Q7CXF9 (KITH_AGRT5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine kinase
EC=2.7.1.21

Gene names

Name:	tdk
Ordered Locus Names:	Atu2282
ORF Names:	AGR_C_4147

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970) [Reference proteome] [HAMAP]

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium › Agrobacterium tumefaciens complex › Agrobacterium fabrum

Protein attributes

Sequence length	193 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + thymidine = ADP + thymidine 5'-phosphate. HAMAP-Rule MF_00124
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00124.
Sequence similarities	Belongs to the thymidine kinase family.
Sequence caution	The sequence AAK88024.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	DNA synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP thymidine kinase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 193	193	Thymidine kinase HAMAP-Rule MF_00124		PRO_0000242786
Regions
	Nucleotide binding	9 – 16	8	ATP By similarity
	Nucleotide binding	87 – 90	4	ATP By similarity
Sites
	Active site	88	1	Proton acceptor Potential
	Metal binding	145	1	Zinc By similarity
	Metal binding	147	1	Zinc By similarity
	Metal binding	182	1	Zinc By similarity
	Metal binding	185	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7CXF9 [UniParc]. Last modified June 27, 2006. Version 2. Checksum: 3F80FBBEA039E1DC	FASTA	193	21,548
	10 20 30 40 50 60 MAKLYFNYST MNAGKSTMLL QASYNYQERG MRTLIFTAAF DDRAGVGRVA SRIGLSSEAR 70 80 90 100 110 120 TFDENTDLFA EVAALHAQAP VACVFVDEAN FLSEHHVWQL ANIADRLNIP VMTYGLRTDF 130 140 150 160 170 180 QGKLFPASKE LLAIADELRE IRTICHCGRK ATMVARFDSE GKVVTEGAQI EVGGNGKYVS 190 FCRRHWVETF NCS

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE007869 Genomic DNA. Translation: AAK88024.2. Different initiation.
PIR	AI2856. G97633.
RefSeq	NP_355239.2. NC_003062.2.
3D structure databases
ProteinModelPortal	Q7CXF9.
ModBase	Search...
Protein-protein interaction databases
STRING	176299.Atu2282.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1134320.
KEGG	atu:Atu2282.
PATRIC	20814349. VBIAgrTum91616_2294.
Phylogenomic databases
eggNOG	COG1435.
HOGENOM	HOG000076391.
KO	K00857.
OMA	FRYGAMN.
ProtClustDB	PRK04296.
Family and domain databases
HAMAP	MF_00124. Thymidine_kinase.
InterPro	IPR001267. Thymidine_kinase. IPR020634. Thymidine_kinase_subgr. [Graphical view]
PANTHER	PTHR11441. PTHR11441. 1 hit.
Pfam	PF00265. TK. 1 hit. [Graphical view]
PIRSF	PIRSF035805. TK_cell. 1 hit.
PROSITE	PS00603. TK_CELLULAR_TYPE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KITH_AGRT5

Accession

Primary (citable) accession number: Q7CXF9
Secondary accession number(s): Q8UD45

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	June 27, 2006
Last modified:	May 1, 2013
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program