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Protein

UDP-glucuronate:glycolipid 2-beta-glucuronosyltransferase

Gene

gumK

Organism
Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the transfer of a glucuronic acid (GlcA) residue from UDP-glucuronate to mannose-alpha-1,3-glucose-beta-1,4-glucose-P-P-polyisoprenyl to form the lipid-linked tetrasaccharide GlcA-Man-Glc2-PP-Pol, with a glucuronic acid-beta-mannose linkage. Is involved in the biosynthesis of the exopolysaccharide xanthan, since it catalyzes the fourth glycosylation step in the assembly of the pentasaccharide-P-P-polyisoprenyl repeating unit of xanthan.By similarity

Catalytic activityi

UDP-alpha-D-glucuronate + alpha-D-Man-(1->3)-beta-D-Glc-(1->4)-alpha-D-Glc-1-diphospho-ditrans,octacis-undecaprenol = UDP + beta-D-GlcA-(1->2)-alpha-D-Man-(1->3)-beta-D-Glc-(1->4)-alpha-D-Glc-1-diphospho-ditrans,octacis-undecaprenol.By similarity

Pathwayi: xanthan biosynthesis

This protein is involved in the pathway xanthan biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway xanthan biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei157Proton acceptorBy similarity1
Binding sitei292UDP-glucuronateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processCarbohydrate metabolism

Enzyme and pathway databases

BioCyciXCAM190485:G1FZM-2443-MONOMER
UniPathwayiUPA01017

Protein family/group databases

CAZyiGT70 Glycosyltransferase Family 70

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-glucuronate:glycolipid 2-beta-glucuronosyltransferase (EC:2.4.1.264By similarity)
Short name:
UDP-GlcA:glycolipid glucuronosyltransferase
Alternative name(s):
D-man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol 2-beta-glucuronyltransferase
Gene namesi
Name:gumK
Ordered Locus Names:XCC2445
OrganismiXanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)
Taxonomic identifieri190485 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas
Proteomesi
  • UP000001010 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004140201 – 400UDP-glucuronate:glycolipid 2-beta-glucuronosyltransferaseBy similarityAdd BLAST400

Interactioni

Protein-protein interaction databases

STRINGi190485.XCC2445

Structurei

3D structure databases

ProteinModelPortaliQ7CLR5
SMRiQ7CLR5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni230 – 231UDP-glucuronate bindingBy similarity2
Regioni272 – 273UDP-glucuronate bindingBy similarity2
Regioni306 – 310UDP-glucuronate bindingBy similarity5

Sequence similaritiesi

Belongs to the glycosyltransferase 70 family.Curated

Phylogenomic databases

eggNOGiENOG4108NQK Bacteria
ENOG410XTG8 LUCA
HOGENOMiHOG000219965
KOiK13659

Sequencei

Sequence statusi: Complete.

Q7CLR5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVSPAAPAS GIRRPCYLVL SAHDFRTPRR ANIHFITDQL ALRGTTRFFS
60 70 80 90 100
LRYSRLSRMK GDMRLPLDDT ANTVVSHNGV DCYLWRTTVH PFNTRRSWLR
110 120 130 140 150
PVEDAMFRWY AAHPPKQLLD WMRESDVIVF ESGIAVAFIE LAKRVNPAAK
160 170 180 190 200
LVYRASDGLS TINVASYIER EFDRVAPTLD VIALVSPAMA AEVASRDNVF
210 220 230 240 250
HVGHGVDHNL DQLGDPSPYA EGIHAVAVGS MLFDPEFFVV ASKAFPQVTF
260 270 280 290 300
HVIGSGMGRH PGYGDNVIVY GEMKHAQTIG YIKHARFGIA PYASEQVPVY
310 320 330 340 350
LADSSMKLLQ YDFFGLPAVC PNAVVGPYKS RFGYTPGNAD SVIAAITQAL
360 370 380 390 400
EAPRVRYRQC LNWSDTTDRV LDPRAYPETR LYPHPPTAAP QLSSEAALSH
Length:400
Mass (Da):44,438
Last modified:November 16, 2011 - v2
Checksum:i250231946AB7EB61
GO

Sequence cautioni

The sequence AAM41722 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE008922 Genomic DNA Translation: AAM41722.1 Different initiation.
RefSeqiNP_637798.2, NC_003902.1
WP_011037586.1, NC_003902.1

Genome annotation databases

EnsemblBacteriaiAAM41722; AAM41722; XCC2445
GeneIDi35545062
998848
KEGGixcc:XCC2445
PATRICifig|190485.4.peg.2609

Similar proteinsi

Entry informationi

Entry nameiGUMK_XANCP
AccessioniPrimary (citable) accession number: Q7CLR5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 16, 2011
Last sequence update: November 16, 2011
Last modified: March 28, 2018
This is version 60 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health