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Reviewed, UniProtKB/Swiss-Prot Q7DNA1 (CHI2_ORYSJ)

Last modified November 24, 2009. Version 39. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Chitinase 2
    EC=3.2.1.14
Alternative name(s):
    Pathogenesis related (PR)-3 chitinase 2
    Class I chitinase b
      Short name=OsChia1b
Gene names
Name: Cht2
Synonyms: RC7
Ordered Locus Names: Os05g0399300, LOC_Os05g33130
ORF Names: OsJ_18467
OrganismOryza sativa subsp. japonica (Rice)
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

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Protein attributes

Sequence length340 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Hydrolyzes chitin and plays a role in defense against fungal pathogens containing chitin. Its overexpression confers enhanced resistance to sheath blight pathogen (R.solani). Ref.5 Ref.7 Ref.8

Catalytic activity

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

Tissue specificity

Expressed in roots, sheaths and meristems. Ref.6

Sequence similarities

Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily.

Contains 1 chitin-binding type-1 domain.

Sequence caution

The sequence BAF17390.1 differs from that shown. Reason: Erroneous gene model prediction.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3232 Potential
Chain33 – 340308Chitinase 2
PRO_5000139669

Regions

Domain33 – 7341Chitin-binding type-1

Amino acid modifications

Disulfide bond35 ↔ 50 Ref.9
Disulfide bond44 ↔ 56 Ref.9
Disulfide bond47 ↔ 74 Ref.9
Disulfide bond49 ↔ 63 Ref.9
Disulfide bond67 ↔ 71 Ref.9
Disulfide bond110 ↔ 172 Ref.9
Disulfide bond184 ↔ 192 Ref.9
Disulfide bond291 ↔ 323 Ref.9

Experimental info

Mutagenesis1591W → A: Increased activity. Ref.8

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Sequences

Sequence LengthMass (Da)Tools
Q7DNA1-1 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 642F13E3928CA7BE

FASTA34035,587
        10         20         30         40         50         60 
MSTPRAAASL AKKAALVALA VLAAALATAA RAEQCGAQAG GARCPNCLCC SRWGWCGTTS 

        70         80         90        100        110        120 
DFCGDGCQSQ CSGCGPTPTP TPPSPSDGVG SIVPRDLFER LLLHRNDGAC PARGFYTYEA 

       130        140        150        160        170        180 
FLAAAAAFPA FGGTGNTETR KREVAAFLGQ TSHETTGGWP TAPDGPFSWG YCFKQEQNPP 

       190        200        210        220        230        240 
SDYCQPSPEW PCAPGRKYYG RGPIQLSFNF NYGPAGRAIG VDLLSNPDLV ATDATVSFKT 

       250        260        270        280        290        300 
ALWFWMTPQG NKPSSHDVIT GRWAPSPADA AAGRAPGYGV ITNIVNGGLE CGHGPDDRVA 

       310        320        330        340 
NRIGFYQRYC GAFGIGTGGN LDCYNQRPFN SGSSVGLAEQ

« Hide

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References

« Hide 'large scale' references
[1]"Sequence variation, differential expression and chromosomal location of rice chitinase genes."
Nishizawa Y., Kishimoto N., Saito A., Hibi T.
Mol. Gen. Genet. 241:1-10(1993) [PubMed: 7901749] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Strain: cv. Nipponbare.
[2]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed: 16100779] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana."
The rice annotation project (RAP)
Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: cv. Nipponbare.
[4]"The genomes of Oryza sativa: a history of duplications."
Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. expand/collapse author list , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
PLoS Biol. 3:266-281(2005) [PubMed: 15685292] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[5]"Enhanced resistance to sheath blight by constitutive expression of infection-related rice chitinase in transgenic elite indica rice cultivars."
Datta K., Tu J., Oliva N., Ona I., Velazhahan R., Mew T.W., Muthukrishnan S., Datta S.K.
Plant Sci. 160:405-414(2001) [PubMed: 11166426] [Abstract]
Cited for: FUNCTION.
[6]"Distribution, structure, organ-specific expression, and phylogenic analysis of the pathogenesis-related protein-3 chitinase gene family in rice (Oryza sativa L.)."
Nakazaki T., Tsukiyama T., Okumoto Y., Kageyama D., Naito K., Inouye K., Tanisaka T.
Genome 49:619-630(2006) [PubMed: 16936841] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE, TISSUE SPECIFICITY.
[7]"Purification and characterization of a rice class I chitinase, OsChia1b, produced in Esherichia coli."
Mizuno R., Itoh Y., Nishizawa Y., Kezuka Y., Suzuki K., Nonaka T., Watanabe T.
Biosci. Biotechnol. Biochem. 72:893-895(2008) [PubMed: 18323646] [Abstract]
Cited for: FUNCTION.
[8]"Role of the loop structure of the catalytic domain in rice class I chitinase."
Mizuno R., Fukamizo T., Sugiyama S., Nishizawa Y., Kezuka Y., Nonaka T., Suzuki K., Watanabe T.
J. Biochem. 143:487-495(2008) [PubMed: 18211919] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF TRP-159.
[9]"Crystallization and preliminary X-ray analysis of plant class I chitinase from rice."
Kezuka Y., Kitazaki K., Itoh Y., Watanabe J., Takaha O., Watanabe T., Nishizawa Y., Nonaka T.
Protein Pept. Lett. 11:401-405(2004) [PubMed: 15327374] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 33-340, DISULFIDE BONDS.

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Cross-references

Sequence databases

D16222 Genomic DNA. Translation: BAA03750.1.
X56787 mRNA. Translation: CAA40107.1.
AP008211 Genomic DNA. Translation: BAF17390.1. Sequence problems.
CM000142 Genomic DNA. Translation: EEE63650.1.
PIRS39979.
S40414.
RefSeqNP_001055476.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2DKVX-ray2.00A33-340[»]
ModBaseSearch...

Protein family/group databases

CAZyCBM18. Carbohydrate-Binding Module Family 18.
GH19. Glycoside Hydrolase Family 19.

Genome annotation databases

GeneID4338718.
GenomeReviewsGene locus Cht2 in contig AP008211_GR.

Organism-specific databases

GrameneQ7DNA1.

Family and domain databases

InterProIPR018371. Chitin-binding_1_CS.
IPR001002. Chitin_bd_1.
IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
[Graphical view]
Gene3DG3DSA:3.30.60.10. Chitin_bd_1. 1 hit.
PANTHERPTHR22595. Glyco_hydro_19_cat. 1 hit.
PfamPF00187. Chitin_bind_1. 1 hit.
PF00182. Glyco_hydro_19. 1 hit.
[Graphical view]
PIRSFPIRSF001060. Endochitinase. 1 hit.
PRINTSPR00451. CHITINBINDNG.
ProDomPD000609. Chitin_bd_1. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00270. ChtBD1. 1 hit.
[Graphical view]
PROSITEPS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameCHI2_ORYSJ
AccessionPrimary (citable) accession number: Q7DNA1
Secondary accession number(s): Q0DID3, Q43294
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 5, 2004
Last modified: November 24, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents