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Protein

3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase

Gene

fabA

Organism
Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Necessary for the introduction of cis unsaturation into fatty acids. Catalyzes the dehydration of (3R)-3-hydroxydecanoyl-ACP to E-(2)-decenoyl-ACP and then its isomerization to Z-(3)-decenoyl-ACP. Can catalyze the dehydratase reaction for beta-hydroxyacyl-ACPs with saturated chain lengths up to 16:0, being most active on intermediate chain length.UniRule annotation

Catalytic activityi

A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl-carrier protein] + H2O.UniRule annotation
(3R)-3-hydroxydecanoyl-[acyl-carrier-protein] = trans-dec-2-enoyl-[acyl-carrier-protein] + H2O.UniRule annotation
Trans-dec-2-enoyl-[acyl-carrier-protein] = cis-dec-3-enoyl-[acyl-carrier-protein].UniRule annotation

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei71UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciAGRO:ATU0151-MONOMER.
UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
3-hydroxydecanoyl-[acyl-carrier-protein] dehydrataseUniRule annotation (EC:4.2.1.59UniRule annotation)
Alternative name(s):
3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabAUniRule annotation
Beta-hydroxydecanoyl thioester dehydraseUniRule annotation
Trans-2-decenoyl-[acyl-carrier-protein] isomeraseUniRule annotation (EC:5.3.3.14UniRule annotation)
Gene namesi
Name:fabAUniRule annotation
Ordered Locus Names:Atu0151
ORF Names:AGR_C_246
OrganismiAgrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58))
Taxonomic identifieri176299 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex
Proteomesi
  • UP000000813 Componenti: Chromosome circular

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002011691 – 1713-hydroxydecanoyl-[acyl-carrier-protein] dehydrataseAdd BLAST171

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi176299.Atu0151.

Structurei

3D structure databases

ProteinModelPortaliQ7D235.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thioester dehydratase family. FabA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108RKI. Bacteria.
COG0764. LUCA.
HOGENOMiHOG000277828.
KOiK01716.
OMAiFFDCHFK.

Family and domain databases

CDDicd01287. FabA. 1 hit.
Gene3Di3.10.129.10. 1 hit.
HAMAPiMF_00405. FabA. 1 hit.
InterProiIPR010083. FabA.
IPR013114. FabA_FabZ.
IPR029069. HotDog_dom.
[Graphical view]
PfamiPF07977. FabA. 1 hit.
[Graphical view]
SUPFAMiSSF54637. SSF54637. 1 hit.
TIGRFAMsiTIGR01749. fabA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q7D235-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATRQSSYNY EEILSCGRGE LFGPGNAQLP LPPMLMVHRI TEISETGGAF
60 70 80 90 100
DKGFIRAEYD VSPDDWYFPC HFQGNPIMPG CLGLDGMWQL TGFFLGWLGE
110 120 130 140 150
EGRGMALSTG EVKFKGMVRP NTKLLQYGID FKRVMRGRLV LGTADGWLKA
160 170
DGETIYQASD LRVGLSKEKA A
Length:171
Mass (Da):18,951
Last modified:January 15, 2008 - v2
Checksum:i1A69574B2E7A0F00
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE007869 Genomic DNA. Translation: AAK85972.2.
RefSeqiNP_353187.2. NC_003062.2.
WP_006309974.1. NC_003062.2.

Genome annotation databases

EnsemblBacteriaiAAK85972; AAK85972; Atu0151.
GeneIDi1132189.
KEGGiatu:Atu0151.
PATRICi20809982. VBIAgrTum91616_0142.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE007869 Genomic DNA. Translation: AAK85972.2.
RefSeqiNP_353187.2. NC_003062.2.
WP_006309974.1. NC_003062.2.

3D structure databases

ProteinModelPortaliQ7D235.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi176299.Atu0151.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK85972; AAK85972; Atu0151.
GeneIDi1132189.
KEGGiatu:Atu0151.
PATRICi20809982. VBIAgrTum91616_0142.

Phylogenomic databases

eggNOGiENOG4108RKI. Bacteria.
COG0764. LUCA.
HOGENOMiHOG000277828.
KOiK01716.
OMAiFFDCHFK.

Enzyme and pathway databases

UniPathwayiUPA00094.
BioCyciAGRO:ATU0151-MONOMER.

Family and domain databases

CDDicd01287. FabA. 1 hit.
Gene3Di3.10.129.10. 1 hit.
HAMAPiMF_00405. FabA. 1 hit.
InterProiIPR010083. FabA.
IPR013114. FabA_FabZ.
IPR029069. HotDog_dom.
[Graphical view]
PfamiPF07977. FabA. 1 hit.
[Graphical view]
SUPFAMiSSF54637. SSF54637. 1 hit.
TIGRFAMsiTIGR01749. fabA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFABA_AGRFC
AccessioniPrimary (citable) accession number: Q7D235
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: January 15, 2008
Last modified: October 5, 2016
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.