Reviewed, UniProtKB/Swiss-Prot Q7DBF9 (UDG_ECO57)

Names and origin

Protein names

Recommended name:
    UDP-glucose 6-dehydrogenase
      Short name=UDP-Glc dehydrogenase
      Short name=UDP-GlcDH
      Short name=UDPGDH
    EC=1.1.1.22

Gene names

Name:	ugd
Ordered Locus Names:	Z3190, ECs2829

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	UDP-glucose + 2 NAD(+) + H(2)O = UDP-glucuronate + 2 NADH.
Pathway	Nucleotide-sugar biosynthesis; UDP-glucuronate biosynthesis; UDP-glucuronate from UDP-glucose: step 1/1. Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.
Post-translational modification	Phosphorylated on a tyrosine residue. It results in a significant increase of the dehydrogenase activity By similarity.
Sequence similarities	Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	UDP-glucose 6-dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	UDP-glucose 6-dehydrogenase		PRO_0000074042
Regions
	Nucleotide binding	2 – 19	18	NAD Potential
Sites
	Active site	253	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7DBF9-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 44492C5BF394D2B9	FASTA	388	43,713
	10 20 30 40 50 60 MKITISGTGY VGLSNGLLIA QNHEVVALDI LPSRVAMLND RISPIVDKEI QQFLQSDKIH 70 80 90 100 110 120 FNATLDKNEA YRDADYVIIA TPTDYDPKTN YFNTSSVESV IKDVVEINPY AVMVIKSTVP 130 140 150 160 170 180 VGFTEAMHKK YRTENIIFSP EFLREGKALY DNLHPSRIVI GERSERAERF AALLQEGAIK 190 200 210 220 230 240 QNIPTLFTDS TEAEAIKLFA NTYLAMRVAY FNELDSYAES LGLNSRQIIE GVCLDPRIGN 250 260 270 280 290 300 HYNNPSFGYG GYCLPKDTKQ LLANYQSVPN NLISAIVDAN RTRKDFIADA ILSRKPQVVG 310 320 330 340 350 360 IYRLIMKSGS DNFRASSIQG IMKRIKAKGV EVIIYEPVMK EDSFFNSRLE RDLATFKQQA 370 380 DVIISNRMAE ELRDVADKVY TRDLFGSD

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE005174 Genomic DNA. Translation: AAG57087.1. BA000007 Genomic DNA. Translation: BAB36252.1.
RefSeq	NP_288533.1. NP_310856.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	913058. 962086.
GenomeReviews	Gene locus ECs2829 in contig BA000007_GR. Gene locus Z3190 in contig AE005174_GR.
KEGG	ece:Z3190. ecs:ECs2829.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q7DBF9.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS2829-MON.
Family and domain databases
InterPro	IPR016040. NAD(P)-bd. IPR017476. Nucleotide_sugar_DH. IPR014027. UDP-Glc/GDP-Man_DHase_C. IPR014026. UDP-Glc/GDP-Man_DHase_dimer. IPR014028. UDP-Glc/GDP-Man_DHase_dimer-bd. IPR001732. UDP-Glc/GDP-Man_DHase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:3.40.50.1870. UDP-Glc/GDP-Man_DH_C. 1 hit.
PANTHER	PTHR11374. UDPG_MGDP_DH_Creg. 1 hit.
Pfam	PF00984. UDPG_MGDP_dh. 1 hit. PF03720. UDPG_MGDP_dh_C. 1 hit. PF03721. UDPG_MGDP_dh_N. 1 hit. [Graphical view]
BLOCKS	Search...
ProtoNet	Search...

Entry information

Entry name

UDG_ECO57

Accession

Primary (citable) accession number: Q7DBF9
Secondary accession number(s): Q7ACQ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	July 5, 2004
Last modified:	November 4, 2008
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)