryota.
COG5069. LUCA.
HOGENOMiHOG000213447.
InParanoidiQ7G188.
OMAiISMARRC.
OrthoDBiEOG0936047N.
PhylomeDBiQ7G188.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
1.10.418.10. 4 hits.
InterProiIPR001589. Actinin_actin-bd_CS.
IPR001715. CH-domain.
IPR011992. EF-hand-dom_pair.
[Graphical view]
PfamiPF00307. CH. 4 hits.
[Graphical view]
SMARTiSM00033. CH. 4 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF47576. SSF47576. 1 hit.
PROSITEiPS00020. ACTININ_2. 2 hits.
PS50021. CH. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q7G188-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGYVGVVVS DPWLQSQFTQ VELRTLNSKY VSVKNQNGKV TIEDLPPLFA
60 70 80 90 100
KLKALSATFK EDEIKGMLGE LGSDTSTDVS FEEFLKIYLN LLSKAAEKSG
110 120 130 140 150
GHHKNSSSFL KACTTTLLHT IYQSEKGPFV QHINRYLGDD PFLKQFLPLD
160 170 180 190 200
PHSNQLYELV KDGVLLCKLI NVAVPGTIDE RAINTKRVLN PWERNENHTL
210 220 230 240 250
CLNSAKAVGC SVVNIGTQDL AEGRPHLVLG LISQLIKIQV LADLNLKKTP
260 270 280 290 300
QLVELLEDSD DVEELLRLPP EKVLLKWMNF HLKKGGYKKT VSNFSADLKD
310 320 330 340 350
AQAYAFLLNV LAPEHCDPAT LDAKDPLERA ELVLSHAERM NCKRYLTAEE
360 370 380 390 400
IVEGSSTLNL AFVAQIFHER NGLNKDGKYA FAEMMTEDVE TCRDERCYRL
410 420 430 440 450
WINSLGIDSY VNNVFEDVRN GWILLEVLDK VSPSSVNWKH ASKPPIKMPF
460 470 480 490 500
RKVENCNQVI KIGKQLKFSL VNVAGNDIVQ GNKKLILGLL WQLMRFHMLQ
510 520 530 540 550
LLKSLRSRTL GKEMTDADIL SWANRKVRTM GRKLQIESFK DKSLSSGLFF
560 570 580 590 600
LNLLWAVEPR VVNWNLVTKG ETDDEKRLNA TYIVSVARKL GCSVFLLPED
610 620 630 640 650
IVEVNQKMIL ILTASIMYWS LQRHSRESSD SSSTQSTTTT CTSTASSPAP
660 670 680
SVTEEEEVSS LSGEVTSLAV GDAVSEITTV SEEASIE
Length:687
Mass (Da):76,928
Last modified:February 15, 2005 - v2
Checksum:i4190E4BBDCF55875
GO

Sequence cautioni

The sequence AAB97846 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti120 – 1201T → A in AAC39359 (PubMed:9484459).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66424 mRNA. Translation: AAC39359.1.
AL035440 Genomic DNA. Translation: CAB36516.1.
AL161565 Genomic DNA. Translation: CAB79525.1.
CP002687 Genomic DNA. Translation: AEE85239.1.
CP002687 Genomic DNA. Translation: AEE85240.1.
AF042668 mRNA. Translation: AAB97843.1.
AF042670 Genomic DNA. Translation: AAB97846.1. Sequence problems.
PIRiT04793.
RefSeqiNP_001031726.1. NM_001036649.1. [Q7G188-1]
NP_194400.1. NM_118804.4. [Q7G188-1]
UniGeneiAt.10511.
At.73283.

Genome annotation databases

EnsemblPlantsiAT4G26700.1; AT4G26700.1; AT4G26700. [Q7G188-1]
AT4G26700.2; AT4G26700.2; AT4G26700. [Q7G188-1]
GeneIDi828777.
KEGGiath:AT4G26700.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66424 mRNA. Translation: AAC39359.1.
AL035440 Genomic DNA. Translation: CAB36516.1.
AL161565 Genomic DNA. Translation: CAB79525.1.
CP002687 Genomic DNA. Translation: AEE85239.1.
CP002687 Genomic DNA. Translation: AEE85240.1.
AF042668 mRNA. Translation: AAB97843.1.
AF042670 Genomic DNA. Translation: AAB97846.1. Sequence problems.
PIRiT04793.
RefSeqiNP_001031726.1. NM_001036649.1. [Q7G188-1]
NP_194400.1. NM_118804.4. [Q7G188-1]
UniGeneiAt.10511.
At.73283.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PXYX-ray2.40A/B123-623[»]
ProteinModelPortaliQ7G188.
SMRiQ7G188. Positions 123-623.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi14064. 1 interaction.
STRINGi3702.AT4G26700.1.

PTM databases

iPTMnetiQ7G188.

Proteomic databases

PaxDbiQ7G188.
PRIDEiQ7G188.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G26700.1; AT4G26700.1; AT4G26700. [Q7G188-1]
AT4G26700.2; AT4G26700.2; AT4G26700. [Q7G188-1]
GeneIDi828777.
KEGGiath:AT4G26700.

Organism-specific databases

TAIRiAT4G26700.

Phylogenomic databases

eggNOGiKOG0046. Eukaryota.
COG5069. LUCA.
HOGENOMiHOG000213447.
InParanoidiQ7G188.
OMAiISMARRC.
OrthoDBiEOG0936047N.
PhylomeDBiQ7G188.

Miscellaneous databases

EvolutionaryTraceiQ7G188.
PROiQ7G188.

Gene expression databases

ExpressionAtlasiQ7G188. baseline and differential.
GenevisibleiQ7G188. AT.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
1.10.418.10. 4 hits.
InterProiIPR001589. Actinin_actin-bd_CS.
IPR001715. CH-domain.
IPR011992. EF-hand-dom_pair.
[Graphical view]
PfamiPF00307. CH. 4 hits.
[Graphical view]
SMARTiSM00033. CH. 4 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF47576. SSF47576. 1 hit.
PROSITEiPS00020. ACTININ_2. 2 hits.
PS50021. CH. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFIMB1_ARATH
AccessioniPrimary (citable) accession number: Q7G188
Secondary accession number(s): O49182, O49963, Q9SZ12
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: February 15, 2005
Last modified: September 7, 2016
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Cross-links actin with a constant of dissociation of 0.55 µM and stoichiometry of 4 molecules of F-actin for one molecule of FIM1.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.