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Protein

Polyprenol reductase 2

Gene

Os07g0493400

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism (By similarity).By similarity

Catalytic activityi

Ditrans,polycis-dolichol + NADP+ = ditrans,polycis-polyprenol + NADPH.

Pathwayi

GO - Molecular functioni

  1. oxidoreductase activity, acting on the CH-CH group of donors Source: InterPro

GO - Biological processi

  1. lipid metabolic process Source: InterPro
  2. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

ReactomeiREACT_284334. Androgen biosynthesis.
REACT_315607. Synthesis of Dolichyl-phosphate.
UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyprenol reductase 2 (EC:1.3.1.94)
Gene namesi
Ordered Locus Names:Os07g0493400, LOC_Os07g31140
ORF Names:OsJ_24314, P0038F10.126
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763 Componenti: Chromosome 7

Organism-specific databases

GrameneiQ7F0Q2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei11 – 3121HelicalSequence AnalysisAdd
BLAST
Transmembranei78 – 9821HelicalSequence AnalysisAdd
BLAST
Transmembranei175 – 19521HelicalSequence AnalysisAdd
BLAST
Transmembranei234 – 25421HelicalSequence AnalysisAdd
BLAST
Transmembranei291 – 30818HelicalSequence AnalysisAdd
BLAST
Transmembranei313 – 33523HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 353353Polyprenol reductase 2PRO_0000398659Add
BLAST

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi60 – 678Poly-Ser

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG330066.
InParanoidiQ7F0Q2.
OMAiTHQWYRE.

Family and domain databases

InterProiIPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view]
PfamiPF02544. Steroid_dh. 1 hit.
[Graphical view]
PROSITEiPS50244. S5A_REDUCTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7F0Q2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESEGWPALQ PLLCFAWIAA TLPIIAAALP IPTAVGGHLL RRLLSAFSSR
60 70 80 90 100
GKTVRPSPAS SSGSSSSKAK FTVPQKYFMH FYVVGVLATT ILLLAIWFYA
110 120 130 140 150
YMKLTPLLLE SSSYSTIFSH LVGSNSFSFG RVRSRTMGHK YRVWRTVFAL
160 170 180 190 200
LLMEVQVLRR LYETEHVFHY SPARMHIVGY LTGLFYYVAA PLSLASSCIP
210 220 230 240 250
EAAEYFQGQV PEFVVKGRAR MPDLVIDSSS LLQPLLKLGW TQWIGAVIFI
260 270 280 290 300
WGSLHQIRCH AILGSLREHK DYDEYVIPCG DCFNRVSCPH YLAELVIYFG
310 320 330 340 350
MLVASGAEDI PVWFLFIFVI TNLSFAAVET YNWYLQKFED YPRSRYAIIP

FVC
Length:353
Mass (Da):39,935
Last modified:July 5, 2004 - v1
Checksum:i08E6F432E2DCE6C0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP004266 Genomic DNA. Translation: BAC83358.1.
CM000144 Genomic DNA. Translation: EAZ39875.1.
AK241048 mRNA. Translation: BAH00939.1.

Genome annotation databases

EnsemblPlantsiOS07T0493400-01; OS07T0493400-01; OS07G0493400.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP004266 Genomic DNA. Translation: BAC83358.1.
CM000144 Genomic DNA. Translation: EAZ39875.1.
AK241048 mRNA. Translation: BAH00939.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS07T0493400-01; OS07T0493400-01; OS07G0493400.

Organism-specific databases

GrameneiQ7F0Q2.

Phylogenomic databases

eggNOGiNOG330066.
InParanoidiQ7F0Q2.
OMAiTHQWYRE.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiREACT_284334. Androgen biosynthesis.
REACT_315607. Synthesis of Dolichyl-phosphate.

Family and domain databases

InterProiIPR001104. 3-oxo-5_a-steroid_4-DH_C.
[Graphical view]
PfamiPF02544. Steroid_dh. 1 hit.
[Graphical view]
PROSITEiPS50244. S5A_REDUCTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. "The genomes of Oryza sativa: a history of duplications."
    Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.
    , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
    PLoS Biol. 3:266-281(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "Oryza sativa full length cDNA."
    The rice full-length cDNA consortium
    Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Nipponbare.
    Tissue: Callus.

Entry informationi

Entry nameiPOED2_ORYSJ
AccessioniPrimary (citable) accession number: Q7F0Q2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: July 5, 2004
Last modified: April 1, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.