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Q7DLS1 (PSB7B_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proteasome subunit beta type-7-B

EC=3.4.25.1
Alternative name(s):
20S proteasome beta subunit B-2
Proteasome component FC
Proteasome subunit beta type-2
Gene names
Name:PBB2
Synonyms:PRCFC
Ordered Locus Names:At5g40580
ORF Names:MNF13.10
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length274 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activity

Cleavage of peptide bonds with very broad specificity.

Subunit structure

Component of the 20S core complex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Ref.7 Ref.8

Subcellular location

Cytoplasm By similarity. Nucleus By similarity.

Sequence similarities

Belongs to the peptidase T1B family.

Ontologies

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q7DLS1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Propeptide1 – 3737Removed in mature form
PRO_0000042832
Chain38 – 274237Proteasome subunit beta type-7-B
PRO_0000042833

Sites

Active site381Nucleophile By similarity

Experimental info

Sequence conflict1731A → V in AAM13010. Ref.4
Sequence conflict1731A → V in AAM47910. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 22, 2005. Version 2.
Checksum: 4AD31807A97D4C51

FASTA27429,617
        10         20         30         40         50         60 
MSQSSVDIPP KGGFSFDLCK RNDMLTQKGL KAPSFLKTGT TIVGLIFKDG VILGADTRAT 

        70         80         90        100        110        120 
EGPIVADKNC EKIHYMAPNI YCCGAGTAAD TEAVTDMVSS QLRLHRYQTG RDSRVVTALT 

       130        140        150        160        170        180 
LLKKHLFSYQ GHVSAALVLG GVDITGPHLH TIYPHGSTDT LPFATMGSGS LAAMSVFEAK 

       190        200        210        220        230        240 
YKEGLTRDEG IKLVAEAICS GIFNDLGSGS NVDICVITKG HKEYLRNYME PNPRTYVSSK 

       250        260        270 
GYSFTKKTEV LLTKITPLLE RVEIVEVAGE AMEE 

« Hide

References

« Hide 'large scale' references
[1]"Molecular organization of the 20S proteasome gene family from Arabidopsis thaliana."
Fu H., Doelling J.H., Arendt C.S., Hochstrasser M., Vierstra R.D.
Genetics 149:677-692(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE.
Strain: cv. Columbia.
[2]"Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence features of the regions of 1,456,315 bp covered by nineteen physically assigned P1 and TAC clones."
Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N., Tabata S.
DNA Res. 5:41-54(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Full-length cDNA from Arabidopsis thaliana."
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]"The 20S proteasome gene family in Arabidopsis thaliana."
Parmentier Y., Bouchez D., Fleck J., Genschik P.
FEBS Lett. 416:281-285(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 57-274.
Strain: cv. Columbia.
[7]"Structure and functional analyses of the 26S proteasome subunits from plants."
Fu H., Girod P.-A., Doelling J.H., van Nocker S., Hochstrasser M., Finley D., Vierstra R.D.
Mol. Biol. Rep. 26:137-146(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT.
[8]"Affinity purification of the Arabidopsis 26 S proteasome reveals a diverse array of plant proteolytic complexes."
Book A.J., Gladman N.P., Lee S.S., Scalf M., Smith L.M., Vierstra R.D.
J. Biol. Chem. 285:25554-25569(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, CHARACTERIZATION OF THE 26S PROTEASOME COMPLEX, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF043531 mRNA. Translation: AAC32067.1.
AB009052 Genomic DNA. Translation: BAB08528.1.
CP002688 Genomic DNA. Translation: AED94567.1.
CP002688 Genomic DNA. Translation: AED94568.1.
AY093011 mRNA. Translation: AAM13010.1.
AY114591 mRNA. Translation: AAM47910.1.
AY087750 mRNA. Translation: AAM65286.1.
Y13177 mRNA. Translation: CAA73620.1.
PIRT51979.
RefSeqNP_198874.1. NM_123422.3. [Q7DLS1-1]
NP_851108.1. NM_180777.2. [Q7DLS1-1]
UniGeneAt.47287.
At.66685.

3D structure databases

ProteinModelPortalQ7DLS1.
SMRQ7DLS1. Positions 40-258.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid19307. 2 interactions.
IntActQ7DLS1. 1 interaction.

Protein family/group databases

MEROPST01.011.

Proteomic databases

PaxDbQ7DLS1.
PRIDEQ7DLS1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT5G40580.1; AT5G40580.1; AT5G40580. [Q7DLS1-1]
AT5G40580.2; AT5G40580.2; AT5G40580. [Q7DLS1-1]
GeneID834056.
KEGGath:AT5G40580.

Organism-specific databases

GeneFarm5120.
TAIRAT5G40580.

Phylogenomic databases

eggNOGCOG0638.
HOGENOMHOG000182856.
InParanoidQ7DLS1.
KOK02739.
OMAQIWCAGA.
PhylomeDBQ7DLS1.

Enzyme and pathway databases

BioCycARA:AT5G40580-MONOMER.
ARA:GQT-2095-MONOMER.
ARA:GQT-2725-MONOMER.

Gene expression databases

GenevestigatorQ7DLS1.

Family and domain databases

Gene3D3.60.20.10. 1 hit.
InterProIPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSPR00141. PROTEASOME.
SUPFAMSSF56235. SSF56235. 1 hit.
PROSITEPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePSB7B_ARATH
AccessionPrimary (citable) accession number: Q7DLS1
Secondary accession number(s): O23709, Q8LAK8, Q8RWL9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: June 11, 2014
This is version 81 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names