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Q7DNA1

- CHI2_ORYSJ

UniProt

Q7DNA1 - CHI2_ORYSJ

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Protein

Chitinase 2

Gene

Cht2

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Hydrolyzes chitin and plays a role in defense against fungal pathogens containing chitin. Its overexpression confers enhanced resistance to sheath blight pathogen (R.solani).3 Publications

Catalytic activityi

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

GO - Molecular functioni

  1. chitinase activity Source: UniProtKB
  2. chitin binding Source: UniProtKB-KW

GO - Biological processi

  1. cell wall macromolecule catabolic process Source: InterPro
  2. chitin catabolic process Source: UniProtKB-KW
  3. defense response to fungus Source: UniProtKB
  4. polysaccharide catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Chitin degradation, Plant defense, Polysaccharide degradation

Keywords - Ligandi

Chitin-binding

Protein family/group databases

CAZyiCBM18. Carbohydrate-Binding Module Family 18.
GH19. Glycoside Hydrolase Family 19.

Names & Taxonomyi

Protein namesi
Recommended name:
Chitinase 2 (EC:3.2.1.14)
Alternative name(s):
Class I chitinase b
Short name:
OsChia1b
Pathogenesis related (PR)-3 chitinase 2
Gene namesi
Name:Cht2
Synonyms:RC7
Ordered Locus Names:Os05g0399300, LOC_Os05g33130
ORF Names:OsJ_18467
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 5

Organism-specific databases

GrameneiQ7DNA1.

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi159 – 1591W → A: Increased activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Sequence AnalysisAdd
BLAST
Chaini33 – 340308Chitinase 2PRO_5000139669Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi35 ↔ 501 PublicationPROSITE-ProRule annotation
Disulfide bondi44 ↔ 561 PublicationPROSITE-ProRule annotation
Disulfide bondi47 ↔ 741 PublicationPROSITE-ProRule annotation
Disulfide bondi49 ↔ 631 PublicationPROSITE-ProRule annotation
Disulfide bondi67 ↔ 711 PublicationPROSITE-ProRule annotation
Disulfide bondi110 ↔ 1721 PublicationPROSITE-ProRule annotation
Disulfide bondi184 ↔ 1921 PublicationPROSITE-ProRule annotation
Disulfide bondi291 ↔ 3231 PublicationPROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed in roots, sheaths and meristems.1 Publication

Gene expression databases

ExpressionAtlasiQ7DNA1. baseline.

Structurei

Secondary structure

1
340
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni37 – 415Combined sources
Helixi45 – 473Combined sources
Beta strandi54 – 596Combined sources
Helixi60 – 634Combined sources
Helixi89 – 913Combined sources
Helixi95 – 1017Combined sources
Turni102 – 1065Combined sources
Turni111 – 1144Combined sources
Helixi118 – 1269Combined sources
Turni129 – 1324Combined sources
Beta strandi134 – 1363Combined sources
Helixi137 – 15519Combined sources
Helixi166 – 1683Combined sources
Beta strandi188 – 1903Combined sources
Turni202 – 2054Combined sources
Helixi209 – 21911Combined sources
Turni223 – 2253Combined sources
Helixi229 – 2324Combined sources
Helixi234 – 24613Combined sources
Helixi255 – 2595Combined sources
Helixi267 – 2715Combined sources
Helixi278 – 29013Combined sources
Beta strandi291 – 2944Combined sources
Helixi297 – 31216Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DKVX-ray2.00A33-340[»]
3IWRX-ray2.57A/B33-340[»]
ProteinModelPortaliQ7DNA1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7DNA1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini33 – 7341Chitin-binding type-1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 chitin-binding type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

HOGENOMiHOG000231411.
InParanoidiQ7DNA1.
KOiK01183.
OMAiNCLCCSR.

Family and domain databases

Gene3Di3.30.60.10. 1 hit.
InterProiIPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamiPF00187. Chitin_bind_1. 1 hit.
PF00182. Glyco_hydro_19. 1 hit.
[Graphical view]
PIRSFiPIRSF001060. Endochitinase. 1 hit.
PRINTSiPR00451. CHITINBINDNG.
ProDomiPD000609. Chitin_bd_1. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00270. ChtBD1. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEiPS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7DNA1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSTPRAAASL AKKAALVALA VLAAALATAA RAEQCGAQAG GARCPNCLCC
60 70 80 90 100
SRWGWCGTTS DFCGDGCQSQ CSGCGPTPTP TPPSPSDGVG SIVPRDLFER
110 120 130 140 150
LLLHRNDGAC PARGFYTYEA FLAAAAAFPA FGGTGNTETR KREVAAFLGQ
160 170 180 190 200
TSHETTGGWP TAPDGPFSWG YCFKQEQNPP SDYCQPSPEW PCAPGRKYYG
210 220 230 240 250
RGPIQLSFNF NYGPAGRAIG VDLLSNPDLV ATDATVSFKT ALWFWMTPQG
260 270 280 290 300
NKPSSHDVIT GRWAPSPADA AAGRAPGYGV ITNIVNGGLE CGHGPDDRVA
310 320 330 340
NRIGFYQRYC GAFGIGTGGN LDCYNQRPFN SGSSVGLAEQ
Length:340
Mass (Da):35,587
Last modified:July 5, 2004 - v1
Checksum:i642F13E3928CA7BE
GO

Sequence cautioni

The sequence BAF17390.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16222 Genomic DNA. Translation: BAA03750.1.
X56787 mRNA. Translation: CAA40107.1.
AP008211 Genomic DNA. Translation: BAF17390.1. Sequence problems.
CM000142 Genomic DNA. Translation: EEE63650.1.
PIRiS39979.
S40414.
RefSeqiNP_001055476.1. NM_001062011.1.
UniGeneiOs.3374.

Genome annotation databases

GeneIDi4338718.
KEGGiosa:4338718.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16222 Genomic DNA. Translation: BAA03750.1 .
X56787 mRNA. Translation: CAA40107.1 .
AP008211 Genomic DNA. Translation: BAF17390.1 . Sequence problems.
CM000142 Genomic DNA. Translation: EEE63650.1 .
PIRi S39979.
S40414.
RefSeqi NP_001055476.1. NM_001062011.1.
UniGenei Os.3374.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2DKV X-ray 2.00 A 33-340 [» ]
3IWR X-ray 2.57 A/B 33-340 [» ]
ProteinModelPortali Q7DNA1.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM18. Carbohydrate-Binding Module Family 18.
GH19. Glycoside Hydrolase Family 19.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 4338718.
KEGGi osa:4338718.

Organism-specific databases

Gramenei Q7DNA1.

Phylogenomic databases

HOGENOMi HOG000231411.
InParanoidi Q7DNA1.
KOi K01183.
OMAi NCLCCSR.

Miscellaneous databases

EvolutionaryTracei Q7DNA1.

Gene expression databases

ExpressionAtlasi Q7DNA1. baseline.

Family and domain databases

Gene3Di 3.30.60.10. 1 hit.
InterProi IPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view ]
Pfami PF00187. Chitin_bind_1. 1 hit.
PF00182. Glyco_hydro_19. 1 hit.
[Graphical view ]
PIRSFi PIRSF001060. Endochitinase. 1 hit.
PRINTSi PR00451. CHITINBINDNG.
ProDomi PD000609. Chitin_bd_1. 1 hit.
[Graphical view ] [Entries sharing at least one domain ]
SMARTi SM00270. ChtBD1. 1 hit.
[Graphical view ]
SUPFAMi SSF53955. SSF53955. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEi PS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence variation, differential expression and chromosomal location of rice chitinase genes."
    Nishizawa Y., Kishimoto N., Saito A., Hibi T.
    Mol. Gen. Genet. 241:1-10(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: cv. Nipponbare.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  4. "The genomes of Oryza sativa: a history of duplications."
    Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.
    , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
    PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  5. "Enhanced resistance to sheath blight by constitutive expression of infection-related rice chitinase in transgenic elite indica rice cultivars."
    Datta K., Tu J., Oliva N., Ona I., Velazhahan R., Mew T.W., Muthukrishnan S., Datta S.K.
    Plant Sci. 160:405-414(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Distribution, structure, organ-specific expression, and phylogenic analysis of the pathogenesis-related protein-3 chitinase gene family in rice (Oryza sativa L.)."
    Nakazaki T., Tsukiyama T., Okumoto Y., Kageyama D., Naito K., Inouye K., Tanisaka T.
    Genome 49:619-630(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE, TISSUE SPECIFICITY.
  7. "Purification and characterization of a rice class I chitinase, OsChia1b, produced in Esherichia coli."
    Mizuno R., Itoh Y., Nishizawa Y., Kezuka Y., Suzuki K., Nonaka T., Watanabe T.
    Biosci. Biotechnol. Biochem. 72:893-895(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Role of the loop structure of the catalytic domain in rice class I chitinase."
    Mizuno R., Fukamizo T., Sugiyama S., Nishizawa Y., Kezuka Y., Nonaka T., Suzuki K., Watanabe T.
    J. Biochem. 143:487-495(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF TRP-159.
  9. "Crystallization and preliminary X-ray analysis of plant class I chitinase from rice."
    Kezuka Y., Kitazaki K., Itoh Y., Watanabe J., Takaha O., Watanabe T., Nishizawa Y., Nonaka T.
    Protein Pept. Lett. 11:401-405(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 33-340, DISULFIDE BONDS.

Entry informationi

Entry nameiCHI2_ORYSJ
AccessioniPrimary (citable) accession number: Q7DNA1
Secondary accession number(s): Q0DID3, Q43294
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 5, 2004
Last modified: November 26, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3