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Protein

Chorismate synthase

Gene

aroC

Organism
Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis. This reaction introduces a second double bond into the aromatic ring system.UniRule annotation

Catalytic activityi

5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate.UniRule annotation

Cofactori

FMNH2UniRule annotationNote: Reduced FMN (FMNH(2)).UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 7 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase 2 (aroQ2), 3-dehydroquinate dehydratase 1 (aroQ1)
  4. Shikimate dehydrogenase (NADP(+)) (aroE), Shikimate dehydrogenase (NADP(+)) (aroE), Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei48 – 481NADPUniRule annotation
Binding sitei54 – 541NADPUniRule annotation
Binding sitei288 – 2881FMN; via amide nitrogenUniRule annotation
Binding sitei329 – 3291FMNUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi131 – 1333FMNUniRule annotation
Nucleotide bindingi243 – 2442FMNUniRule annotation
Nucleotide bindingi303 – 3075FMNUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Enzyme and pathway databases

BioCyciAGRO:ATU0754-MONOMER.
UniPathwayiUPA00053; UER00090.

Names & Taxonomyi

Protein namesi
Recommended name:
Chorismate synthaseUniRule annotation (EC:4.2.3.5UniRule annotation)
Short name:
CSUniRule annotation
Alternative name(s):
5-enolpyruvylshikimate-3-phosphate phospholyaseUniRule annotation
Gene namesi
Name:aroCUniRule annotation
Ordered Locus Names:Atu0754
ORF Names:AGR_C_1368
OrganismiAgrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58))
Taxonomic identifieri176299 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex
Proteomesi
  • UP000000813 Componenti: Chromosome circular

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 365365Chorismate synthasePRO_1000059307Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi176299.Atu0754.

Structurei

3D structure databases

ProteinModelPortaliQ7D0R6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the chorismate synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D10. Bacteria.
COG0082. LUCA.
HOGENOMiHOG000060335.
KOiK01736.
OMAiMLSINAV.

Family and domain databases

CDDicd07304. Chorismate_synthase. 1 hit.
Gene3Di3.60.150.10. 1 hit.
HAMAPiMF_00300. Chorismate_synth. 1 hit.
InterProiIPR000453. Chorismate_synth.
IPR020541. Chorismate_synthase_CS.
[Graphical view]
PANTHERiPTHR21085. PTHR21085. 1 hit.
PfamiPF01264. Chorismate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001456. Chorismate_synth. 1 hit.
SUPFAMiSSF103263. SSF103263. 1 hit.
TIGRFAMsiTIGR00033. aroC. 1 hit.
PROSITEiPS00787. CHORISMATE_SYNTHASE_1. 1 hit.
PS00788. CHORISMATE_SYNTHASE_2. 1 hit.
PS00789. CHORISMATE_SYNTHASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q7D0R6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHNSFGHLF RVTTWGESHG PSLGCVVDGC PPGIRFTLAE VQHWMDKRKP
60 70 80 90 100
GQSRFVTQRR EDDIVKVLSG VMLDEDGETM TTTGTPISML IENTDQRSKD
110 120 130 140 150
YGEIARRFRP GHADFTYDLK YGIRDYRGGG RSSARETAAR VAAGAIARKV
160 170 180 190 200
VPSLNVRGAL VQIGKHKINR DNWDWDQVDQ NPFFCPDPEM VPVWEEYLDG
210 220 230 240 250
IRKSGSSIGA VVEVVAEGVP AGIGAPIYAK LDQDIASSLM SINAVKGVEI
260 270 280 290 300
GEGFASAELS GEENADQMRM GNDGKPIFLS NHAGGILGGI ATGEPVIARF
310 320 330 340 350
AIKPTSSILT ERLSIDTDGN NVDVRTKGRH DPCVGIRAVP IGEAMIACTV
360
ADHYLRDRGQ TGRLK
Length:365
Mass (Da):39,634
Last modified:January 15, 2008 - v2
Checksum:i4EEDD281A42307CE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE007869 Genomic DNA. Translation: AAK86563.2.
RefSeqiNP_353778.2. NC_003062.2.
WP_010971124.1. NC_003062.2.

Genome annotation databases

EnsemblBacteriaiAAK86563; AAK86563; Atu0754.
GeneIDi1132792.
KEGGiatu:Atu0754.
PATRICi20811215. VBIAgrTum91616_0751.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE007869 Genomic DNA. Translation: AAK86563.2.
RefSeqiNP_353778.2. NC_003062.2.
WP_010971124.1. NC_003062.2.

3D structure databases

ProteinModelPortaliQ7D0R6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi176299.Atu0754.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAK86563; AAK86563; Atu0754.
GeneIDi1132792.
KEGGiatu:Atu0754.
PATRICi20811215. VBIAgrTum91616_0751.

Phylogenomic databases

eggNOGiENOG4105D10. Bacteria.
COG0082. LUCA.
HOGENOMiHOG000060335.
KOiK01736.
OMAiMLSINAV.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00090.
BioCyciAGRO:ATU0754-MONOMER.

Family and domain databases

CDDicd07304. Chorismate_synthase. 1 hit.
Gene3Di3.60.150.10. 1 hit.
HAMAPiMF_00300. Chorismate_synth. 1 hit.
InterProiIPR000453. Chorismate_synth.
IPR020541. Chorismate_synthase_CS.
[Graphical view]
PANTHERiPTHR21085. PTHR21085. 1 hit.
PfamiPF01264. Chorismate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001456. Chorismate_synth. 1 hit.
SUPFAMiSSF103263. SSF103263. 1 hit.
TIGRFAMsiTIGR00033. aroC. 1 hit.
PROSITEiPS00787. CHORISMATE_SYNTHASE_1. 1 hit.
PS00788. CHORISMATE_SYNTHASE_2. 1 hit.
PS00789. CHORISMATE_SYNTHASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAROC_AGRFC
AccessioniPrimary (citable) accession number: Q7D0R6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: January 15, 2008
Last modified: September 7, 2016
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.