Q7EYV7 (PARP1_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Poly [ADP-ribose] polymerase 1
Short name=PARP-1
EC=2.4.2.30

Alternative name(s):
NAD(+) ADP-ribosyltransferase 1
Short name=ADPRT-1
Poly[ADP-ribose] synthase 1

Gene names

Name:	PARP1
Ordered Locus Names:	Os07g0413700, LOC_Os07g23110
ORF Names:	OSJNBa0077F02.113

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	977 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks By similarity.
Catalytic activity	NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.
Subcellular location	Nucleus Potential.
Sequence similarities	Contains 1 BRCT domain. Contains 1 PARP alpha-helical domain. Contains 1 PARP catalytic domain. Contains 2 PARP-type zinc fingers. Contains 1 SAP domain.
Sequence caution	The sequence AK103479 differs from that shown. Reason: Erroneous termination at position 502. Translated as Gln.

Ontologies

Keywords
Cellular component	Nucleus
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding NAD Zinc
Molecular function	Glycosyltransferase Transferase
PTM	ADP-ribosylation
Gene Ontology (GO)
Biological process	protein ADP-ribosylation Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW NAD binding Inferred from electronic annotation. Source: InterPro NAD+ ADP-ribosyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 977	977	Poly [ADP-ribose] polymerase 1		PRO_0000260502
Regions
	Domain	254 – 288	35	SAP
	Domain	381 – 473	93	BRCT
	Domain	626 – 745	120	PARP alpha-helical
	Domain	752 – 977	226	PARP catalytic
	Zinc finger	8 – 91	84	PARP-type 1
	Zinc finger	104 – 179	76	PARP-type 2
Experimental info
	Sequence conflict	233	1	S → F in AK103479. Ref.3
	Sequence conflict	612	1	G → S in AK103479. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q7EYV7 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 0BBB8B57BFF9976B	FASTA	977	110,156
	10 20 30 40 50 60 MAAPPKAWKA EYAKSGRSSC KSCRSPIGKD QLRLGKMVQA TQFDGLMPMW NHASCILSKK 70 80 90 100 110 120 NQIKSVDDVE GIDTLRWDDQ EKIRNYVGSA PATASSAAAI SDKCTIEVAK SARTSCRRCG 130 140 150 160 170 180 EKIKKGTVRV SSKLEGQGWY HASCFLEMSP AATVENFSGW EILSHEDKRA VLDLVKKDAP 190 200 210 220 230 240 SSGQTSSKGS KRKNNQNDIH DCKAPKIIRS ISEGTAEDKG KAVVSHDSNA NSSDLQEKLK 250 260 270 280 290 300 EQSDTLWKLK DELKKHVSTA ELRNMLEANG QDTSGPERHL LDRCADGMLF GALGTCPVCS 310 320 330 340 350 360 SFLYYHGGQY HCSGYVSEWS KCTYSTTEPV RSKKKWKIPD EMDNGYLTKW FKSQKAKKPE 370 380 390 400 410 420 RVLPPMSPEK SLCQSTQQNR SFLSEGLDKL RVSIVGQSKD VVDGWKQKLK DAGANFNATV 430 440 450 460 470 480 TKDSSCLVLC SELESENAEV KKARRLKIPI LREGYLGECI RKNRVLPFDL YKVEAALESS 490 500 510 520 530 540 KGGTMTVKVK GRSAVHESSG LQDTGHILED GKSIYNTTLN MSDLTRGVNS YYILQVIEED 550 560 570 580 590 600 NGSDCYVFRK WGRVGNEKIG GTKLEEMSKI HAIQEFRRLF LEKTGNPWEA WEQKTNFQKQ 610 620 630 640 650 660 PGKFYPLDID YGVRQGPKRK DIDKMKSSLP PQLLELMNML FNIETYRAAM LEFKINMSEM 670 680 690 700 710 720 PLGKLSKENI QKGFEALTEI QNLLGNTNNQ ELAVRESLIV AASNRFFTLI PSIHPHIIQD 730 740 750 760 770 780 EDDLMVKVKM LEALQDIEIA SKLVGFDSDN DESLDDKYKK LRCAITPLPH DCEDYKLVEK 790 800 810 820 830 840 YLLNTHAPTH KEWSLELEEV FSLDRDGEFS KYSRYKNNLH NKMLLWHGSR LTNYVGILSQ 850 860 870 880 890 900 GLRIAPPEAP VTGYMFGKGL YFADLVSKSA QYCYVDRKNP VGLMLLSEVA LGDMYELKKA 910 920 930 940 950 960 TSMDKPPRGK HSTKGLGKTV PLESEFAKWR DDVVVPCGKP VPASIKTSEL MYNEYIVYNT 970 SQVKMQYLLK VRFHHKR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[2]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed: 18089549] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[3]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed: 12869764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP005247 Genomic DNA. Translation: BAC84104.1. AP008213 Genomic DNA. Translation: BAF21367.1. AK103479 mRNA. No translation available.
RefSeq	NP_001059453.1. NM_001065988.1.
UniGene	Os.31900.
3D structure databases
HSSP	HSSP built from PDB template 1GS0 based on UniProtKB O88554.
ProteinModelPortal	Q7EYV7.
SMR	Q7EYV7. Positions 6-90.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os07g23110.1; LOC_Os07g23110.1; LOC_Os07g23110.
GeneID	4343013.
KEGG	osa:4343013.
Organism-specific databases
Gramene	Q7EYV7.
Phylogenomic databases
GeneTree	EPGT00070000030126.
HOGENOM	HBG356841.
OMA	IKEKNNM.
PhylomeDB	Q7EYV7.
ProtClustDB	PLN03123.
Family and domain databases
InterPro	IPR001357. BRCT. IPR008288. NAD_ADPRT. IPR012982. PADR1. IPR012317. Poly(ADP-ribose)pol_cat_dom. IPR004102. Poly(ADP-ribose)pol_reg_dom. IPR003034. SAP_DNA-bd. IPR008893. WGR_domain. IPR001510. Znf_PARP. [Graphical view]
Gene3D	G3DSA:1.20.142.10. PARP_reg. 1 hit. G3DSA:3.30.1740.10. Znf_PARP. 2 hits.
KO	K10798.
Pfam	PF08063. PADR1. 1 hit. PF00644. PARP. 1 hit. PF02877. PARP_reg. 1 hit. PF05406. WGR. 1 hit. PF00645. zf-PARP. 2 hits. [Graphical view]
PIRSF	PIRSF000489. NAD_ADPRT. 1 hit.
SMART	SM00292. BRCT. 1 hit. SM00773. WGR. 1 hit. [Graphical view]
SUPFAM	SSF52113. BRCT. 1 hit. SSF47587. PARP_reg. 1 hit. SSF142921. SSF142921. 1 hit.
PROSITE	PS50172. BRCT. 1 hit. PS51060. PARP_ALPHA_HD. 1 hit. PS51059. PARP_CATALYTIC. 1 hit. PS50064. PARP_ZN_FINGER_2. 2 hits. PS50800. SAP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PARP1_ORYSJ

Accession

Primary (citable) accession number: Q7EYV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	July 5, 2004
Last modified:	December 14, 2011
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program