Catalase-peroxidase - Agrobacterium tumefaciens (strain C58 / ATCC 33970)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	Atu4642
ORF Names:	AGR_L_481

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970)

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	723 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H₂O₂ = oxidized donor + 2 H₂O. HAMAP MF_01961 2 H₂O₂ = O₂ + 2 H₂O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 723

723

Catalase-peroxidase HAMAP MF_01961

PRO_0000354716

Sites

Active site

98

1

Proton acceptor By similarity

Metal binding

266

1

Iron (heme axial ligand) By similarity

Site

94

1

Transition state stabilizer By similarity

Amino acid modifications

Cross-link

97 ↔ 225

Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-251) By similarity

Cross-link

225 ↔ 251

Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-97) By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q7CVH1 [UniParc].

Last modified January 15, 2008. Version 2.
Checksum: DFCDD8568BA90211
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FASTA

723

78,713

        10         20         30         40         50         60 
MDATSKPAGK CPVMHGGNTA SGKSVTEWWP NALNLDILHQ HDTKTNPLGT SFNYREALKT 

        70         80         90        100        110        120 
LDVEALKADL RALMTDSQEW WPADWGSYVG MMARVTWHAA GSYRVTDGRG GANTGNQRFA 

       130        140        150        160        170        180 
PLNSWPDNVN TDKGRRLLWP IKKKYGNKIS WADLIALAGT IAYDVAGLKT FGFAFGREDI 

       190        200        210        220        230        240 
WAPEKDTYWG DEKEWLAPSD GRYGDVSKPE TLENPLAAVQ MGLIYVNPEG VNGKSDPLAT 

       250        260        270        280        290        300 
AAQMRETFAR MGMDDEETVA LTAGGHTIGK SHGNGSAANL SPDPEAAGPE YQGLGWINTK 

       310        320        330        340        350        360 
GRGIGRDTVV SGIEGAWTSE PTKWDNGFFD MLFKHEWTLT HSPAGASQWA PITIAEEDKP 

       370        380        390        400        410        420 
VDVEDASIRT IPMMTDADMA LKVDPIYREI SLKFKDDQDH FSDVFARAWF KLTHRDMGPK 

       430        440        450        460        470        480 
SRYVGPDVPA EDLIWQDPIP AGSTSYDVAA VKAKIAASGL SVADLVSTAW DSARTFRGSD 

       490        500        510        520        530        540 
KRGGANGARI RLAPQKDWEG NEPARLSRVL SVLEPIARET GASIADVIVL AGNYGVEQAA 

       550        560        570        580        590        600 
KAAGFDIAVP FAAGRGDASA EQTDADSFAP LEPLADGFRN WVKKDYVVSP EELLLDRAQL 

       610        620        630        640        650        660 
LGLTAPELTV LIGGLRVIGA NYGGAAHGVF TDKPGALTTD FFTTLTDMAY SWVPTGNNLY 

       670        680        690        700        710        720 
EIRDRKTGAA RYSATRVDLV IGSNSILRAY AEVYAQDDNR EKFARDFIAA WTKVMNADRF 


DLI

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References

[1]	"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.
[2]	"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE007870 Genomic DNA. Translation: AAK88805.2.
RefSeq	NP_356020.2. NC_003063.2.
3D structure databases
HSSP	HSSP built from PDB template 1U2L based on UniProtKB P13029.
ProteinModelPortal	Q7CVH1.
SMR	Q7CVH1. Positions 24-722.
ModBase	Search...
Protein-protein interaction databases
STRING	Q7CVH1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1136516.
GenomeReviews	Gene locus Atu4642 in contig AE007870_GR.
KEGG	atu:Atu4642.
PATRIC	20818704. VBIAgrTum91616_4447.
Phylogenomic databases
eggNOG	COG0376.
HOGENOM	HBG285610.
OMA	WPNALNL.
PhylomeDB	Q7CVH1.
ProtClustDB	PRK15061.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. False negative. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_AGRT5

Accession

Primary (citable) accession number: Q7CVH1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	January 15, 2008
Last modified:	January 25, 2012
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents