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Protein

Guanine nucleotide exchange factor sopE2

Gene

sopE2

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Activator for CDC42 by directly engaging this Rho GTPase and acting as potent guanine nucleotide exchange factor (GEF). This activation results in actin cytoskeleton rearrangements and stimulates membrane ruffling, promoting bacterial entry into non-phagocytic cells. Also activates NF-kB, p38 and ERK kinases, which are known to be involved in the induction of IL-8 expression. Chaperone InvB is required for secretion, translocation and stabilization of intracellular levels of sopE2.2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation, Guanine-nucleotide releasing factor

Keywords - Biological processi

Virulence

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide exchange factor sopE2
Alternative name(s):
Effector protein sopE2
Toxin sopE2
Gene namesi
Name:sopE2
Ordered Locus Names:STM1855
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

  • Secreted

  • Note: Secreted via the type III secretion system 1 (SPI-1 TTSS).

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002207422 – 240Guanine nucleotide exchange factor sopE2Add BLAST239

Proteomic databases

PaxDbiQ7CQD4.
PRIDEiQ7CQD4.

Interactioni

Protein-protein interaction databases

IntActiQ7CQD4. 1 interactor.
STRINGi99287.STM1855.

Structurei

Secondary structure

1240
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi80 – 82Combined sources3
Helixi83 – 94Combined sources12
Helixi96 – 102Combined sources7
Helixi104 – 133Combined sources30
Helixi137 – 150Combined sources14
Beta strandi155 – 157Combined sources3
Beta strandi162 – 164Combined sources3
Helixi173 – 183Combined sources11
Helixi185 – 188Combined sources4
Helixi191 – 209Combined sources19
Helixi210 – 213Combined sources4
Beta strandi215 – 217Combined sources3
Helixi221 – 237Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R6ENMR-A73-240[»]
1R9KNMR-A69-240[»]
ProteinModelPortaliQ7CQD4.
SMRiQ7CQD4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ7CQD4.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni78 – 240GEF catalytic domainBy similarityAdd BLAST163

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4106EWC. Bacteria.
ENOG410XV0S. LUCA.
HOGENOMiHOG000028399.
KOiK13741.
OMAiLDIKSHA.

Family and domain databases

InterProiIPR005414. SopE.
IPR016019. SopE_GEF_dom.
IPR016018. SopE_N_dom.
[Graphical view]
PfamiPF05364. SecIII_SopE_N. 1 hit.
PF07487. SopE_GEF. 1 hit.
[Graphical view]
PIRSFiPIRSF034781. SecIII_sopE. 1 hit.
PRINTSiPR01593. SOPEPROTEIN.
SUPFAMiSSF81832. SSF81832. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q7CQD4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNITLSTQH YRIHRSDVEP VKEKTTEKDI FAKSITAVRN SFISLSTSLS
60 70 80 90 100
DRFSLHQQTD IPTTHFHRGN ASEGRAVLTS KTVKDFMLQK LNSLDIKGNA
110 120 130 140 150
SKDPAYARQT CEAILSAVYS NNKDQCCKLL ISKGVSITPF LKEIGEAAQN
160 170 180 190 200
AGLPGEIKNG VFTPGGAGAN PFVVPLIASA SIKYPHMFIN HNQQVSFKAY
210 220 230 240
AEKIVMKEVT PLFNKGTMPT PQQFQLTIEN IANKYLQNAS
Length:240
Mass (Da):26,448
Last modified:January 23, 2007 - v3
Checksum:iB4A113292FABD65F
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti125Q → H in strain: F98. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF200952 Genomic DNA. Translation: AAF63159.1.
AF217274 Genomic DNA. Translation: AAF91225.1.
AE006468 Genomic DNA. Translation: AAL20770.1.
RefSeqiNP_460811.1. NC_003197.1.
WP_000182072.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL20770; AAL20770; STM1855.
GeneIDi1253374.
KEGGistm:STM1855.
PATRICi32382243. VBISalEnt20916_1960.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF200952 Genomic DNA. Translation: AAF63159.1.
AF217274 Genomic DNA. Translation: AAF91225.1.
AE006468 Genomic DNA. Translation: AAL20770.1.
RefSeqiNP_460811.1. NC_003197.1.
WP_000182072.1. NC_003197.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R6ENMR-A73-240[»]
1R9KNMR-A69-240[»]
ProteinModelPortaliQ7CQD4.
SMRiQ7CQD4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ7CQD4. 1 interactor.
STRINGi99287.STM1855.

Proteomic databases

PaxDbiQ7CQD4.
PRIDEiQ7CQD4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL20770; AAL20770; STM1855.
GeneIDi1253374.
KEGGistm:STM1855.
PATRICi32382243. VBISalEnt20916_1960.

Phylogenomic databases

eggNOGiENOG4106EWC. Bacteria.
ENOG410XV0S. LUCA.
HOGENOMiHOG000028399.
KOiK13741.
OMAiLDIKSHA.

Miscellaneous databases

EvolutionaryTraceiQ7CQD4.

Family and domain databases

InterProiIPR005414. SopE.
IPR016019. SopE_GEF_dom.
IPR016018. SopE_N_dom.
[Graphical view]
PfamiPF05364. SecIII_SopE_N. 1 hit.
PF07487. SopE_GEF. 1 hit.
[Graphical view]
PIRSFiPIRSF034781. SecIII_sopE. 1 hit.
PRINTSiPR01593. SOPEPROTEIN.
SUPFAMiSSF81832. SSF81832. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSOPE2_SALTY
AccessioniPrimary (citable) accession number: Q7CQD4
Secondary accession number(s): Q9KIZ2, Q9L8K7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 83 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

SopE2 is highly conserved among Salmonella isolates of the SARA collection.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.