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Protein

ABC transporter C family member 4

Gene

ABCC4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the regulation of stomatal aperture. May function as a high-capacity pump for folates.1 Publication

Catalytic activityi

ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).

Enzyme regulationi

Inhibited by methotrexate.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi676 – 683ATP 1PROSITE-ProRule annotation8
Nucleotide bindingi1308 – 1315ATP 2PROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

  • drug transmembrane transport Source: TAIR
  • response to nematode Source: TAIR
  • response to water deprivation Source: TAIR
  • response to wounding Source: TAIR
  • stomatal movement Source: TAIR
  • transmembrane transport Source: GO_Central

Keywordsi

Molecular functionHydrolase
Biological processTransport
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G47800-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ABC transporter C family member 4 (EC:3.6.3.44)
Short name:
ABC transporter ABCC.4
Short name:
AtABCC4
Alternative name(s):
ATP-energized glutathione S-conjugate pump 4
Glutathione S-conjugate-transporting ATPase 4
Multidrug resistance-associated protein 4
Gene namesi
Name:ABCC4
Synonyms:EST3, MRP4
Ordered Locus Names:At2g47800
ORF Names:F17A22.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G47800.
TAIRilocus:2043268. AT2G47800.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei46 – 66HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei103 – 123HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei133 – 153HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei170 – 190HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei203 – 223HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei326 – 346HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei361 – 381HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei439 – 459HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei463 – 483HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei551 – 571HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei953 – 973HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei992 – 1012HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei1067 – 1087HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei1089 – 1109HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei1186 – 1206HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei1211 – 1231HelicalPROSITE-ProRule annotationAdd BLAST21

Keywords - Cellular componenti

Cell membrane, Membrane, Vacuole

Pathology & Biotechi

Disruption phenotypei

Plants have larger stomatal aperture and increased drought susceptibility, but are not affected by the ABA signal transduction pathway.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002260751 – 1516ABC transporter C family member 4Add BLAST1516

Proteomic databases

PaxDbiQ7DM58.
PRIDEiQ7DM58.

PTM databases

iPTMnetiQ7DM58.

Expressioni

Tissue specificityi

Ubiquitous. High expression in the guard cells.2 Publications

Inductioni

By salicylic acid (SA), menadione and, to a lower extent, by 1-chloro-2,4-dinitrobenzene (CDNB), benoxacor, cloquintocet, fenchlorazol and fluorazol.3 Publications

Gene expression databases

GenevisibleiQ7DM58. AT.

Interactioni

Protein-protein interaction databases

BioGridi4727. 1 interactor.
STRINGi3702.AT2G47800.1.

Structurei

3D structure databases

ProteinModelPortaliQ7DM58.
SMRiQ7DM58.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini326 – 607ABC transmembrane type-1 1PROSITE-ProRule annotationAdd BLAST282
Domaini641 – 864ABC transporter 1PROSITE-ProRule annotationAdd BLAST224
Domaini957 – 1237ABC transmembrane type-1 2PROSITE-ProRule annotationAdd BLAST281
Domaini1274 – 1508ABC transporter 2PROSITE-ProRule annotationAdd BLAST235

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0054. Eukaryota.
COG1132. LUCA.
InParanoidiQ7DM58.
OMAiKVSCDRI.
OrthoDBiEOG0936007A.
PhylomeDBiQ7DM58.

Family and domain databases

Gene3Di1.20.1560.10. 2 hits.
InterProiView protein in InterPro
IPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR036640. ABC1_TM_sf.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
PfamiView protein in Pfam
PF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
SMARTiView protein in SMART
SM00382. AAA. 2 hits.
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEiView protein in PROSITE
PS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 2 hits.

Sequencei

Sequence statusi: Complete.

Q7DM58-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWLLSSSPWL SELSCSYSAV VEHTSSVPVP IQWLRFVLLS PCPQRALFSA
60 70 80 90 100
VDFIFLLCFA LHKLFSSPSS SSEINGHAEI RKPLIGIRGR TPTRTTAWFK
110 120 130 140 150
TTVAVTVLLS FCSVVLCVLA FTGKRRTQRP WNLIDPLFWL IHAVTHLVIA
160 170 180 190 200
VLVLHQKRFA ALNHPLSLRI YWISSFVLTS LFAVTGIFHF LSDAATSLRA
210 220 230 240 250
EDVASFFSFP LTAFLLIASV RGITGLVTAE TNSPTKPSDA VSVEKSDNVS
260 270 280 290 300
LYASASVFSK TFWLWMNPLL SKGYKSPLTL EQVPTLSPEH KAERLALLFE
310 320 330 340 350
SSWPKPSENS SHPIRTTLLR CFWKEILFTA ILAIVRLGVM YVGPVLIQSF
360 370 380 390 400
VDFTSGKRSS PWQGYYLVLI LLVAKFVEVL TTHQFNFDSQ KLGMLIRSTL
410 420 430 440 450
ITALYKKGLK LTGSARQNHG VGQIVNYMAV DAQQLSDMML QLHAIWLMPL
460 470 480 490 500
QVTVALVLLY GSLGASVITA VIGLTGVFVF ILLGTQRNNG YQFSLMGNRD
510 520 530 540 550
SRMKATNEML NYMRVIKFQA WENHFNKRIL KFRDMEFGWL SKFLYSIAGN
560 570 580 590 600
IIVLWSTPVL ISALTFATAL ALGVKLDAGT VFTTTTIFKI LQEPIRTFPQ
610 620 630 640 650
SMISLSQAMI SLGRLDSYMM SKELSEDAVE RALGCDGNTA VEVRDGSFSW
660 670 680 690 700
DDEDNEPALS DINFKVKKGE LTAIVGTVGS GKSSLLASVL GEMHRISGQV
710 720 730 740 750
RVCGSTGYVA QTSWIENGTV QDNILFGLPM VREKYNKVLN VCSLEKDLQM
760 770 780 790 800
MEFGDKTEIG ERGINLSGGQ KQRIQLARAV YQECDVYLLD DVFSAVDAHT
810 820 830 840 850
GSDIFKKCVR GALKGKTVLL VTHQVDFLHN VDCILVMRDG KIVESGKYDE
860 870 880 890 900
LVSSGLDFGE LVAAHETSME LVEAGADSAA VATSPRTPTS PHASSPRTSM
910 920 930 940 950
ESPHLSDLND EHIKSFLGSH IVEDGSKLIK EEERETGQVS LGVYKQYCTE
960 970 980 990 1000
AYGWWGIVLV LFFSLTWQGS LMASDYWLAY ETSAKNAISF DASVFILGYV
1010 1020 1030 1040 1050
IIALVSIVLV SIRSYYVTHL GLKTAQIFFR QILNSILHAP MSFFDTTPSG
1060 1070 1080 1090 1100
RILSRASTDQ TNVDILIPFM LGLVVSMYTT LLSIFIVTCQ YAWPTAFFVI
1110 1120 1130 1140 1150
PLGWLNIWYR NYYLASSREL TRMDSITKAP IIHHFSESIA GVMTIRSFRK
1160 1170 1180 1190 1200
QELFRQENVK RVNDNLRMDF HNNGSNEWLG FRLELVGSWV LCISALFMVL
1210 1220 1230 1240 1250
LPSNVIRPEN VGLSLSYGLS LNSVLFFAIY MSCFVENKMV SVERIKQFTD
1260 1270 1280 1290 1300
IPSESEWERK ETLPPSNWPF HGNVHLEDLK VRYRPNTPLV LKGITLDIKG
1310 1320 1330 1340 1350
GEKVGVVGRT GSGKSTLIQV LFRLVEPSGG KIIIDGIDIS TLGLHDLRSR
1360 1370 1380 1390 1400
FGIIPQEPVL FEGTVRSNID PTEQYSDEEI WKSLERCQLK DVVATKPEKL
1410 1420 1430 1440 1450
DSLVVDNGEN WSVGQRQLLC LGRVMLKRSR LLFLDEATAS VDSQTDAVIQ
1460 1470 1480 1490 1500
KIIREDFASC TIISIAHRIP TVMDGDRVLV IDAGKAKEFD SPARLLERPS
1510
LFAALVQEYA LRSAGI
Length:1,516
Mass (Da):169,080
Last modified:March 7, 2006 - v2
Checksum:i047751628C58431E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1234F → L in BAE98764 (Ref. 5) Curated1
Sequence conflicti1295T → I in BAE98764 (Ref. 5) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ002584 Genomic DNA. Translation: CAA05625.1.
AF243509 mRNA. Translation: AAF68441.1.
AC005309 Genomic DNA. Translation: AAC63634.1.
CP002685 Genomic DNA. Translation: AEC10889.1.
AK226656 mRNA. Translation: BAE98764.1.
U96399 mRNA. Translation: AAC49797.1.
PIRiF84919.
RefSeqiNP_182301.1. NM_130347.5.
UniGeneiAt.37.

Genome annotation databases

EnsemblPlantsiAT2G47800.1; AT2G47800.1; AT2G47800.
GeneIDi819392.
GrameneiAT2G47800.1; AT2G47800.1; AT2G47800.
KEGGiath:AT2G47800.

Similar proteinsi

Entry informationi

Entry nameiAB4C_ARATH
AccessioniPrimary (citable) accession number: Q7DM58
Secondary accession number(s): O24525, Q0WVT4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: March 7, 2006
Last modified: October 25, 2017
This is version 109 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families