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Protein

Biotin synthase

Gene

bioB

Organism
Yersinia pestis
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. ATP-dependent dethiobiotin synthetase BioD (bioD), ATP-dependent dethiobiotin synthetase BioD 1 (bioD1), ATP-dependent dethiobiotin synthetase BioD 2 (bioD2), ATP-dependent dethiobiotin synthetase BioD (bioD)
  2. Biotin synthase (bioB), Biotin synthase (bioB)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi53Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi57Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi60Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi97Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi128Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi188Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi260Iron-sulfur 2 (2Fe-2S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processBiotin biosynthesis
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00078; UER00162

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:YPO1151, y3031, YP_1009
OrganismiYersinia pestis
Taxonomic identifieri632 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeYersinia
Proteomesi
  • UP000000815 Componenti: Chromosome
  • UP000001019 Componenti: Chromosome
  • UP000002490 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003817231 – 345Biotin synthaseAdd BLAST345

Proteomic databases

PaxDbiQ7CH65

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi187410.y3031

Structurei

3D structure databases

ProteinModelPortaliQ7CH65
SMRiQ7CH65
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZF Bacteria
COG0502 LUCA
HOGENOMiHOG000239957
KOiK01012
OMAiADRFCMG

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01694 BioB, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR010722 BATS_dom
IPR034416 BATS_domain_containing
IPR002684 Biotin_synth/BioAB
IPR024177 Biotin_synthase
IPR006638 Elp3/MiaB/NifB
IPR007197 rSAM
PANTHERiPTHR22976 PTHR22976, 1 hit
PfamiView protein in Pfam
PF06968 BATS, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF001619 Biotin_synth, 1 hit
SFLDiSFLDF00272 biotin_synthase, 1 hit
SFLDG01060 BATS_domain_containing, 1 hit
SFLDG01278 biotin_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00876 BATS, 1 hit
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00433 bioB, 1 hit

Sequencei

Sequence statusi: Complete.

Q7CH65-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATYHHWTVG QALALFDKPL LELLFEAQQV HRQHFDPRQV QVSTLLSIKT
60 70 80 90 100
GACPEDCKYC PQSSRYKTGL ESERLMQVEQ VLESAKKAKA AGSTRFCMGA
110 120 130 140 150
AWKNPHERDM PYLAKMVEGV KALGMETCMT LGSLSKQQAH RLADAGLDYY
160 170 180 190 200
NHNLDTSPEF YGSIITTRSY QERLDTLNEV RDAGIKVCSG GIVGLGETVR
210 220 230 240 250
DRAGLLVQLA NLPKPPESVP INMLVKVKGT PLENNAEVDA FEFIRTIAVA
260 270 280 290 300
RIMMPSSYVR LSAGREQMNE QTQAMCFMAG ANSIFYGCKL LTTPNPDEDK
310 320 330 340
DLQLFRKLGL NPQQTATSHG DREQQQALTE QLLHGDTAQF YNAAV
Length:345
Mass (Da):38,378
Last modified:July 5, 2004 - v1
Checksum:i5E947F85B26780A1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009952 Genomic DNA Translation: AAM86582.1
AE017042 Genomic DNA Translation: AAS61260.1
AL590842 Genomic DNA Translation: CAL19815.1
PIRiAE0141
RefSeqiWP_002210762.1, NZ_PDBR01000020.1
YP_002346190.1, NC_003143.1

Genome annotation databases

EnsemblBacteriaiAAM86582; AAM86582; y3031
AAS61260; AAS61260; YP_1009
GeneIDi1174000
KEGGiype:YPO1151
ypk:y3031
ypm:YP_1009
PATRICifig|214092.21.peg.1446

Similar proteinsi

Entry informationi

Entry nameiBIOB_YERPE
AccessioniPrimary (citable) accession number: Q7CH65
Secondary accession number(s): Q74W80
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 5, 2004
Last modified: March 28, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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