Q7CIZ3 (BGAL_YERPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-galactosidase
Short name=Beta-gal
EC=3.2.1.23

Alternative name(s):
Lactase

Gene names

Name:	lacZ
Ordered Locus Names:	YPO1654, y1817, YP_1785

Organism

Yersinia pestis

Taxonomic identifier

632 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	1060 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. HAMAP MF_01687
Cofactor	Binds 2 magnesium ions per monomer By similarity. HAMAP MF_01687 Binds 1 sodium ion per monomer By similarity. HAMAP MF_01687
Subunit structure	Homotetramer By similarity. HAMAP MF_01687
Sequence similarities	Belongs to the glycosyl hydrolase 2 family.

Ontologies

Keywords
Ligand	Magnesium Metal-binding Sodium
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	beta-galactosidase complex Inferred from electronic annotation. Source: InterPro
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC carbohydrate binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1060	1060	Beta-galactosidase HAMAP MF_01687		PRO_0000367015
Regions
	Region	553 – 556	4	Substrate binding By similarity
Sites
	Active site	477	1	Proton donor By similarity
	Active site	553	1	Nucleophile By similarity
	Metal binding	209	1	Sodium By similarity
	Metal binding	432	1	Magnesium 1 By similarity
	Metal binding	434	1	Magnesium 1 By similarity
	Metal binding	477	1	Magnesium 1 By similarity
	Metal binding	613	1	Magnesium 2 By similarity
	Metal binding	617	1	Sodium; via carbonyl oxygen By similarity
	Metal binding	620	1	Sodium By similarity
	Binding site	110	1	Substrate By similarity
	Binding site	209	1	Substrate By similarity
	Binding site	477	1	Substrate By similarity
	Binding site	620	1	Substrate By similarity
	Binding site	1035	1	Substrate By similarity
	Site	373	1	Transition state stabilizer By similarity
	Site	407	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7CIZ3 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 1142D7FC68E46697	FASTA	1,060	122,625
	10 20 30 40 50 60 MTSQEKVPLQ VQLSLPQILS RRDWENPQIT QYHRLEAHPP FHSWRDVESA QKDRPSPQQQ 70 80 90 100 110 120 TLNGLWSFSY FTQPEAVPEH WVRCDLAEAK PLPVPANWQL HGYDAPIYTN IQYPIPVNPP 130 140 150 160 170 180 RVPDLNPTGC YSRDFTLEPS WLASGKTRII FDGVSSAFYL WCNGQWVGYS QDSRLPAEFD 190 200 210 220 230 240 LTPYLQAGSN RIAVLVLRWS DGSYLEDQDM WRMSGIFRDV KLLHKPEIHL RDIHIMTHLS 250 260 270 280 290 300 PEFTSANLEV MAAVNIPSLQ LNDPQVTGSY QLRVQLWLAD KLVASLQQPL GTQAIDERGP 310 320 330 340 350 360 YTDRTQLVLR IDQPLLWSAE QPTLYRAVVS LLNHQQELIE AEAYDVGFRQ VAIHQGLLKI 370 380 390 400 410 420 NGKAVLIRGV NRHEHHPQTG QAIDEESLLQ DILLMKQHNF NAVRCSHYPN HPLWYRLCDR 430 440 450 460 470 480 YGLYVVDEAN IETHGMQPMS RLSDDPSWFS AFSERVTRMV QRDRNHPCII IWSLGNESGH 490 500 510 520 530 540 GATHDALYRW IKTNDPTRPV QYEGGGANTL ATDILCPMYA RVDEDQPFPA VPKWSIKKWI 550 560 570 580 590 600 GLPNESRPLI LCEYAHAMGN SFGGFARYWQ AFRQYPRLQG GFIWDWVDQS LTHHNDHGQP 610 620 630 640 650 660 YWAYGGDFGD TPNDRQFCMN GLVFPDRSPH PSLYEAQCAQ QFFQFSLLST TPLVINITSE 670 680 690 700 710 720 YLFRESDNEQ LYWRIMLEGE SVLEGSQPLN LSPESSQCYR LAEKLPTLNK PGQLWLNVEI 730 740 750 760 770 780 RQPKETPWSP AQHRSAWHQW RLPQPLFSPS SDLTNATAHY APQLQHNLQL QHDLQLQQDE 790 800 810 820 830 840 QHIKVTYQQQ CWQFSRQTGR LAQWWVADKP MLLRPLQDQF VRAPLDNDIG ISEATHIDPN 850 860 870 880 890 900 AWVERWKKAG MYQLQQRCLS LHVDHLSHSV QISAEYGYEF EQEPLLHSHW VYRFDRHGRM 910 920 930 940 950 960 TIDVNVRIAT SLPAPARIGM CCQLADISPT VEWLGLGPHE NYPDRQLAAQ YGHWSLPLEQ 970 980 990 1000 1010 1020 MHTAYIFPSE NGLRCNTHTL NYGRWTLTGD FHFGISRYST QQLMVTSHQH LLEPEEGTWL 1030 1040 1050 1060 NIDGFHMGVG GDDSWSPSVH IDDILTRETY QYQICWQYKV

References

[1]	"Genome sequence of Yersinia pestis KIM." Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P., Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D., Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A., Nilles M.L. , Matson J.S., Blattner F.R., Perry R.D. J. Bacteriol. 184:4601-4611(2002) [PubMed: 12142430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KIM5 / Biovar Mediaevalis.
[2]	"Genome sequence of Yersinia pestis, the causative agent of plague." Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G., Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L., Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M., Chillingworth T., Cronin A., Davies R.M. , Davis P., Dougan G., Feltwell T., Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S., Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:523-527(2001) [PubMed: 11586360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CO-92 / Biovar Orientalis.
[3]	"Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans." Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R. , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R. DNA Res. 11:179-197(2004) [PubMed: 15368893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 91001 / Biovar Mediaevalis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009952 Genomic DNA. Translation: AAM85385.1. AE017042 Genomic DNA. Translation: AAS62012.1. AL590842 Genomic DNA. Translation: CAL20299.1.
PIR	AI0201.
RefSeq	NP_669134.1. NC_004088.1. NP_993135.1. NC_005810.1. YP_002346664.1. NC_003143.1.
3D structure databases
ProteinModelPortal	Q7CIZ3.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q7CIZ3. 9 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1146764. 1174493. 2766512.
GenomeReviews	Gene locus y1817 in contig AE009952_GR. Gene locus YP_1785 in contig AE017042_GR. Gene locus YPO1654 in contig AL590842_GR.
KEGG	ype:YPO1654. ypk:y1817. ypm:YP_1785.
Phylogenomic databases
HOGENOM	HBG670643.
OMA	RDWENPA.
ProtClustDB	PRK09525.
Enzyme and pathway databases
BioCyc	YPES187410:Y1817-MONOMER.
Family and domain databases
HAMAP	MF_01687. Beta_gal. [Tree]
InterPro	IPR004199. B-gal_small/dom_5. IPR008979. Galactose-bd-like. IPR011013. Glyco_hydro-type_carb-bd. IPR014718. Glyco_hydro-type_carb-bd_sub. IPR006101. Glyco_hydro_2. IPR013812. Glyco_hydro_2/20_Ig-like. IPR023232. Glyco_hydro_2_AS. IPR023933. Glyco_hydro_2_beta_Galsidase. IPR023230. Glyco_hydro_2_CS. IPR006102. Glyco_hydro_2_Ig-like. IPR006104. Glyco_hydro_2_N. IPR006103. Glyco_hydro_2_TIM. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:2.60.40.320. Glyco_hydro_2/20_Ig-like. 2 hits. G3DSA:2.70.98.10. Glyco_hydro_42_D5. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
KO	K01190.
Pfam	PF02929. Bgal_small_N. 1 hit. PF00703. Glyco_hydro_2. 1 hit. PF02836. Glyco_hydro_2_C. 1 hit. PF02837. Glyco_hydro_2_N. 1 hit. [Graphical view]
PRINTS	PR00132. GLHYDRLASE2.
SMART	SM01038. Bgal_small_N. 1 hit. [Graphical view]
SUPFAM	SSF49785. Gal_bind_like. 1 hit. SSF74650. Gal_mut_like. 1 hit. SSF49303. Glyco_hydro_2Ig. 2 hits. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit. PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL_YERPE

Accession

Primary (citable) accession number: Q7CIZ3
Secondary accession number(s): Q74UE1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program