Reviewed,
UniProtKB/Swiss-Prot Q7DNA1 (CHI2_ORYSJ)
Last modified
November 24, 2009.
Version 39.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Chitinase 2 EC=3.2.1.14 Alternative name(s): Pathogenesis related (PR)-3 chitinase 2 Class I chitinase b Short name=OsChia1b | ||||||||
| Gene names |
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| Organism | Oryza sativa subsp. japonica (Rice) | ||||||||
| Taxonomic identifier | 39947 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza |
Protein attributes
| Sequence length | 340 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Hydrolyzes chitin and plays a role in defense against fungal pathogens containing chitin. Its overexpression confers enhanced resistance to sheath blight pathogen (R.solani). Ref.5 Ref.7 Ref.8 |
| Catalytic activity | Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins. |
| Tissue specificity | Expressed in roots, sheaths and meristems. Ref.6 |
| Sequence similarities | Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily. Contains 1 chitin-binding type-1 domain. |
| Sequence caution | The sequence BAF17390.1 differs from that shown. Reason: Erroneous gene model prediction. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Carbohydrate metabolism Chitin degradation Plant defense Polysaccharide degradation |
| Domain | Signal |
| Ligand | Chitin-binding |
| Molecular function | Glycosidase Hydrolase |
| PTM | Disulfide bond |
| Technical term | 3D-structure |
| Gene Ontology (GO) | |
| Biological process | cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro chitin catabolic processInferred from electronic annotation. Source: InterPro defense response to fungus Ref.7Inferred from direct assay. Source: UniProtKB |
| Molecular function | chitin binding Inferred from electronic annotation. Source: UniProtKB-KW chitinase activity Ref.7 Ref.8Inferred from direct assay. Source: UniProtKB |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 32 | 32 | Potential | ||||||||
| Chain | 33 – 340 | 308 | Chitinase 2 | PRO_5000139669 | |||||||
Regions | |||||||||||
| Domain | 33 – 73 | 41 | Chitin-binding type-1 | ||||||||
Amino acid modifications | |||||||||||
| Disulfide bond | 35 ↔ 50 | Ref.9 | |||||||||
| Disulfide bond | 44 ↔ 56 | Ref.9 | |||||||||
| Disulfide bond | 47 ↔ 74 | Ref.9 | |||||||||
| Disulfide bond | 49 ↔ 63 | Ref.9 | |||||||||
| Disulfide bond | 67 ↔ 71 | Ref.9 | |||||||||
| Disulfide bond | 110 ↔ 172 | Ref.9 | |||||||||
| Disulfide bond | 184 ↔ 192 | Ref.9 | |||||||||
| Disulfide bond | 291 ↔ 323 | Ref.9 | |||||||||
Experimental info | |||||||||||
| Mutagenesis | 159 | 1 | W → A: Increased activity. Ref.8 | ||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Sequence variation, differential expression and chromosomal location of rice chitinase genes." Nishizawa Y., Kishimoto N., Saito A., Hibi T. Mol. Gen. Genet. 241:1-10(1993) [PubMed: 7901749] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: cv. Nipponbare. |
| [2] | "The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare. |
| [3] | "Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare. |
| [4] | "The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. Yang H.PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare. |
| [5] | "Enhanced resistance to sheath blight by constitutive expression of infection-related rice chitinase in transgenic elite indica rice cultivars." Datta K., Tu J., Oliva N., Ona I., Velazhahan R., Mew T.W., Muthukrishnan S., Datta S.K. Plant Sci. 160:405-414(2001) [PubMed: 11166426] [Abstract] Cited for: FUNCTION. |
| [6] | "Distribution, structure, organ-specific expression, and phylogenic analysis of the pathogenesis-related protein-3 chitinase gene family in rice (Oryza sativa L.)." Nakazaki T., Tsukiyama T., Okumoto Y., Kageyama D., Naito K., Inouye K., Tanisaka T. Genome 49:619-630(2006) [PubMed: 16936841] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE, TISSUE SPECIFICITY. |
| [7] | "Purification and characterization of a rice class I chitinase, OsChia1b, produced in Esherichia coli." Mizuno R., Itoh Y., Nishizawa Y., Kezuka Y., Suzuki K., Nonaka T., Watanabe T. Biosci. Biotechnol. Biochem. 72:893-895(2008) [PubMed: 18323646] [Abstract] Cited for: FUNCTION. |
| [8] | "Role of the loop structure of the catalytic domain in rice class I chitinase." Mizuno R., Fukamizo T., Sugiyama S., Nishizawa Y., Kezuka Y., Nonaka T., Suzuki K., Watanabe T. J. Biochem. 143:487-495(2008) [PubMed: 18211919] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF TRP-159. |
| [9] | "Crystallization and preliminary X-ray analysis of plant class I chitinase from rice." Kezuka Y., Kitazaki K., Itoh Y., Watanabe J., Takaha O., Watanabe T., Nishizawa Y., Nonaka T. Protein Pept. Lett. 11:401-405(2004) [PubMed: 15327374] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 33-340, DISULFIDE BONDS. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| D16222 Genomic DNA. Translation: BAA03750.1. X56787 mRNA. Translation: CAA40107.1. AP008211 Genomic DNA. Translation: BAF17390.1. Sequence problems. CM000142 Genomic DNA. Translation: EEE63650.1. | |||||||||||||
| PIR | S39979. S40414. | ||||||||||||
| RefSeq | NP_001055476.1. | ||||||||||||
3D structure databases | |||||||||||||
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| ModBase | Search... | ||||||||||||
Protein family/group databases | |||||||||||||
| CAZy | CBM18. Carbohydrate-Binding Module Family 18. GH19. Glycoside Hydrolase Family 19. | ||||||||||||
Genome annotation databases | |||||||||||||
| GeneID | 4338718. | ||||||||||||
| GenomeReviews | Gene locus Cht2 in contig AP008211_GR. | ||||||||||||
Organism-specific databases | |||||||||||||
| Gramene | Q7DNA1. | ||||||||||||
Family and domain databases | |||||||||||||
| InterPro | IPR018371. Chitin-binding_1_CS. IPR001002. Chitin_bd_1. IPR016283. Glyco_hydro_19. IPR000726. Glyco_hydro_19_cat. [Graphical view] | ||||||||||||
| Gene3D | G3DSA:3.30.60.10. Chitin_bd_1. 1 hit. | ||||||||||||
| PANTHER | PTHR22595. Glyco_hydro_19_cat. 1 hit. | ||||||||||||
| Pfam | PF00187. Chitin_bind_1. 1 hit. PF00182. Glyco_hydro_19. 1 hit. [Graphical view] | ||||||||||||
| PIRSF | PIRSF001060. Endochitinase. 1 hit. | ||||||||||||
| PRINTS | PR00451. CHITINBINDNG. | ||||||||||||
| ProDom | PD000609. Chitin_bd_1. 1 hit. [Graphical view] [Entries sharing at least one domain] | ||||||||||||
| SMART | SM00270. ChtBD1. 1 hit. [Graphical view] | ||||||||||||
| PROSITE | PS00026. CHIT_BIND_I_1. 1 hit. PS50941. CHIT_BIND_I_2. 1 hit. PS00773. CHITINASE_19_1. 1 hit. PS00774. CHITINASE_19_2. 1 hit. [Graphical view] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Entry information
| Entry name | CHI2_ORYSJ | ||||||||
| Accession | Primary (citable) accession number: Q7DNA1 Secondary accession number(s): Q0DID3, Q43294 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Glycosyl hydrolases Classification of glycosyl hydrolase families and list of entries |
| Oryza sativa (rice) Index of Oryza sativa entries and their corresponding gene designations |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

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