Reviewed, UniProtKB/Swiss-Prot Q7DDL5 (CYSK_NEIMB)

Names and origin

Protein names

Recommended name:
    Cysteine synthase
      Short name=CSase
    EC=2.5.1.47
Alternative name(s):
    O-acetylserine sulfhydrylase
    O-acetylserine (thiol)-lyase
      Short name=OAS-TL

Gene names

Name:	cysK
Ordered Locus Names:	NMB0763

Organism

Neisseria meningitidis serogroup B [Complete proteome] [HAMAP]

Taxonomic identifier

491 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	O(3)-acetyl-L-serine + H2S = L-cysteine + acetate.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.
Miscellaneous	Present in outer membrane vesicle formulations which are used as vaccines in human.
Sequence similarities	Belongs to the cysteine synthase/cystathionine beta-synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process from serine Inferred from electronic annotation. Source: InterPro
Molecular function	cysteine synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 310	310	Cysteine synthase		PRO_0000320266
Regions
	Region	179 – 183	5	Pyridoxal phosphate binding By similarity
Sites
	Binding site	74	1	Pyridoxal phosphate By similarity
	Binding site	267	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	44	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7DDL5-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: FE9C74BF7C2AFD7A	FASTA	310	32,821
	10 20 30 40 50 60 MKIANSITEL IGNTPLVKLN RLTEGLKAEV AVKLEFFNPG SSVKDRIAEA MIEGAEKAGK 70 80 90 100 110 120 INKNTVIVEA TSGNTGVGLA MVCAARGYKL AITMPESMSK ERKMLLRAFG AELILTPAAE 130 140 150 160 170 180 GMAGAIAKAK SLVDAHPDTY FMPRQFDNEA NPEVHRKTTA EEIWRDTDGK VDVFVAGVGT 190 200 210 220 230 240 GGTITGVGEV LKKYKPEVKV VAVEPEASPV LSGGEKGPHP IQGIGAGFIP TVLNTKIYDS 250 260 270 280 290 300 ITKVSNEAAF ETARAIAEKE GILVGISSGA AVWSALQLAK QPENEGKLIV VLLPSYGERY 310 LSTPLFADLA

References

[1]	"Complete genome sequence of Neisseria meningitidis serogroup B strain MC58." Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E., Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C., Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H., Salzberg S.L., White O. , Fleischmann R.D., Dougherty B.A., Mason T.M., Ciecko A., Parksey D.S., Blair E., Cittone H., Clark E.B., Cotton M.D., Utterback T.R., Khouri H.M., Qin H., Vamathevan J.J., Gill J., Scarlato V., Masignani V., Pizza M., Grandi G., Sun L., Smith H.O., Fraser C.M., Moxon E.R., Rappuoli R., Venter J.C. Science 287:1809-1815(2000) [PubMed: 10710307] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MC58 / Serogroup B.
[2]	"Characterization of the protein content of a meningococcal outer membrane vesicle vaccine by polyacrylamide gel electrophoresis and mass spectrometry." Vipond C., Wheeler J.X., Jones C., Feavers I.M., Suker J. Hum. Vaccin. 1:80-84(2005) [PubMed: 17038831] [Abstract] Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[3]	"Proteomic analysis of a meningococcal outer membrane vesicle vaccine prepared from the group B strain NZ98/254." Vipond C., Suker J., Jones C., Tang C., Feavers I.M., Wheeler J.X. Proteomics 6:3400-3413(2006) [PubMed: 16645985] [Abstract] Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE002098 Genomic DNA. Translation: AAF41176.1.
PIR	H81161.
RefSeq	NP_273805.1.
3D structure databases
HSSP	HSSP built from PDB template 1O58 based on UniProtKB Q9WZD3.
ModBase	Search...
Genome annotation databases
GeneID	902878.
GenomeReviews	Gene locus NMB0763 in contig AE002098_GR.
KEGG	nme:NMB0763.
TIGR	NMB0763.
Phylogenomic databases
HOGENOM	Q7DDL5.
OMA	MSIERRC.
Enzyme and pathway databases
BioCyc	NMEN122586:NMB_0763-MON.
BRENDA	2.5.1.47. 293828.
Family and domain databases
InterPro	IPR001216. Cys_synth_BS. IPR005856. Cys_synthKM. IPR005859. CysK. IPR001926. PyrdxlP-dep_enz_bsu. [Graphical view]
Pfam	PF00291. PALP. 1 hit. [Graphical view]
TIGRFAMs	TIGR01139. cysK. 1 hit. TIGR01136. cysKM. 1 hit.
PROSITE	PS00901. CYS_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSK_NEIMB

Accession

Primary (citable) accession number: Q7DDL5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)