Reviewed, UniProtKB/Swiss-Prot Q7CF36 (FTHS1_STRP3)

Names and origin

Protein names

Recommended name:
    Formate--tetrahydrofolate ligase 1
    EC=6.3.4.3
Alternative name(s):
    Formyltetrahydrofolate synthetase 1
      Short name=FHS 1
      Short name=FTHFS 1

Gene names

Name:	fhs1
Synonyms:	fhs
Ordered Locus Names:	SpyM3_0853, SPs1053

Organism

Streptococcus pyogenes serotype M3 [Complete proteome] [HAMAP]

Taxonomic identifier

301448 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	556 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	folic acid and derivative biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 556	556	Formate--tetrahydrofolate ligase 1 HAMAP MF_01543		PRO_0000199395
Regions
	Nucleotide binding	65 – 72	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q7CF36-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 90C6D132DF20EE3C	FASTA	556	59,531
	10 20 30 40 50 60 MKSDIEIAQS VALQPITDIV KKVGIDGDDI ELYGKYKAKL SFEKMKAVEA NEPGKLILVT 70 80 90 100 110 120 AINPTPAGEG KSTMSIGLAD ALNQMGKKTM LALREPSLGP VMGIKGGAAG GGYAQVLPME 130 140 150 160 170 180 DINLHFTGDM HAITTANNAL SALIDNHLQQ GNDLGIDPRR IIWKRVLDLN DRALRQVIVG 190 200 210 220 230 240 LGSPVNGVPR EDGFDITVAS EIMAILCLAT DLKDLKKRLA DIVVAYTYDR KPVYVRDLKV 250 260 270 280 290 300 EGALTLILKD AIKPNLVQTI YGTPALIHGG PFANIAHGCN SVLATSTALR LADYTVTEAG 310 320 330 340 350 360 FGADLGAEKF LNIKVPNLPK APDAIVIVAT LRALKMHGGV AKSDLAAENC EAVRLGFANL 370 380 390 400 410 420 KRHVENMRQF KVPVVVAINE FVADTEAEIA TLKALCEEIK VPVELASVWA NGAEGGLALA 430 440 450 460 470 480 KTVVRVIDQE AADYKRLYSD EDTLEEKVIN IVTQIYGGKA VQFGPKAKTQ LKQFAEFGWD 490 500 510 520 530 540 KLPVCMAKTQ YSFSDNPSLL GAPTDFDITI REFVPKTGAG FIVGLTGDVM TMPGLPKVPA 550 AMAMDVAENG TALGLF

References

[1]

"Genome sequence of a serotype M3 strain of group A Streptococcus: phage-encoded toxins, the high-virulence phenotype, and clone emergence." Beres S.B., Sylva G.L., Barbian K.D., Lei B., Hoff J.S., Mammarella N.D., Liu M.-Y., Smoot J.C., Porcella S.F., Parkins L.D., Campbell D.S., Smith T.M., McCormick J.K., Leung D.Y.M., Schlievert P.M., Musser J.M. Proc. Natl. Acad. Sci. U.S.A. 99:10078-10083(2002) [PubMed: 12122206] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-595 / MGAS315 / Serotype M3.

[2]

"Genome sequence of an M3 strain of Streptococcus pyogenes reveals a large-scale genomic rearrangement in invasive strains and new insights into phage evolution." Nakagawa I., Kurokawa K., Yamashita A., Nakata M., Tomiyasu Y., Okahashi N., Kawabata S., Yamazaki K., Shiba T., Yasunaga T., Hayashi H., Hattori M., Hamada S. Genome Res. 13:1042-1055(2003) [PubMed: 12799345] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SSI-1 / Serotype M3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014074 Genomic DNA. Translation: AAM79460.1. BA000034 Genomic DNA. Translation: BAC64148.1.
RefSeq	NP_664657.1. NP_802315.1.
3D structure databases
SMR	Q7CF36. Positions 4-554.
ModBase	Search...
Genome annotation databases
GeneID	1009167. 1066231.
GenomeReviews	Gene locus SpyM3_0853 in contig AE014074_GR. Gene locus SPs1053 in contig BA000034_GR.
KEGG	spg:SpyM3_0853. sps:SPs1053.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG677721.
OMA	GMKGGAT.
Enzyme and pathway databases
BioCyc	SPYO193567:SPS1053-MONOMER. SPYO198466:SPYM3_0853-MONOMER.
Family and domain databases
HAMAP	MF_01543. FTHFS. [Tree]
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. [Graphical view]
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS1_STRP3

Accession

Primary (citable) accession number: Q7CF36
Secondary accession number(s): Q79X48

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	July 5, 2004
Last modified:	January 19, 2010
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)