Q7F8S5 (PR2E2_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peroxiredoxin-2E-2, chloroplastic
EC=1.11.1.15

Alternative name(s):
Peroxiredoxin IIE-2
Thioredoxin reductase 2E-2

Gene names

Name:	PRXIIE-2
Ordered Locus Names:	Os02g0192700, LOC_Os02g09940
ORF Names:	P0437H03.112, P0453H10.33

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	225 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin or glutaredoxin system. May be involved in chloroplast redox homeostasis By similarity.
Catalytic activity	2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.
Subunit structure	Monomer By similarity.
Subcellular location	Plastid › chloroplast stroma By similarity.
Post-translational modification	The Cys-111-SH group is the primary site of oxidation by H2O2, and the oxidized Cys-111 (probably Cys-SOH) rapidly reacts with Cys-136-SH to form an intramolecular disulfide. This disulfide is subsequently reduced by thioredoxin to restore the reduced active form of the enzyme By similarity.
Sequence similarities	Belongs to the peroxiredoxin 2 family. Contains 1 thioredoxin domain.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Domain	Redox-active center Transit peptide
Molecular function	Antioxidant Oxidoreductase Peroxidase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	chloroplast stroma Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	peroxidase activity Inferred from electronic annotation. Source: UniProtKB-KW peroxiredoxin activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 42	42	Chloroplast Potential
	Chain	43 – 225	183	Peroxiredoxin-2E-2, chloroplastic		PRO_0000282284
Regions
	Domain	63 – 225	163	Thioredoxin
Sites
	Active site	111	1	Cysteine sulfenic acid (-SOH) intermediate By similarity
Amino acid modifications
	Disulfide bond	111 ↔ 136		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7F8S5 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 9ED90A75988B6CD7	FASTA	225	23,180
	10 20 30 40 50 60 MAAPTAAALS TLSTASVTSG KRFITSSFSL SFSSRPLATG VRAAGARAAR RSAASASTVV 70 80 90 100 110 120 ATIAVGDKLP DATLSYFDPA DGELKTVTVA ELTAGRKAVL FAVPGAFTPT CSQKHLPGFI 130 140 150 160 170 180 EKAGELHAKG VDAIACVSVN DAFVMRAWKE SLGLGDADVL LLSDGNLELT RALGVEMDLS 190 200 210 220 DKPMGLGVRS RRYALLADDG VVKVLNLEEG GAFTTSSAEE MLKAL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed: 18089549] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[3]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[4]	"Proteomic analysis of rice leaf, stem and root tissues during growth course." Nozu Y., Tsugita A., Kamijo K. Proteomics 6:3665-3670(2006) [PubMed: 16758443] [Abstract] Cited for: PROTEIN SEQUENCE [LARGE SCALE ANALYSIS] OF 62-68, MASS SPECTROMETRY. Strain: cv. Nipponbare.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP000366 Genomic DNA. Translation: BAD15391.1. AP005785 Genomic DNA. Translation: BAD34026.1. AP008208 Genomic DNA. Translation: BAF08079.1. AK061629 mRNA. No translation available.
RefSeq	NP_001046165.1. NM_001052700.1.
UniGene	Os.4251.
3D structure databases
HSSP	HSSP built from PDB template 1HD2 based on UniProtKB P30044.
ProteinModelPortal	Q7F8S5.
SMR	Q7F8S5. Positions 61-223.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7F8S5.
Protein family/group databases
PeroxiBase	4019. OsPrxIIE02.
Proteomic databases
PRIDE	Q7F8S5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os02g09940.1; LOC_Os02g09940.1; LOC_Os02g09940.
GeneID	4328586.
KEGG	osa:4328586.
Organism-specific databases
Gramene	Q7F8S5.
Phylogenomic databases
GeneTree	EPGT00070000029465.
HOGENOM	HBG493509.
OMA	KGVDEIW.
PhylomeDB	Q7F8S5.
ProtClustDB	CLSN2720528.
Family and domain databases
InterPro	IPR013740. Redoxin. IPR012336. Thioredoxin-like_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF08534. Redoxin. 1 hit. [Graphical view]
SUPFAM	SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PR2E2_ORYSJ

Accession

Primary (citable) accession number: Q7F8S5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	July 5, 2004
Last modified:	October 19, 2011
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program