Reviewed, UniProtKB/Swiss-Prot Q7F8S5 (PR2E2_ORYSJ)

Names and origin

Protein names

Recommended name:
    Peroxiredoxin-2E-2, chloroplastic
    EC=1.11.1.15
Alternative name(s):
    Peroxiredoxin IIE-2
    Thioredoxin reductase 2E-2

Gene names

Name:	PRXIIE-2
Ordered Locus Names:	Os02g0192700, LOC_Os02g09940
ORF Names:	P0437H03.112, P0453H10.33

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	225 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Reduces hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin or glutaredoxin system. May be involved in chloroplast redox homeostasis By similarity.
Catalytic activity	2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.
Subunit structure	Monomer By similarity.
Subcellular location	Plastid › chloroplast stroma By similarity.
Post-translational modification	The Cys-111-SH group is the primary site of oxidation by H2O2, and the oxidized Cys-111 (probably Cys-SOH) rapidly reacts with Cys-136-SH to form an intramolecular disulfide. This disulfide is subsequently reduced by thioredoxin to restore the reduced active form of the enzyme By similarity.
Sequence similarities	Belongs to the peroxiredoxin 2 family. Contains 1 thioredoxin domain.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Domain	Redox-active center Transit peptide
Molecular function	Antioxidant Oxidoreductase Peroxidase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast stroma Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	peroxiredoxin activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 42	42	Chloroplast Potential
	Chain	43 – 225	183	Peroxiredoxin-2E-2, chloroplastic		PRO_0000282284
Regions
	Domain	63 – 225	163	Thioredoxin
Sites
	Active site	111	1	Cysteine sulfenic acid (-SOH) intermediate By similarity
Amino acid modifications
	Disulfide bond	111 ↔ 136		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7F8S5-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 9ED90A75988B6CD7	FASTA	225	23,180
	10 20 30 40 50 60 MAAPTAAALS TLSTASVTSG KRFITSSFSL SFSSRPLATG VRAAGARAAR RSAASASTVV 70 80 90 100 110 120 ATIAVGDKLP DATLSYFDPA DGELKTVTVA ELTAGRKAVL FAVPGAFTPT CSQKHLPGFI 130 140 150 160 170 180 EKAGELHAKG VDAIACVSVN DAFVMRAWKE SLGLGDADVL LLSDGNLELT RALGVEMDLS 190 200 210 220 DKPMGLGVRS RRYALLADDG VVKVLNLEEG GAFTTSSAEE MLKAL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[3]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[4]	"Proteomic analysis of rice leaf, stem and root tissues during growth course." Nozu Y., Tsugita A., Kamijo K. Proteomics 6:3665-3670(2006) [PubMed: 16758443] [Abstract] Cited for: PROTEIN SEQUENCE [LARGE SCALE ANALYSIS] OF 62-68, MASS SPECTROMETRY. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP000366 Genomic DNA. Translation: BAD15391.1. AP005785 Genomic DNA. Translation: BAD34026.1. AP008208 Genomic DNA. Translation: BAF08079.1. AK061629 mRNA. No translation available.
RefSeq	NP_001046165.1.
UniGene	Os.4251
3D structure databases
ModBase	Search...
Protein family/group databases
PeroxiBase	4019. OsPrxIIE02.
Genome annotation databases
GeneID	4328586.
KEGG	osa:4328586.
Organism-specific databases
Gramene	Q7F8S5.
Family and domain databases
InterPro	IPR013740. Redoxin. IPR017936. Thioredoxin-like. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF08534. Redoxin. 1 hit. [Graphical view]
PROSITE	PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PR2E2_ORYSJ

Accession

Primary (citable) accession number: Q7F8S5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	July 5, 2004
Last modified:	June 16, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)