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Protein

Proteasome subunit beta type-7-B

Gene

PBB2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei38NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiR-ATH-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-ATH-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-ATH-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-ATH-382556. ABC-family proteins mediated transport.
R-ATH-4641258. Degradation of DVL.
R-ATH-5632684. Hedgehog 'on' state.
R-ATH-5668541. TNFR2 non-canonical NF-kB pathway.
R-ATH-5689603. UCH proteinases.
R-ATH-5689880. Ub-specific processing proteases.
R-ATH-6798695. Neutrophil degranulation.
R-ATH-68949. Orc1 removal from chromatin.
R-ATH-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-ATH-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Protein family/group databases

MEROPSiT01.011.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta type-7-B (EC:3.4.25.1)
Alternative name(s):
20S proteasome beta subunit B-2
Proteasome component FC
Proteasome subunit beta type-2
Gene namesi
Name:PBB2
Synonyms:PRCFC
Ordered Locus Names:At5g40580
ORF Names:MNF13.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G40580.
TAIRilocus:2168718. AT5G40580.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000428321 – 37Removed in mature formAdd BLAST37
ChainiPRO_000004283338 – 274Proteasome subunit beta type-7-BAdd BLAST237

Keywords - PTMi

Zymogen

Proteomic databases

PaxDbiQ7DLS1.
PRIDEiQ7DLS1.

Expressioni

Gene expression databases

ExpressionAtlasiQ7DLS1. baseline and differential.
GenevisibleiQ7DLS1. AT.

Interactioni

Subunit structurei

Component of the 20S core complex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.2 Publications

Protein-protein interaction databases

BioGridi19307. 3 interactors.
IntActiQ7DLS1. 1 interactor.
STRINGi3702.AT5G40580.1.

Structurei

3D structure databases

ProteinModelPortaliQ7DLS1.
SMRiQ7DLS1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0173. Eukaryota.
COG0638. LUCA.
HOGENOMiHOG000182856.
InParanoidiQ7DLS1.
KOiK02739.
OMAiDLCVIRK.
OrthoDBiEOG09360IM6.
PhylomeDBiQ7DLS1.

Family and domain databases

Gene3Di3.60.20.10. 1 hit.
InterProiView protein in InterPro
IPR029055. Ntn_hydrolases_N.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
PfamiView protein in Pfam
PF00227. Proteasome. 1 hit.
PRINTSiPR00141. PROTEASOME.
SUPFAMiSSF56235. SSF56235. 1 hit.
PROSITEiView protein in PROSITE
PS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q7DLS1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSQSSVDIPP KGGFSFDLCK RNDMLTQKGL KAPSFLKTGT TIVGLIFKDG
60 70 80 90 100
VILGADTRAT EGPIVADKNC EKIHYMAPNI YCCGAGTAAD TEAVTDMVSS
110 120 130 140 150
QLRLHRYQTG RDSRVVTALT LLKKHLFSYQ GHVSAALVLG GVDITGPHLH
160 170 180 190 200
TIYPHGSTDT LPFATMGSGS LAAMSVFEAK YKEGLTRDEG IKLVAEAICS
210 220 230 240 250
GIFNDLGSGS NVDICVITKG HKEYLRNYME PNPRTYVSSK GYSFTKKTEV
260 270
LLTKITPLLE RVEIVEVAGE AMEE
Length:274
Mass (Da):29,617
Last modified:November 22, 2005 - v2
Checksum:i4AD31807A97D4C51
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti173A → V in AAM13010 (PubMed:14593172).Curated1
Sequence conflicti173A → V in AAM47910 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043531 mRNA. Translation: AAC32067.1.
AB009052 Genomic DNA. Translation: BAB08528.1.
CP002688 Genomic DNA. Translation: AED94567.1.
CP002688 Genomic DNA. Translation: AED94568.1.
AY093011 mRNA. Translation: AAM13010.1.
AY114591 mRNA. Translation: AAM47910.1.
AY087750 mRNA. Translation: AAM65286.1.
Y13177 mRNA. Translation: CAA73620.1.
PIRiT51979.
RefSeqiNP_198874.1. NM_123422.4. [Q7DLS1-1]
NP_851108.1. NM_180777.3. [Q7DLS1-1]
UniGeneiAt.47287.
At.66685.

Genome annotation databases

EnsemblPlantsiAT5G40580.1; AT5G40580.1; AT5G40580. [Q7DLS1-1]
AT5G40580.2; AT5G40580.2; AT5G40580. [Q7DLS1-1]
GeneIDi834056.
GrameneiAT5G40580.1; AT5G40580.1; AT5G40580.
AT5G40580.2; AT5G40580.2; AT5G40580.
KEGGiath:AT5G40580.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPSB7B_ARATH
AccessioniPrimary (citable) accession number: Q7DLS1
Secondary accession number(s): O23709, Q8LAK8, Q8RWL9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: September 27, 2017
This is version 110 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families