Reviewed, UniProtKB/Swiss-Prot Q7DM58 (AB4C_ARATH)

Names and origin

Protein names

Recommended name:
ABC transporter C family member 4
Short name=ABC transporter ABCC.4
Short name=AtABCC4
EC=3.6.3.44

Alternative name(s):
Multidrug resistance-associated protein 4
Glutathione S-conjugate-transporting ATPase 4
ATP-energized glutathione S-conjugate pump 4

Gene names

Name:	ABCC4
Synonyms:	EST3, MRP4
Ordered Locus Names:	At2g47800
ORF Names:	F17A22.19

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	1516 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the regulation of stomatal aperture. May function as a high-capacity pump for folates. Ref.9
Catalytic activity	ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).
Enzyme regulation	Inhibited by methotrexate. Ref.9
Subcellular location	Cell membrane; Multi-pass membrane protein. Vacuole membrane; Multi-pass membrane protein. Note: Cell membrane in Ref.9, Vacuole membrane in Ref.10. Ref.9 Ref.10
Tissue specificity	Ubiquitous. High expression in the guard cells. Ref.9 Ref.8
Induction	By salicylic acid (SA), menadione and, to a lower extent, by 1-chloro-2,4-dinitrobenzene (CDNB), benoxacor, cloquintocet, fenchlorazol and fluorazol. Ref.9 Ref.8 Ref.1 Ref.5
Disruption phenotype	Plants have larger stomatal aperture and increased drought susceptibility, but are not affected by the ABA signal transduction pathway. Ref.9
Sequence similarities	Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily. [View classification] Contains 2 ABC transmembrane type-1 domains. Contains 2 ABC transporter domains.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Membrane Vacuole
Domain	Repeat Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	response to nematode Inferred from expression pattern. Source: TAIR response to water deprivation Ref.9 Inferred from mutant phenotype. Source: TAIR response to wounding Inferred from expression pattern. Source: TAIR stomatal movement Ref.9 Inferred from mutant phenotype. Source: TAIR transmembrane transport Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plant-type vacuole Ref.10 Inferred from direct assay. Source: TAIR plasma membrane Ref.9 Inferred from direct assay. Source: TAIR vacuolar membrane Ref.10 Inferred from direct assay. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW folic acid transporter activity Ref.9 Inferred from genetic interaction. Source: TAIR xenobiotic-transporting ATPase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1516	1516	ABC transporter C family member 4		PRO_0000226075
Regions
	Transmembrane	46 – 66	21	Potential
	Transmembrane	103 – 123	21	Potential
	Transmembrane	133 – 153	21	Potential
	Transmembrane	170 – 190	21	Potential
	Transmembrane	203 – 223	21	Potential
	Transmembrane	326 – 346	21	Potential
	Transmembrane	361 – 381	21	Potential
	Transmembrane	439 – 459	21	Potential
	Transmembrane	463 – 483	21	Potential
	Transmembrane	551 – 571	21	Potential
	Transmembrane	953 – 973	21	Potential
	Transmembrane	992 – 1012	21	Potential
	Transmembrane	1067 – 1087	21	Potential
	Transmembrane	1089 – 1109	21	Potential
	Transmembrane	1186 – 1206	21	Potential
	Transmembrane	1211 – 1231	21	Potential
	Domain	326 – 607	282	ABC transmembrane type-1 1
	Domain	641 – 864	224	ABC transporter 1
	Domain	957 – 1237	281	ABC transmembrane type-1 2
	Domain	1274 – 1508	235	ABC transporter 2
	Nucleotide binding	676 – 683	8	ATP 1 Potential
	Nucleotide binding	1308 – 1315	8	ATP 2 Potential
Experimental info
	Sequence conflict	1234	1	F → L in BAE98764. Ref.4
	Sequence conflict	1295	1	T → I in BAE98764. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q7DM58-1 [UniParc]. Last modified March 7, 2006. Version 2. Checksum: 047751628C58431E	FASTA	1,516	169,080
	10 20 30 40 50 60 MWLLSSSPWL SELSCSYSAV VEHTSSVPVP IQWLRFVLLS PCPQRALFSA VDFIFLLCFA 70 80 90 100 110 120 LHKLFSSPSS SSEINGHAEI RKPLIGIRGR TPTRTTAWFK TTVAVTVLLS FCSVVLCVLA 130 140 150 160 170 180 FTGKRRTQRP WNLIDPLFWL IHAVTHLVIA VLVLHQKRFA ALNHPLSLRI YWISSFVLTS 190 200 210 220 230 240 LFAVTGIFHF LSDAATSLRA EDVASFFSFP LTAFLLIASV RGITGLVTAE TNSPTKPSDA 250 260 270 280 290 300 VSVEKSDNVS LYASASVFSK TFWLWMNPLL SKGYKSPLTL EQVPTLSPEH KAERLALLFE 310 320 330 340 350 360 SSWPKPSENS SHPIRTTLLR CFWKEILFTA ILAIVRLGVM YVGPVLIQSF VDFTSGKRSS 370 380 390 400 410 420 PWQGYYLVLI LLVAKFVEVL TTHQFNFDSQ KLGMLIRSTL ITALYKKGLK LTGSARQNHG 430 440 450 460 470 480 VGQIVNYMAV DAQQLSDMML QLHAIWLMPL QVTVALVLLY GSLGASVITA VIGLTGVFVF 490 500 510 520 530 540 ILLGTQRNNG YQFSLMGNRD SRMKATNEML NYMRVIKFQA WENHFNKRIL KFRDMEFGWL 550 560 570 580 590 600 SKFLYSIAGN IIVLWSTPVL ISALTFATAL ALGVKLDAGT VFTTTTIFKI LQEPIRTFPQ 610 620 630 640 650 660 SMISLSQAMI SLGRLDSYMM SKELSEDAVE RALGCDGNTA VEVRDGSFSW DDEDNEPALS 670 680 690 700 710 720 DINFKVKKGE LTAIVGTVGS GKSSLLASVL GEMHRISGQV RVCGSTGYVA QTSWIENGTV 730 740 750 760 770 780 QDNILFGLPM VREKYNKVLN VCSLEKDLQM MEFGDKTEIG ERGINLSGGQ KQRIQLARAV 790 800 810 820 830 840 YQECDVYLLD DVFSAVDAHT GSDIFKKCVR GALKGKTVLL VTHQVDFLHN VDCILVMRDG 850 860 870 880 890 900 KIVESGKYDE LVSSGLDFGE LVAAHETSME LVEAGADSAA VATSPRTPTS PHASSPRTSM 910 920 930 940 950 960 ESPHLSDLND EHIKSFLGSH IVEDGSKLIK EEERETGQVS LGVYKQYCTE AYGWWGIVLV 970 980 990 1000 1010 1020 LFFSLTWQGS LMASDYWLAY ETSAKNAISF DASVFILGYV IIALVSIVLV SIRSYYVTHL 1030 1040 1050 1060 1070 1080 GLKTAQIFFR QILNSILHAP MSFFDTTPSG RILSRASTDQ TNVDILIPFM LGLVVSMYTT 1090 1100 1110 1120 1130 1140 LLSIFIVTCQ YAWPTAFFVI PLGWLNIWYR NYYLASSREL TRMDSITKAP IIHHFSESIA 1150 1160 1170 1180 1190 1200 GVMTIRSFRK QELFRQENVK RVNDNLRMDF HNNGSNEWLG FRLELVGSWV LCISALFMVL 1210 1220 1230 1240 1250 1260 LPSNVIRPEN VGLSLSYGLS LNSVLFFAIY MSCFVENKMV SVERIKQFTD IPSESEWERK 1270 1280 1290 1300 1310 1320 ETLPPSNWPF HGNVHLEDLK VRYRPNTPLV LKGITLDIKG GEKVGVVGRT GSGKSTLIQV 1330 1340 1350 1360 1370 1380 LFRLVEPSGG KIIIDGIDIS TLGLHDLRSR FGIIPQEPVL FEGTVRSNID PTEQYSDEEI 1390 1400 1410 1420 1430 1440 WKSLERCQLK DVVATKPEKL DSLVVDNGEN WSVGQRQLLC LGRVMLKRSR LLFLDEATAS 1450 1460 1470 1480 1490 1500 VDSQTDAVIQ KIIREDFASC TIISIAHRIP TVMDGDRVLV IDAGKAKEFD SPARLLERPS 1510 LFAALVQEYA LRSAGI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression analyses of the AtMRP4, a novel MRP-like gene from Arabidopsis thaliana." Sanchez-Fernandez R., Ardiles-Diaz W., Van Montagu M., Inze D., May M.J. Mol. Gen. Genet. 258:655-662(1998) [PubMed: 9671034] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION.
[2]	"AtMRP4 gene of Arabidopsis encodes a glutathione S-conjugate pump." Sanchez-Fernandez R., Rea P.A. Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[3]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Differential expression of genes coding for ABC transporters after treatment of Arabidopsis thaliana with xenobiotics." Tommasini R., Vogt E., Schmid J., Fromentau M., Amrhein N., Martinoia E. FEBS Lett. 411:206-210(1997) [PubMed: 9271206] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1272-1516, INDUCTION.
[6]	"The Arabidopsis thaliana ABC protein superfamily, a complete inventory." Sanchez-Fernandez R., Davies T.G., Coleman J.O., Rea P.A. J. Biol. Chem. 276:30231-30244(2001) [PubMed: 11346655] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[7]	"Multifunctionality of plant ABC transporters -- more than just detoxifiers." Martinoia E., Klein M., Geisler M., Bovet L., Forestier C., Kolukisaoglu H.U., Mueller-Roeber B., Schulz B. Planta 214:345-355(2002) [PubMed: 11855639] [Abstract] Cited for: GENE FAMILY.
[8]	"Family business: the multidrug-resistance related protein (MRP) ABC transporter genes in Arabidopsis thaliana." Kolukisaoglu U.H., Bovet L., Klein M., Eggmann T., Geisler M., Wanke D., Martinoia E., Schulz B. Planta 216:107-119(2002) [PubMed: 12430019] [Abstract] Cited for: TISSUE SPECIFICITY, INDUCTION.
[9]	"Disruption of AtMRP4, a guard cell plasma membrane ABCC-type ABC transporter, leads to deregulation of stomatal opening and increased drought susceptibility." Klein M., Geisler M., Suh S.J., Kolukisaoglu U.H., Azevedo L., Plaza S., Curtis M.D., Richter A., Weder B., Schulz B., Martinoia E. Plant J. 39:219-236(2004) [PubMed: 15225287] [Abstract] Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION, ENZYME REGULATION, FUNCTION, DISRUPTION PHENOTYPE.
[10]	"Mapping the Arabidopsis organelle proteome." Dunkley T.P.J., Hester S., Shadforth I.P., Runions J., Weimar T., Hanton S.L., Griffin J.L., Bessant C., Brandizzi F., Hawes C., Watson R.B., Dupree P., Lilley K.S. Proc. Natl. Acad. Sci. U.S.A. 103:6518-6523(2006) [PubMed: 16618929] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION.
[11]	"Plant ABC proteins - a unified nomenclature and updated inventory." Verrier P.J., Bird D., Burla B., Dassa E., Forestier C., Geisler M., Klein M., Kolukisaoglu H.U., Lee Y., Martinoia E., Murphy A., Rea P.A., Samuels L., Schulz B., Spalding E.J., Yazaki K., Theodoulou F.L. Trends Plant Sci. 13:151-159(2008) [PubMed: 18299247] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ002584 Genomic DNA. Translation: CAA05625.1. AF243509 mRNA. Translation: AAF68441.1. AC005309 Genomic DNA. Translation: AAC63634.1. AK226656 mRNA. Translation: BAE98764.1. U96399 mRNA. Translation: AAC49797.1.
IPI	IPI00538325.
PIR	F84919.
RefSeq	NP_182301.1.
UniGene	At.37
3D structure databases
SMR	Q7DM58. Positions 954-1507, 1273-1508.
ModBase	Search...
Proteomic databases
PRIDE	Q7DM58.
ProMEX	Q7DM58.
Genome annotation databases
GeneID	819392.
GenomeReviews	Gene locus AT2G47800 in contig CT485783_GR.
KEGG	ath:AT2G47800.
NMPDR	fig|3702.1.peg.11966.
Organism-specific databases
TAIR	At2g47800.
Phylogenomic databases
eggNOG	KOG0054.
HOGENOM	HBG758042.
InParanoid	Q7DM58.
OMA	FRESEFG.
PhylomeDB	Q7DM58.
Enzyme and pathway databases
BRENDA	3.6.3.44. 302.
Gene expression databases
Genevestigator	Q7DM58.
GermOnline	AT2G47800. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR017940. ABC_transporter_type1. IPR001140. ABC_transptr_TM_dom. IPR011527. ABC_transptrTM_dom_typ1. IPR003593. ATPase_AAA+_core. [Graphical view]
Pfam	PF00664. ABC_membrane. 2 hits. PF00005. ABC_tran. 2 hits. [Graphical view]
SMART	SM00382. AAA. 2 hits. [Graphical view]
SUPFAM	SSF90123. ABC_TM_1. 2 hits.
PROSITE	PS50929. ABC_TM1F. 2 hits. PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AB4C_ARATH

Accession

Primary (citable) accession number: Q7DM58
Secondary accession number(s): O24525, Q0WVT4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	March 7, 2006
Last modified:	March 2, 2010
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)