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Protein

Chitinase 2

Gene

Cht2

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes chitin and plays a role in defense against fungal pathogens containing chitin. Its overexpression confers enhanced resistance to sheath blight pathogen (R.solani).3 Publications

Catalytic activityi

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

GO - Molecular functioni

  • chitinase activity Source: UniProtKB
  • chitin binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Chitin degradation, Plant defense, Polysaccharide degradation

Keywords - Ligandi

Chitin-binding

Protein family/group databases

CAZyiCBM18. Carbohydrate-Binding Module Family 18.
GH19. Glycoside Hydrolase Family 19.

Names & Taxonomyi

Protein namesi
Recommended name:
Chitinase 2 (EC:3.2.1.14)
Alternative name(s):
Class I chitinase b
Short name:
OsChia1b
Pathogenesis related (PR)-3 chitinase 2
Gene namesi
Name:Cht2
Synonyms:RC7
Ordered Locus Names:Os05g0399300, LOC_Os05g33130
ORF Names:OsJ_18467
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 5

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi159W → A: Increased activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 32Sequence analysisAdd BLAST32
ChainiPRO_500013966933 – 340Chitinase 2Add BLAST308

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi35 ↔ 50PROSITE-ProRule annotation1 Publication
Disulfide bondi44 ↔ 56PROSITE-ProRule annotation1 Publication
Disulfide bondi47 ↔ 74PROSITE-ProRule annotation1 Publication
Disulfide bondi49 ↔ 63PROSITE-ProRule annotation1 Publication
Disulfide bondi67 ↔ 71PROSITE-ProRule annotation1 Publication
Disulfide bondi110 ↔ 172PROSITE-ProRule annotation1 Publication
Disulfide bondi184 ↔ 192PROSITE-ProRule annotation1 Publication
Disulfide bondi291 ↔ 323PROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ7DNA1.

Expressioni

Tissue specificityi

Expressed in roots, sheaths and meristems.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os05g33130.1.

Structurei

Secondary structure

1340
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni37 – 41Combined sources5
Helixi45 – 47Combined sources3
Beta strandi54 – 59Combined sources6
Helixi60 – 63Combined sources4
Helixi89 – 91Combined sources3
Helixi95 – 101Combined sources7
Turni102 – 106Combined sources5
Turni111 – 114Combined sources4
Helixi118 – 126Combined sources9
Turni129 – 132Combined sources4
Beta strandi134 – 136Combined sources3
Helixi137 – 155Combined sources19
Helixi166 – 168Combined sources3
Beta strandi188 – 190Combined sources3
Turni202 – 205Combined sources4
Helixi209 – 219Combined sources11
Turni223 – 225Combined sources3
Helixi229 – 232Combined sources4
Helixi234 – 246Combined sources13
Helixi255 – 259Combined sources5
Helixi267 – 271Combined sources5
Helixi278 – 290Combined sources13
Beta strandi291 – 294Combined sources4
Helixi297 – 312Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DKVX-ray2.00A33-340[»]
3IWRX-ray2.57A/B33-340[»]
ProteinModelPortaliQ7DNA1.
SMRiQ7DNA1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7DNA1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 73Chitin-binding type-1PROSITE-ProRule annotationAdd BLAST41

Sequence similaritiesi

Contains 1 chitin-binding type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4742. Eukaryota.
COG3979. LUCA.
HOGENOMiHOG000231411.
InParanoidiQ7DNA1.
KOiK20547.
OrthoDBiEOG09360IMR.

Family and domain databases

CDDicd00325. chitinase_glyco_hydro_19. 1 hit.
Gene3Di3.30.60.10. 1 hit.
InterProiIPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamiPF00187. Chitin_bind_1. 1 hit.
PF00182. Glyco_hydro_19. 1 hit.
[Graphical view]
PIRSFiPIRSF001060. Endochitinase. 1 hit.
PRINTSiPR00451. CHITINBINDNG.
ProDomiPD000609. Chitin_bd_1. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00270. ChtBD1. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEiPS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7DNA1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTPRAAASL AKKAALVALA VLAAALATAA RAEQCGAQAG GARCPNCLCC
60 70 80 90 100
SRWGWCGTTS DFCGDGCQSQ CSGCGPTPTP TPPSPSDGVG SIVPRDLFER
110 120 130 140 150
LLLHRNDGAC PARGFYTYEA FLAAAAAFPA FGGTGNTETR KREVAAFLGQ
160 170 180 190 200
TSHETTGGWP TAPDGPFSWG YCFKQEQNPP SDYCQPSPEW PCAPGRKYYG
210 220 230 240 250
RGPIQLSFNF NYGPAGRAIG VDLLSNPDLV ATDATVSFKT ALWFWMTPQG
260 270 280 290 300
NKPSSHDVIT GRWAPSPADA AAGRAPGYGV ITNIVNGGLE CGHGPDDRVA
310 320 330 340
NRIGFYQRYC GAFGIGTGGN LDCYNQRPFN SGSSVGLAEQ
Length:340
Mass (Da):35,587
Last modified:July 5, 2004 - v1
Checksum:i642F13E3928CA7BE
GO

Sequence cautioni

The sequence BAF17390 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16222 Genomic DNA. Translation: BAA03750.1.
X56787 mRNA. Translation: CAA40107.1.
AP008211 Genomic DNA. Translation: BAF17390.1. Sequence problems.
AP014961 Genomic DNA. No translation available.
CM000142 Genomic DNA. Translation: EEE63650.1.
PIRiS39979.
S40414.
RefSeqiXP_015640432.1. XM_015784946.1.
UniGeneiOs.3374.

Genome annotation databases

GeneIDi4338718.
KEGGiosa:4338718.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16222 Genomic DNA. Translation: BAA03750.1.
X56787 mRNA. Translation: CAA40107.1.
AP008211 Genomic DNA. Translation: BAF17390.1. Sequence problems.
AP014961 Genomic DNA. No translation available.
CM000142 Genomic DNA. Translation: EEE63650.1.
PIRiS39979.
S40414.
RefSeqiXP_015640432.1. XM_015784946.1.
UniGeneiOs.3374.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DKVX-ray2.00A33-340[»]
3IWRX-ray2.57A/B33-340[»]
ProteinModelPortaliQ7DNA1.
SMRiQ7DNA1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os05g33130.1.

Protein family/group databases

CAZyiCBM18. Carbohydrate-Binding Module Family 18.
GH19. Glycoside Hydrolase Family 19.

Proteomic databases

PaxDbiQ7DNA1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi4338718.
KEGGiosa:4338718.

Phylogenomic databases

eggNOGiKOG4742. Eukaryota.
COG3979. LUCA.
HOGENOMiHOG000231411.
InParanoidiQ7DNA1.
KOiK20547.
OrthoDBiEOG09360IMR.

Miscellaneous databases

EvolutionaryTraceiQ7DNA1.

Family and domain databases

CDDicd00325. chitinase_glyco_hydro_19. 1 hit.
Gene3Di3.30.60.10. 1 hit.
InterProiIPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamiPF00187. Chitin_bind_1. 1 hit.
PF00182. Glyco_hydro_19. 1 hit.
[Graphical view]
PIRSFiPIRSF001060. Endochitinase. 1 hit.
PRINTSiPR00451. CHITINBINDNG.
ProDomiPD000609. Chitin_bd_1. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00270. ChtBD1. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEiPS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCHI2_ORYSJ
AccessioniPrimary (citable) accession number: Q7DNA1
Secondary accession number(s): Q0DID3, Q43294
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.