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Q7FAS1 (GLO3_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Peroxisomal (S)-2-hydroxy-acid oxidase GLO3

EC=1.1.3.15
Alternative name(s):
Glycolate oxidase 3
Short name=GOX 3
Short name=OsGLO3
Short chain alpha-hydroxy acid oxidase GLO3
Gene names
Name:GLO3
Ordered Locus Names:Os04g0623500, LOC_Os04g53210
ORF Names:OsJ_16217, OSJNBa0053K19.8
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length367 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Photorespiratory enzyme that can exert a strong regulation over photosynthesis, possibly through a feed-back inhibition on Rubisco activase. Not required for oxalate accumulation By similarity.

Catalytic activity

(S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2.

Cofactor

FMN By similarity.

Pathway

Photosynthesis; photorespiration; glycine from 2-phosphoglycolate: step 2/3.

Subunit structure

Homotetramer or homooctamer. Interacts with rice dwarf virus (RDV) P8. This interaction promotes viral P8 relocation to virus factories peripheral to peroxisomes By similarity.

Subcellular location

Peroxisome By similarity.

Sequence similarities

Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.

Contains 1 FMN hydroxy acid dehydrogenase domain.

Ontologies

Keywords
   Biological processGlycolate pathway
Host-virus interaction
Photorespiration
   Cellular componentPeroxisome
   Coding sequence diversityAlternative splicing
   LigandFlavoprotein
FMN
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdefense response signaling pathway, resistance gene-independent

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

defense response to bacterium

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

hydrogen peroxide biosynthetic process

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

modulation by virus of host morphology or physiology

Inferred from sequence or structural similarity. Source: UniProtKB

oxidative photosynthetic carbon pathway

Inferred from electronic annotation. Source: UniProtKB-UniPathway

photorespiration

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of photosynthesis

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentperoxisome

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionFMN binding

Inferred from electronic annotation. Source: InterPro

glycolate oxidase activity

Inferred from sequence or structural similarity. Source: UniProtKB

long-chain-(S)-2-hydroxy-long-chain-acid oxidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

medium-chain-(S)-2-hydroxy-acid oxidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

very-long-chain-(S)-2-hydroxy-acid oxidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q7FAS1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q7FAS1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     306-307: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 367367Peroxisomal (S)-2-hydroxy-acid oxidase GLO3
PRO_0000403413

Regions

Domain1 – 360360FMN hydroxy acid dehydrogenase
Nucleotide binding286 – 31025FMN By similarity
Motif365 – 3673Microbody targeting signal Potential

Sites

Active site2551Proton acceptor By similarity
Binding site251Substrate Potential
Binding site1071FMN By similarity
Binding site1281FMN By similarity
Binding site1301Substrate By similarity
Binding site1561FMN By similarity
Binding site1651Substrate By similarity
Binding site2311FMN By similarity
Binding site2581Substrate Potential

Natural variations

Alternative sequence306 – 3072Missing in isoform 2.
VSP_040390

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: C1F607AE6F6AD6FB

FASTA36740,533
        10         20         30         40         50         60 
MELITNVSEY EQLAKQKLPK MIYDYYASGA EDQWTLKENR EAFSRILFRP RILIDVSRIN 

        70         80         90        100        110        120 
MATNVLGFNI SMPIMIAPSA MQKMAHPEGE LATARAASAA GTIMTLSSWS TSSVEEVNSA 

       130        140        150        160        170        180 
APGIRFFQLY VYKDRNIVRQ LVRRAELAGF KAIALTVDTP RLGRREADIK NRFNLPPHLV 

       190        200        210        220        230        240 
LKNFEALDLG KMDKTNDSGL ASYVASQVDR SLSWTDVKWL QTITSLPILV KGVMTAEDTR 

       250        260        270        280        290        300 
LAVESGAAGI IVSNHGARQL DYVPATISCL EEVVREAKGR LPVFLDGGVR RGTDVFKALA 

       310        320        330        340        350        360 
LGASGVFIGR PVLFSLAVDG EAGVRKVLQM LRDELELTMA LSGCTSLAEI TRNHVITDSD 


RIRRSRL 

« Hide

Isoform 2 [UniParc].

Checksum: 346F50317934DE96
Show »

FASTA36540,287

References

« Hide 'large scale' references
[1]"Sequence and analysis of rice chromosome 4."
Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y., Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q., Zhang L. expand/collapse author list , Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T., Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R., Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G., Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C., Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J., Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J., Li J., Hong G., Xue Y., Han B.
Nature 420:316-320(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[2]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"The rice annotation project database (RAP-DB): 2008 update."
The rice annotation project (RAP)
Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: cv. Nipponbare.
[4]"The genomes of Oryza sativa: a history of duplications."
Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. expand/collapse author list , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[5]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: cv. Nipponbare.
[6]"Inducible antisense suppression of glycolate oxidase reveals its strong regulation over photosynthesis in rice."
Xu H.-W., Zhang J., Zeng J., Jiang L., Liu E., Peng C., He Z.-H., Peng X.-X.
J. Exp. Bot. 60:1799-1809(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL606645 Genomic DNA. Translation: CAE03500.2.
AP008210 Genomic DNA. Translation: BAF15839.1.
CM000141 Genomic DNA. Translation: EEE61716.1.
AK060221 mRNA. Translation: BAG87371.1.
AK068638 mRNA. Translation: BAG91005.1.
RefSeqNP_001053925.1. NM_001060460.1.
UniGeneOs.6585.

3D structure databases

ProteinModelPortalQ7FAS1.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS04T0623500-02; OS04T0623500-02; OS04G0623500. [Q7FAS1-1]
GeneID4337048.
KEGGosa:4337048.

Organism-specific databases

GrameneQ7FAS1.

Phylogenomic databases

eggNOGCOG1304.
HOGENOMHOG000217463.
KOK11517.
OMARIRMARE.
ProtClustDBCLSN2695126.

Enzyme and pathway databases

UniPathwayUPA00951; UER00912.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR013785. Aldolase_TIM.
IPR012133. Alpha-hydoxy_acid_DH_FMN.
IPR000262. FMN-dep_DH.
IPR008259. FMN_hydac_DH_AS.
[Graphical view]
PfamPF01070. FMN_dh. 1 hit.
[Graphical view]
PIRSFPIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITEPS00557. FMN_HYDROXY_ACID_DH_1. 1 hit.
PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLO3_ORYSJ
AccessionPrimary (citable) accession number: Q7FAS1
Secondary accession number(s): B7E4S4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

PATHWAY comments

Index of metabolic and biosynthesis pathways