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Q7FAS1

- GLO3_ORYSJ

UniProt

Q7FAS1 - GLO3_ORYSJ

Protein

Peroxisomal (S)-2-hydroxy-acid oxidase GLO3

Gene

GLO3

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Photorespiratory enzyme that can exert a strong regulation over photosynthesis, possibly through a feed-back inhibition on Rubisco activase. Not required for oxalate accumulation By similarity.By similarity

    Catalytic activityi

    (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2.

    Cofactori

    FMN.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei25 – 251SubstratePROSITE-ProRule annotation
    Binding sitei107 – 1071FMNPROSITE-ProRule annotation
    Binding sitei128 – 1281FMNPROSITE-ProRule annotation
    Binding sitei130 – 1301SubstratePROSITE-ProRule annotation
    Binding sitei156 – 1561FMNPROSITE-ProRule annotation
    Binding sitei165 – 1651SubstratePROSITE-ProRule annotation
    Binding sitei231 – 2311FMNPROSITE-ProRule annotation
    Active sitei255 – 2551Proton acceptorPROSITE-ProRule annotation
    Binding sitei258 – 2581SubstratePROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi286 – 31025FMNPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. FMN binding Source: InterPro
    2. glycolate oxidase activity Source: UniProtKB
    3. long-chain-(S)-2-hydroxy-long-chain-acid oxidase activity Source: UniProtKB-EC
    4. medium-chain-(S)-2-hydroxy-acid oxidase activity Source: UniProtKB-EC
    5. very-long-chain-(S)-2-hydroxy-acid oxidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. defense response signaling pathway, resistance gene-independent Source: EnsemblPlants/Gramene
    2. defense response to bacterium Source: EnsemblPlants/Gramene
    3. hydrogen peroxide biosynthetic process Source: EnsemblPlants/Gramene
    4. modulation by virus of host morphology or physiology Source: UniProtKB
    5. oxidative photosynthetic carbon pathway Source: UniProtKB-UniPathway
    6. photorespiration Source: UniProtKB
    7. regulation of photosynthesis Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Glycolate pathway, Host-virus interaction, Photorespiration

    Keywords - Ligandi

    Flavoprotein, FMN

    Enzyme and pathway databases

    UniPathwayiUPA00951; UER00912.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Peroxisomal (S)-2-hydroxy-acid oxidase GLO3 (EC:1.1.3.15)
    Alternative name(s):
    Glycolate oxidase 3
    Short name:
    GOX 3
    Short name:
    OsGLO3
    Short chain alpha-hydroxy acid oxidase GLO3
    Gene namesi
    Name:GLO3
    Ordered Locus Names:Os04g0623500, LOC_Os04g53210
    ORF Names:OsJ_16217, OSJNBa0053K19.8
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 4

    Organism-specific databases

    GrameneiQ7FAS1.

    Subcellular locationi

    Peroxisome By similarity

    GO - Cellular componenti

    1. peroxisome Source: UniProtKB

    Keywords - Cellular componenti

    Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 367367Peroxisomal (S)-2-hydroxy-acid oxidase GLO3PRO_0000403413Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer or homooctamer. Interacts with rice dwarf virus (RDV) P8. This interaction promotes viral P8 relocation to virus factories peripheral to peroxisomes By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ7FAS1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 360360FMN hydroxy acid dehydrogenasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi365 – 3673Microbody targeting signalSequence Analysis

    Sequence similaritiesi

    Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.PROSITE-ProRule annotation
    Contains 1 FMN hydroxy acid dehydrogenase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1304.
    HOGENOMiHOG000217463.
    KOiK11517.
    OMAiARIAVEY.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR012133. Alpha-hydoxy_acid_DH_FMN.
    IPR000262. FMN-dep_DH.
    IPR008259. FMN_hydac_DH_AS.
    [Graphical view]
    PfamiPF01070. FMN_dh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000138. Al-hdrx_acd_dh. 1 hit.
    PROSITEiPS00557. FMN_HYDROXY_ACID_DH_1. 1 hit.
    PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q7FAS1-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MELITNVSEY EQLAKQKLPK MIYDYYASGA EDQWTLKENR EAFSRILFRP    50
    RILIDVSRIN MATNVLGFNI SMPIMIAPSA MQKMAHPEGE LATARAASAA 100
    GTIMTLSSWS TSSVEEVNSA APGIRFFQLY VYKDRNIVRQ LVRRAELAGF 150
    KAIALTVDTP RLGRREADIK NRFNLPPHLV LKNFEALDLG KMDKTNDSGL 200
    ASYVASQVDR SLSWTDVKWL QTITSLPILV KGVMTAEDTR LAVESGAAGI 250
    IVSNHGARQL DYVPATISCL EEVVREAKGR LPVFLDGGVR RGTDVFKALA 300
    LGASGVFIGR PVLFSLAVDG EAGVRKVLQM LRDELELTMA LSGCTSLAEI 350
    TRNHVITDSD RIRRSRL 367
    Length:367
    Mass (Da):40,533
    Last modified:July 5, 2004 - v1
    Checksum:iC1F607AE6F6AD6FB
    GO
    Isoform 2 (identifier: Q7FAS1-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         306-307: Missing.

    Show »
    Length:365
    Mass (Da):40,287
    Checksum:i346F50317934DE96
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei306 – 3072Missing in isoform 2. 1 PublicationVSP_040390

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL606645 Genomic DNA. Translation: CAE03500.2.
    AP008210 Genomic DNA. Translation: BAF15839.1.
    CM000141 Genomic DNA. Translation: EEE61716.1.
    AK060221 mRNA. Translation: BAG87371.1.
    AK068638 mRNA. Translation: BAG91005.1.
    RefSeqiNP_001053925.1. NM_001060460.1. [Q7FAS1-1]
    UniGeneiOs.6585.

    Genome annotation databases

    EnsemblPlantsiOS04T0623500-02; OS04T0623500-02; OS04G0623500. [Q7FAS1-1]
    GeneIDi4337048.
    KEGGiosa:4337048.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL606645 Genomic DNA. Translation: CAE03500.2 .
    AP008210 Genomic DNA. Translation: BAF15839.1 .
    CM000141 Genomic DNA. Translation: EEE61716.1 .
    AK060221 mRNA. Translation: BAG87371.1 .
    AK068638 mRNA. Translation: BAG91005.1 .
    RefSeqi NP_001053925.1. NM_001060460.1. [Q7FAS1-1 ]
    UniGenei Os.6585.

    3D structure databases

    ProteinModelPortali Q7FAS1.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS04T0623500-02 ; OS04T0623500-02 ; OS04G0623500 . [Q7FAS1-1 ]
    GeneIDi 4337048.
    KEGGi osa:4337048.

    Organism-specific databases

    Gramenei Q7FAS1.

    Phylogenomic databases

    eggNOGi COG1304.
    HOGENOMi HOG000217463.
    KOi K11517.
    OMAi ARIAVEY.

    Enzyme and pathway databases

    UniPathwayi UPA00951 ; UER00912 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR012133. Alpha-hydoxy_acid_DH_FMN.
    IPR000262. FMN-dep_DH.
    IPR008259. FMN_hydac_DH_AS.
    [Graphical view ]
    Pfami PF01070. FMN_dh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000138. Al-hdrx_acd_dh. 1 hit.
    PROSITEi PS00557. FMN_HYDROXY_ACID_DH_1. 1 hit.
    PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    3. "The rice annotation project database (RAP-DB): 2008 update."
      The rice annotation project (RAP)
      Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: cv. Nipponbare.
    4. "The genomes of Oryza sativa: a history of duplications."
      Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.
      , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
      PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    5. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
      The rice full-length cDNA consortium
      Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: cv. Nipponbare.
    6. "Inducible antisense suppression of glycolate oxidase reveals its strong regulation over photosynthesis in rice."
      Xu H.-W., Zhang J., Zeng J., Jiang L., Liu E., Peng C., He Z.-H., Peng X.-X.
      J. Exp. Bot. 60:1799-1809(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY, NOMENCLATURE.

    Entry informationi

    Entry nameiGLO3_ORYSJ
    AccessioniPrimary (citable) accession number: Q7FAS1
    Secondary accession number(s): B7E4S4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 11, 2011
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3