Q7DAK6 (TRPG_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Anthranilate synthase component 2
EC=4.1.3.27

Alternative name(s):
Anthranilate synthase component II
Glutamine amido-transferase

Gene names

Name:	trpG
Ordered Locus Names:	Rv0013, MT0016

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	232 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.
Subunit structure	Tetramer of two components I and two components II By similarity.
Miscellaneous	Component I catalyzes the formation of anthranilate using ammonia rather than glutamine, whereas component II provides glutamine amidotransferase activity By similarity. Was identified as a high-confidence drug target.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Domain	Glutamine amidotransferase
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW tryptophan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from direct assay. Source: MTBBASE
Molecular function	anthranilate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 232	232	Anthranilate synthase component 2		PRO_0000390894
Regions
	Domain	2 – 198	197	Glutamine amidotransferase type-1
Sites
	Active site	83	1	By similarity
	Active site	172	1	By similarity
	Active site	174	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7DAK6 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: A074EA1C2681AC0D	FASTA	232	24,627
	10 20 30 40 50 60 MRILVVDNYD SFVFNLVQYL GQLGIEAEVW RNDDHRLSDE AAVAGQFDGV LLSPGPGTPE 70 80 90 100 110 120 RAGASVSIVH ACAAAHTPLL GVCLGHQAIG VAFGATVDRA PELLHGKTSS VFHTNVGVLQ 130 140 150 160 170 180 GLPDPFTATR YHSLTILPKS LPAVLRVTAR TSSGVIMAVQ HTGLPIHGVQ FHPESILTEG 190 200 210 220 230 GHRILANWLT CCGWTQDDTL VRRLENEVLT AISPHFPTST ASAGEATGRT SA

References

[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis." Raman K., Yeturu K., Chandra N. BMC Syst. Biol. 2:109-109(2008) [PubMed: 19099550] [Abstract] Cited for: IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842572 Genomic DNA. Translation: CAE55234.1. AE000516 Genomic DNA. Translation: AAK44238.1.
PIR	C70699.
RefSeq	NP_334424.1. NC_002755.2. YP_177615.1. NC_000962.2.
3D structure databases
ProteinModelPortal	Q7DAK6.
ModBase	Search...
Protein family/group databases
MEROPS	C26.A19.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000000103; EBMYCP00000000103; EBMYCG00000000103. EBMYCT00000070459; EBMYCP00000068518; EBMYCG00000070454.
GeneID	885955. 922462.
GenomeReviews	Gene locus MT0016 in contig AE000516_GR. Gene locus Rv0013 in contig AL123456_GR.
KEGG	mtc:MT0016. mtu:Rv0013.
PATRIC	18121762. VBIMycTub22151_0017.
TIGR	MT0016.
Organism-specific databases
TubercuList	Rv0013.
Phylogenomic databases
GeneTree	EBGT00050000015859.
HOGENOM	HBG292341.
OMA	EDSTIMA.
PhylomeDB	Q7DAK6.
ProtClustDB	PRK07765.
Family and domain databases
InterPro	IPR017926. GATASE_1. IPR006221. TrpG_papA. [Graphical view]
KO	K01664.
Pfam	PF00117. GATase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00566. TrpG_papA. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRPG_MYCTU

Accession

Primary (citable) accession number: Q7DAK6
Secondary accession number(s): Q79G16

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 19, 2010
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program