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Protein

Proteasome subunit beta type-7-B

Gene

PBB2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei38NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiR-ATH-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-ATH-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-ATH-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-ATH-382556 ABC-family proteins mediated transport
R-ATH-4641258 Degradation of DVL
R-ATH-5632684 Hedgehog 'on' state
R-ATH-5668541 TNFR2 non-canonical NF-kB pathway
R-ATH-5689603 UCH proteinases
R-ATH-5689880 Ub-specific processing proteases
R-ATH-6798695 Neutrophil degranulation
R-ATH-68949 Orc1 removal from chromatin
R-ATH-69017 CDK-mediated phosphorylation and removal of Cdc6
R-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation

Protein family/group databases

MEROPSiT01.011

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta type-7-B (EC:3.4.25.1)
Alternative name(s):
20S proteasome beta subunit B-2
Proteasome component FC
Proteasome subunit beta type-2
Gene namesi
Name:PBB2
Synonyms:PRCFC
Ordered Locus Names:At5g40580
ORF Names:MNF13.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G40580
TAIRilocus:2168718 AT5G40580

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000428321 – 37Removed in mature formAdd BLAST37
ChainiPRO_000004283338 – 274Proteasome subunit beta type-7-BAdd BLAST237

Keywords - PTMi

Zymogen

Proteomic databases

PaxDbiQ7DLS1
PRIDEiQ7DLS1

Expressioni

Gene expression databases

ExpressionAtlasiQ7DLS1 baseline and differential
GenevisibleiQ7DLS1 AT

Interactioni

Subunit structurei

Component of the 20S core complex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.2 Publications

Protein-protein interaction databases

BioGridi19307, 3 interactors
IntActiQ7DLS1, 1 interactor
STRINGi3702.AT5G40580.1

Structurei

3D structure databases

ProteinModelPortaliQ7DLS1
SMRiQ7DLS1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0173 Eukaryota
COG0638 LUCA
HOGENOMiHOG000182856
InParanoidiQ7DLS1
KOiK02739
OMAiDLCVIRK
OrthoDBiEOG09360IM6
PhylomeDBiQ7DLS1

Family and domain databases

Gene3Di3.60.20.10, 1 hit
InterProiView protein in InterPro
IPR029055 Ntn_hydrolases_N
IPR000243 Pept_T1A_subB
IPR016050 Proteasome_bsu_CS
IPR001353 Proteasome_sua/b
IPR023333 Proteasome_suB-type
PfamiView protein in Pfam
PF00227 Proteasome, 1 hit
PRINTSiPR00141 PROTEASOME
SUPFAMiSSF56235 SSF56235, 1 hit
PROSITEiView protein in PROSITE
PS00854 PROTEASOME_BETA_1, 1 hit
PS51476 PROTEASOME_BETA_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q7DLS1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSQSSVDIPP KGGFSFDLCK RNDMLTQKGL KAPSFLKTGT TIVGLIFKDG
60 70 80 90 100
VILGADTRAT EGPIVADKNC EKIHYMAPNI YCCGAGTAAD TEAVTDMVSS
110 120 130 140 150
QLRLHRYQTG RDSRVVTALT LLKKHLFSYQ GHVSAALVLG GVDITGPHLH
160 170 180 190 200
TIYPHGSTDT LPFATMGSGS LAAMSVFEAK YKEGLTRDEG IKLVAEAICS
210 220 230 240 250
GIFNDLGSGS NVDICVITKG HKEYLRNYME PNPRTYVSSK GYSFTKKTEV
260 270
LLTKITPLLE RVEIVEVAGE AMEE
Length:274
Mass (Da):29,617
Last modified:November 22, 2005 - v2
Checksum:i4AD31807A97D4C51
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti173A → V in AAM13010 (PubMed:14593172).Curated1
Sequence conflicti173A → V in AAM47910 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF043531 mRNA Translation: AAC32067.1
AB009052 Genomic DNA Translation: BAB08528.1
CP002688 Genomic DNA Translation: AED94567.1
CP002688 Genomic DNA Translation: AED94568.1
AY093011 mRNA Translation: AAM13010.1
AY114591 mRNA Translation: AAM47910.1
AY087750 mRNA Translation: AAM65286.1
Y13177 mRNA Translation: CAA73620.1
PIRiT51979
RefSeqiNP_198874.1, NM_123422.4 [Q7DLS1-1]
NP_851108.1, NM_180777.3 [Q7DLS1-1]
UniGeneiAt.47287
At.66685

Genome annotation databases

EnsemblPlantsiAT5G40580.1; AT5G40580.1; AT5G40580 [Q7DLS1-1]
AT5G40580.2; AT5G40580.2; AT5G40580 [Q7DLS1-1]
GeneIDi834056
GrameneiAT5G40580.1; AT5G40580.1; AT5G40580 [Q7DLS1-1]
AT5G40580.2; AT5G40580.2; AT5G40580 [Q7DLS1-1]
KEGGiath:AT5G40580

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPSB7B_ARATH
AccessioniPrimary (citable) accession number: Q7DLS1
Secondary accession number(s): O23709, Q8LAK8, Q8RWL9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: April 25, 2018
This is version 112 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health