Q7CNQ9 (HPRT_STRP8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hypoxanthine-guanine phosphoribosyltransferase
Short name=HGPRT
Short name=HGPRTase
EC=2.4.2.8

Gene names

Name:	hpt
Ordered Locus Names:	spyM18_0013

Organism

Streptococcus pyogenes serotype M18 (strain MGAS8232) [Complete proteome] [HAMAP]

Taxonomic identifier

186103 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus › Streptococcus pyogenes › Streptococcus pyogenes serotype M18

Protein attributes

Sequence length	180 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate. GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.
Cofactor	Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein By similarity.
Pathway	Purine metabolism; IMP biosynthesis via salvage pathway; IMP from hypoxanthine: step 1/1.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family.

Ontologies

Keywords
Biological process	Purine salvage
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding Nucleotide-binding
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	IMP salvage Inferred from electronic annotation. Source: UniProtKB-UniPathway purine ribonucleoside salvage Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	guanine phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC hypoxanthine phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 180	180	Hypoxanthine-guanine phosphoribosyltransferase		PRO_0000139625
Regions
	Nucleotide binding	99 – 108	10	IMP By similarity
	Nucleotide binding	158 – 159	2	IMP By similarity
Sites
	Active site	103	1	Proton acceptor By similarity
	Metal binding	159	1	Magnesium By similarity
	Binding site	131	1	IMP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7CNQ9 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 5EC991DCC3524B8B	FASTA	180	20,725
	10 20 30 40 50 60 MLEQDIQKIL YSENDIIRKT KKLGEQLTKD YQEKNPLMIG VLKGSVPFMA ELMKHIDTHV 70 80 90 100 110 120 EIDFMVVSSY HGGTSSSGEV KILKDVDTNI EGRDIIIVED IIDTGRTLKY LRDMFKYRKA 130 140 150 160 170 180 NTIKIATLFD KPEGRVVKIE ADYVCYNIPN EFIVGFGLDY AENYRNLPYV GVLKEEVYSK

References

[1]

"Genome sequence and comparative microarray analysis of serotype M18 group A Streptococcus strains associated with acute rheumatic fever outbreaks." Smoot J.C., Barbian K.D., Van Gompel J.J., Smoot L.M., Chaussee M.S., Sylva G.L., Sturdevant D.E., Ricklefs S.M., Porcella S.F., Parkins L.D., Beres S.B., Campbell D.S., Smith T.M., Zhang Q., Kapur V., Daly J.A., Veasy L.G., Musser J.M. Proc. Natl. Acad. Sci. U.S.A. 99:4668-4673(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MGAS8232.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009949 Genomic DNA. Translation: AAL96846.1.
RefSeq	NP_606347.1. NC_003485.1.
3D structure databases
ProteinModelPortal	Q7CNQ9.
SMR	Q7CNQ9. Positions 3-178.
ModBase	Search...
Protein-protein interaction databases
STRING	186103.spyM18_0013.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL96846; AAL96846; spyM18_0013.
GeneID	995231.
KEGG	spm:spyM18_0013.
PATRIC	19746727. VBIStrPyo4396_0012.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0634.
HOGENOM	HOG000236520.
KO	K00760.
OMA	YSAVLCE.
ProtClustDB	CLSK883890.
Enzyme and pathway databases
UniPathway	UPA00591; UER00648.
Family and domain databases
InterPro	IPR005904. Hxn_phspho_trans. IPR000836. PRibTrfase_dom. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01203. HGPRTase. 1 hit.
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HPRT_STRP8

Accession

Primary (citable) accession number: Q7CNQ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2005
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program