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Q7DNA1

- CHI2_ORYSJ

UniProt

Q7DNA1 - CHI2_ORYSJ

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Protein
Chitinase 2
Gene
Cht2, RC7, Os05g0399300, LOC_Os05g33130, OsJ_18467
Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Hydrolyzes chitin and plays a role in defense against fungal pathogens containing chitin. Its overexpression confers enhanced resistance to sheath blight pathogen (R.solani).3 Publications

Catalytic activityi

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

GO - Molecular functioni

  1. chitin binding Source: UniProtKB-KW
  2. chitinase activity Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. cell wall macromolecule catabolic process Source: InterPro
  2. chitin catabolic process Source: UniProtKB-KW
  3. defense response to fungus Source: UniProtKB
  4. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Chitin degradation, Plant defense, Polysaccharide degradation

Keywords - Ligandi

Chitin-binding

Protein family/group databases

CAZyiCBM18. Carbohydrate-Binding Module Family 18.
GH19. Glycoside Hydrolase Family 19.

Names & Taxonomyi

Protein namesi
Recommended name:
Chitinase 2 (EC:3.2.1.14)
Alternative name(s):
Class I chitinase b
Short name:
OsChia1b
Pathogenesis related (PR)-3 chitinase 2
Gene namesi
Name:Cht2
Synonyms:RC7
Ordered Locus Names:Os05g0399300, LOC_Os05g33130
ORF Names:OsJ_18467
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 5

Organism-specific databases

GrameneiQ7DNA1.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi159 – 1591W → A: Increased activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232 Reviewed prediction
Add
BLAST
Chaini33 – 340308Chitinase 2
PRO_5000139669Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi35 ↔ 501 Publication
Disulfide bondi44 ↔ 561 Publication
Disulfide bondi47 ↔ 741 Publication
Disulfide bondi49 ↔ 631 Publication
Disulfide bondi67 ↔ 711 Publication
Disulfide bondi110 ↔ 1721 Publication
Disulfide bondi184 ↔ 1921 Publication
Disulfide bondi291 ↔ 3231 Publication

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed in roots, sheaths and meristems.1 Publication

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni37 – 415
Helixi45 – 473
Beta strandi54 – 596
Helixi60 – 634
Helixi89 – 913
Helixi95 – 1017
Turni102 – 1065
Turni111 – 1144
Helixi118 – 1269
Turni129 – 1324
Beta strandi134 – 1363
Helixi137 – 15519
Helixi166 – 1683
Beta strandi188 – 1903
Turni202 – 2054
Helixi209 – 21911
Turni223 – 2253
Helixi229 – 2324
Helixi234 – 24613
Helixi255 – 2595
Helixi267 – 2715
Helixi278 – 29013
Beta strandi291 – 2944
Helixi297 – 31216

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DKVX-ray2.00A33-340[»]
3IWRX-ray2.57A/B33-340[»]
ProteinModelPortaliQ7DNA1.

Miscellaneous databases

EvolutionaryTraceiQ7DNA1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini33 – 7341Chitin-binding type-1
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000231411.
KOiK01183.
OMAiNCLCCSR.

Family and domain databases

Gene3Di3.30.60.10. 1 hit.
InterProiIPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamiPF00187. Chitin_bind_1. 1 hit.
PF00182. Glyco_hydro_19. 1 hit.
[Graphical view]
PIRSFiPIRSF001060. Endochitinase. 1 hit.
PRINTSiPR00451. CHITINBINDNG.
ProDomiPD000609. Chitin_bd_1. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00270. ChtBD1. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEiPS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7DNA1-1 [UniParc]FASTAAdd to Basket

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MSTPRAAASL AKKAALVALA VLAAALATAA RAEQCGAQAG GARCPNCLCC    50
SRWGWCGTTS DFCGDGCQSQ CSGCGPTPTP TPPSPSDGVG SIVPRDLFER 100
LLLHRNDGAC PARGFYTYEA FLAAAAAFPA FGGTGNTETR KREVAAFLGQ 150
TSHETTGGWP TAPDGPFSWG YCFKQEQNPP SDYCQPSPEW PCAPGRKYYG 200
RGPIQLSFNF NYGPAGRAIG VDLLSNPDLV ATDATVSFKT ALWFWMTPQG 250
NKPSSHDVIT GRWAPSPADA AAGRAPGYGV ITNIVNGGLE CGHGPDDRVA 300
NRIGFYQRYC GAFGIGTGGN LDCYNQRPFN SGSSVGLAEQ 340
Length:340
Mass (Da):35,587
Last modified:July 5, 2004 - v1
Checksum:i642F13E3928CA7BE
GO

Sequence cautioni

The sequence BAF17390.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D16222 Genomic DNA. Translation: BAA03750.1.
X56787 mRNA. Translation: CAA40107.1.
AP008211 Genomic DNA. Translation: BAF17390.1. Sequence problems.
CM000142 Genomic DNA. Translation: EEE63650.1.
PIRiS39979.
S40414.
RefSeqiNP_001055476.1. NM_001062011.1.
UniGeneiOs.3374.

Genome annotation databases

GeneIDi4338718.
KEGGiosa:4338718.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D16222 Genomic DNA. Translation: BAA03750.1 .
X56787 mRNA. Translation: CAA40107.1 .
AP008211 Genomic DNA. Translation: BAF17390.1 . Sequence problems.
CM000142 Genomic DNA. Translation: EEE63650.1 .
PIRi S39979.
S40414.
RefSeqi NP_001055476.1. NM_001062011.1.
UniGenei Os.3374.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2DKV X-ray 2.00 A 33-340 [» ]
3IWR X-ray 2.57 A/B 33-340 [» ]
ProteinModelPortali Q7DNA1.
ModBasei Search...

Protein family/group databases

CAZyi CBM18. Carbohydrate-Binding Module Family 18.
GH19. Glycoside Hydrolase Family 19.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 4338718.
KEGGi osa:4338718.

Organism-specific databases

Gramenei Q7DNA1.

Phylogenomic databases

HOGENOMi HOG000231411.
KOi K01183.
OMAi NCLCCSR.

Miscellaneous databases

EvolutionaryTracei Q7DNA1.

Family and domain databases

Gene3Di 3.30.60.10. 1 hit.
InterProi IPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view ]
Pfami PF00187. Chitin_bind_1. 1 hit.
PF00182. Glyco_hydro_19. 1 hit.
[Graphical view ]
PIRSFi PIRSF001060. Endochitinase. 1 hit.
PRINTSi PR00451. CHITINBINDNG.
ProDomi PD000609. Chitin_bd_1. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SMARTi SM00270. ChtBD1. 1 hit.
[Graphical view ]
SUPFAMi SSF53955. SSF53955. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEi PS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence variation, differential expression and chromosomal location of rice chitinase genes."
    Nishizawa Y., Kishimoto N., Saito A., Hibi T.
    Mol. Gen. Genet. 241:1-10(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: cv. Nipponbare.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  4. "The genomes of Oryza sativa: a history of duplications."
    Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.
    , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
    PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  5. "Enhanced resistance to sheath blight by constitutive expression of infection-related rice chitinase in transgenic elite indica rice cultivars."
    Datta K., Tu J., Oliva N., Ona I., Velazhahan R., Mew T.W., Muthukrishnan S., Datta S.K.
    Plant Sci. 160:405-414(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Distribution, structure, organ-specific expression, and phylogenic analysis of the pathogenesis-related protein-3 chitinase gene family in rice (Oryza sativa L.)."
    Nakazaki T., Tsukiyama T., Okumoto Y., Kageyama D., Naito K., Inouye K., Tanisaka T.
    Genome 49:619-630(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE, TISSUE SPECIFICITY.
  7. "Purification and characterization of a rice class I chitinase, OsChia1b, produced in Esherichia coli."
    Mizuno R., Itoh Y., Nishizawa Y., Kezuka Y., Suzuki K., Nonaka T., Watanabe T.
    Biosci. Biotechnol. Biochem. 72:893-895(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Role of the loop structure of the catalytic domain in rice class I chitinase."
    Mizuno R., Fukamizo T., Sugiyama S., Nishizawa Y., Kezuka Y., Nonaka T., Suzuki K., Watanabe T.
    J. Biochem. 143:487-495(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF TRP-159.
  9. "Crystallization and preliminary X-ray analysis of plant class I chitinase from rice."
    Kezuka Y., Kitazaki K., Itoh Y., Watanabe J., Takaha O., Watanabe T., Nishizawa Y., Nonaka T.
    Protein Pept. Lett. 11:401-405(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 33-340, DISULFIDE BONDS.

Entry informationi

Entry nameiCHI2_ORYSJ
AccessioniPrimary (citable) accession number: Q7DNA1
Secondary accession number(s): Q0DID3, Q43294
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 5, 2004
Last modified: May 14, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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